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Protein

Alpha-tectorin

Gene

Tecta

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of the major non-collagenous components of the tectorial membrane (By similarity). The tectorial membrane is an extracellular matrix of the inner ear that covers the neuroepithelium of the cochlea and contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals.By similarity

GO - Molecular functioni

  • extracellular matrix structural constituent Source: MGI

GO - Biological processi

  • cell-matrix adhesion Source: InterPro
  • sensory perception of sound Source: MGI

Keywordsi

Biological processHearing

Enzyme and pathway databases

ReactomeiR-MMU-163125. Post-translational modification: synthesis of GPI-anchored proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-tectorin
Gene namesi
Name:Tecta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:109575. Tecta.

Subcellular locationi

  • Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication; Extracellular side 1 Publication
  • Secretedextracellular spaceextracellular matrix 1 Publication

  • Note: Found in the non-collagenous matrix of the tectorial membrane.

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000004173725 – 2091Alpha-tectorinAdd BLAST2067
PropeptideiPRO_00000417382092 – 2155Removed in mature formSequence analysisAdd BLAST64

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi187N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi215N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi278N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi455N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi506N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi528N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi560N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi670N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi687N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi813N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi843N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi855N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi898N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi920N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi931N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi949N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1048N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1064N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1235N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1364N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1538N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1565N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1717 ↔ 1775By similarity
Disulfide bondi1741 ↔ 1784By similarity
Glycosylationi1756N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1772N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1786 ↔ 1818By similarity
Glycosylationi1794N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1806 ↔ 1898By similarity
Disulfide bondi1837 ↔ 1857By similarity
Glycosylationi1851N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1864N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1880N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1920N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1939N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1980 ↔ 2040PROSITE-ProRule annotation
Disulfide bondi2001 ↔ 2056By similarity
Disulfide bondi2045 ↔ 2052By similarity
Lipidationi2091GPI-anchor amidated asparagineSequence analysis1

Post-translational modificationi

3 products of tectorin seem to exist: HMM, MMM and LMM. They may be generated by active processing or the result of proteolysis occurring between intrachain disulfide bonds.
The presence of a hydrophobic C-terminus preceded by a potential cleavage site strongly suggests that tectorins are synthesized as glycosylphosphatidylinositol-linked, membrane-bound precursors. Tectorins are targeted to the apical surface of the inner ear epithelia by the lipid and proteolytically released into the extracellular compartment.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiO08523.
PaxDbiO08523.
PRIDEiO08523.

PTM databases

PhosphoSitePlusiO08523.

Expressioni

Tissue specificityi

Cochlea-specific.1 Publication

Gene expression databases

BgeeiENSMUSG00000037705.
CleanExiMM_TECTA.
GenevisibleiO08523. MM.

Interactioni

Subunit structurei

May form homomeric filament after self-association or heteromeric filament after association with beta-tectorin. Interacts with CEACAM16 (By similarity).By similarity

Protein-protein interaction databases

IntActiO08523. 1 interactor.
MINTiMINT-4137390.
STRINGi10090.ENSMUSP00000040262.

Structurei

3D structure databases

ProteinModelPortaliO08523.
SMRiO08523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 252NIDOPROSITE-ProRule annotationAdd BLAST155
Domaini260 – 314VWFCAdd BLAST55
Domaini321 – 540VWFD 1PROSITE-ProRule annotationAdd BLAST220
Domaini597 – 650TIL 1Add BLAST54
Domaini712 – 929VWFD 2PROSITE-ProRule annotationAdd BLAST218
Domaini984 – 1036TIL 2Add BLAST53
Domaini1099 – 1317VWFD 3PROSITE-ProRule annotationAdd BLAST219
Domaini1372 – 1425TIL 3Add BLAST54
Domaini1486 – 1694VWFD 4PROSITE-ProRule annotationAdd BLAST209
Domaini1805 – 2059ZPPROSITE-ProRule annotationAdd BLAST255

Domaini

Zona pellucida domain may enable to form filaments.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1216. Eukaryota.
KOG4291. Eukaryota.
ENOG410YR2E. LUCA.
GeneTreeiENSGT00760000118896.
HOGENOMiHOG000168220.
HOVERGENiHBG079244.
InParanoidiO08523.
KOiK18273.
OMAiYSCKINC.
OrthoDBiEOG091G002A.
TreeFamiTF300299.

Family and domain databases

InterProiView protein in InterPro
IPR003886. NIDO_dom.
IPR033026. TECTA.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_dom.
IPR001846. VWF_type-D.
IPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
PANTHERiPTHR11339:SF332. PTHR11339:SF332. 1 hit.
PfamiView protein in Pfam
PF08742. C8. 4 hits.
PF06119. NIDO. 1 hit.
PF01826. TIL. 3 hits.
PF12714. TILa. 2 hits.
PF00094. VWD. 4 hits.
PF00100. Zona_pellucida. 1 hit.
SMARTiView protein in SMART
SM00832. C8. 4 hits.
SM00539. NIDO. 1 hit.
SM00215. VWC_out. 3 hits.
SM00216. VWD. 4 hits.
SM00241. ZP. 1 hit.
SUPFAMiSSF57567. SSF57567. 3 hits.
PROSITEiView protein in PROSITE
PS51220. NIDO. 1 hit.
PS51233. VWFD. 4 hits.
PS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O08523-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNYSSLLRIW VSFIFALVRH QAQPRELMYP FWQNDTRTPK VDDGSSSEIK
60 70 80 90 100
LAIPVFFFGV PYRTVYVNNN GVVSFNVLVS QFTPESFPLT DGRAFIAPFW
110 120 130 140 150
ADVHNGIRGE IYYRETMDPA ILRRATKDIR KYFKDMTTFS ATWVFIVTWE
160 170 180 190 200
EVTFYGGSST TPVNTFQAVL VSDGSYTFTL FNYYEINWTT GTASGGDPLT
210 220 230 240 250
GLGGVMAQAG FNGGNLTNFF SLPGSRTPEI VNIQETTNVN VPGRWAFKVD
260 270 280 290 300
GKEIDPANGC TSRGQFLRRG EVFWDDLNCT IKCRCLDFNN EIYCQEASCS
310 320 330 340 350
PYEVCEPKGR FFYCSPVETS TCVVFGEPHY HTFDGFLFHF QGSCAYLLAR
360 370 380 390 400
QCLQTSSLPF FSVEAKNEHR GGSAVSWVKE LSVEVNGYKI LIPKGSYGKV
410 420 430 440 450
KVNDLVTSLP VTLELGAVKI YQSGMSTAVE TDFGLLVTFD GQHYASISIP
460 470 480 490 500
GSYINSTCGL CGNYNKNPLD DFLRPDGRPA MSVLDLGESW RVYHADWKCG
510 520 530 540 550
SGCVDNCTQC DAATEALYFG SDYCGFLNKT DGPLWECGTV VDATAFVHSC
560 570 580 590 600
VYDLCSVRDN GTLLCQAIQA YALVCQALGI PIGDWRIQTG CVSTVRCPSF
610 620 630 640 650
SHYSVCTSSC PDTCSDLTAS QNCATPCTEG CECNEGFVLS TSQCVPLHKC
660 670 680 690 700
GCDFDGHYYT MGEFFWATAN CTVQCLCEEG GDVYCFNKTC RSGEVCAVED
710 720 730 740 750
GYQGCFPKRE TVCLLSQNQV LHTFDGAAYA FPSELSYTLL KTCPERPEYL
760 770 780 790 800
EIDINKKKPD AGPAWLRGVR ILVADQEVKI GGVGALEVKL NGQDVELPFF
810 820 830 840 850
HPSGRLEIHR NKNSTTVESK GVVSVQYSDV GLLYIRLSTM YFNCTGGLCG
860 870 880 890 900
FFNANASDEF CLPNGKCTDN LAVFLESWTT FEEICNGECG DLLKACNNDS
910 920 930 940 950
ELLKFYRSRS RCGIINDPSN SSFLECHGVV NVTAYYRTCL FRLCQSGGNE
960 970 980 990 1000
SELCDSVARY ASACKNADVE VGPWRTYDFC PLECPENSHF EECMTCTETC
1010 1020 1030 1040 1050
ETLALGPICV DSCSEGCQCD EGYALQGSQC VPRSECGCNF EGHQLATNET
1060 1070 1080 1090 1100
FWVDQDCQIF CYCNGTDNSV HCETIPCRDD EYCMEESGLY YCQPRTDASC
1110 1120 1130 1140 1150
IVSGYGHYLT FDGYPFDFQT SCPLILCTTG SRPISDSFPK FIVTAKNEDR
1160 1170 1180 1190 1200
DPSLALWVKQ VDVNVFGYSI VIHRAYKHTV LVNNERLYLP LKLGQGKINI
1210 1220 1230 1240 1250
FSFGFHVVVE TDFGLKVVYD WKTFLSITVP RSMQNGTYGL CGRYNGNPDD
1260 1270 1280 1290 1300
DLEMPMGLPA LSINEFGQSW VKRDTFCQVG CGDRCPSCAK VEGFSKVQQL
1310 1320 1330 1340 1350
CSLIPNQNAG FAKCHSKVNP TFFYKNCLFD SCIDGGAVQT ACSWLQNYAS
1360 1370 1380 1390 1400
TCQTQGIAVT GWRNYTSCSV TCPPNSHYES CVSVCQPRCA AIRLKSDCNH
1410 1420 1430 1440 1450
YCVEGCQCDA GYVLNGKSCI LPHNCGCYSD GKYYEPKQLF WNGDCTRRCR
1460 1470 1480 1490 1500
CFRRNLIQCD PRQCKSDEEC ALRSGVRGCF STKTSYCLAA GGGVFRTFDG
1510 1520 1530 1540 1550
AFLRFPANCA FVLSTICQKL PDISFQLIIN FDKWSSPNLT IISPVYFYIN
1560 1570 1580 1590 1600
EEQILINDRN TVKVNGTQVN VPFITGLATK IYSSEGFLVI DTSPDIQIYY
1610 1620 1630 1640 1650
NGFNVIKISI SERLQNKVCG LCGNFNGDMT DDYVTLRGKP VVSSVVLAQS
1660 1670 1680 1690 1700
WKTNGMQKRP LAPSCNELQF SQYAATCDNV HIQAMQGDGY CLKLTDMKGF
1710 1720 1730 1740 1750
FQPCYGLLDP LPFYESCYLD GCYNHKKFQL CGSLAAYGEA CRSFGILSTE
1760 1770 1780 1790 1800
WIEKENCSGV VEDPCVGADC PNRTCELDNG GELCGCIEPP PYGNNSHDII
1810 1820 1830 1840 1850
DAEVTCKAAQ MEVSISKCKL FQLGFEREGV RINDRQCSGI EGEDFISFQI
1860 1870 1880 1890 1900
NNTKGNCGNI VQSNGTHIMY KNTIWIESAN NTGNIITRDR TINVEFSCAY
1910 1920 1930 1940 1950
ELDIKISLDS VVKPMLSVIN LTVPTQEGSF TTKMALYKNA SYKHPYRQGE
1960 1970 1980 1990 2000
VVLTTRDVLY VGVFVVGADS THLILTLNKC YATPSRDSND KLRYFIIEGG
2010 2020 2030 2040 2050
CQNIKDNTIG IEENGVSLTC RFHVTVFKFI GDYDEVHLHC AVSLCDSEKY
2060 2070 2080 2090 2100
SCKINCPQNS RIATDYSKEH KEQIISVGPI RRKRLDWCED NGGCEQICTS
2110 2120 2130 2140 2150
RVDGPLCSCV TGSLQEDGRS CRASNSSVEL QVWTLLLIMT QISLWHLIYK

