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O08529

- CAN2_MOUSE

UniProt

O08529 - CAN2_MOUSE

Protein

Calpain-2 catalytic subunit

Gene

Capn2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.By similarity

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 7 calcium ions.By similarity

    Enzyme regulationi

    Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi89 – 891Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi91 – 911Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi96 – 961Calcium 3By similarity
    Active sitei105 – 1051By similarity
    Metal bindingi175 – 1751Calcium 3By similarity
    Metal bindingi229 – 2291Calcium 2By similarity
    Metal bindingi230 – 2301Calcium 2By similarity
    Active sitei262 – 2621By similarity
    Active sitei286 – 2861By similarity
    Metal bindingi292 – 2921Calcium 4By similarity
    Metal bindingi299 – 2991Calcium 4By similarity
    Metal bindingi319 – 3191Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi323 – 3231Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi542 – 5421Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi545 – 5451Calcium 5By similarity
    Metal bindingi547 – 5471Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi552 – 5521Calcium 5By similarity
    Metal bindingi585 – 5851Calcium 6By similarity
    Metal bindingi587 – 5871Calcium 6By similarity
    Metal bindingi589 – 5891Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi591 – 5911Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi596 – 5961Calcium 6By similarity
    Metal bindingi615 – 6151Calcium 7By similarity
    Metal bindingi617 – 6171Calcium 7By similarity
    Metal bindingi619 – 6191Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi621 – 6211Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi626 – 6261Calcium 7By similarity
    Metal bindingi658 – 6581Calcium 1By similarity
    Metal bindingi661 – 6611Calcium 1By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi585 – 596121Add
    BLAST
    Calcium bindingi615 – 626122Add
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
    2. calcium ion binding Source: Ensembl
    3. peptidase activity Source: MGI

    GO - Biological processi

    1. blastocyst development Source: MGI
    2. cellular response to amino acid stimulus Source: UniProtKB
    3. myoblast fusion Source: MGI
    4. protein autoprocessing Source: Ensembl
    5. proteolysis Source: UniProtKB
    6. response to hypoxia Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.4.22.53. 3474.

    Protein family/group databases

    MEROPSiC02.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-2 catalytic subunit (EC:3.4.22.53)
    Alternative name(s):
    80 kDa M-calpain subunit
    Short name:
    CALP80
    Calcium-activated neutral proteinase 2
    Short name:
    CANP 2
    Calpain M-type
    Calpain-2 large subunit
    Millimolar-calpain
    Short name:
    M-calpain
    Gene namesi
    Name:Capn2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:88264. Capn2.

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

    GO - Cellular componenti

    1. chromatin Source: MGI
    2. cytoplasm Source: MGI
    3. cytosol Source: BHF-UCL
    4. nucleus Source: MGI
    5. plasma membrane Source: BHF-UCL

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Propeptidei2 – 1918Anchors to the small subunitSequence AnalysisPRO_0000026491Add
    BLAST
    Chaini20 – 700681Calpain-2 catalytic subunitPRO_0000026492Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO08529.
    PaxDbiO08529.
    PRIDEiO08529.

    PTM databases

    PhosphoSiteiO08529.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    ArrayExpressiO08529.
    BgeeiO08529.
    CleanExiMM_CAPN2.
    GenevestigatoriO08529.

    Interactioni

    Subunit structurei

    Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

    Protein-protein interaction databases

    BioGridi198471. 3 interactions.
    IntActiO08529. 4 interactions.
    MINTiMINT-1566045.

