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O08532 (CA2D1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-1
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-1
Gene names
Name:Cacna2d1
Synonyms:Cacna2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1103 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling By similarity.

Subunit structure

Dimer formed of alpha-2-1 and delta-1 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

Isoform 2A is expressed in skeletal muscle and aorta. Isoform 2B is expressed in brain. Isoform 2C is expressed in heart. Isoform 2D is expressed in heart and smooth muscle. Isoform 2E is expressed in smooth muscle. All five isoforms are expressed in the cardiovascular system.

Domain

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch By similarity.

Post-translational modification

Proteolytically processed into subunits alpha-2-1 and delta-1 that are disulfide-linked By similarity.

Miscellaneous

Binds gabapentin, an antiepileptic drug By similarity.

Sequence similarities

Belongs to the calcium channel subunit alpha-2/delta family.

Contains 1 cache domain.

Contains 1 VWFA domain.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2A (identifier: O08532-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2B (identifier: O08532-2)

The sequence of this isoform differs from the canonical sequence as follows:
     531-549: Missing.
Isoform 2C (identifier: O08532-3)

The sequence of this isoform differs from the canonical sequence as follows:
     531-554: Missing.
Isoform 2D (identifier: O08532-4)

The sequence of this isoform differs from the canonical sequence as follows:
     531-554: Missing.
     644-644: Y → SKKGKMKD
Isoform 2E (identifier: O08532-5)

The sequence of this isoform differs from the canonical sequence as follows:
     531-549: Missing.
     644-644: Y → SKKGKMKD

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 By similarity
Chain25 – 11031079Voltage-dependent calcium channel subunit alpha-2/delta-1
PRO_0000304634
Chain25 – 957933Voltage-dependent calcium channel subunit alpha-2-1
PRO_0000005003
Chain958 – 1103146Voltage-dependent calcium channel subunit delta-1
PRO_0000005004

Regions

Topological domain25 – 10731049Extracellular Potential
Transmembrane1074 – 109421Helical; Potential
Topological domain1095 – 11039Cytoplasmic Potential
Domain253 – 430178VWFA
Domain446 – 53792Cache
Motif259 – 2635MIDAS-like motif

Sites

Metal binding2591Divalent metal cation By similarity
Metal binding2611Divalent metal cation By similarity
Metal binding2631Divalent metal cation By similarity

Amino acid modifications

Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation1361N-linked (GlcNAc...) Ref.3 Ref.4
Glycosylation1841N-linked (GlcNAc...) Potential
Glycosylation3231N-linked (GlcNAc...); atypical Ref.3
Glycosylation3241N-linked (GlcNAc...) Ref.3
Glycosylation3481N-linked (GlcNAc...) Potential
Glycosylation6131N-linked (GlcNAc...) Potential
Glycosylation7721N-linked (GlcNAc...); atypical Ref.3
Glycosylation7811N-linked (GlcNAc...) Ref.3 Ref.4
Glycosylation8881N-linked (GlcNAc...) Potential
Disulfide bond404 ↔ 1059Interchain (between alpha-2-1 and delta-1 chains) By similarity

Natural variations

Alternative sequence531 – 55424Missing in isoform 2C and isoform 2D.
VSP_050445
Alternative sequence531 – 54919Missing in isoform 2B and isoform 2E.
VSP_050444
Alternative sequence6441Y → SKKGKMKD in isoform 2D and isoform 2E.
VSP_050446

Sequences

Sequence LengthMass (Da)Tools
Isoform 2A [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 103773B4735120D4

FASTA1,103124,630
        10         20         30         40         50         60 
MAAGCLLALT LTLFQSGLIG PSSEEPFPSP VTIKSWVDKM QEDLVTLAKT ASGVTQLADI 

        70         80         90        100        110        120 
YEKYQDLYTV EPNNARQLVE IAARDIEKLL SNRSKALVRL AMEAEKVQAA HQWREDFASN 

       130        140        150        160        170        180 
EVVYYNAKDD LDPERNESEP GSQRIKPVFI EDANFGRQIS YQHAAVHIPT DIYEGSTIVL 

       190        200        210        220        230        240 
NELNWTSALD EVFKRNRDED PTLLWQVFGS ATGLARYYPA SPWVDNSRTP NKIDLYDVRR 

       250        260        270        280        290        300 
RPWYIQGAAS PKDMLILVDV SGSVSGLTLK LIRTSVSEML ETLSDDDFVN VASFNSNAQD 

       310        320        330        340        350        360 
VSCFQHLVQA NVRNKKVLKD AVNNITAKGI TDYKKGFSFA FEQLLNYNVS RANCNKIIML 

       370        380        390        400        410        420 
FTDGGEERAQ EIFAKYNKDK KVRVFTFSVG QHNYDRGPIQ WMACENKGYY YEIPSIGAIR 

       430        440        450        460        470        480 
INTQEYLDVL GRPMVLAGDK AKQVQWTNVY LDALELGLVI TGTLPVFNVT GQSENKTNLK 

       490        500        510        520        530        540 
NQLILGVMGV DVSLEDIKRL TPRFTLCPNG YYFAIDPNGY VLLHPNLQPK PIGVGIPTIN 

       550        560        570        580        590        600 
LRKRRPNVQN PKSQEPVTLD FLDAELENEI KVEIRNKMID GESGEKTFRT LVKSQDERYI 

