Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O08523

- TECTA_MOUSE

UniProt

O08523 - TECTA_MOUSE

Protein

Alpha-tectorin

Gene

Tecta

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    One of the major non-collagenous components of the tectorial membrane By similarity. The tectorial membrane is an extracellular matrix of the inner ear that covers the neuroepithelium of the cochlea and contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals.By similarity

    GO - Molecular functioni

    1. extracellular matrix structural constituent Source: MGI
    2. protein binding Source: MGI

    GO - Biological processi

    1. cell-matrix adhesion Source: InterPro
    2. sensory perception of sound Source: MGI

    Keywords - Biological processi

    Hearing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-tectorin
    Gene namesi
    Name:Tecta
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:109575. Tecta.

    Subcellular locationi

    Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication; Extracellular side 1 Publication. Secretedextracellular spaceextracellular matrix 1 Publication
    Note: Found in the non-collagenous matrix of the tectorial membrane.

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell
    3. proteinaceous extracellular matrix Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Extracellular matrix, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Chaini25 – 20912067Alpha-tectorinPRO_0000041737Add
    BLAST
    Propeptidei2092 – 215564Removed in mature formSequence AnalysisPRO_0000041738Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi278 – 2781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi455 – 4551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi506 – 5061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi560 – 5601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi670 – 6701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi687 – 6871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi813 – 8131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi843 – 8431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi855 – 8551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi898 – 8981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi920 – 9201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi931 – 9311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi949 – 9491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1048 – 10481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1064 – 10641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1235 – 12351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1364 – 13641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1538 – 15381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1565 – 15651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1756 – 17561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1772 – 17721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1794 – 17941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1851 – 18511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1864 – 18641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1880 – 18801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1920 – 19201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1939 – 19391N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi1980 ↔ 2040PROSITE-ProRule annotation
    Lipidationi2091 – 20911GPI-anchor amidated asparagineSequence Analysis

    Post-translational modificationi

    3 products of tectorin seem to exist: HMM, MMM and LMM. They may be generated by active processing or the result of proteolysis occurring between intrachain disulfide bonds.
    The presence of a hydrophobic C-terminus preceded by a potential cleavage site strongly suggests that tectorins are synthesized as glycosylphosphatidylinositol-linked, membrane-bound precursors. Tectorins are targeted to the apical surface of the inner ear epithelia by the lipid and proteolytically released into the extracellular compartment.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiO08523.
    PRIDEiO08523.

    PTM databases

    PhosphoSiteiO08523.

    Expressioni

    Tissue specificityi

    Cochlea-specific.1 Publication

    Gene expression databases

    BgeeiO08523.
    CleanExiMM_TECTA.
    GenevestigatoriO08523.

    Interactioni

    Subunit structurei

    May form homomeric filament after self-association or heteromeric filament after association with beta-tectorin. Interacts with CEACAM16 By similarity.By similarity

    Protein-protein interaction databases

    IntActiO08523. 1 interaction.
    MINTiMINT-4137390.

    Structurei

    3D structure databases

    ProteinModelPortaliO08523.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini98 – 252155NIDOPROSITE-ProRule annotationAdd
    BLAST
    Domaini260 – 31455VWFCAdd
    BLAST
    Domaini321 – 540220VWFD 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini597 – 65054TIL 1Add
    BLAST
    Domaini712 – 929218VWFD 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini984 – 103653TIL 2Add
    BLAST
    Domaini1099 – 1317219VWFD 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini1372 – 142554TIL 3Add
    BLAST
    Domaini1486 – 1694209VWFD 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini1805 – 2059255ZPPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Zona pellucida domain may enable to form filaments.

    Sequence similaritiesi

    Contains 1 NIDO domain.PROSITE-ProRule annotation
    Contains 1 VWFC domain.Curated
    Contains 4 VWFD domains.PROSITE-ProRule annotation
    Contains 1 ZP domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG317991.
    GeneTreeiENSGT00730000110288.
    HOGENOMiHOG000168220.
    HOVERGENiHBG079244.
    InParanoidiO08523.
    KOiK18273.
    OMAiYLTFDGY.
    OrthoDBiEOG7SJD3N.
    TreeFamiTF300299.

    Family and domain databases

    InterProiIPR000742. EG-like_dom.
    IPR003886. Nidogen_extracell_dom.
    IPR002919. TIL_dom.
    IPR025615. TILa_dom.
    IPR014853. Unchr_dom_Cys-rich.
    IPR006552. VWC_out.
    IPR001007. VWF_C.
    IPR001846. VWF_type-D.
    IPR001507. ZP_dom.
    IPR017977. ZP_dom_CS.
    [Graphical view]
    PfamiPF08742. C8. 4 hits.
    PF06119. NIDO. 1 hit.
    PF01826. TIL. 3 hits.
    PF12714. TILa. 2 hits.
    PF00094. VWD. 4 hits.
    PF00100. Zona_pellucida. 1 hit.
    [Graphical view]
    SMARTiSM00832. C8. 4 hits.
    SM00181. EGF. 1 hit.
    SM00539. NIDO. 1 hit.
    SM00214. VWC. 1 hit.
    SM00215. VWC_out. 1 hit.
    SM00216. VWD. 4 hits.
    SM00241. ZP. 1 hit.
    [Graphical view]
    SUPFAMiSSF57567. SSF57567. 3 hits.
    PROSITEiPS51220. NIDO. 1 hit.
    PS51233. VWFD. 4 hits.
    PS00682. ZP_1. 1 hit.
    PS51034. ZP_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O08523-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNYSSLLRIW VSFIFALVRH QAQPRELMYP FWQNDTRTPK VDDGSSSEIK     50
    LAIPVFFFGV PYRTVYVNNN GVVSFNVLVS QFTPESFPLT DGRAFIAPFW 100
    ADVHNGIRGE IYYRETMDPA ILRRATKDIR KYFKDMTTFS ATWVFIVTWE 150
    EVTFYGGSST TPVNTFQAVL VSDGSYTFTL FNYYEINWTT GTASGGDPLT 200
    GLGGVMAQAG FNGGNLTNFF SLPGSRTPEI VNIQETTNVN VPGRWAFKVD 250
    GKEIDPANGC TSRGQFLRRG EVFWDDLNCT IKCRCLDFNN EIYCQEASCS 300
    PYEVCEPKGR FFYCSPVETS TCVVFGEPHY HTFDGFLFHF QGSCAYLLAR 350
    QCLQTSSLPF FSVEAKNEHR GGSAVSWVKE LSVEVNGYKI LIPKGSYGKV 400
    KVNDLVTSLP VTLELGAVKI YQSGMSTAVE TDFGLLVTFD GQHYASISIP 450
    GSYINSTCGL CGNYNKNPLD DFLRPDGRPA MSVLDLGESW RVYHADWKCG 500
    SGCVDNCTQC DAATEALYFG SDYCGFLNKT DGPLWECGTV VDATAFVHSC 550
    VYDLCSVRDN GTLLCQAIQA YALVCQALGI PIGDWRIQTG CVSTVRCPSF 600
    SHYSVCTSSC PDTCSDLTAS QNCATPCTEG CECNEGFVLS TSQCVPLHKC 650
    GCDFDGHYYT MGEFFWATAN CTVQCLCEEG GDVYCFNKTC RSGEVCAVED 700
    GYQGCFPKRE TVCLLSQNQV LHTFDGAAYA FPSELSYTLL KTCPERPEYL 750
    EIDINKKKPD AGPAWLRGVR ILVADQEVKI GGVGALEVKL NGQDVELPFF 800
    HPSGRLEIHR NKNSTTVESK GVVSVQYSDV GLLYIRLSTM YFNCTGGLCG 850
    FFNANASDEF CLPNGKCTDN LAVFLESWTT FEEICNGECG DLLKACNNDS 900
    ELLKFYRSRS RCGIINDPSN SSFLECHGVV NVTAYYRTCL FRLCQSGGNE 950
    SELCDSVARY ASACKNADVE VGPWRTYDFC PLECPENSHF EECMTCTETC 1000
    ETLALGPICV DSCSEGCQCD EGYALQGSQC VPRSECGCNF EGHQLATNET 1050
    FWVDQDCQIF CYCNGTDNSV HCETIPCRDD EYCMEESGLY YCQPRTDASC 1100
    IVSGYGHYLT FDGYPFDFQT SCPLILCTTG SRPISDSFPK FIVTAKNEDR 1150
    DPSLALWVKQ VDVNVFGYSI VIHRAYKHTV LVNNERLYLP LKLGQGKINI 1200
    FSFGFHVVVE TDFGLKVVYD WKTFLSITVP RSMQNGTYGL CGRYNGNPDD 1250
    DLEMPMGLPA LSINEFGQSW VKRDTFCQVG CGDRCPSCAK VEGFSKVQQL 1300
    CSLIPNQNAG FAKCHSKVNP TFFYKNCLFD SCIDGGAVQT ACSWLQNYAS 1350
    TCQTQGIAVT GWRNYTSCSV TCPPNSHYES CVSVCQPRCA AIRLKSDCNH 1400
    YCVEGCQCDA GYVLNGKSCI LPHNCGCYSD GKYYEPKQLF WNGDCTRRCR 1450
    CFRRNLIQCD PRQCKSDEEC ALRSGVRGCF STKTSYCLAA GGGVFRTFDG 1500
    AFLRFPANCA FVLSTICQKL PDISFQLIIN FDKWSSPNLT IISPVYFYIN 1550
    EEQILINDRN TVKVNGTQVN VPFITGLATK IYSSEGFLVI DTSPDIQIYY 1600
    NGFNVIKISI SERLQNKVCG LCGNFNGDMT DDYVTLRGKP VVSSVVLAQS 1650
    WKTNGMQKRP LAPSCNELQF SQYAATCDNV HIQAMQGDGY CLKLTDMKGF 1700
    FQPCYGLLDP LPFYESCYLD GCYNHKKFQL CGSLAAYGEA CRSFGILSTE 1750
    WIEKENCSGV VEDPCVGADC PNRTCELDNG GELCGCIEPP PYGNNSHDII 1800
    DAEVTCKAAQ MEVSISKCKL FQLGFEREGV RINDRQCSGI EGEDFISFQI 1850
    NNTKGNCGNI VQSNGTHIMY KNTIWIESAN NTGNIITRDR TINVEFSCAY 1900
    ELDIKISLDS VVKPMLSVIN LTVPTQEGSF TTKMALYKNA SYKHPYRQGE 1950
    VVLTTRDVLY VGVFVVGADS THLILTLNKC YATPSRDSND KLRYFIIEGG 2000
    CQNIKDNTIG IEENGVSLTC RFHVTVFKFI GDYDEVHLHC AVSLCDSEKY 2050
    SCKINCPQNS RIATDYSKEH KEQIISVGPI RRKRLDWCED NGGCEQICTS 2100
    RVDGPLCSCV TGSLQEDGRS CRASNSSVEL QVWTLLLIMT QISLWHLIYK 2150
    SGATS 2155
    Length:2,155
    Mass (Da):239,433
    Last modified:July 27, 2011 - v2
    Checksum:i1F93E76E0FFB2C23
    GO
    Isoform 2 (identifier: O08523-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1659-1663: Missing.

    Show »
    Length:2,150
    Mass (Da):238,898
    Checksum:iB80D56156F66FA75
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti803 – 8031S → T in CAA68138. (PubMed:9079715)Curated
    Sequence conflicti1024 – 10241A → R in CAA68138. (PubMed:9079715)Curated
    Sequence conflicti1032 – 10321P → T in CAA68138. (PubMed:9079715)Curated
    Sequence conflicti1229 – 12291V → I in CAA68138. (PubMed:9079715)Curated
    Sequence conflicti2148 – 21481I → V in CAA68138. (PubMed:9079715)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1659 – 16635Missing in isoform 2. 2 PublicationsVSP_010557

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99805 mRNA. Translation: CAA68138.1.
    AC156631 Genomic DNA. No translation available.
    CCDSiCCDS23087.1. [O08523-1]
    PIRiT30197.
    RefSeqiNP_033373.2. NM_009347.2. [O08523-1]
    XP_006510211.1. XM_006510148.1. [O08523-1]
    XP_006510212.1. XM_006510149.1. [O08523-1]
    XP_006510213.1. XM_006510150.1. [O08523-2]
    UniGeneiMm.42209.

    Genome annotation databases

    EnsembliENSMUST00000042190; ENSMUSP00000040262; ENSMUSG00000037705. [O08523-1]
    ENSMUST00000160940; ENSMUSP00000125370; ENSMUSG00000037705. [O08523-2]
    GeneIDi21683.
    KEGGimmu:21683.
    UCSCiuc009pau.1. mouse. [O08523-1]
    uc012grn.1. mouse. [O08523-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99805 mRNA. Translation: CAA68138.1 .
    AC156631 Genomic DNA. No translation available.
    CCDSi CCDS23087.1. [O08523-1 ]
    PIRi T30197.
    RefSeqi NP_033373.2. NM_009347.2. [O08523-1 ]
    XP_006510211.1. XM_006510148.1. [O08523-1 ]
    XP_006510212.1. XM_006510149.1. [O08523-1 ]
    XP_006510213.1. XM_006510150.1. [O08523-2 ]
    UniGenei Mm.42209.

    3D structure databases

    ProteinModelPortali O08523.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O08523. 1 interaction.
    MINTi MINT-4137390.

    PTM databases

    PhosphoSitei O08523.

    Proteomic databases

    PaxDbi O08523.
    PRIDEi O08523.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000042190 ; ENSMUSP00000040262 ; ENSMUSG00000037705 . [O08523-1 ]
    ENSMUST00000160940 ; ENSMUSP00000125370 ; ENSMUSG00000037705 . [O08523-2 ]
    GeneIDi 21683.
    KEGGi mmu:21683.
    UCSCi uc009pau.1. mouse. [O08523-1 ]
    uc012grn.1. mouse. [O08523-2 ]

    Organism-specific databases

    CTDi 7007.
    MGIi MGI:109575. Tecta.

    Phylogenomic databases

    eggNOGi NOG317991.
    GeneTreei ENSGT00730000110288.
    HOGENOMi HOG000168220.
    HOVERGENi HBG079244.
    InParanoidi O08523.
    KOi K18273.
    OMAi YLTFDGY.
    OrthoDBi EOG7SJD3N.
    TreeFami TF300299.

    Miscellaneous databases

    NextBioi 300996.
    PROi O08523.
    SOURCEi Search...

    Gene expression databases

    Bgeei O08523.
    CleanExi MM_TECTA.
    Genevestigatori O08523.

    Family and domain databases

    InterProi IPR000742. EG-like_dom.
    IPR003886. Nidogen_extracell_dom.
    IPR002919. TIL_dom.
    IPR025615. TILa_dom.
    IPR014853. Unchr_dom_Cys-rich.
    IPR006552. VWC_out.
    IPR001007. VWF_C.
    IPR001846. VWF_type-D.
    IPR001507. ZP_dom.
    IPR017977. ZP_dom_CS.
    [Graphical view ]
    Pfami PF08742. C8. 4 hits.
    PF06119. NIDO. 1 hit.
    PF01826. TIL. 3 hits.
    PF12714. TILa. 2 hits.
    PF00094. VWD. 4 hits.
    PF00100. Zona_pellucida. 1 hit.
    [Graphical view ]
    SMARTi SM00832. C8. 4 hits.
    SM00181. EGF. 1 hit.
    SM00539. NIDO. 1 hit.
    SM00214. VWC. 1 hit.
    SM00215. VWC_out. 1 hit.
    SM00216. VWD. 4 hits.
    SM00241. ZP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57567. SSF57567. 3 hits.
    PROSITEi PS51220. NIDO. 1 hit.
    PS51233. VWFD. 4 hits.
    PS00682. ZP_1. 1 hit.
    PS51034. ZP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mouse tectorins. Modular matrix proteins of the inner ear homologous to components of the sperm-egg adhesion system."
      Legan P.K., Rau A., Keene J.N., Richardson G.P.
      J. Biol. Chem. 272:8791-8801(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 25-34, SUBUNIT, SUBCELLULAR LOCATION, POST-TRANSLATIONAL MODIFICATIONS, TISSUE SPECIFICITY.
      Strain: CD-1.
      Tissue: Cochlea.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiTECTA_MOUSE
    AccessioniPrimary (citable) accession number: O08523
    Secondary accession number(s): E9QNR3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3