    Structurei

    3D structure databases

    ProteinModelPortaliO08529.
    SMRiO08529. Positions 22-700.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini572 – 60534EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini602 – 63736EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni345 – 514170Domain IIIAdd
    BLAST
    Regioni515 – 52915LinkerAdd
    BLAST
    Regioni530 – 700171Domain IVAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG327523.
    GeneTreeiENSGT00750000117643.
    HOGENOMiHOG000232035.
    HOVERGENiHBG012645.
    InParanoidiO08529.
    KOiK03853.
    OMAiDTYKKWK.
    OrthoDBiEOG7RV9FM.
    PhylomeDBiO08529.
    TreeFamiTF314748.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
    PfamiPF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O08529-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGIAIKLAK DREAAEGLGS HERAIKYLNQ DYETLRNECL EAGALFQDPS    50
    FPALPSSLGY KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG 100
    ALGDCWLLAA IASLTLNEEI LARVVPPDQS FQENYAGIFH FQFWQYGEWV 150
    EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT 200
    TEGFEDFTGG IAEWYELRKP PPNLFKIIQK ALEKGSLLGC SIDITSAADS 250
    EAVTYQKLVK GHAYSVTGAE EVESSGSLQK LIRIRNPWGQ VEWTGKWNDN 300
    CPSWNTVDPE VRANLTERQE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTC 350
    DSYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED 400
    GERGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL TGQTNIHLGK 450
    NFFLTTRARE RSDTFINLRE VLNRFKLPPG EYVLVPSTFE PHKDGDFCIR 500
    VFSEKKADYQ AVDDEIEANI EEIDANEEDI DDGFRRLFVQ LAGEDAEISA 550
    FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDEDGSG KLGLKEFYIL 600
    WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKLPC QLHQVIVARF 650
    ADDELIIDFD NFVRCLVRLE TLFKIFKQLD PENTGTIQLN LASWLSFSVL 700
    Length:700
    Mass (Da):79,872
    Last modified:January 23, 2007 - v4
    Checksum:i682146B290968316
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti194 – 1941A → T in CAA71227. (PubMed:9339374)Curated
    Sequence conflicti212 – 2121A → G in BAA22964. 1 PublicationCurated
    Sequence conflicti402 – 4021E → G in CAA71227. (PubMed:9339374)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y10139 mRNA. Translation: CAA71227.1.
    D38117 mRNA. Translation: BAA22964.1.
    AF015038 mRNA. Translation: AAB94029.1.
    BC054726 mRNA. Translation: AAH54726.1.
    CCDSiCCDS35813.1.
    RefSeqiNP_033924.2. NM_009794.3.
    UniGeneiMm.19306.

    Genome annotation databases

    EnsembliENSMUST00000068505; ENSMUSP00000068895; ENSMUSG00000026509.
    GeneIDi12334.
    KEGGimmu:12334.
    UCSCiuc007dye.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y10139 mRNA. Translation: CAA71227.1 .
    D38117 mRNA. Translation: BAA22964.1 .
    AF015038 mRNA. Translation: AAB94029.1 .
    BC054726 mRNA. Translation: AAH54726.1 .
    CCDSi CCDS35813.1.
    RefSeqi NP_033924.2. NM_009794.3.
    UniGenei Mm.19306.

    3D structure databases

    ProteinModelPortali O08529.
    SMRi O08529. Positions 22-700.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198471. 3 interactions.
    IntActi O08529. 4 interactions.
    MINTi MINT-1566045.

    Protein family/group databases

    MEROPSi C02.002.

    PTM databases

    PhosphoSitei O08529.

    Proteomic databases

    MaxQBi O08529.
    PaxDbi O08529.
    PRIDEi O08529.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000068505 ; ENSMUSP00000068895 ; ENSMUSG00000026509 .
    GeneIDi 12334.
    KEGGi mmu:12334.
    UCSCi uc007dye.2. mouse.

    Organism-specific databases

    CTDi 824.
    MGIi MGI:88264. Capn2.

    Phylogenomic databases

    eggNOGi NOG327523.
    GeneTreei ENSGT00750000117643.
    HOGENOMi HOG000232035.
    HOVERGENi HBG012645.
    InParanoidi O08529.
    KOi K03853.
    OMAi DTYKKWK.
    OrthoDBi EOG7RV9FM.
    PhylomeDBi O08529.
    TreeFami TF314748.

    Enzyme and pathway databases

    BRENDAi 3.4.22.53. 3474.

    Miscellaneous databases

    ChiTaRSi CAPN2. mouse.
    NextBioi 280944.
    PROi O08529.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08529.
    Bgeei O08529.
    CleanExi MM_CAPN2.
    Genevestigatori O08529.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
    Pfami PF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution."
      Dear T.N., Matena K., Vingron M., Boehm T.
      Genomics 45:175-184(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. Ozaki Y.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    3. "Cloning of m-calpain 80 kD subunit from the axonal degeneration-resistant WLD(S) mouse mutant."
      Glass J.D., Nash N.R., Dry I., Culver D., Levey A.I., Wesselingh S.
      J. Neurosci. Res. 52:653-660(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: CNS.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiCAN2_MOUSE
    AccessioniPrimary (citable) accession number: O08529
    Secondary accession number(s): O35518, O54843
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 149 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3