Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calpain-2 catalytic subunit

Gene

Capn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (By similarity). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (PubMed:22711986).By similarity1 Publication

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 7 Ca2+ ions.By similarity

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi89Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi91Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi96Calcium 3By similarity1
Active sitei105By similarity1
Metal bindingi175Calcium 3By similarity1
Metal bindingi229Calcium 2By similarity1
Metal bindingi230Calcium 2By similarity1
Active sitei262By similarity1
Active sitei286By similarity1
Metal bindingi292Calcium 4By similarity1
Metal bindingi299Calcium 4By similarity1
Metal bindingi319Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi323Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi542Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi545Calcium 5By similarity1
Metal bindingi547Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi552Calcium 5By similarity1
Metal bindingi585Calcium 6By similarity1
Metal bindingi587Calcium 6By similarity1
Metal bindingi589Calcium 6; via carbonyl oxygenBy similarity1
Metal bindingi591Calcium 6; via carbonyl oxygenBy similarity1
Metal bindingi596Calcium 6By similarity1
Metal bindingi615Calcium 7By similarity1
Metal bindingi617Calcium 7By similarity1
Metal bindingi619Calcium 7; via carbonyl oxygenBy similarity1
Metal bindingi621Calcium 7; via carbonyl oxygenBy similarity1
Metal bindingi626Calcium 7By similarity1
Metal bindingi658Calcium 1By similarity1
Metal bindingi661Calcium 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi585 – 5961Add BLAST12
Calcium bindingi615 – 6262Add BLAST12

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
  • calcium ion binding Source: Ensembl
  • peptidase activity Source: MGI

GO - Biological processi

  • blastocyst development Source: MGI
  • cellular response to amino acid stimulus Source: UniProtKB
  • myoblast fusion Source: MGI
  • protein autoprocessing Source: Ensembl
  • proteolysis Source: UniProtKB
  • proteolysis involved in cellular protein catabolic process Source: MGI
  • regulation of cytoskeleton organization Source: InterPro
  • response to hypoxia Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 3474.
ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
80 kDa M-calpain subunit
Short name:
CALP80
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:Capn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88264. Capn2.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

  • chromatin Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: BHF-UCL
  • dendrite Source: Ensembl
  • endoplasmic reticulum Source: MGI
  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
  • Golgi apparatus Source: MGI
  • lysosome Source: MGI
  • membrane raft Source: MGI
  • nucleus Source: MGI
  • perinuclear endoplasmic reticulum Source: MGI
  • plasma membrane Source: BHF-UCL
  • pseudopodium Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
PropeptideiPRO_00000264912 – 19Anchors to the small subunitSequence analysisAdd BLAST18
ChainiPRO_000002649220 – 700Calpain-2 catalytic subunitAdd BLAST681

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO08529.
MaxQBiO08529.
PaxDbiO08529.
PeptideAtlasiO08529.
PRIDEiO08529.

PTM databases

iPTMnetiO08529.
PhosphoSitePlusiO08529.
SwissPalmiO08529.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSMUSG00000026509.
CleanExiMM_CAPN2.
GenevisibleiO08529. MM.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1) (By similarity). Interacts with CPEB3; this leads to cleavage of CPEB3 (PubMed:22711986).By similarity1 Publication

Protein-protein interaction databases

BioGridi198471. 3 interactors.
IntActiO08529. 4 interactors.
MINTiMINT-1566045.
STRINGi10090.ENSMUSP00000068895.

Structurei

3D structure databases

ProteinModelPortaliO08529.
SMRiO08529.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 344Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini572 – 605EF-hand 1PROSITE-ProRule annotationAdd BLAST34
Domaini602 – 637EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni345 – 514Domain IIIAdd BLAST170
Regioni515 – 529LinkerAdd BLAST15
Regioni530 – 700Domain IVAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiO08529.
KOiK03853.
OMAiDTYKKWK.
OrthoDBiEOG091G049E.
PhylomeDBiO08529.
TreeFamiTF314748.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIAIKLAK DREAAEGLGS HERAIKYLNQ DYETLRNECL EAGALFQDPS
60 70 80 90 100
FPALPSSLGY KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG
110 120 130 140 150
ALGDCWLLAA IASLTLNEEI LARVVPPDQS FQENYAGIFH FQFWQYGEWV
160 170 180 190 200
EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT
210 220 230 240 250
TEGFEDFTGG IAEWYELRKP PPNLFKIIQK ALEKGSLLGC SIDITSAADS
260 270 280 290 300
EAVTYQKLVK GHAYSVTGAE EVESSGSLQK LIRIRNPWGQ VEWTGKWNDN
310 320 330 340 350
CPSWNTVDPE VRANLTERQE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTC
360 370 380 390 400
DSYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED
410 420 430 440 450
GERGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL TGQTNIHLGK
460 470 480 490 500
NFFLTTRARE RSDTFINLRE VLNRFKLPPG EYVLVPSTFE PHKDGDFCIR
510 520 530 540 550
VFSEKKADYQ AVDDEIEANI EEIDANEEDI DDGFRRLFVQ LAGEDAEISA
560 570 580 590 600
FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDEDGSG KLGLKEFYIL
610 620 630 640 650
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKLPC QLHQVIVARF
660 670 680 690 700
ADDELIIDFD NFVRCLVRLE TLFKIFKQLD PENTGTIQLN LASWLSFSVL
Length:700
Mass (Da):79,872
Last modified:January 23, 2007 - v4
Checksum:i682146B290968316
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194A → T in CAA71227 (PubMed:9339374).Curated1
Sequence conflicti212A → G in BAA22964 (Ref. 2) Curated1
Sequence conflicti402E → G in CAA71227 (PubMed:9339374).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10139 mRNA. Translation: CAA71227.1.
D38117 mRNA. Translation: BAA22964.1.
AF015038 mRNA. Translation: AAB94029.1.
BC054726 mRNA. Translation: AAH54726.1.
CCDSiCCDS35813.1.
RefSeqiNP_033924.2. NM_009794.3.
UniGeneiMm.19306.
Mm.474138.

Genome annotation databases

EnsembliENSMUST00000068505; ENSMUSP00000068895; ENSMUSG00000026509.
GeneIDi12334.
KEGGimmu:12334.
UCSCiuc007dye.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10139 mRNA. Translation: CAA71227.1.
D38117 mRNA. Translation: BAA22964.1.
AF015038 mRNA. Translation: AAB94029.1.
BC054726 mRNA. Translation: AAH54726.1.
CCDSiCCDS35813.1.
RefSeqiNP_033924.2. NM_009794.3.
UniGeneiMm.19306.
Mm.474138.

3D structure databases

ProteinModelPortaliO08529.
SMRiO08529.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198471. 3 interactors.
IntActiO08529. 4 interactors.
MINTiMINT-1566045.
STRINGi10090.ENSMUSP00000068895.

Protein family/group databases

MEROPSiC02.002.

PTM databases

iPTMnetiO08529.
PhosphoSitePlusiO08529.
SwissPalmiO08529.

Proteomic databases

EPDiO08529.
MaxQBiO08529.
PaxDbiO08529.
PeptideAtlasiO08529.
PRIDEiO08529.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068505; ENSMUSP00000068895; ENSMUSG00000026509.
GeneIDi12334.
KEGGimmu:12334.
UCSCiuc007dye.2. mouse.

Organism-specific databases

CTDi824.
MGIiMGI:88264. Capn2.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiO08529.
KOiK03853.
OMAiDTYKKWK.
OrthoDBiEOG091G049E.
PhylomeDBiO08529.
TreeFamiTF314748.

Enzyme and pathway databases

BRENDAi3.4.22.53. 3474.
ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.

Miscellaneous databases

ChiTaRSiCapn2. mouse.
PROiO08529.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026509.
CleanExiMM_CAPN2.
GenevisibleiO08529. MM.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN2_MOUSE
AccessioniPrimary (citable) accession number: O08529
Secondary accession number(s): O35518, O54843
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 170 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.