Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O08532

- CA2D1_MOUSE

UniProt

O08532 - CA2D1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Voltage-dependent calcium channel subunit alpha-2/delta-1

Gene

Cacna2d1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi259 – 2591Divalent metal cationBy similarity
Metal bindingi261 – 2611Divalent metal cationBy similarity
Metal bindingi263 – 2631Divalent metal cationBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. voltage-gated calcium channel activity Source: Ensembl

GO - Biological processi

  1. regulation of calcium ion transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-1
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-1
Cleaved into the following 2 chains:
Gene namesi
Name:Cacna2d1
Synonyms:Cacna2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:88295. Cacna2d1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 10731049ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1074 – 109421HelicalSequence AnalysisAdd
BLAST
Topological domaini1095 – 11039CytoplasmicSequence Analysis

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. sarcoplasmic reticulum Source: MGI
  3. T-tubule Source: MGI
  4. voltage-gated calcium channel complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424By similarityAdd
BLAST
Chaini25 – 11031079Voltage-dependent calcium channel subunit alpha-2/delta-1PRO_0000304634Add
BLAST
Chaini25 – 957933Voltage-dependent calcium channel subunit alpha-2-1PRO_0000005003Add
BLAST
Chaini958 – 1103146Voltage-dependent calcium channel subunit delta-1PRO_0000005004Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi136 – 1361N-linked (GlcNAc...)2 Publications
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi323 – 3231N-linked (GlcNAc...); atypical1 Publication
Glycosylationi324 – 3241N-linked (GlcNAc...)1 Publication
Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi404 ↔ 1059Interchain (between alpha-2-1 and delta-1 chains)By similarity
Glycosylationi613 – 6131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi772 – 7721N-linked (GlcNAc...); atypical1 Publication
Glycosylationi781 – 7811N-linked (GlcNAc...)2 Publications
Glycosylationi888 – 8881N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Proteolytically processed into subunits alpha-2-1 and delta-1 that are disulfide-linked.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO08532.
PaxDbiO08532.
PRIDEiO08532.

PTM databases

PhosphoSiteiO08532.

Expressioni

Tissue specificityi

Isoform 2A is expressed in skeletal muscle and aorta. Isoform 2B is expressed in brain. Isoform 2C is expressed in heart. Isoform 2D is expressed in heart and smooth muscle. Isoform 2E is expressed in smooth muscle. All five isoforms are expressed in the cardiovascular system.

Gene expression databases

BgeeiO08532.
ExpressionAtlasiO08532. baseline and differential.
GenevestigatoriO08532.

Interactioni

Subunit structurei

Dimer formed of alpha-2-1 and delta-1 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio (By similarity).By similarity

Protein-protein interaction databases

BioGridi198437. 1 interaction.
IntActiO08532. 5 interactions.
MINTiMINT-4089599.

Structurei

3D structure databases

ProteinModelPortaliO08532.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini253 – 430178VWFAPROSITE-ProRule annotationAdd
BLAST
Domaini446 – 53792CacheAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi259 – 2635MIDAS-like motif

Domaini

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.By similarity

Sequence similaritiesi

Contains 1 cache domain.Curated
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG307080.
GeneTreeiENSGT00530000062904.
HOGENOMiHOG000004860.
HOVERGENiHBG057779.
InParanoidiO08532.
KOiK04858.
OMAiDDYTNQI.
OrthoDBiEOG70GMDV.
PhylomeDBiO08532.
TreeFamiTF315824.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR004010. Cache_domain.
IPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamiPF02743. Cache_1. 1 hit.
PF08473. VGCC_alpha2. 1 hit.
PF00092. VWA. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. Align

