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Protein

Calpain-2 catalytic subunit

Gene

Capn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (By similarity). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (PubMed:22711986).By similarity1 Publication

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 7 Ca2+ ions.By similarity

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi89Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi91Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi96Calcium 3By similarity1
Active sitei105By similarity1
Metal bindingi175Calcium 3By similarity1
Metal bindingi229Calcium 2By similarity1
Metal bindingi230Calcium 2By similarity1
Active sitei262By similarity1
Active sitei286By similarity1
Metal bindingi292Calcium 4By similarity1
Metal bindingi299Calcium 4By similarity1
Metal bindingi319Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi323Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi542Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi545Calcium 5By similarity1
Metal bindingi547Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi552Calcium 5By similarity1
Metal bindingi585Calcium 6By similarity1
Metal bindingi587Calcium 6By similarity1
Metal bindingi589Calcium 6; via carbonyl oxygenBy similarity1
Metal bindingi591Calcium 6; via carbonyl oxygenBy similarity1
Metal bindingi596Calcium 6By similarity1
Metal bindingi615Calcium 7By similarity1
Metal bindingi617Calcium 7By similarity1
Metal bindingi619Calcium 7; via carbonyl oxygenBy similarity1
Metal bindingi621Calcium 7; via carbonyl oxygenBy similarity1
Metal bindingi626Calcium 7By similarity1
Metal bindingi658Calcium 1By similarity1
Metal bindingi661Calcium 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi585 – 5961Add BLAST12
Calcium bindingi615 – 6262Add BLAST12

GO - Molecular functioni

GO - Biological processi

  • blastocyst development Source: MGI
  • cellular response to amino acid stimulus Source: UniProtKB
  • myoblast fusion Source: MGI
  • protein autoprocessing Source: Ensembl
  • proteolysis Source: UniProtKB
  • proteolysis involved in cellular protein catabolic process Source: MGI
  • regulation of cytoskeleton organization Source: InterPro
  • response to hypoxia Source: Ensembl

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53 3474
ReactomeiR-MMU-1474228 Degradation of the extracellular matrix

Protein family/group databases

MEROPSiC02.002

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
80 kDa M-calpain subunit
Short name:
CALP80
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:Capn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88264 Capn2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
PropeptideiPRO_00000264912 – 19Anchors to the small subunitSequence analysisAdd BLAST18
ChainiPRO_000002649220 – 700Calpain-2 catalytic subunitAdd BLAST681

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO08529
MaxQBiO08529
PaxDbiO08529
PeptideAtlasiO08529
PRIDEiO08529

PTM databases

iPTMnetiO08529
PhosphoSitePlusiO08529
SwissPalmiO08529

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSMUSG00000026509
CleanExiMM_CAPN2
GenevisibleiO08529 MM

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1) (By similarity). Interacts with CPEB3; this leads to cleavage of CPEB3 (PubMed:22711986).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1984714 interactors.
IntActiO08529 4 interactors.
MINTiO08529
STRINGi10090.ENSMUSP00000068895

Structurei

3D structure databases

ProteinModelPortaliO08529
SMRiO08529
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 344Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini572 – 605EF-hand 1PROSITE-ProRule annotationAdd BLAST34
Domaini602 – 637EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni345 – 514Domain IIIAdd BLAST170
Regioni515 – 529LinkerAdd BLAST15
Regioni530 – 700Domain IVAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
GeneTreeiENSGT00760000118971
HOGENOMiHOG000232035
HOVERGENiHBG012645
InParanoidiO08529
KOiK03853
OMAiDTYKKWK
OrthoDBiEOG091G049E
PhylomeDBiO08529
TreeFamiTF314748

Family and domain databases

CDDicd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
InterProiView protein in InterPro
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR029539 CAPN2
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PANTHERiPTHR10183:SF268 PTHR10183:SF268, 1 hit
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF13833 EF-hand_8, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF49758 SSF49758, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIAIKLAK DREAAEGLGS HERAIKYLNQ DYETLRNECL EAGALFQDPS
60 70 80 90 100
FPALPSSLGY KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG
110 120 130 140 150
ALGDCWLLAA IASLTLNEEI LARVVPPDQS FQENYAGIFH FQFWQYGEWV
160 170 180 190 200
EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT
210 220 230 240 250
TEGFEDFTGG IAEWYELRKP PPNLFKIIQK ALEKGSLLGC SIDITSAADS
260 270 280 290 300
EAVTYQKLVK GHAYSVTGAE EVESSGSLQK LIRIRNPWGQ VEWTGKWNDN
310 320 330 340 350
CPSWNTVDPE VRANLTERQE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTC
360 370 380 390 400
DSYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED
410 420 430 440 450
GERGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL TGQTNIHLGK
460 470 480 490 500
NFFLTTRARE RSDTFINLRE VLNRFKLPPG EYVLVPSTFE PHKDGDFCIR
510 520 530 540 550
VFSEKKADYQ AVDDEIEANI EEIDANEEDI DDGFRRLFVQ LAGEDAEISA
560 570 580 590 600
FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDEDGSG KLGLKEFYIL
610 620 630 640 650
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKLPC QLHQVIVARF
660 670 680 690 700
ADDELIIDFD NFVRCLVRLE TLFKIFKQLD PENTGTIQLN LASWLSFSVL
Length:700
Mass (Da):79,872
Last modified:January 23, 2007 - v4
Checksum:i682146B290968316
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194A → T in CAA71227 (PubMed:9339374).Curated1
Sequence conflicti212A → G in BAA22964 (Ref. 2) Curated1
Sequence conflicti402E → G in CAA71227 (PubMed:9339374).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10139 mRNA Translation: CAA71227.1
D38117 mRNA Translation: BAA22964.1
AF015038 mRNA Translation: AAB94029.1
BC054726 mRNA Translation: AAH54726.1
CCDSiCCDS35813.1
RefSeqiNP_033924.2, NM_009794.3
UniGeneiMm.19306
Mm.474138

Genome annotation databases

EnsembliENSMUST00000068505; ENSMUSP00000068895; ENSMUSG00000026509
GeneIDi12334
KEGGimmu:12334
UCSCiuc007dye.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCAN2_MOUSE
AccessioniPrimary (citable) accession number: O08529
Secondary accession number(s): O35518, O54843
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 178 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome