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O08523

- TECTA_MOUSE

UniProt

O08523 - TECTA_MOUSE

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Protein

Alpha-tectorin

Gene

Tecta

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

One of the major non-collagenous components of the tectorial membrane (By similarity). The tectorial membrane is an extracellular matrix of the inner ear that covers the neuroepithelium of the cochlea and contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals.By similarity

GO - Molecular functioni

  1. extracellular matrix structural constituent Source: MGI

GO - Biological processi

  1. cell-matrix adhesion Source: InterPro
  2. sensory perception of sound Source: MGI
Complete GO annotation...

Keywords - Biological processi

Hearing

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-tectorin
Gene namesi
Name:Tecta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:109575. Tecta.

Subcellular locationi

Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication; Extracellular side 1 Publication. Secretedextracellular spaceextracellular matrix 1 Publication
Note: Found in the non-collagenous matrix of the tectorial membrane.

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. plasma membrane Source: UniProtKB-KW
  4. proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 20912067Alpha-tectorinPRO_0000041737Add
BLAST
Propeptidei2092 – 215564Removed in mature formSequence AnalysisPRO_0000041738Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi278 – 2781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi455 – 4551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi506 – 5061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi560 – 5601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi670 – 6701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi687 – 6871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi813 – 8131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi843 – 8431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi855 – 8551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi898 – 8981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi920 – 9201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi931 – 9311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi949 – 9491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1048 – 10481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1064 – 10641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1235 – 12351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1364 – 13641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1538 – 15381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1565 – 15651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1756 – 17561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1772 – 17721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1794 – 17941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1851 – 18511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1864 – 18641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1880 – 18801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1920 – 19201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1939 – 19391N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi1980 ↔ 2040PROSITE-ProRule annotation
Lipidationi2091 – 20911GPI-anchor amidated asparagineSequence Analysis

Post-translational modificationi

3 products of tectorin seem to exist: HMM, MMM and LMM. They may be generated by active processing or the result of proteolysis occurring between intrachain disulfide bonds.
The presence of a hydrophobic C-terminus preceded by a potential cleavage site strongly suggests that tectorins are synthesized as glycosylphosphatidylinositol-linked, membrane-bound precursors. Tectorins are targeted to the apical surface of the inner ear epithelia by the lipid and proteolytically released into the extracellular compartment.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiO08523.
PRIDEiO08523.

PTM databases

PhosphoSiteiO08523.

Expressioni

Tissue specificityi

Cochlea-specific.1 Publication

Gene expression databases

BgeeiO08523.
CleanExiMM_TECTA.
GenevestigatoriO08523.

Interactioni

Subunit structurei

May form homomeric filament after self-association or heteromeric filament after association with beta-tectorin. Interacts with CEACAM16 (By similarity).By similarity

Protein-protein interaction databases

IntActiO08523. 1 interaction.
MINTiMINT-4137390.

Structurei

3D structure databases

ProteinModelPortaliO08523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini98 – 252155NIDOPROSITE-ProRule annotationAdd
BLAST
Domaini260 – 31455VWFCAdd
BLAST
Domaini321 – 540220VWFD 1PROSITE-ProRule annotationAdd
BLAST
Domaini597 – 65054TIL 1Add
BLAST
Domaini712 – 929218VWFD 2PROSITE-ProRule annotationAdd
BLAST
Domaini984 – 103653TIL 2Add
BLAST
Domaini1099 – 1317219VWFD 3PROSITE-ProRule annotationAdd
BLAST
Domaini1372 – 142554TIL 3Add
BLAST
Domaini1486 – 1694209VWFD 4PROSITE-ProRule annotationAdd
BLAST
Domaini1805 – 2059255ZPPROSITE-ProRule annotationAdd
BLAST

Domaini

Zona pellucida domain may enable to form filaments.

Sequence similaritiesi

Contains 1 NIDO domain.PROSITE-ProRule annotation
Contains 1 VWFC domain.Curated
Contains 4 VWFD domains.PROSITE-ProRule annotation
Contains 1 ZP domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG317991.
GeneTreeiENSGT00760000118896.
HOGENOMiHOG000168220.
HOVERGENiHBG079244.
InParanoidiO08523.
KOiK18273.
OMAiYLTFDGY.
OrthoDBiEOG7SJD3N.
TreeFamiTF300299.

