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O08529

- CAN2_MOUSE

UniProt

O08529 - CAN2_MOUSE

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Protein
Calpain-2 catalytic subunit
Gene
Capn2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 7 calcium ions By similarity.

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi89 – 891Calcium 3; via carbonyl oxygen By similarity
Metal bindingi91 – 911Calcium 3; via carbonyl oxygen By similarity
Metal bindingi96 – 961Calcium 3 By similarity
Active sitei105 – 1051 By similarity
Metal bindingi175 – 1751Calcium 3 By similarity
Metal bindingi229 – 2291Calcium 2 By similarity
Metal bindingi230 – 2301Calcium 2 By similarity
Active sitei262 – 2621 By similarity
Active sitei286 – 2861 By similarity
Metal bindingi292 – 2921Calcium 4 By similarity
Metal bindingi299 – 2991Calcium 4 By similarity
Metal bindingi319 – 3191Calcium 4; via carbonyl oxygen By similarity
Metal bindingi323 – 3231Calcium 4; via carbonyl oxygen By similarity
Metal bindingi542 – 5421Calcium 5; via carbonyl oxygen By similarity
Metal bindingi545 – 5451Calcium 5 By similarity
Metal bindingi547 – 5471Calcium 5; via carbonyl oxygen By similarity
Metal bindingi552 – 5521Calcium 5 By similarity
Metal bindingi585 – 5851Calcium 6 By similarity
Metal bindingi587 – 5871Calcium 6 By similarity
Metal bindingi589 – 5891Calcium 6; via carbonyl oxygen By similarity
Metal bindingi591 – 5911Calcium 6; via carbonyl oxygen By similarity
Metal bindingi596 – 5961Calcium 6 By similarity
Metal bindingi615 – 6151Calcium 7 By similarity
Metal bindingi617 – 6171Calcium 7 By similarity
Metal bindingi619 – 6191Calcium 7; via carbonyl oxygen By similarity
Metal bindingi621 – 6211Calcium 7; via carbonyl oxygen By similarity
Metal bindingi626 – 6261Calcium 7 By similarity
Metal bindingi658 – 6581Calcium 1 By similarity
Metal bindingi661 – 6611Calcium 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi585 – 596121
Add
BLAST
Calcium bindingi615 – 626122
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: Ensembl
  2. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
  3. peptidase activity Source: MGI

GO - Biological processi

  1. blastocyst development Source: MGI
  2. cellular response to amino acid stimulus Source: UniProtKB
  3. myoblast fusion Source: MGI
  4. protein autoprocessing Source: Ensembl
  5. proteolysis Source: UniProtKB
  6. response to hypoxia Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 3474.

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
80 kDa M-calpain subunit
Short name:
CALP80
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:Capn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:88264. Capn2.

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding By similarity.

GO - Cellular componenti

  1. chromatin Source: MGI
  2. cytoplasm Source: MGI
  3. cytosol Source: BHF-UCL
  4. nucleus Source: MGI
  5. plasma membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Propeptidei2 – 1918Anchors to the small subunit Reviewed prediction
PRO_0000026491Add
BLAST
Chaini20 – 700681Calpain-2 catalytic subunit
PRO_0000026492Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO08529.
PaxDbiO08529.
PRIDEiO08529.

PTM databases

PhosphoSiteiO08529.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

ArrayExpressiO08529.
BgeeiO08529.
CleanExiMM_CAPN2.
GenevestigatoriO08529.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Protein-protein interaction databases

BioGridi198471. 3 interactions.
IntActiO08529. 4 interactions.
MINTiMINT-1566045.

Structurei

3D structure databases

ProteinModelPortaliO08529.
SMRiO08529. Positions 22-700.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 344300Calpain catalytic
Add
BLAST
Domaini572 – 60534EF-hand 1
Add
BLAST
Domaini602 – 63736EF-hand 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni345 – 514170Domain III
Add
BLAST
Regioni515 – 52915Linker
Add
BLAST
Regioni530 – 700171Domain IV
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.
Contains 2 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
GeneTreeiENSGT00750000117643.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiO08529.
KOiK03853.
OMAiDTYKKWK.
OrthoDBiEOG7RV9FM.
PhylomeDBiO08529.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08529-1 [UniParc]FASTAAdd to Basket

« Hide

MAGIAIKLAK DREAAEGLGS HERAIKYLNQ DYETLRNECL EAGALFQDPS    50
FPALPSSLGY KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG 100
ALGDCWLLAA IASLTLNEEI LARVVPPDQS FQENYAGIFH FQFWQYGEWV 150
EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT 200
TEGFEDFTGG IAEWYELRKP PPNLFKIIQK ALEKGSLLGC SIDITSAADS 250
EAVTYQKLVK GHAYSVTGAE EVESSGSLQK LIRIRNPWGQ VEWTGKWNDN 300
CPSWNTVDPE VRANLTERQE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTC 350
DSYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED 400
GERGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL TGQTNIHLGK 450
NFFLTTRARE RSDTFINLRE VLNRFKLPPG EYVLVPSTFE PHKDGDFCIR 500
VFSEKKADYQ AVDDEIEANI EEIDANEEDI DDGFRRLFVQ LAGEDAEISA 550
FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDEDGSG KLGLKEFYIL 600
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKLPC QLHQVIVARF 650
ADDELIIDFD NFVRCLVRLE TLFKIFKQLD PENTGTIQLN LASWLSFSVL 700
Length:700
Mass (Da):79,872
Last modified:January 23, 2007 - v4
Checksum:i682146B290968316
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1941A → T in CAA71227. 1 Publication
Sequence conflicti212 – 2121A → G in BAA22964. 1 Publication
Sequence conflicti402 – 4021E → G in CAA71227. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y10139 mRNA. Translation: CAA71227.1.
D38117 mRNA. Translation: BAA22964.1.
AF015038 mRNA. Translation: AAB94029.1.
BC054726 mRNA. Translation: AAH54726.1.
CCDSiCCDS35813.1.
RefSeqiNP_033924.2. NM_009794.3.
UniGeneiMm.19306.

Genome annotation databases

EnsembliENSMUST00000068505; ENSMUSP00000068895; ENSMUSG00000026509.
GeneIDi12334.
KEGGimmu:12334.
UCSCiuc007dye.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y10139 mRNA. Translation: CAA71227.1 .
D38117 mRNA. Translation: BAA22964.1 .
AF015038 mRNA. Translation: AAB94029.1 .
BC054726 mRNA. Translation: AAH54726.1 .
CCDSi CCDS35813.1.
RefSeqi NP_033924.2. NM_009794.3.
UniGenei Mm.19306.

3D structure databases

ProteinModelPortali O08529.
SMRi O08529. Positions 22-700.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198471. 3 interactions.
IntActi O08529. 4 interactions.
MINTi MINT-1566045.

Protein family/group databases

MEROPSi C02.002.

PTM databases

PhosphoSitei O08529.

Proteomic databases

MaxQBi O08529.
PaxDbi O08529.
PRIDEi O08529.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000068505 ; ENSMUSP00000068895 ; ENSMUSG00000026509 .
GeneIDi 12334.
KEGGi mmu:12334.
UCSCi uc007dye.2. mouse.

Organism-specific databases

CTDi 824.
MGIi MGI:88264. Capn2.

Phylogenomic databases

eggNOGi NOG327523.
GeneTreei ENSGT00750000117643.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi O08529.
KOi K03853.
OMAi DTYKKWK.
OrthoDBi EOG7RV9FM.
PhylomeDBi O08529.
TreeFami TF314748.

Enzyme and pathway databases

BRENDAi 3.4.22.53. 3474.

Miscellaneous databases

ChiTaRSi CAPN2. mouse.
NextBioi 280944.
PROi O08529.
SOURCEi Search...

Gene expression databases

ArrayExpressi O08529.
Bgeei O08529.
CleanExi MM_CAPN2.
Genevestigatori O08529.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution."
    Dear T.N., Matena K., Vingron M., Boehm T.
    Genomics 45:175-184(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. Ozaki Y.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  3. "Cloning of m-calpain 80 kD subunit from the axonal degeneration-resistant WLD(S) mouse mutant."
    Glass J.D., Nash N.R., Dry I., Culver D., Levey A.I., Wesselingh S.
    J. Neurosci. Res. 52:653-660(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: CNS.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiCAN2_MOUSE
AccessioniPrimary (citable) accession number: O08529
Secondary accession number(s): O35518, O54843
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 148 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi