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Protein

Hexokinase-2

Gene

Hk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301ATP 1By similarity
Binding sitei209 – 2091Glucose-6-phosphate 1By similarity
Binding sitei232 – 2321Glucose-6-phosphate 1By similarity
Binding sitei235 – 2351Substrate 1By similarity
Binding sitei260 – 2601Substrate 1By similarity
Binding sitei449 – 4491Glucose-6-phosphate 1By similarity
Binding sitei558 – 5581ATP 2Sequence Analysis
Binding sitei657 – 6571Glucose-6-phosphate 2By similarity
Binding sitei680 – 6801ATP 2By similarity
Binding sitei680 – 6801Glucose-6-phosphate 2By similarity
Binding sitei683 – 6831Substrate 2By similarity
Binding sitei708 – 7081Substrate 2By similarity
Binding sitei897 – 8971Glucose-6-phosphate 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 896ATP 1Sequence Analysis
Nucleotide bindingi425 – 4262ATP 1By similarity
Nucleotide bindingi532 – 5376ATP 2By similarity
Nucleotide bindingi747 – 7482ATP 2By similarity
Nucleotide bindingi784 – 7885ATP 2By similarity
Nucleotide bindingi863 – 8675ATP 2By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. fructokinase activity Source: GO_Central
  3. glucokinase activity Source: GO_Central
  4. glucose binding Source: Ensembl
  5. hexokinase activity Source: MGI
  6. mannokinase activity Source: GO_Central

GO - Biological processi

  1. apoptotic mitochondrial changes Source: MGI
  2. carbohydrate phosphorylation Source: MGI
  3. cellular glucose homeostasis Source: GO_Central
  4. glucose 6-phosphate metabolic process Source: GOC
  5. glucose metabolic process Source: MGI
  6. glycolytic process Source: GO_Central
  7. lactation Source: Ensembl
  8. regulation of glucose import Source: MGI
  9. response to ischemia Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_297294. Glucose transport.
SABIO-RKO08528.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type II
Short name:
HK II
Gene namesi
Name:Hk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1315197. Hk2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: GO_Central
  2. membrane Source: MGI
  3. mitochondrial outer membrane Source: MGI
  4. mitochondrion Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 917917Hexokinase-2PRO_0000197588Add
BLAST

Proteomic databases

MaxQBiO08528.
PaxDbiO08528.
PRIDEiO08528.

PTM databases

PhosphoSiteiO08528.

Expressioni

Gene expression databases

BgeeiO08528.
CleanExiMM_HK2.
ExpressionAtlasiO08528. baseline and differential.
GenevestigatoriO08528.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi200316. 3 interactions.
DIPiDIP-41484N.
IntActiO08528. 1 interaction.
MINTiMINT-262872.
STRINGi10090.ENSMUSP00000000642.

Structurei

3D structure databases

ProteinModelPortaliO08528.
SMRiO08528. Positions 17-913.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 458443Hexokinase 1PROSITE-ProRule annotationAdd
BLAST
Domaini464 – 906443Hexokinase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1212HydrophobicAdd
BLAST
Regioni13 – 475463RegulatoryAdd
BLAST
Regioni73 – 207135Hexokinase small subdomain 1PROSITE-ProRule annotationAdd
BLAST
Regioni84 – 885Glucose-6-phosphate 1 bindingBy similarity
Regioni155 – 1562Substrate 1 bindingBy similarity
Regioni172 – 1732Substrate 1 bindingBy similarity
Regioni208 – 447240Hexokinase large subdomain 1PROSITE-ProRule annotationAdd
BLAST
Regioni208 – 2092Substrate 1 bindingBy similarity
Regioni291 – 2944Substrate 1 bindingBy similarity
Regioni413 – 4153Glucose-6-phosphate 1 bindingBy similarity
Regioni476 – 917442CatalyticAdd
BLAST
Regioni521 – 655135Hexokinase small subdomain 2PROSITE-ProRule annotationAdd
BLAST
Regioni532 – 5365Glucose-6-phosphate 2 bindingBy similarity
Regioni603 – 6042Substrate 2 bindingBy similarity
Regioni620 – 6212Substrate 2 bindingBy similarity
Regioni656 – 895240Hexokinase large subdomain 2PROSITE-ProRule annotationAdd
BLAST
Regioni656 – 6572Substrate 2 bindingBy similarity
Regioni739 – 7424Substrate 2 bindingBy similarity
Regioni861 – 8633Glucose-6-phosphate 2 bindingBy similarity

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 2 hexokinase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiO08528.
KOiK00844.
OMAiHHLGLES.
OrthoDBiEOG7S21X5.
PhylomeDBiO08528.
TreeFamiTF314238.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIASHMIACL FTELNQNQVQ KVDQYLYHMR LSDETLLEIS RRFRKEMEKG
60 70 80 90 100
LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLRVRVTDN
110 120 130 140 150
GLQRVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKEKKLPL
160 170 180 190 200
GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVDLIR KAIQRRGDFD
210 220 230 240 250
IDIVAVVNDT VGTMMTCGYD DQNCEIGLIV GTGSNACYME EMRHIDMVEG
260 270 280 290 300
DEGRMCINME WGAFGDDGTL NDIRTEFDRE IDMGSLNPGK QLFEKMISGM
310 320 330 340 350
YMGELVRLIL VKMAKAELLF QGKLSPELLT TGSFETKDVS DIEDDKDGIQ
360 370 380 390 400
KAYQILVRLG LSPLQEDCVA THRICQIVST RSASLCAATL AAVLWRIKEN
410 420 430 440 450
KGEERLRSTI GVDGSVYKKH PHFAKRLHKA VRRLVPDCDV RFLRSEDGSG
460 470 480 490 500
KGAAMVTAVA YRLADQHRAR QKTLESLKLS HEQLLEVKRR MKVEMEQGLS
510 520 530 540 550
KETHEAAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKR
560 570 580 590 600
RGVEMHNKIY SIPQEVMHGT GEELFDHIVQ CIADFLEYMG MKGVSLPLGF
610 620 630 640 650
TFSFPCQQNS LDQSILLKWT KGFKASGCEG EDVVTLLKEA IRRREEFDLD
660 670 680 690 700
VVAVVNDTVG TMMTCGYEDP HCEVGLIVGT GSNACYMEEM RNVELVDGEE
710 720 730 740 750
GRMCVNMEWG AFGDNGCLDD LRTVFDVAVD ELSLNPGKQR FEKMISGMYL
760 770 780 790 800
GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV
810 820 830 840 850
RAILRHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDKIRENRG
860 870 880 890 900
LDNLKVTVGV DGTLYKLHPH FAKVMHETVR DLAPKCDVSF LESEDGSGKG
910
AALITAVACR IREAGQR
Length:917
Mass (Da):102,535
Last modified:July 1, 1997 - v1
Checksum:i44293E7A92D724B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11666, Y11667, Y11668 Genomic DNA. Translation: CAA72366.1.
AJ238540 mRNA. Translation: CAB72257.1.
BC054472 mRNA. Translation: AAH54472.1.
CCDSiCCDS20263.1.
RefSeqiNP_038848.1. NM_013820.3.
UniGeneiMm.255848.
Mm.469167.

Genome annotation databases

EnsembliENSMUST00000000642; ENSMUSP00000000642; ENSMUSG00000000628.
GeneIDi15277.
KEGGimmu:15277.
UCSCiuc009cll.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11666, Y11667, Y11668 Genomic DNA. Translation: CAA72366.1.
AJ238540 mRNA. Translation: CAB72257.1.
BC054472 mRNA. Translation: AAH54472.1.
CCDSiCCDS20263.1.
RefSeqiNP_038848.1. NM_013820.3.
UniGeneiMm.255848.
Mm.469167.

3D structure databases

ProteinModelPortaliO08528.
SMRiO08528. Positions 17-913.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200316. 3 interactions.
DIPiDIP-41484N.
IntActiO08528. 1 interaction.
MINTiMINT-262872.
STRINGi10090.ENSMUSP00000000642.

PTM databases

PhosphoSiteiO08528.

Proteomic databases

MaxQBiO08528.
PaxDbiO08528.
PRIDEiO08528.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000642; ENSMUSP00000000642; ENSMUSG00000000628.
GeneIDi15277.
KEGGimmu:15277.
UCSCiuc009cll.2. mouse.

Organism-specific databases

CTDi3099.
MGIiMGI:1315197. Hk2.

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiO08528.
KOiK00844.
OMAiHHLGLES.
OrthoDBiEOG7S21X5.
PhylomeDBiO08528.
TreeFamiTF314238.

Enzyme and pathway databases

UniPathwayiUPA00242.
ReactomeiREACT_297294. Glucose transport.
SABIO-RKO08528.

Miscellaneous databases

ChiTaRSiHk2. mouse.
NextBioi287909.
PROiO08528.
SOURCEiSearch...

Gene expression databases

BgeeiO08528.
CleanExiMM_HK2.
ExpressionAtlasiO08528. baseline and differential.
GenevestigatoriO08528.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse hexokinase II gene: structure, cDNA, promoter analysis, and expression pattern."
    Heikkinen S., Suppola S., Malkki M., Deeb S.S., Janne J., Laakso M.
    Mamm. Genome 11:91-96(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiHXK2_MOUSE
AccessioniPrimary (citable) accession number: O08528
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: April 1, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.