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O08523 (TECTA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-tectorin
Gene names
Name:Tecta
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length2155 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the major non-collagenous components of the tectorial membrane By similarity. The tectorial membrane is an extracellular matrix of the inner ear that covers the neuroepithelium of the cochlea and contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals.

Subunit structure

May form homomeric filament after self-association or heteromeric filament after association with beta-tectorin. Interacts with CEACAM16 By similarity. Ref.1

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side Probable. Secreted › extracellular space › extracellular matrix. Note: Found in the non-collagenous matrix of the tectorial membrane. Ref.1

Tissue specificity

Cochlea-specific. Ref.1

Domain

Zona pellucida domain may enable to form filaments.

Post-translational modification

3 products of tectorin seem to exist: HMM, MMM and LMM. They may be generated by active processing or the result of proteolysis occurring between intrachain disulfide bonds.

The presence of a hydrophobic C-terminus preceded by a potential cleavage site strongly suggests that tectorins are synthesized as glycosylphosphatidylinositol-linked, membrane-bound precursors. Tectorins are targeted to the apical surface of the inner ear epithelia by the lipid and proteolytically released into the extracellular compartment.

Sequence similarities

Contains 1 NIDO domain.

Contains 3 TIL (trypsin inhibitory-like) domains.

Contains 1 VWFC domain.

Contains 4 VWFD domains.

Contains 1 ZP domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O08523-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O08523-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1659-1663: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.1
Chain25 – 20912067Alpha-tectorin
PRO_0000041737
Propeptide2092 – 215564Removed in mature form Potential
PRO_0000041738

Regions

Domain98 – 252155NIDO
Domain260 – 31455VWFC
Domain321 – 540220VWFD 1
Domain597 – 65054TIL 1
Domain712 – 929218VWFD 2
Domain984 – 103653TIL 2
Domain1099 – 1317219VWFD 3
Domain1372 – 142554TIL 3
Domain1486 – 1694209VWFD 4
Domain1805 – 2059255ZP

Amino acid modifications

Lipidation20911GPI-anchor amidated asparagine Potential
Glycosylation341N-linked (GlcNAc...) Potential
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation2151N-linked (GlcNAc...) Potential
Glycosylation2781N-linked (GlcNAc...) Potential
Glycosylation4551N-linked (GlcNAc...) Potential
Glycosylation5061N-linked (GlcNAc...) Potential
Glycosylation5281N-linked (GlcNAc...) Potential
Glycosylation5601N-linked (GlcNAc...) Potential
Glycosylation6701N-linked (GlcNAc...) Potential
Glycosylation6871N-linked (GlcNAc...) Potential
Glycosylation8131N-linked (GlcNAc...) Potential
Glycosylation8431N-linked (GlcNAc...) Potential
Glycosylation8551N-linked (GlcNAc...) Potential
Glycosylation8981N-linked (GlcNAc...) Potential
Glycosylation9201N-linked (GlcNAc...) Potential
Glycosylation9311N-linked (GlcNAc...) Potential
Glycosylation9491N-linked (GlcNAc...) Potential
Glycosylation10481N-linked (GlcNAc...) Potential
Glycosylation10641N-linked (GlcNAc...) Potential
Glycosylation12351N-linked (GlcNAc...) Potential
Glycosylation13641N-linked (GlcNAc...) Potential
Glycosylation15381N-linked (GlcNAc...) Potential
Glycosylation15651N-linked (GlcNAc...) Potential
Glycosylation17561N-linked (GlcNAc...) Potential
Glycosylation17721N-linked (GlcNAc...) Potential
Glycosylation17941N-linked (GlcNAc...) Potential
Glycosylation18511N-linked (GlcNAc...) Potential
Glycosylation18641N-linked (GlcNAc...) Potential
Glycosylation18801N-linked (GlcNAc...) Potential
Glycosylation19201N-linked (GlcNAc...) Potential
Glycosylation19391N-linked (GlcNAc...) Potential
Disulfide bond1980 ↔ 2040 By similarity

Natural variations

Alternative sequence1659 – 16635Missing in isoform 2.
VSP_010557

Experimental info

Sequence conflict8031S → T in CAA68138. Ref.1
Sequence conflict10241A → R in CAA68138. Ref.1
Sequence conflict10321P → T in CAA68138. Ref.1
Sequence conflict12291V → I in CAA68138. Ref.1
Sequence conflict21481I → V in CAA68138. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 1F93E76E0FFB2C23

FASTA2,155239,433
        10         20         30         40         50         60 
MNYSSLLRIW VSFIFALVRH QAQPRELMYP FWQNDTRTPK VDDGSSSEIK LAIPVFFFGV 

        70         80         90        100        110        120 
PYRTVYVNNN GVVSFNVLVS QFTPESFPLT DGRAFIAPFW ADVHNGIRGE IYYRETMDPA 

