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O08529

- CAN2_MOUSE

UniProt

O08529 - CAN2_MOUSE

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Protein

Calpain-2 catalytic subunit

Gene

Capn2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (By similarity).By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 7 calcium ions.By similarity

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi89 – 891Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi91 – 911Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi96 – 961Calcium 3By similarity
Active sitei105 – 1051By similarity
Metal bindingi175 – 1751Calcium 3By similarity
Metal bindingi229 – 2291Calcium 2By similarity
Metal bindingi230 – 2301Calcium 2By similarity
Active sitei262 – 2621By similarity
Active sitei286 – 2861By similarity
Metal bindingi292 – 2921Calcium 4By similarity
Metal bindingi299 – 2991Calcium 4By similarity
Metal bindingi319 – 3191Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi323 – 3231Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi542 – 5421Calcium 5; via carbonyl oxygenBy similarity
Metal bindingi545 – 5451Calcium 5By similarity
Metal bindingi547 – 5471Calcium 5; via carbonyl oxygenBy similarity
Metal bindingi552 – 5521Calcium 5By similarity
Metal bindingi585 – 5851Calcium 6By similarity
Metal bindingi587 – 5871Calcium 6By similarity
Metal bindingi589 – 5891Calcium 6; via carbonyl oxygenBy similarity
Metal bindingi591 – 5911Calcium 6; via carbonyl oxygenBy similarity
Metal bindingi596 – 5961Calcium 6By similarity
Metal bindingi615 – 6151Calcium 7By similarity
Metal bindingi617 – 6171Calcium 7By similarity
Metal bindingi619 – 6191Calcium 7; via carbonyl oxygenBy similarity
Metal bindingi621 – 6211Calcium 7; via carbonyl oxygenBy similarity
Metal bindingi626 – 6261Calcium 7By similarity
Metal bindingi658 – 6581Calcium 1By similarity
Metal bindingi661 – 6611Calcium 1By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi585 – 596121Add
BLAST
Calcium bindingi615 – 626122Add
BLAST

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
  2. calcium ion binding Source: Ensembl
  3. peptidase activity Source: MGI

GO - Biological processi

  1. blastocyst development Source: MGI
  2. cellular response to amino acid stimulus Source: UniProtKB
  3. myoblast fusion Source: MGI
  4. protein autoprocessing Source: Ensembl
  5. proteolysis Source: UniProtKB
  6. regulation of cytoskeleton organization Source: InterPro
  7. response to hypoxia Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 3474.

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
80 kDa M-calpain subunit
Short name:
CALP80
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:Capn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:88264. Capn2.

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

  1. chromatin Source: MGI
  2. cytoplasm Source: MGI
  3. cytosol Source: BHF-UCL
  4. extracellular vesicular exosome Source: Ensembl
  5. Golgi apparatus Source: Ensembl
  6. membrane raft Source: Ensembl
  7. nucleus Source: MGI
  8. perinuclear endoplasmic reticulum Source: Ensembl
  9. plasma membrane Source: BHF-UCL
  10. pseudopodium Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Propeptidei2 – 1918Anchors to the small subunitSequence AnalysisPRO_0000026491Add
BLAST
Chaini20 – 700681Calpain-2 catalytic subunitPRO_0000026492Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO08529.
PaxDbiO08529.
PRIDEiO08529.

PTM databases

PhosphoSiteiO08529.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiO08529.
CleanExiMM_CAPN2.
ExpressionAtlasiO08529. baseline and differential.
GenevestigatoriO08529.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Protein-protein interaction databases

BioGridi198471. 3 interactions.
IntActiO08529. 4 interactions.
MINTiMINT-1566045.

Structurei

3D structure databases

ProteinModelPortaliO08529.
SMRiO08529. Positions 22-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini572 – 60534EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini602 – 63736EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni345 – 514170Domain IIIAdd
BLAST
Regioni515 – 52915LinkerAdd
BLAST
Regioni530 – 700171Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiO08529.
KOiK03853.
OMAiDTYKKWK.
OrthoDBiEOG7RV9FM.
PhylomeDBiO08529.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08529-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGIAIKLAK DREAAEGLGS HERAIKYLNQ DYETLRNECL EAGALFQDPS
60 70 80 90 100
FPALPSSLGY KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG
110 120 130 140 150
ALGDCWLLAA IASLTLNEEI LARVVPPDQS FQENYAGIFH FQFWQYGEWV
160 170 180 190 200
EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT
210 220 230 240 250
TEGFEDFTGG IAEWYELRKP PPNLFKIIQK ALEKGSLLGC SIDITSAADS
260 270 280 290 300
EAVTYQKLVK GHAYSVTGAE EVESSGSLQK LIRIRNPWGQ VEWTGKWNDN
310 320 330 340 350
CPSWNTVDPE VRANLTERQE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTC
360 370 380 390 400
DSYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED
410 420 430 440 450
GERGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL TGQTNIHLGK
460 470 480 490 500
NFFLTTRARE RSDTFINLRE VLNRFKLPPG EYVLVPSTFE PHKDGDFCIR
510 520 530 540 550
VFSEKKADYQ AVDDEIEANI EEIDANEEDI DDGFRRLFVQ LAGEDAEISA
560 570 580 590 600
FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDEDGSG KLGLKEFYIL
610 620 630 640 650
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKLPC QLHQVIVARF
660 670 680 690 700
ADDELIIDFD NFVRCLVRLE TLFKIFKQLD PENTGTIQLN LASWLSFSVL
Length:700
Mass (Da):79,872
Last modified:January 23, 2007 - v4
Checksum:i682146B290968316
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1941A → T in CAA71227. (PubMed:9339374)Curated
Sequence conflicti212 – 2121A → G in BAA22964. 1 PublicationCurated
Sequence conflicti402 – 4021E → G in CAA71227. (PubMed:9339374)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y10139 mRNA. Translation: CAA71227.1.
D38117 mRNA. Translation: BAA22964.1.
AF015038 mRNA. Translation: AAB94029.1.
BC054726 mRNA. Translation: AAH54726.1.
CCDSiCCDS35813.1.
RefSeqiNP_033924.2. NM_009794.3.
UniGeneiMm.19306.

Genome annotation databases

EnsembliENSMUST00000068505; ENSMUSP00000068895; ENSMUSG00000026509.
GeneIDi12334.
KEGGimmu:12334.
UCSCiuc007dye.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y10139 mRNA. Translation: CAA71227.1 .
D38117 mRNA. Translation: BAA22964.1 .
AF015038 mRNA. Translation: AAB94029.1 .
BC054726 mRNA. Translation: AAH54726.1 .
CCDSi CCDS35813.1.
RefSeqi NP_033924.2. NM_009794.3.
UniGenei Mm.19306.

3D structure databases

ProteinModelPortali O08529.
SMRi O08529. Positions 22-700.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198471. 3 interactions.
IntActi O08529. 4 interactions.
MINTi MINT-1566045.

Protein family/group databases

MEROPSi C02.002.

PTM databases

PhosphoSitei O08529.

Proteomic databases

MaxQBi O08529.
PaxDbi O08529.
PRIDEi O08529.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000068505 ; ENSMUSP00000068895 ; ENSMUSG00000026509 .
GeneIDi 12334.
KEGGi mmu:12334.
UCSCi uc007dye.2. mouse.

Organism-specific databases

CTDi 824.
MGIi MGI:88264. Capn2.

Phylogenomic databases

eggNOGi NOG327523.
GeneTreei ENSGT00760000118971.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi O08529.
KOi K03853.
OMAi DTYKKWK.
OrthoDBi EOG7RV9FM.
PhylomeDBi O08529.
TreeFami TF314748.

Enzyme and pathway databases

BRENDAi 3.4.22.53. 3474.

Miscellaneous databases

ChiTaRSi CAPN2. mouse.
NextBioi 280944.
PROi O08529.
SOURCEi Search...

Gene expression databases

Bgeei O08529.
CleanExi MM_CAPN2.
ExpressionAtlasi O08529. baseline and differential.
Genevestigatori O08529.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution."
    Dear T.N., Matena K., Vingron M., Boehm T.
    Genomics 45:175-184(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. Ozaki Y.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  3. "Cloning of m-calpain 80 kD subunit from the axonal degeneration-resistant WLD(S) mouse mutant."
    Glass J.D., Nash N.R., Dry I., Culver D., Levey A.I., Wesselingh S.
    J. Neurosci. Res. 52:653-660(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: CNS.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiCAN2_MOUSE
AccessioniPrimary (citable) accession number: O08529
Secondary accession number(s): O35518, O54843
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 150 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3