Family and domain databases

InterProiIPR000742. EG-like_dom.
IPR003886. Nidogen_extracell_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR006552. VWC_out.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
IPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF08742. C8. 4 hits.
PF06119. NIDO. 1 hit.
PF01826. TIL. 3 hits.
PF12714. TILa. 2 hits.
PF00094. VWD. 4 hits.
PF00100. Zona_pellucida. 1 hit.
[Graphical view]
SMARTiSM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00539. NIDO. 1 hit.
SM00214. VWC. 1 hit.
SM00215. VWC_out. 1 hit.
SM00216. VWD. 4 hits.
SM00241. ZP. 1 hit.
[Graphical view]
SUPFAMiSSF57567. SSF57567. 3 hits.
PROSITEiPS51220. NIDO. 1 hit.
PS51233. VWFD. 4 hits.
PS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O08523-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNYSSLLRIW VSFIFALVRH QAQPRELMYP FWQNDTRTPK VDDGSSSEIK
60 70 80 90 100
LAIPVFFFGV PYRTVYVNNN GVVSFNVLVS QFTPESFPLT DGRAFIAPFW
110 120 130 140 150
ADVHNGIRGE IYYRETMDPA ILRRATKDIR KYFKDMTTFS ATWVFIVTWE
160 170 180 190 200
EVTFYGGSST TPVNTFQAVL VSDGSYTFTL FNYYEINWTT GTASGGDPLT
210 220 230 240 250
GLGGVMAQAG FNGGNLTNFF SLPGSRTPEI VNIQETTNVN VPGRWAFKVD
260 270 280 290 300
GKEIDPANGC TSRGQFLRRG EVFWDDLNCT IKCRCLDFNN EIYCQEASCS
310 320 330 340 350
PYEVCEPKGR FFYCSPVETS TCVVFGEPHY HTFDGFLFHF QGSCAYLLAR
360 370 380 390 400
QCLQTSSLPF FSVEAKNEHR GGSAVSWVKE LSVEVNGYKI LIPKGSYGKV
410 420 430 440 450
KVNDLVTSLP VTLELGAVKI YQSGMSTAVE TDFGLLVTFD GQHYASISIP
460 470 480 490 500
GSYINSTCGL CGNYNKNPLD DFLRPDGRPA MSVLDLGESW RVYHADWKCG
510 520 530 540 550
SGCVDNCTQC DAATEALYFG SDYCGFLNKT DGPLWECGTV VDATAFVHSC
560 570 580 590 600
VYDLCSVRDN GTLLCQAIQA YALVCQALGI PIGDWRIQTG CVSTVRCPSF
610 620 630 640 650
SHYSVCTSSC PDTCSDLTAS QNCATPCTEG CECNEGFVLS TSQCVPLHKC
660 670 680 690 700
GCDFDGHYYT MGEFFWATAN CTVQCLCEEG GDVYCFNKTC RSGEVCAVED
710 720 730 740 750
GYQGCFPKRE TVCLLSQNQV LHTFDGAAYA FPSELSYTLL KTCPERPEYL
760 770 780 790 800
EIDINKKKPD AGPAWLRGVR ILVADQEVKI GGVGALEVKL NGQDVELPFF
810 820 830 840 850
HPSGRLEIHR NKNSTTVESK GVVSVQYSDV GLLYIRLSTM YFNCTGGLCG
860 870 880 890 900
FFNANASDEF CLPNGKCTDN LAVFLESWTT FEEICNGECG DLLKACNNDS
910 920 930 940 950
ELLKFYRSRS RCGIINDPSN SSFLECHGVV NVTAYYRTCL FRLCQSGGNE
960 970 980 990 1000
SELCDSVARY ASACKNADVE VGPWRTYDFC PLECPENSHF EECMTCTETC
1010 1020 1030 1040 1050
ETLALGPICV DSCSEGCQCD EGYALQGSQC VPRSECGCNF EGHQLATNET
1060 1070 1080 1090 1100
FWVDQDCQIF CYCNGTDNSV HCETIPCRDD EYCMEESGLY YCQPRTDASC
1110 1120 1130 1140 1150
IVSGYGHYLT FDGYPFDFQT SCPLILCTTG SRPISDSFPK FIVTAKNEDR
1160 1170 1180 1190 1200
DPSLALWVKQ VDVNVFGYSI VIHRAYKHTV LVNNERLYLP LKLGQGKINI
1210 1220 1230 1240 1250
FSFGFHVVVE TDFGLKVVYD WKTFLSITVP RSMQNGTYGL CGRYNGNPDD
1260 1270 1280 1290 1300
DLEMPMGLPA LSINEFGQSW VKRDTFCQVG CGDRCPSCAK VEGFSKVQQL
1310 1320 1330 1340 1350
CSLIPNQNAG FAKCHSKVNP TFFYKNCLFD SCIDGGAVQT ACSWLQNYAS
1360 1370 1380 1390 1400
TCQTQGIAVT GWRNYTSCSV TCPPNSHYES CVSVCQPRCA AIRLKSDCNH
1410 1420 1430 1440 1450
YCVEGCQCDA GYVLNGKSCI LPHNCGCYSD GKYYEPKQLF WNGDCTRRCR
1460 1470 1480 1490 1500
CFRRNLIQCD PRQCKSDEEC ALRSGVRGCF STKTSYCLAA GGGVFRTFDG
1510 1520 1530 1540 1550
AFLRFPANCA FVLSTICQKL PDISFQLIIN FDKWSSPNLT IISPVYFYIN
1560 1570 1580 1590 1600
EEQILINDRN TVKVNGTQVN VPFITGLATK IYSSEGFLVI DTSPDIQIYY
1610 1620 1630 1640 1650
NGFNVIKISI SERLQNKVCG LCGNFNGDMT DDYVTLRGKP VVSSVVLAQS
1660 1670 1680 1690 1700
WKTNGMQKRP LAPSCNELQF SQYAATCDNV HIQAMQGDGY CLKLTDMKGF
1710 1720 1730 1740 1750
FQPCYGLLDP LPFYESCYLD GCYNHKKFQL CGSLAAYGEA CRSFGILSTE
1760 1770 1780 1790 1800
WIEKENCSGV VEDPCVGADC PNRTCELDNG GELCGCIEPP PYGNNSHDII
1810 1820 1830 1840 1850
DAEVTCKAAQ MEVSISKCKL FQLGFEREGV RINDRQCSGI EGEDFISFQI
1860 1870 1880 1890 1900
NNTKGNCGNI VQSNGTHIMY KNTIWIESAN NTGNIITRDR TINVEFSCAY
1910 1920 1930 1940 1950
ELDIKISLDS VVKPMLSVIN LTVPTQEGSF TTKMALYKNA SYKHPYRQGE
1960 1970 1980 1990 2000
VVLTTRDVLY VGVFVVGADS THLILTLNKC YATPSRDSND KLRYFIIEGG
2010 2020 2030 2040 2050
CQNIKDNTIG IEENGVSLTC RFHVTVFKFI GDYDEVHLHC AVSLCDSEKY
2060 2070 2080 2090 2100
SCKINCPQNS RIATDYSKEH KEQIISVGPI RRKRLDWCED NGGCEQICTS
2110 2120 2130 2140 2150
RVDGPLCSCV TGSLQEDGRS CRASNSSVEL QVWTLLLIMT QISLWHLIYK

SGATS
Length:2,155
Mass (Da):239,433
Last modified:July 27, 2011 - v2
Checksum:i1F93E76E0FFB2C23
GO
Isoform 2 (identifier: O08523-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1659-1663: Missing.

Show »
Length:2,150
Mass (Da):238,898
Checksum:iB80D56156F66FA75
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti803 – 8031S → T in CAA68138. (PubMed:9079715)Curated
Sequence conflicti1024 – 10241A → R in CAA68138. (PubMed:9079715)Curated
Sequence conflicti1032 – 10321P → T in CAA68138. (PubMed:9079715)Curated
Sequence conflicti1229 – 12291V → I in CAA68138. (PubMed:9079715)Curated
Sequence conflicti2148 – 21481I → V in CAA68138. (PubMed:9079715)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1659 – 16635Missing in isoform 2. 2 PublicationsVSP_010557

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99805 mRNA. Translation: CAA68138.1.
AC156631 Genomic DNA. No translation available.
CCDSiCCDS23087.1. [O08523-1]
PIRiT30197.
RefSeqiNP_033373.2. NM_009347.2. [O08523-1]
XP_006510211.1. XM_006510148.1. [O08523-1]
XP_006510212.1. XM_006510149.1. [O08523-1]
XP_006510213.1. XM_006510150.1. [O08523-2]
UniGeneiMm.42209.

Genome annotation databases

EnsembliENSMUST00000042190; ENSMUSP00000040262; ENSMUSG00000037705. [O08523-1]
ENSMUST00000160940; ENSMUSP00000125370; ENSMUSG00000037705. [O08523-2]
GeneIDi21683.
KEGGimmu:21683.
UCSCiuc009pau.1. mouse. [O08523-1]
uc012grn.1. mouse. [O08523-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99805 mRNA. Translation: CAA68138.1 .
AC156631 Genomic DNA. No translation available.
CCDSi CCDS23087.1. [O08523-1 ]
PIRi T30197.
RefSeqi NP_033373.2. NM_009347.2. [O08523-1 ]
XP_006510211.1. XM_006510148.1. [O08523-1 ]
XP_006510212.1. XM_006510149.1. [O08523-1 ]
XP_006510213.1. XM_006510150.1. [O08523-2 ]
UniGenei Mm.42209.

3D structure databases

ProteinModelPortali O08523.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O08523. 1 interaction.
MINTi MINT-4137390.

PTM databases

PhosphoSitei O08523.

Proteomic databases

PaxDbi O08523.
PRIDEi O08523.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000042190 ; ENSMUSP00000040262 ; ENSMUSG00000037705 . [O08523-1 ]
ENSMUST00000160940 ; ENSMUSP00000125370 ; ENSMUSG00000037705 . [O08523-2 ]
GeneIDi 21683.
KEGGi mmu:21683.
UCSCi uc009pau.1. mouse. [O08523-1 ]
uc012grn.1. mouse. [O08523-2 ]

Organism-specific databases

CTDi 7007.
MGIi MGI:109575. Tecta.

Phylogenomic databases

eggNOGi NOG317991.
GeneTreei ENSGT00760000118896.
HOGENOMi HOG000168220.
HOVERGENi HBG079244.
InParanoidi O08523.
KOi K18273.
OMAi YLTFDGY.
OrthoDBi EOG7SJD3N.
TreeFami TF300299.

Miscellaneous databases

NextBioi 300996.
PROi O08523.
SOURCEi Search...

Gene expression databases

Bgeei O08523.
CleanExi MM_TECTA.
Genevestigatori O08523.

Family and domain databases

InterProi IPR000742. EG-like_dom.
IPR003886. Nidogen_extracell_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR006552. VWC_out.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
IPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view ]
Pfami PF08742. C8. 4 hits.
PF06119. NIDO. 1 hit.
PF01826. TIL. 3 hits.
PF12714. TILa. 2 hits.
PF00094. VWD. 4 hits.
PF00100. Zona_pellucida. 1 hit.
[Graphical view ]
SMARTi SM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00539. NIDO. 1 hit.
SM00214. VWC. 1 hit.
SM00215. VWC_out. 1 hit.
SM00216. VWD. 4 hits.
SM00241. ZP. 1 hit.
[Graphical view ]
SUPFAMi SSF57567. SSF57567. 3 hits.
PROSITEi PS51220. NIDO. 1 hit.
PS51233. VWFD. 4 hits.
PS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse tectorins. Modular matrix proteins of the inner ear homologous to components of the sperm-egg adhesion system."
    Legan P.K., Rau A., Keene J.N., Richardson G.P.
    J. Biol. Chem. 272:8791-8801(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 25-34, SUBUNIT, SUBCELLULAR LOCATION, POST-TRANSLATIONAL MODIFICATIONS, TISSUE SPECIFICITY.
    Strain: CD-1.
    Tissue: Cochlea.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiTECTA_MOUSE
AccessioniPrimary (citable) accession number: O08523
Secondary accession number(s): E9QNR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3