       610        620        630        640        650        660 
DKGNRTYTWT PVNGTDYSLA LVLPTYSFYY IKAKLEETIT QARYSETLKP DNFEESGYTF 

       670        680        690        700        710        720 
IAPREYCNDL KPSDNNTEFL LNFNEFIDRK TPNNPSCNTD LINRILLDAG FTNELVQNYW 

       730        740        750        760        770        780 
SKQKNIKGVK ARFVVTDGGI TRVYPKEAGE NWQENPETYE DSFYKRSLDN DNYVFTAPYF 

       790        800        810        820        830        840 
NKSGPGAYES GIMVSKAVEL YIQGKLLKPA VVGIKIDVNS WIENFTKTSI RDPCAGPVCD 

       850        860        870        880        890        900 
CKRNSDVMDC VILDDGGFLL MANHDDYTNQ IGRFFGEIDP SMMRHLVNIS LYAFNKSYDY 

       910        920        930        940        950        960 
QSVCDPGAAP KQGAGHRSAY VPSIADILQI GWWATAAAWS ILQQLLLSLT FPRLLEAVEM 

       970        980        990       1000       1010       1020 
EEDDFTASLS KQSCITEQTQ YFFKNDTKSF SGLLDCGNCS RIFHVEKLMN TNLVFIMVES 

      1030       1040       1050       1060       1070       1080 
KGTCPCDTRL LMQAEQTSDG PDPCDMVKQP RYRKGPDVCF DNNVLEDYTD CGGVSGLNPS 

      1090       1100 
LWSIFGLQFI LLWLVSGSRH YLL 

« Hide

Isoform 2B [UniParc].

Checksum: 5B9D89AB98F7BD1D
Show »

FASTA1,084122,520
Isoform 2C [UniParc].

Checksum: 2A19773E55B31828
Show »

FASTA1,079121,965
Isoform 2D [UniParc].

Checksum: 1576CB411BB1754A
Show »

FASTA1,086122,705
Isoform 2E [UniParc].

Checksum: CB272F8F81982F4B
Show »

FASTA1,091123,260

References

« Hide 'large scale' references
[1]"Tissue-specific expression of splice variants of the mouse voltage-gated calcium channel alpha2/delta subunit."
Angelotti T., Hofmann F.
FEBS Lett. 397:331-337(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[3]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-136; ASN-323; ASN-324; ASN-772 AND ASN-781.
Tissue: Myoblast.
[4]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-136 AND ASN-781.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U73484 mRNA. Translation: AAB50139.1.
U73485 mRNA. Translation: AAB50140.1.
U73483 mRNA. Translation: AAB50138.1.
U73486 mRNA. Translation: AAB50141.1.
U73487 mRNA. Translation: AAB50142.1.
RefSeqNP_001104313.1. NM_001110843.1.
NP_001104314.1. NM_001110844.1.
NP_001104315.1. NM_001110845.1.
NP_001104316.1. NM_001110846.1.
NP_033914.1. NM_009784.2.
UniGeneMm.159842.

3D structure databases

ProteinModelPortalO08532.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198437. 1 interaction.
IntActO08532. 5 interactions.
MINTMINT-4089599.

Chemistry

BindingDBO08532.
ChEMBLCHEMBL4676.

PTM databases

PhosphoSiteO08532.

Proteomic databases

PaxDbO08532.
PRIDEO08532.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000039370; ENSMUSP00000049457; ENSMUSG00000040118. [O08532-1]
ENSMUST00000078272; ENSMUSP00000077391; ENSMUSG00000040118. [O08532-2]
ENSMUST00000101581; ENSMUSP00000099117; ENSMUSG00000040118. [O08532-5]
ENSMUST00000115281; ENSMUSP00000110936; ENSMUSG00000040118. [O08532-4]
ENSMUST00000180204; ENSMUSP00000136260; ENSMUSG00000040118. [O08532-4]
GeneID12293.
KEGGmmu:12293.
UCSCuc008wmx.2. mouse. [O08532-2]
uc008wmy.2. mouse. [O08532-5]
uc008wna.2. mouse. [O08532-1]
uc008wnb.2. mouse. [O08532-3]

Organism-specific databases

CTD781.
MGIMGI:88295. Cacna2d1.

Phylogenomic databases

eggNOGNOG307080.
GeneTreeENSGT00530000062904.
HOGENOMHOG000004860.
HOVERGENHBG057779.
InParanoidO08532.
KOK04858.
OMADDYTNQI.
OrthoDBEOG70GMDV.
PhylomeDBO08532.
TreeFamTF315824.

Gene expression databases

ArrayExpressO08532.
BgeeO08532.
GenevestigatorO08532.

Family and domain databases

Gene3D3.40.50.410. 1 hit.
InterProIPR004010. Cache_domain.
IPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamPF02743. Cache_1. 1 hit.
PF08473. VGCC_alpha2. 1 hit.
PF00092. VWA. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMSSF53300. SSF53300. 1 hit.
PROSITEPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCACNA2D1. mouse.
NextBio280788.
PROO08532.
SOURCESearch...

Entry information

Entry nameCA2D1_MOUSE
AccessionPrimary (citable) accession number: O08532
Secondary accession number(s): O08533 expand/collapse secondary AC list , O08534, O08535, O08536
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot