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O08532

- CA2D1_MOUSE

UniProt

O08532 - CA2D1_MOUSE

Protein

Voltage-dependent calcium channel subunit alpha-2/delta-1

Gene

Cacna2d1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi259 – 2591Divalent metal cationBy similarity
    Metal bindingi261 – 2611Divalent metal cationBy similarity
    Metal bindingi263 – 2631Divalent metal cationBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: MGI
    3. voltage-gated calcium channel activity Source: Ensembl

    GO - Biological processi

    1. regulation of calcium ion transport Source: MGI

    Keywords - Molecular functioni

    Calcium channel, Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Voltage-dependent calcium channel subunit alpha-2/delta-1
    Alternative name(s):
    Voltage-gated calcium channel subunit alpha-2/delta-1
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Cacna2d1
    Synonyms:Cacna2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:88295. Cacna2d1.

    Subcellular locationi

    GO - Cellular componenti

    1. sarcoplasmic reticulum Source: MGI
    2. T-tubule Source: MGI
    3. voltage-gated calcium channel complex Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424By similarityAdd
    BLAST
    Chaini25 – 11031079Voltage-dependent calcium channel subunit alpha-2/delta-1PRO_0000304634Add
    BLAST
    Chaini25 – 957933Voltage-dependent calcium channel subunit alpha-2-1PRO_0000005003Add
    BLAST
    Chaini958 – 1103146Voltage-dependent calcium channel subunit delta-1PRO_0000005004Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi136 – 1361N-linked (GlcNAc...)2 Publications
    Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi323 – 3231N-linked (GlcNAc...); atypical1 Publication
    Glycosylationi324 – 3241N-linked (GlcNAc...)1 Publication
    Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi404 ↔ 1059Interchain (between alpha-2-1 and delta-1 chains)By similarity
    Glycosylationi613 – 6131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi772 – 7721N-linked (GlcNAc...); atypical1 Publication
    Glycosylationi781 – 7811N-linked (GlcNAc...)2 Publications
    Glycosylationi888 – 8881N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Proteolytically processed into subunits alpha-2-1 and delta-1 that are disulfide-linked.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiO08532.
    PaxDbiO08532.
    PRIDEiO08532.

    PTM databases

    PhosphoSiteiO08532.

    Expressioni

    Tissue specificityi

    Isoform 2A is expressed in skeletal muscle and aorta. Isoform 2B is expressed in brain. Isoform 2C is expressed in heart. Isoform 2D is expressed in heart and smooth muscle. Isoform 2E is expressed in smooth muscle. All five isoforms are expressed in the cardiovascular system.

    Gene expression databases

    ArrayExpressiO08532.
    BgeeiO08532.
    GenevestigatoriO08532.

    Interactioni

    Subunit structurei

    Dimer formed of alpha-2-1 and delta-1 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio By similarity.By similarity

    Protein-protein interaction databases

    BioGridi198437. 1 interaction.
    IntActiO08532. 5 interactions.
    MINTiMINT-4089599.

    Structurei

    3D structure databases

    ProteinModelPortaliO08532.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 10731049ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1095 – 11039CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1074 – 109421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini253 – 430178VWFAPROSITE-ProRule annotationAdd
    BLAST
    Domaini446 – 53792CacheAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi259 – 2635MIDAS-like motif

    Domaini

    The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.By similarity

    Sequence similaritiesi

    Contains 1 cache domain.Curated
    Contains 1 VWFA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG307080.
    GeneTreeiENSGT00530000062904.
    HOGENOMiHOG000004860.
    HOVERGENiHBG057779.
    InParanoidiO08532.
    KOiK04858.
    OMAiDDYTNQI.
    OrthoDBiEOG70GMDV.
    PhylomeDBiO08532.
    TreeFamiTF315824.

    Family and domain databases

    Gene3Di3.40.50.410. 1 hit.
    InterProiIPR004010. Cache_domain.
    IPR013680. VDCC_a2/dsu.
    IPR013608. VWA_N.
    IPR002035. VWF_A.
    [Graphical view]
    PfamiPF02743. Cache_1. 1 hit.
    PF08473. VGCC_alpha2. 1 hit.
    PF00092. VWA. 1 hit.
    PF08399. VWA_N. 1 hit.
    [Graphical view]
    SMARTiSM00327. VWA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53300. SSF53300. 1 hit.
    PROSITEiPS50234. VWFA. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Isoform 2A (identifier: O08532-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAGCLLALT LTLFQSGLIG PSSEEPFPSP VTIKSWVDKM QEDLVTLAKT     50
    ASGVTQLADI YEKYQDLYTV EPNNARQLVE IAARDIEKLL SNRSKALVRL 100
    AMEAEKVQAA HQWREDFASN EVVYYNAKDD LDPERNESEP GSQRIKPVFI 150
    EDANFGRQIS YQHAAVHIPT DIYEGSTIVL NELNWTSALD EVFKRNRDED 200
    PTLLWQVFGS ATGLARYYPA SPWVDNSRTP NKIDLYDVRR RPWYIQGAAS 250
    PKDMLILVDV SGSVSGLTLK LIRTSVSEML ETLSDDDFVN VASFNSNAQD 300
    VSCFQHLVQA NVRNKKVLKD AVNNITAKGI TDYKKGFSFA FEQLLNYNVS 350
    RANCNKIIML FTDGGEERAQ EIFAKYNKDK KVRVFTFSVG QHNYDRGPIQ 400
    WMACENKGYY YEIPSIGAIR INTQEYLDVL GRPMVLAGDK AKQVQWTNVY 450
    LDALELGLVI TGTLPVFNVT GQSENKTNLK NQLILGVMGV DVSLEDIKRL 500
    TPRFTLCPNG YYFAIDPNGY VLLHPNLQPK PIGVGIPTIN LRKRRPNVQN 550
    PKSQEPVTLD FLDAELENEI KVEIRNKMID GESGEKTFRT LVKSQDERYI 600
    DKGNRTYTWT PVNGTDYSLA LVLPTYSFYY IKAKLEETIT QARYSETLKP 650
    DNFEESGYTF IAPREYCNDL KPSDNNTEFL LNFNEFIDRK TPNNPSCNTD 700
    LINRILLDAG FTNELVQNYW SKQKNIKGVK ARFVVTDGGI TRVYPKEAGE 750
    NWQENPETYE DSFYKRSLDN DNYVFTAPYF NKSGPGAYES GIMVSKAVEL 800
    YIQGKLLKPA VVGIKIDVNS WIENFTKTSI RDPCAGPVCD CKRNSDVMDC 850
    VILDDGGFLL MANHDDYTNQ IGRFFGEIDP SMMRHLVNIS LYAFNKSYDY 900
    QSVCDPGAAP KQGAGHRSAY VPSIADILQI GWWATAAAWS ILQQLLLSLT 950
    FPRLLEAVEM EEDDFTASLS KQSCITEQTQ YFFKNDTKSF SGLLDCGNCS 1000
    RIFHVEKLMN TNLVFIMVES KGTCPCDTRL LMQAEQTSDG PDPCDMVKQP 1050
    RYRKGPDVCF DNNVLEDYTD CGGVSGLNPS LWSIFGLQFI LLWLVSGSRH 1100
    YLL 1103
    Length:1,103
    Mass (Da):124,630
    Last modified:July 1, 1997 - v1
    Checksum:i103773B4735120D4
    GO
    Isoform 2B (identifier: O08532-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         531-549: Missing.

    Show »
    Length:1,084
    Mass (Da):122,520
    Checksum:i5B9D89AB98F7BD1D
    GO
    Isoform 2C (identifier: O08532-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         531-554: Missing.

    Show »
    Length:1,079
    Mass (Da):121,965
    Checksum:i2A19773E55B31828
    GO
    Isoform 2D (identifier: O08532-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         531-554: Missing.
         644-644: Y → SKKGKMKD

    Show »
    Length:1,086
    Mass (Da):122,705
    Checksum:i1576CB411BB1754A
    GO
    Isoform 2E (identifier: O08532-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         531-549: Missing.
         644-644: Y → SKKGKMKD

    Show »
    Length:1,091
    Mass (Da):123,260
    Checksum:iCB272F8F81982F4B
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei531 – 55424Missing in isoform 2C and isoform 2D. CuratedVSP_050445Add
    BLAST
    Alternative sequencei531 – 54919Missing in isoform 2B and isoform 2E. CuratedVSP_050444Add
    BLAST
    Alternative sequencei644 – 6441Y → SKKGKMKD in isoform 2D and isoform 2E. CuratedVSP_050446

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73484 mRNA. Translation: AAB50139.1.
    U73485 mRNA. Translation: AAB50140.1.
    U73483 mRNA. Translation: AAB50138.1.
    U73486 mRNA. Translation: AAB50141.1.
    U73487 mRNA. Translation: AAB50142.1.
    CCDSiCCDS19096.1. [O08532-2]
    CCDS51421.1. [O08532-1]
    CCDS51422.1. [O08532-5]
    CCDS51423.1. [O08532-4]
    RefSeqiNP_001104313.1. NM_001110843.1. [O08532-1]
    NP_001104314.1. NM_001110844.1. [O08532-5]
    NP_001104315.1. NM_001110845.1. [O08532-4]
    NP_001104316.1. NM_001110846.1. [O08532-3]
    NP_033914.1. NM_009784.2. [O08532-2]
    UniGeneiMm.159842.

    Genome annotation databases

    EnsembliENSMUST00000039370; ENSMUSP00000049457; ENSMUSG00000040118. [O08532-1]
    ENSMUST00000078272; ENSMUSP00000077391; ENSMUSG00000040118. [O08532-2]
    ENSMUST00000101581; ENSMUSP00000099117; ENSMUSG00000040118. [O08532-5]
    ENSMUST00000115281; ENSMUSP00000110936; ENSMUSG00000040118. [O08532-4]
    ENSMUST00000180204; ENSMUSP00000136260; ENSMUSG00000040118. [O08532-4]
    GeneIDi12293.
    KEGGimmu:12293.
    UCSCiuc008wmx.2. mouse. [O08532-2]
    uc008wmy.2. mouse. [O08532-5]
    uc008wna.2. mouse. [O08532-1]
    uc008wnb.2. mouse. [O08532-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73484 mRNA. Translation: AAB50139.1 .
    U73485 mRNA. Translation: AAB50140.1 .
    U73483 mRNA. Translation: AAB50138.1 .
    U73486 mRNA. Translation: AAB50141.1 .
    U73487 mRNA. Translation: AAB50142.1 .
    CCDSi CCDS19096.1. [O08532-2 ]
    CCDS51421.1. [O08532-1 ]
    CCDS51422.1. [O08532-5 ]
    CCDS51423.1. [O08532-4 ]
    RefSeqi NP_001104313.1. NM_001110843.1. [O08532-1 ]
    NP_001104314.1. NM_001110844.1. [O08532-5 ]
    NP_001104315.1. NM_001110845.1. [O08532-4 ]
    NP_001104316.1. NM_001110846.1. [O08532-3 ]
    NP_033914.1. NM_009784.2. [O08532-2 ]
    UniGenei Mm.159842.

    3D structure databases

    ProteinModelPortali O08532.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198437. 1 interaction.
    IntActi O08532. 5 interactions.
    MINTi MINT-4089599.

    Chemistry

    BindingDBi O08532.
    ChEMBLi CHEMBL4676.

    PTM databases

    PhosphoSitei O08532.

    Proteomic databases

    MaxQBi O08532.
    PaxDbi O08532.
    PRIDEi O08532.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039370 ; ENSMUSP00000049457 ; ENSMUSG00000040118 . [O08532-1 ]
    ENSMUST00000078272 ; ENSMUSP00000077391 ; ENSMUSG00000040118 . [O08532-2 ]
    ENSMUST00000101581 ; ENSMUSP00000099117 ; ENSMUSG00000040118 . [O08532-5 ]
    ENSMUST00000115281 ; ENSMUSP00000110936 ; ENSMUSG00000040118 . [O08532-4 ]
    ENSMUST00000180204 ; ENSMUSP00000136260 ; ENSMUSG00000040118 . [O08532-4 ]
    GeneIDi 12293.
    KEGGi mmu:12293.
    UCSCi uc008wmx.2. mouse. [O08532-2 ]
    uc008wmy.2. mouse. [O08532-5 ]
    uc008wna.2. mouse. [O08532-1 ]
    uc008wnb.2. mouse. [O08532-3 ]

    Organism-specific databases

    CTDi 781.
    MGIi MGI:88295. Cacna2d1.

    Phylogenomic databases

    eggNOGi NOG307080.
    GeneTreei ENSGT00530000062904.
    HOGENOMi HOG000004860.
    HOVERGENi HBG057779.
    InParanoidi O08532.
    KOi K04858.
    OMAi DDYTNQI.
    OrthoDBi EOG70GMDV.
    PhylomeDBi O08532.
    TreeFami TF315824.

    Miscellaneous databases

    ChiTaRSi CACNA2D1. mouse.
    NextBioi 280788.
    PROi O08532.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08532.
    Bgeei O08532.
    Genevestigatori O08532.

    Family and domain databases

    Gene3Di 3.40.50.410. 1 hit.
    InterProi IPR004010. Cache_domain.
    IPR013680. VDCC_a2/dsu.
    IPR013608. VWA_N.
    IPR002035. VWF_A.
    [Graphical view ]
    Pfami PF02743. Cache_1. 1 hit.
    PF08473. VGCC_alpha2. 1 hit.
    PF00092. VWA. 1 hit.
    PF08399. VWA_N. 1 hit.
    [Graphical view ]
    SMARTi SM00327. VWA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53300. SSF53300. 1 hit.
    PROSITEi PS50234. VWFA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tissue-specific expression of splice variants of the mouse voltage-gated calcium channel alpha2/delta subunit."
      Angelotti T., Hofmann F.
      FEBS Lett. 397:331-337(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    3. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
      Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
      Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-136; ASN-323; ASN-324; ASN-772 AND ASN-781.
      Tissue: Myoblast.
    4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-136 AND ASN-781.

    Entry informationi

    Entry nameiCA2D1_MOUSE
    AccessioniPrimary (citable) accession number: O08532
    Secondary accession number(s): O08533
    , O08534, O08535, O08536
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2004
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Binds gabapentin, an antiepileptic drug.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3