Isoform 2A (identifier: O08532-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGCLLALT LTLFQSGLIG PSSEEPFPSP VTIKSWVDKM QEDLVTLAKT
60 70 80 90 100
ASGVTQLADI YEKYQDLYTV EPNNARQLVE IAARDIEKLL SNRSKALVRL
110 120 130 140 150
AMEAEKVQAA HQWREDFASN EVVYYNAKDD LDPERNESEP GSQRIKPVFI
160 170 180 190 200
EDANFGRQIS YQHAAVHIPT DIYEGSTIVL NELNWTSALD EVFKRNRDED
210 220 230 240 250
PTLLWQVFGS ATGLARYYPA SPWVDNSRTP NKIDLYDVRR RPWYIQGAAS
260 270 280 290 300
PKDMLILVDV SGSVSGLTLK LIRTSVSEML ETLSDDDFVN VASFNSNAQD
310 320 330 340 350
VSCFQHLVQA NVRNKKVLKD AVNNITAKGI TDYKKGFSFA FEQLLNYNVS
360 370 380 390 400
RANCNKIIML FTDGGEERAQ EIFAKYNKDK KVRVFTFSVG QHNYDRGPIQ
410 420 430 440 450
WMACENKGYY YEIPSIGAIR INTQEYLDVL GRPMVLAGDK AKQVQWTNVY
460 470 480 490 500
LDALELGLVI TGTLPVFNVT GQSENKTNLK NQLILGVMGV DVSLEDIKRL
510 520 530 540 550
TPRFTLCPNG YYFAIDPNGY VLLHPNLQPK PIGVGIPTIN LRKRRPNVQN
560 570 580 590 600
PKSQEPVTLD FLDAELENEI KVEIRNKMID GESGEKTFRT LVKSQDERYI
610 620 630 640 650
DKGNRTYTWT PVNGTDYSLA LVLPTYSFYY IKAKLEETIT QARYSETLKP
660 670 680 690 700
DNFEESGYTF IAPREYCNDL KPSDNNTEFL LNFNEFIDRK TPNNPSCNTD
710 720 730 740 750
LINRILLDAG FTNELVQNYW SKQKNIKGVK ARFVVTDGGI TRVYPKEAGE
760 770 780 790 800
NWQENPETYE DSFYKRSLDN DNYVFTAPYF NKSGPGAYES GIMVSKAVEL
810 820 830 840 850
YIQGKLLKPA VVGIKIDVNS WIENFTKTSI RDPCAGPVCD CKRNSDVMDC
860 870 880 890 900
VILDDGGFLL MANHDDYTNQ IGRFFGEIDP SMMRHLVNIS LYAFNKSYDY
910 920 930 940 950
QSVCDPGAAP KQGAGHRSAY VPSIADILQI GWWATAAAWS ILQQLLLSLT
960 970 980 990 1000
FPRLLEAVEM EEDDFTASLS KQSCITEQTQ YFFKNDTKSF SGLLDCGNCS
1010 1020 1030 1040 1050
RIFHVEKLMN TNLVFIMVES KGTCPCDTRL LMQAEQTSDG PDPCDMVKQP
1060 1070 1080 1090 1100
RYRKGPDVCF DNNVLEDYTD CGGVSGLNPS LWSIFGLQFI LLWLVSGSRH

YLL
Length:1,103
Mass (Da):124,630
Last modified:July 1, 1997 - v1
Checksum:i103773B4735120D4
GO
Isoform 2B (identifier: O08532-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-549: Missing.

Show »
Length:1,084
Mass (Da):122,520
Checksum:i5B9D89AB98F7BD1D
GO
Isoform 2C (identifier: O08532-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-554: Missing.

Show »
Length:1,079
Mass (Da):121,965
Checksum:i2A19773E55B31828
GO
Isoform 2D (identifier: O08532-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-554: Missing.
     644-644: Y → SKKGKMKD

Show »
Length:1,086
Mass (Da):122,705
Checksum:i1576CB411BB1754A
GO
Isoform 2E (identifier: O08532-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-549: Missing.
     644-644: Y → SKKGKMKD

Show »
Length:1,091
Mass (Da):123,260
Checksum:iCB272F8F81982F4B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei531 – 55424Missing in isoform 2C and isoform 2D. CuratedVSP_050445Add
BLAST
Alternative sequencei531 – 54919Missing in isoform 2B and isoform 2E. CuratedVSP_050444Add
BLAST
Alternative sequencei644 – 6441Y → SKKGKMKD in isoform 2D and isoform 2E. CuratedVSP_050446

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73484 mRNA. Translation: AAB50139.1.
U73485 mRNA. Translation: AAB50140.1.
U73483 mRNA. Translation: AAB50138.1.
U73486 mRNA. Translation: AAB50141.1.
U73487 mRNA. Translation: AAB50142.1.
CCDSiCCDS19096.1. [O08532-2]
CCDS51421.1. [O08532-1]
CCDS51422.1. [O08532-5]
CCDS51423.1. [O08532-4]
RefSeqiNP_001104313.1. NM_001110843.1. [O08532-1]
NP_001104314.1. NM_001110844.1. [O08532-5]
NP_001104315.1. NM_001110845.1. [O08532-4]
NP_001104316.1. NM_001110846.1. [O08532-3]
NP_033914.1. NM_009784.2. [O08532-2]
UniGeneiMm.159842.

Genome annotation databases

EnsembliENSMUST00000039370; ENSMUSP00000049457; ENSMUSG00000040118. [O08532-1]
ENSMUST00000078272; ENSMUSP00000077391; ENSMUSG00000040118. [O08532-2]
ENSMUST00000101581; ENSMUSP00000099117; ENSMUSG00000040118. [O08532-5]
ENSMUST00000115281; ENSMUSP00000110936; ENSMUSG00000040118. [O08532-4]
ENSMUST00000180204; ENSMUSP00000136260; ENSMUSG00000040118. [O08532-4]
GeneIDi12293.
KEGGimmu:12293.
UCSCiuc008wmx.2. mouse. [O08532-2]
uc008wmy.2. mouse. [O08532-5]
uc008wna.2. mouse. [O08532-1]
uc008wnb.2. mouse. [O08532-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73484 mRNA. Translation: AAB50139.1 .
U73485 mRNA. Translation: AAB50140.1 .
U73483 mRNA. Translation: AAB50138.1 .
U73486 mRNA. Translation: AAB50141.1 .
U73487 mRNA. Translation: AAB50142.1 .
CCDSi CCDS19096.1. [O08532-2 ]
CCDS51421.1. [O08532-1 ]
CCDS51422.1. [O08532-5 ]
CCDS51423.1. [O08532-4 ]
RefSeqi NP_001104313.1. NM_001110843.1. [O08532-1 ]
NP_001104314.1. NM_001110844.1. [O08532-5 ]
NP_001104315.1. NM_001110845.1. [O08532-4 ]
NP_001104316.1. NM_001110846.1. [O08532-3 ]
NP_033914.1. NM_009784.2. [O08532-2 ]
UniGenei Mm.159842.

3D structure databases

ProteinModelPortali O08532.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198437. 1 interaction.
IntActi O08532. 5 interactions.
MINTi MINT-4089599.

Chemistry

BindingDBi O08532.
ChEMBLi CHEMBL4676.

PTM databases

PhosphoSitei O08532.

Proteomic databases

MaxQBi O08532.
PaxDbi O08532.
PRIDEi O08532.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000039370 ; ENSMUSP00000049457 ; ENSMUSG00000040118 . [O08532-1 ]
ENSMUST00000078272 ; ENSMUSP00000077391 ; ENSMUSG00000040118 . [O08532-2 ]
ENSMUST00000101581 ; ENSMUSP00000099117 ; ENSMUSG00000040118 . [O08532-5 ]
ENSMUST00000115281 ; ENSMUSP00000110936 ; ENSMUSG00000040118 . [O08532-4 ]
ENSMUST00000180204 ; ENSMUSP00000136260 ; ENSMUSG00000040118 . [O08532-4 ]
GeneIDi 12293.
KEGGi mmu:12293.
UCSCi uc008wmx.2. mouse. [O08532-2 ]
uc008wmy.2. mouse. [O08532-5 ]
uc008wna.2. mouse. [O08532-1 ]
uc008wnb.2. mouse. [O08532-3 ]

Organism-specific databases

CTDi 781.
MGIi MGI:88295. Cacna2d1.

Phylogenomic databases

eggNOGi NOG307080.
GeneTreei ENSGT00530000062904.
HOGENOMi HOG000004860.
HOVERGENi HBG057779.
InParanoidi O08532.
KOi K04858.
OMAi DDYTNQI.
OrthoDBi EOG70GMDV.
PhylomeDBi O08532.
TreeFami TF315824.

Miscellaneous databases

ChiTaRSi Cacna2d1. mouse.
NextBioi 280788.
PROi O08532.
SOURCEi Search...

Gene expression databases

Bgeei O08532.
ExpressionAtlasi O08532. baseline and differential.
Genevestigatori O08532.

Family and domain databases

Gene3Di 3.40.50.410. 1 hit.
InterProi IPR004010. Cache_domain.
IPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view ]
Pfami PF02743. Cache_1. 1 hit.
PF08473. VGCC_alpha2. 1 hit.
PF00092. VWA. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view ]
SMARTi SM00327. VWA. 1 hit.
[Graphical view ]
SUPFAMi SSF53300. SSF53300. 1 hit.
PROSITEi PS50234. VWFA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Tissue-specific expression of splice variants of the mouse voltage-gated calcium channel alpha2/delta subunit."
    Angelotti T., Hofmann F.
    FEBS Lett. 397:331-337(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  3. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-136; ASN-323; ASN-324; ASN-772 AND ASN-781.
    Tissue: Myoblast.
  4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-136 AND ASN-781.

Entry informationi

Entry nameiCA2D1_MOUSE
AccessioniPrimary (citable) accession number: O08532
Secondary accession number(s): O08533
, O08534, O08535, O08536
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: July 1, 1997
Last modified: November 26, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds gabapentin, an antiepileptic drug.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3