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O08532

- CA2D1_MOUSE

UniProt

O08532 - CA2D1_MOUSE

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Protein
Voltage-dependent calcium channel subunit alpha-2/delta-1
Gene
Cacna2d1, Cacna2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi259 – 2591Divalent metal cation By similarity
Metal bindingi261 – 2611Divalent metal cation By similarity
Metal bindingi263 – 2631Divalent metal cation By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein binding Source: MGI
  3. voltage-gated calcium channel activity Source: Ensembl
Complete GO annotation...

GO - Biological processi

  1. regulation of calcium ion transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-1
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-1
Cleaved into the following 2 chains:
Gene namesi
Name:Cacna2d1
Synonyms:Cacna2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:88295. Cacna2d1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 10731049Extracellular Reviewed prediction
Add
BLAST
Transmembranei1074 – 109421Helical; Reviewed prediction
Add
BLAST
Topological domaini1095 – 11039Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. T-tubule Source: MGI
  2. sarcoplasmic reticulum Source: MGI
  3. voltage-gated calcium channel complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 By similarity
Add
BLAST
Chaini25 – 11031079Voltage-dependent calcium channel subunit alpha-2/delta-1
PRO_0000304634Add
BLAST
Chaini25 – 957933Voltage-dependent calcium channel subunit alpha-2-1
PRO_0000005003Add
BLAST
Chaini958 – 1103146Voltage-dependent calcium channel subunit delta-1
PRO_0000005004Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi92 – 921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi136 – 1361N-linked (GlcNAc...)2 Publications
Glycosylationi184 – 1841N-linked (GlcNAc...) Reviewed prediction
Glycosylationi323 – 3231N-linked (GlcNAc...); atypical1 Publication
Glycosylationi324 – 3241N-linked (GlcNAc...)1 Publication
Glycosylationi348 – 3481N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi404 ↔ 1059Interchain (between alpha-2-1 and delta-1 chains) By similarity
Glycosylationi613 – 6131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi772 – 7721N-linked (GlcNAc...); atypical1 Publication
Glycosylationi781 – 7811N-linked (GlcNAc...)2 Publications
Glycosylationi888 – 8881N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Proteolytically processed into subunits alpha-2-1 and delta-1 that are disulfide-linked By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO08532.
PaxDbiO08532.
PRIDEiO08532.

PTM databases

PhosphoSiteiO08532.

Expressioni

Tissue specificityi

Isoform 2A is expressed in skeletal muscle and aorta. Isoform 2B is expressed in brain. Isoform 2C is expressed in heart. Isoform 2D is expressed in heart and smooth muscle. Isoform 2E is expressed in smooth muscle. All five isoforms are expressed in the cardiovascular system.

Gene expression databases

ArrayExpressiO08532.
BgeeiO08532.
GenevestigatoriO08532.

Interactioni

Subunit structurei

Dimer formed of alpha-2-1 and delta-1 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio By similarity.

Protein-protein interaction databases

BioGridi198437. 1 interaction.
IntActiO08532. 5 interactions.
MINTiMINT-4089599.

Structurei

3D structure databases

ProteinModelPortaliO08532.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini253 – 430178VWFA
Add
BLAST
Domaini446 – 53792Cache
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi259 – 2635MIDAS-like motif

Domaini

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch By similarity.

Sequence similaritiesi

Contains 1 cache domain.
Contains 1 VWFA domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG307080.
GeneTreeiENSGT00530000062904.
HOGENOMiHOG000004860.
HOVERGENiHBG057779.
InParanoidiO08532.
KOiK04858.
OMAiDDYTNQI.
OrthoDBiEOG70GMDV.
PhylomeDBiO08532.
TreeFamiTF315824.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR004010. Cache_domain.
IPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamiPF02743. Cache_1. 1 hit.
PF08473. VGCC_alpha2. 1 hit.
PF00092. VWA. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. Align

Isoform 2A (identifier: O08532-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAAGCLLALT LTLFQSGLIG PSSEEPFPSP VTIKSWVDKM QEDLVTLAKT     50
ASGVTQLADI YEKYQDLYTV EPNNARQLVE IAARDIEKLL SNRSKALVRL 100
AMEAEKVQAA HQWREDFASN EVVYYNAKDD LDPERNESEP GSQRIKPVFI 150
EDANFGRQIS YQHAAVHIPT DIYEGSTIVL NELNWTSALD EVFKRNRDED 200
PTLLWQVFGS ATGLARYYPA SPWVDNSRTP NKIDLYDVRR RPWYIQGAAS 250
PKDMLILVDV SGSVSGLTLK LIRTSVSEML ETLSDDDFVN VASFNSNAQD 300
VSCFQHLVQA NVRNKKVLKD AVNNITAKGI TDYKKGFSFA FEQLLNYNVS 350
RANCNKIIML FTDGGEERAQ EIFAKYNKDK KVRVFTFSVG QHNYDRGPIQ 400
WMACENKGYY YEIPSIGAIR INTQEYLDVL GRPMVLAGDK AKQVQWTNVY 450
LDALELGLVI TGTLPVFNVT GQSENKTNLK NQLILGVMGV DVSLEDIKRL 500
TPRFTLCPNG YYFAIDPNGY VLLHPNLQPK PIGVGIPTIN LRKRRPNVQN 550
PKSQEPVTLD FLDAELENEI KVEIRNKMID GESGEKTFRT LVKSQDERYI 600
DKGNRTYTWT PVNGTDYSLA LVLPTYSFYY IKAKLEETIT QARYSETLKP 650
DNFEESGYTF IAPREYCNDL KPSDNNTEFL LNFNEFIDRK TPNNPSCNTD 700
LINRILLDAG FTNELVQNYW SKQKNIKGVK ARFVVTDGGI TRVYPKEAGE 750
NWQENPETYE DSFYKRSLDN DNYVFTAPYF NKSGPGAYES GIMVSKAVEL 800
YIQGKLLKPA VVGIKIDVNS WIENFTKTSI RDPCAGPVCD CKRNSDVMDC 850
VILDDGGFLL MANHDDYTNQ IGRFFGEIDP SMMRHLVNIS LYAFNKSYDY 900
QSVCDPGAAP KQGAGHRSAY VPSIADILQI GWWATAAAWS ILQQLLLSLT 950
FPRLLEAVEM EEDDFTASLS KQSCITEQTQ YFFKNDTKSF SGLLDCGNCS 1000
RIFHVEKLMN TNLVFIMVES KGTCPCDTRL LMQAEQTSDG PDPCDMVKQP 1050
RYRKGPDVCF DNNVLEDYTD CGGVSGLNPS LWSIFGLQFI LLWLVSGSRH 1100
YLL 1103
Length:1,103
Mass (Da):124,630
Last modified:July 1, 1997 - v1
Checksum:i103773B4735120D4
GO
Isoform 2B (identifier: O08532-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-549: Missing.

Show »
Length:1,084
Mass (Da):122,520
Checksum:i5B9D89AB98F7BD1D
GO
Isoform 2C (identifier: O08532-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-554: Missing.

Show »
Length:1,079
Mass (Da):121,965
Checksum:i2A19773E55B31828
GO
Isoform 2D (identifier: O08532-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-554: Missing.
     644-644: Y → SKKGKMKD

Show »
Length:1,086
Mass (Da):122,705
Checksum:i1576CB411BB1754A
GO
Isoform 2E (identifier: O08532-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-549: Missing.
     644-644: Y → SKKGKMKD

Show »
Length:1,091
Mass (Da):123,260
Checksum:iCB272F8F81982F4B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei531 – 55424Missing in isoform 2C and isoform 2D.
VSP_050445Add
BLAST
Alternative sequencei531 – 54919Missing in isoform 2B and isoform 2E.
VSP_050444Add
BLAST
Alternative sequencei644 – 6441Y → SKKGKMKD in isoform 2D and isoform 2E.
VSP_050446

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73484 mRNA. Translation: AAB50139.1.
U73485 mRNA. Translation: AAB50140.1.
U73483 mRNA. Translation: AAB50138.1.
U73486 mRNA. Translation: AAB50141.1.
U73487 mRNA. Translation: AAB50142.1.
CCDSiCCDS19096.1. [O08532-2]
CCDS51421.1. [O08532-1]
CCDS51422.1. [O08532-5]
CCDS51423.1. [O08532-4]
RefSeqiNP_001104313.1. NM_001110843.1. [O08532-1]
NP_001104314.1. NM_001110844.1. [O08532-5]
NP_001104315.1. NM_001110845.1. [O08532-4]
NP_001104316.1. NM_001110846.1. [O08532-3]
NP_033914.1. NM_009784.2. [O08532-2]
UniGeneiMm.159842.

Genome annotation databases

EnsembliENSMUST00000039370; ENSMUSP00000049457; ENSMUSG00000040118. [O08532-1]
ENSMUST00000078272; ENSMUSP00000077391; ENSMUSG00000040118. [O08532-2]
ENSMUST00000101581; ENSMUSP00000099117; ENSMUSG00000040118. [O08532-5]
ENSMUST00000115281; ENSMUSP00000110936; ENSMUSG00000040118. [O08532-4]
ENSMUST00000180204; ENSMUSP00000136260; ENSMUSG00000040118. [O08532-4]
GeneIDi12293.
KEGGimmu:12293.
UCSCiuc008wmx.2. mouse. [O08532-2]
uc008wmy.2. mouse. [O08532-5]
uc008wna.2. mouse. [O08532-1]
uc008wnb.2. mouse. [O08532-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73484 mRNA. Translation: AAB50139.1 .
U73485 mRNA. Translation: AAB50140.1 .
U73483 mRNA. Translation: AAB50138.1 .
U73486 mRNA. Translation: AAB50141.1 .
U73487 mRNA. Translation: AAB50142.1 .
CCDSi CCDS19096.1. [O08532-2 ]
CCDS51421.1. [O08532-1 ]
CCDS51422.1. [O08532-5 ]
CCDS51423.1. [O08532-4 ]
RefSeqi NP_001104313.1. NM_001110843.1. [O08532-1 ]
NP_001104314.1. NM_001110844.1. [O08532-5 ]
NP_001104315.1. NM_001110845.1. [O08532-4 ]
NP_001104316.1. NM_001110846.1. [O08532-3 ]
NP_033914.1. NM_009784.2. [O08532-2 ]
UniGenei Mm.159842.

3D structure databases

ProteinModelPortali O08532.
ModBasei Search...

Protein-protein interaction databases

BioGridi 198437. 1 interaction.
IntActi O08532. 5 interactions.
MINTi MINT-4089599.

Chemistry

BindingDBi O08532.
ChEMBLi CHEMBL4676.

PTM databases

PhosphoSitei O08532.

Proteomic databases

MaxQBi O08532.
PaxDbi O08532.
PRIDEi O08532.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000039370 ; ENSMUSP00000049457 ; ENSMUSG00000040118 . [O08532-1 ]
ENSMUST00000078272 ; ENSMUSP00000077391 ; ENSMUSG00000040118 . [O08532-2 ]
ENSMUST00000101581 ; ENSMUSP00000099117 ; ENSMUSG00000040118 . [O08532-5 ]
ENSMUST00000115281 ; ENSMUSP00000110936 ; ENSMUSG00000040118 . [O08532-4 ]
ENSMUST00000180204 ; ENSMUSP00000136260 ; ENSMUSG00000040118 . [O08532-4 ]
GeneIDi 12293.
KEGGi mmu:12293.
UCSCi uc008wmx.2. mouse. [O08532-2 ]
uc008wmy.2. mouse. [O08532-5 ]
uc008wna.2. mouse. [O08532-1 ]
uc008wnb.2. mouse. [O08532-3 ]

Organism-specific databases

CTDi 781.
MGIi MGI:88295. Cacna2d1.

Phylogenomic databases

eggNOGi NOG307080.
GeneTreei ENSGT00530000062904.
HOGENOMi HOG000004860.
HOVERGENi HBG057779.
InParanoidi O08532.
KOi K04858.
OMAi DDYTNQI.
OrthoDBi EOG70GMDV.
PhylomeDBi O08532.
TreeFami TF315824.

Miscellaneous databases

ChiTaRSi CACNA2D1. mouse.
NextBioi 280788.
PROi O08532.
SOURCEi Search...

Gene expression databases

ArrayExpressi O08532.
Bgeei O08532.
Genevestigatori O08532.

Family and domain databases

Gene3Di 3.40.50.410. 1 hit.
InterProi IPR004010. Cache_domain.
IPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view ]
Pfami PF02743. Cache_1. 1 hit.
PF08473. VGCC_alpha2. 1 hit.
PF00092. VWA. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view ]
SMARTi SM00327. VWA. 1 hit.
[Graphical view ]
SUPFAMi SSF53300. SSF53300. 1 hit.
PROSITEi PS50234. VWFA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Tissue-specific expression of splice variants of the mouse voltage-gated calcium channel alpha2/delta subunit."
    Angelotti T., Hofmann F.
    FEBS Lett. 397:331-337(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  3. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-136; ASN-323; ASN-324; ASN-772 AND ASN-781.
    Tissue: Myoblast.
  4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-136 AND ASN-781.

Entry informationi

Entry nameiCA2D1_MOUSE
AccessioniPrimary (citable) accession number: O08532
Secondary accession number(s): O08533
, O08534, O08535, O08536
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds gabapentin, an antiepileptic drug By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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