       130        140        150        160        170        180 
ILRRATKDIR KYFKDMTTFS ATWVFIVTWE EVTFYGGSST TPVNTFQAVL VSDGSYTFTL 

       190        200        210        220        230        240 
FNYYEINWTT GTASGGDPLT GLGGVMAQAG FNGGNLTNFF SLPGSRTPEI VNIQETTNVN 

       250        260        270        280        290        300 
VPGRWAFKVD GKEIDPANGC TSRGQFLRRG EVFWDDLNCT IKCRCLDFNN EIYCQEASCS 

       310        320        330        340        350        360 
PYEVCEPKGR FFYCSPVETS TCVVFGEPHY HTFDGFLFHF QGSCAYLLAR QCLQTSSLPF 

       370        380        390        400        410        420 
FSVEAKNEHR GGSAVSWVKE LSVEVNGYKI LIPKGSYGKV KVNDLVTSLP VTLELGAVKI 

       430        440        450        460        470        480 
YQSGMSTAVE TDFGLLVTFD GQHYASISIP GSYINSTCGL CGNYNKNPLD DFLRPDGRPA 

       490        500        510        520        530        540 
MSVLDLGESW RVYHADWKCG SGCVDNCTQC DAATEALYFG SDYCGFLNKT DGPLWECGTV 

       550        560        570        580        590        600 
VDATAFVHSC VYDLCSVRDN GTLLCQAIQA YALVCQALGI PIGDWRIQTG CVSTVRCPSF 

       610        620        630        640        650        660 
SHYSVCTSSC PDTCSDLTAS QNCATPCTEG CECNEGFVLS TSQCVPLHKC GCDFDGHYYT 

       670        680        690        700        710        720 
MGEFFWATAN CTVQCLCEEG GDVYCFNKTC RSGEVCAVED GYQGCFPKRE TVCLLSQNQV 

       730        740        750        760        770        780 
LHTFDGAAYA FPSELSYTLL KTCPERPEYL EIDINKKKPD AGPAWLRGVR ILVADQEVKI 

       790        800        810        820        830        840 
GGVGALEVKL NGQDVELPFF HPSGRLEIHR NKNSTTVESK GVVSVQYSDV GLLYIRLSTM 

       850        860        870        880        890        900 
YFNCTGGLCG FFNANASDEF CLPNGKCTDN LAVFLESWTT FEEICNGECG DLLKACNNDS 

       910        920        930        940        950        960 
ELLKFYRSRS RCGIINDPSN SSFLECHGVV NVTAYYRTCL FRLCQSGGNE SELCDSVARY 

       970        980        990       1000       1010       1020 
ASACKNADVE VGPWRTYDFC PLECPENSHF EECMTCTETC ETLALGPICV DSCSEGCQCD 

      1030       1040       1050       1060       1070       1080 
EGYALQGSQC VPRSECGCNF EGHQLATNET FWVDQDCQIF CYCNGTDNSV HCETIPCRDD 

      1090       1100       1110       1120       1130       1140 
EYCMEESGLY YCQPRTDASC IVSGYGHYLT FDGYPFDFQT SCPLILCTTG SRPISDSFPK 

      1150       1160       1170       1180       1190       1200 
FIVTAKNEDR DPSLALWVKQ VDVNVFGYSI VIHRAYKHTV LVNNERLYLP LKLGQGKINI 

      1210       1220       1230       1240       1250       1260 
FSFGFHVVVE TDFGLKVVYD WKTFLSITVP RSMQNGTYGL CGRYNGNPDD DLEMPMGLPA 

      1270       1280       1290       1300       1310       1320 
LSINEFGQSW VKRDTFCQVG CGDRCPSCAK VEGFSKVQQL CSLIPNQNAG FAKCHSKVNP 

      1330       1340       1350       1360       1370       1380 
TFFYKNCLFD SCIDGGAVQT ACSWLQNYAS TCQTQGIAVT GWRNYTSCSV TCPPNSHYES 

      1390       1400       1410       1420       1430       1440 
CVSVCQPRCA AIRLKSDCNH YCVEGCQCDA GYVLNGKSCI LPHNCGCYSD GKYYEPKQLF 

      1450       1460       1470       1480       1490       1500 
WNGDCTRRCR CFRRNLIQCD PRQCKSDEEC ALRSGVRGCF STKTSYCLAA GGGVFRTFDG 

      1510       1520       1530       1540       1550       1560 
AFLRFPANCA FVLSTICQKL PDISFQLIIN FDKWSSPNLT IISPVYFYIN EEQILINDRN 

      1570       1580       1590       1600       1610       1620 
TVKVNGTQVN VPFITGLATK IYSSEGFLVI DTSPDIQIYY NGFNVIKISI SERLQNKVCG 

      1630       1640       1650       1660       1670       1680 
LCGNFNGDMT DDYVTLRGKP VVSSVVLAQS WKTNGMQKRP LAPSCNELQF SQYAATCDNV 

      1690       1700       1710       1720       1730       1740 
HIQAMQGDGY CLKLTDMKGF FQPCYGLLDP LPFYESCYLD GCYNHKKFQL CGSLAAYGEA 

      1750       1760       1770       1780       1790       1800 
CRSFGILSTE WIEKENCSGV VEDPCVGADC PNRTCELDNG GELCGCIEPP PYGNNSHDII 

      1810       1820       1830       1840       1850       1860 
DAEVTCKAAQ MEVSISKCKL FQLGFEREGV RINDRQCSGI EGEDFISFQI NNTKGNCGNI 

      1870       1880       1890       1900       1910       1920 
VQSNGTHIMY KNTIWIESAN NTGNIITRDR TINVEFSCAY ELDIKISLDS VVKPMLSVIN 

      1930       1940       1950       1960       1970       1980 
LTVPTQEGSF TTKMALYKNA SYKHPYRQGE VVLTTRDVLY VGVFVVGADS THLILTLNKC 

      1990       2000       2010       2020       2030       2040 
YATPSRDSND KLRYFIIEGG CQNIKDNTIG IEENGVSLTC RFHVTVFKFI GDYDEVHLHC 

      2050       2060       2070       2080       2090       2100 
AVSLCDSEKY SCKINCPQNS RIATDYSKEH KEQIISVGPI RRKRLDWCED NGGCEQICTS 

      2110       2120       2130       2140       2150 
RVDGPLCSCV TGSLQEDGRS CRASNSSVEL QVWTLLLIMT QISLWHLIYK SGATS

« Hide

Isoform 2 [UniParc].

Checksum: B80D56156F66FA75
Show »

FASTA2,150238,898

References

« Hide 'large scale' references
[1]"The mouse tectorins. Modular matrix proteins of the inner ear homologous to components of the sperm-egg adhesion system."
Legan P.K., Rau A., Keene J.N., Richardson G.P.
J. Biol. Chem. 272:8791-8801(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 25-34, SUBUNIT, SUBCELLULAR LOCATION, POST-TRANSLATIONAL MODIFICATIONS, TISSUE SPECIFICITY.
Strain: CD-1.
Tissue: Cochlea.
[2]"Mutations in the human alpha-tectorin gene cause autosomal dominant non-syndromic hearing impairment."
Verhoeven K., Van Laer L., Kirschhofer K., Legan P.K., Hughes D.C., Schatteman I., Verstreken M., Van Hauwe P., Coucke P., Chen A., Smith R.J.H., Somers T., Offeciers F.E., Van de Heyning P., Richardson G.P., Wachtler F., Kimberling W.J., Willems P.J., Govaerts P.J., Van Camp G.
Nat. Genet. 19:60-62(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X99805 mRNA. Translation: CAA68138.1.
AC156631 Genomic DNA. No translation available.
IPIIPI00114338.
IPI00415299.
PIRT30197.
RefSeqNP_033373.2. NM_009347.2.
UniGeneMm.42209.

3D structure databases

ProteinModelPortalO08523.
ModBaseSearch...

PTM databases

PhosphoSiteO08523.

Proteomic databases

PaxDbO08523.
PRIDEO08523.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000042190; ENSMUSP00000040262; ENSMUSG00000037705.
ENSMUST00000160940; ENSMUSP00000125370; ENSMUSG00000037705.
GeneID21683.
KEGGmmu:21683.
UCSCuc009pau.1. mouse.

Organism-specific databases

CTD7007.
MGIMGI:109575. Tecta.

Phylogenomic databases

eggNOGNOG317991.
GeneTreeENSGT00690000101825.
HOGENOMHOG000168220.
HOVERGENHBG079244.
InParanoidO08523.
OMACVYDLCS.
OrthoDBEOG4GTKC0.

Gene expression databases

BgeeO08523.
CleanExMM_TECTA.
GenevestigatorO08523.
GermOnlineENSMUSG00000037705. Mus musculus.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR003886. Nidogen_extracell_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR006552. VWC_out.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
IPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamPF08742. C8. 4 hits.
PF06119. NIDO. 1 hit.
PF01826. TIL. 3 hits.
PF12714. TILa. 2 hits.
PF00094. VWD. 4 hits.
PF00100. Zona_pellucida. 1 hit.
[Graphical view]
SMARTSM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00539. NIDO. 1 hit.
SM00214. VWC. 1 hit.
SM00215. VWC_out. 1 hit.
SM00216. VWD. 4 hits.
SM00241. ZP. 1 hit.
[Graphical view]
SUPFAMSSF57567. Cysrich_TIL. 3 hits.
PROSITEPS51220. NIDO. 1 hit.
PS50184. VWFC_2. False negative.
PS51233. VWFD. 4 hits.
PS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio300996.
SOURCESearch...

Entry information

Entry nameTECTA_MOUSE
AccessionPrimary (citable) accession number: O08523
Secondary accession number(s): E9QNR3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: July 27, 2011
Last modified: May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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