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O08528

- HXK2_MOUSE

UniProt

O08528 - HXK2_MOUSE

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Protein

Hexokinase-2

Gene
Hk2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301ATP 1 By similarity
Binding sitei209 – 2091Glucose-6-phosphate 1 By similarity
Binding sitei232 – 2321Glucose-6-phosphate 1 By similarity
Binding sitei235 – 2351Substrate 1 By similarity
Binding sitei260 – 2601Substrate 1 By similarity
Binding sitei449 – 4491Glucose-6-phosphate 1 By similarity
Binding sitei558 – 5581ATP 2 Reviewed prediction
Binding sitei657 – 6571Glucose-6-phosphate 2 By similarity
Binding sitei680 – 6801ATP 2 By similarity
Binding sitei680 – 6801Glucose-6-phosphate 2 By similarity
Binding sitei683 – 6831Substrate 2 By similarity
Binding sitei708 – 7081Substrate 2 By similarity
Binding sitei897 – 8971Glucose-6-phosphate 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 896ATP 1 Reviewed prediction
Nucleotide bindingi425 – 4262ATP 1 By similarity
Nucleotide bindingi532 – 5376ATP 2 By similarity
Nucleotide bindingi747 – 7482ATP 2 By similarity
Nucleotide bindingi784 – 7885ATP 2 By similarity
Nucleotide bindingi863 – 8675ATP 2 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. fructokinase activity Source: RefGenome
  3. glucokinase activity Source: RefGenome
  4. glucose binding Source: Ensembl
  5. hexokinase activity Source: MGI
  6. mannokinase activity Source: RefGenome

GO - Biological processi

  1. apoptotic mitochondrial changes Source: MGI
  2. carbohydrate phosphorylation Source: MGI
  3. cellular glucose homeostasis Source: RefGenome
  4. glucose 6-phosphate metabolic process Source: GOC
  5. glucose metabolic process Source: MGI
  6. glycolytic process Source: RefGenome
  7. lactation Source: Ensembl
  8. regulation of glucose import Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKO08528.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type II
Short name:
HK II
Gene namesi
Name:Hk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1315197. Hk2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RefGenome
  2. mitochondrial outer membrane Source: Ensembl
  3. mitochondrion Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 917917Hexokinase-2PRO_0000197588Add
BLAST

Proteomic databases

MaxQBiO08528.
PaxDbiO08528.
PRIDEiO08528.

PTM databases

PhosphoSiteiO08528.

Expressioni

Gene expression databases

ArrayExpressiO08528.
BgeeiO08528.
CleanExiMM_HK2.
GenevestigatoriO08528.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

BioGridi200316. 3 interactions.
DIPiDIP-41484N.
IntActiO08528. 1 interaction.
MINTiMINT-262872.
STRINGi10090.ENSMUSP00000000642.

Structurei

3D structure databases

ProteinModelPortaliO08528.
SMRiO08528. Positions 17-913.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 221205Hexokinase type-1 1Add
BLAST
Domaini223 – 462240Hexokinase type-2 1Add
BLAST
Domaini465 – 669205Hexokinase type-1 2Add
BLAST
Domaini671 – 910240Hexokinase type-2 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1212HydrophobicAdd
BLAST
Regioni13 – 475463RegulatoryAdd
BLAST
Regioni84 – 885Glucose-6-phosphate 1 binding By similarity
Regioni155 – 1562Substrate 1 binding By similarity
Regioni172 – 1732Substrate 1 binding By similarity
Regioni208 – 2092Substrate 1 binding By similarity
Regioni291 – 2944Substrate 1 binding By similarity
Regioni413 – 4153Glucose-6-phosphate 1 binding By similarity
Regioni476 – 917442CatalyticAdd
BLAST
Regioni532 – 5365Glucose-6-phosphate 2 binding By similarity
Regioni603 – 6042Substrate 2 binding By similarity
Regioni620 – 6212Substrate 2 binding By similarity
Regioni656 – 6572Substrate 2 binding By similarity
Regioni739 – 7424Substrate 2 binding By similarity
Regioni861 – 8633Glucose-6-phosphate 2 binding By similarity

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiO08528.
KOiK00844.
OMAiHHLGLES.
OrthoDBiEOG7S21X5.
PhylomeDBiO08528.
TreeFamiTF314238.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASES. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08528-1 [UniParc]FASTAAdd to Basket

« Hide

MIASHMIACL FTELNQNQVQ KVDQYLYHMR LSDETLLEIS RRFRKEMEKG    50
LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLRVRVTDN 100
GLQRVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKEKKLPL 150
GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVDLIR KAIQRRGDFD 200
IDIVAVVNDT VGTMMTCGYD DQNCEIGLIV GTGSNACYME EMRHIDMVEG 250
DEGRMCINME WGAFGDDGTL NDIRTEFDRE IDMGSLNPGK QLFEKMISGM 300
YMGELVRLIL VKMAKAELLF QGKLSPELLT TGSFETKDVS DIEDDKDGIQ 350
KAYQILVRLG LSPLQEDCVA THRICQIVST RSASLCAATL AAVLWRIKEN 400
KGEERLRSTI GVDGSVYKKH PHFAKRLHKA VRRLVPDCDV RFLRSEDGSG 450
KGAAMVTAVA YRLADQHRAR QKTLESLKLS HEQLLEVKRR MKVEMEQGLS 500
KETHEAAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKR 550
RGVEMHNKIY SIPQEVMHGT GEELFDHIVQ CIADFLEYMG MKGVSLPLGF 600
TFSFPCQQNS LDQSILLKWT KGFKASGCEG EDVVTLLKEA IRRREEFDLD 650
VVAVVNDTVG TMMTCGYEDP HCEVGLIVGT GSNACYMEEM RNVELVDGEE 700
GRMCVNMEWG AFGDNGCLDD LRTVFDVAVD ELSLNPGKQR FEKMISGMYL 750
GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV 800
RAILRHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDKIRENRG 850
LDNLKVTVGV DGTLYKLHPH FAKVMHETVR DLAPKCDVSF LESEDGSGKG 900
AALITAVACR IREAGQR 917
Length:917
Mass (Da):102,535
Last modified:July 1, 1997 - v1
Checksum:i44293E7A92D724B3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11666, Y11667, Y11668 Genomic DNA. Translation: CAA72366.1.
AJ238540 mRNA. Translation: CAB72257.1.
BC054472 mRNA. Translation: AAH54472.1.
CCDSiCCDS20263.1.
RefSeqiNP_038848.1. NM_013820.3.
UniGeneiMm.255848.
Mm.469167.

Genome annotation databases

EnsembliENSMUST00000000642; ENSMUSP00000000642; ENSMUSG00000000628.
GeneIDi15277.
KEGGimmu:15277.
UCSCiuc009cll.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11666 , Y11667 , Y11668 Genomic DNA. Translation: CAA72366.1 .
AJ238540 mRNA. Translation: CAB72257.1 .
BC054472 mRNA. Translation: AAH54472.1 .
CCDSi CCDS20263.1.
RefSeqi NP_038848.1. NM_013820.3.
UniGenei Mm.255848.
Mm.469167.

3D structure databases

ProteinModelPortali O08528.
SMRi O08528. Positions 17-913.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200316. 3 interactions.
DIPi DIP-41484N.
IntActi O08528. 1 interaction.
MINTi MINT-262872.
STRINGi 10090.ENSMUSP00000000642.

PTM databases

PhosphoSitei O08528.

Proteomic databases

MaxQBi O08528.
PaxDbi O08528.
PRIDEi O08528.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000000642 ; ENSMUSP00000000642 ; ENSMUSG00000000628 .
GeneIDi 15277.
KEGGi mmu:15277.
UCSCi uc009cll.2. mouse.

Organism-specific databases

CTDi 3099.
MGIi MGI:1315197. Hk2.

Phylogenomic databases

eggNOGi COG5026.
GeneTreei ENSGT00390000017159.
HOGENOMi HOG000162671.
HOVERGENi HBG005020.
InParanoidi O08528.
KOi K00844.
OMAi HHLGLES.
OrthoDBi EOG7S21X5.
PhylomeDBi O08528.
TreeFami TF314238.

Enzyme and pathway databases

UniPathwayi UPA00242 .
SABIO-RK O08528.

Miscellaneous databases

ChiTaRSi HK2. mouse.
NextBioi 287909.
PROi O08528.
SOURCEi Search...

Gene expression databases

ArrayExpressi O08528.
Bgeei O08528.
CleanExi MM_HK2.
Genevestigatori O08528.

Family and domain databases

InterProi IPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view ]
PANTHERi PTHR19443. PTHR19443. 1 hit.
Pfami PF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view ]
PRINTSi PR00475. HEXOKINASE.
PROSITEi PS00378. HEXOKINASES. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse hexokinase II gene: structure, cDNA, promoter analysis, and expression pattern."
    Heikkinen S., Suppola S., Malkki M., Deeb S.S., Janne J., Laakso M.
    Mamm. Genome 11:91-96(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiHXK2_MOUSE
AccessioniPrimary (citable) accession number: O08528
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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