SGATS
Length:2,155
Mass (Da):239,433
Last modified:July 27, 2011 - v2
Checksum:i1F93E76E0FFB2C23
GO
Isoform 2 (identifier: O08523-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1659-1663: Missing.

Show »
Length:2,150
Mass (Da):238,898
Checksum:iB80D56156F66FA75
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti803S → T in CAA68138 (PubMed:9079715).Curated1
Sequence conflicti1024A → R in CAA68138 (PubMed:9079715).Curated1
Sequence conflicti1032P → T in CAA68138 (PubMed:9079715).Curated1
Sequence conflicti1229V → I in CAA68138 (PubMed:9079715).Curated1
Sequence conflicti2148I → V in CAA68138 (PubMed:9079715).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0105571659 – 1663Missing in isoform 2. 2 Publications5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99805 mRNA. Translation: CAA68138.1.
AC156631 Genomic DNA. No translation available.
CCDSiCCDS23087.1. [O08523-1]
CCDS85660.1. [O08523-2]
PIRiT30197.
RefSeqiNP_001311477.1. NM_001324548.1. [O08523-2]
NP_033373.2. NM_009347.3. [O08523-1]
XP_017168765.1. XM_017313276.1. [O08523-1]
UniGeneiMm.42209.

Genome annotation databases

EnsembliENSMUST00000042190; ENSMUSP00000040262; ENSMUSG00000037705. [O08523-1]
ENSMUST00000160940; ENSMUSP00000125370; ENSMUSG00000037705. [O08523-2]
GeneIDi21683.
KEGGimmu:21683.
UCSCiuc009pau.1. mouse. [O08523-1]
uc012grn.1. mouse. [O08523-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTECTA_MOUSE
AccessioniPrimary (citable) accession number: O08523
Secondary accession number(s): E9QNR3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: July 27, 2011
Last modified: July 5, 2017
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot