Reviewed, UniProtKB/Swiss-Prot O08530 (S1PR1_MOUSE)

Names and origin

Protein names

Recommended name:
    Sphingosine 1-phosphate receptor 1
      Short name=S1P receptor 1
      Short name=S1P1
Alternative name(s):
    Sphingosine 1-phosphate receptor Edg-1
      Short name=S1P receptor Edg-1
    Endothelial differentiation G-protein coupled receptor 1
    Lysophospholipid receptor B1

Gene names

Name:	S1pr1
Synonyms:	Edg1, Lpb1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in a wide variety of tissues with highest levels in brain, heart and spleen. Lower levels found in kidney, liver, lung, muscle, placenta, thymus, and uterus. Very low levels in intestine, stomach and testis. According to Ref.2, expressed modestly in apparent endothelial cells surrounding some blood vessels (e.g. aortic trunk). Ref.2
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	angiogenesis Inferred from direct assay. Source: MGI brain development Inferred from mutant phenotype. Source: MGI inhibition of adenylate cyclase activity by G-protein signaling Inferred from direct assay. Source: MGI regulation of cell adhesion Inferred from direct assay. Source: MGI
Cellular component	external side of plasma membrane Inferred from mutant phenotype. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lysosphingolipid and lysophosphatidic acid receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Sphingosine 1-phosphate receptor 1		PRO_0000069413
Regions
	Topological domain	1 – 46	46	Extracellular By similarity
	Transmembrane	47 – 71	25	1 By similarity
	Topological domain	72 – 78	7	Cytoplasmic By similarity
	Transmembrane	79 – 107	29	2 By similarity
	Topological domain	108 – 121	14	Extracellular By similarity
	Transmembrane	122 – 140	19	3 By similarity
	Topological domain	141 – 159	19	Cytoplasmic By similarity
	Transmembrane	160 – 185	26	4 By similarity
	Topological domain	186 – 201	16	Extracellular By similarity
	Transmembrane	202 – 222	21	5 By similarity
	Topological domain	223 – 256	34	Cytoplasmic By similarity
	Transmembrane	257 – 278	22	6 By similarity
	Topological domain	279 – 294	16	Extracellular By similarity
	Transmembrane	295 – 315	21	7 By similarity
	Topological domain	316 – 382	67	Cytoplasmic By similarity
Amino acid modifications
	Modified residue	353	1	Phosphoserine Potential
	Lipidation	328	1	S-palmitoyl cysteine By similarity
	Glycosylation	30	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	218	1	V → A in AAC53294. Ref.1
	Sequence conflict	244	1	A → G in AAC53294. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O08530-1 [UniParc]. Last modified February 16, 2004. Version 2. Checksum: 2EE4B974E9FBF39C	FASTA	382	42,613
	10 20 30 40 50 60 MVSTSIPEVK ALRSSVSDYG NYDIIVRHYN YTGKLNIGAE KDHGIKLTSV VFILICCFII 70 80 90 100 110 120 LENIFVLLTI WKTKKFHRPM YYFIGNLALS DLLAGVAYTA NLLLSGATTY KLTPAQWFLR 130 140 150 160 170 180 EGSMFVALSA SVFSLLAIAI ERYITMLKMK LHNGSNSSRS FLLISACWVI SLILGGLPSM 190 200 210 220 230 240 GWNCISSLSS CSTVLPLYHK HYILFCTTVF TLLLLSIVIL YCRIYSLVRT RSRRLTFRKN 250 260 270 280 290 300 ISKASRSSEK SLALLKTVII VLSVFIACWA PLFILLLLDV GCKAKTCDIL YKAEYFLVLA 310 320 330 340 350 360 VLNSGTNPII YTLTNKEMRR AFIRIVSCCK CPNGDSAGKF KRPIIPGMEF SRSKSDNSSH 370 380 PQKDDGDNPE TIMSSGNVNS SS

References

[1]	"The mouse gene for the inducible G-protein-coupled receptor edg-1." Liu C.H., Hla T. Genomics 43:15-24(1997) [PubMed: 9226368] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Liver.
[2]	"Comparative analysis of three murine G-protein coupled receptors activated by sphingosine-1-phosphate." Zhang G., Contos J.J.A., Weiner J.A., Fukushima N., Chun J. Gene 227:89-99(1999) [PubMed: 9931453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: 129/SvJ.
[3]	"Role of the sphingosine-1-phosphate receptor EDG-1 in PDGF-induced cell motility." Hobson J.P., Rosenfeldt H.M., Barak L.S., Olivera A., Poulton S., Caron M.G., Milstien S., Spiegel S. Science 291:1800-1803(2001) [PubMed: 11230698] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U40811 Genomic DNA. Translation: AAC53294.1. AF108019 Genomic DNA. Translation: AAD16975.1.
IPI	IPI00308940.
UniGene	Mm.982
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	O08530.
Genome annotation databases
Ensembl	ENSMUSG00000045092. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1096355. S1pr1.
Phylogenomic databases
HOGENOM	O08530.
HOVERGEN	O08530.
Gene expression databases
ArrayExpress	O08530.
Bgee	O08530.
GermOnline	ENSMUSG00000045092. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR000987. EDG1_rcpt. IPR017452. GPCR_Rhodpsn_supfam. IPR004061. S1P_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00642. EDG1RECEPTOR. PR00237. GPCRRHODOPSN. PR01523. S1PRECEPTOR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

S1PR1_MOUSE

Accession

Primary (citable) accession number: O08530
Secondary accession number(s): Q9R235

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 16, 2004
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)