O08467Q5JJ67GLNA_THEKOGlutamine synthetaseGS6.3.1.2Glutamate--ammonia ligaseGlutamine synthetase I alphaGSI alphaglnATK1796Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)Pyrococcus kodakaraensis (strain KOD1)ArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcusCharacterization of recombinant glutamine synthetase from the hyperthermophilic archaeon Pyrococcus sp. strain KOD1.NUCLEOTIDE SEQUENCE [GENOMIC DNA]FUNCTIONCATALYTIC ACTIVITYGENE NAMESUBSTRATE SPECIFICITYBIOPHYSICOCHEMICAL PROPERTIESCOFACTORSUBUNITComplete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes.NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]Carries out the ATP-dependent synthesis of glutamine from ammonium nitrogen and glutamate. Exhibits both L-gamma-glutamylhydroxamate synthetase and gamma-glutamyltransferase activities when using hydroxylamine as substrate; in fact, the enzyme possesses low biosynthetic activity, suggesting that the reaction is biased towards the degradation of glutamine under ammonia-rich conditions. Might play some role in ammonia assimilation under ammonia-starvation conditions. Can also use GTP instead of ATP in the synthetase reaction, but not CTP or UTP.ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine + phosphateATP + hydroxylamine + L-glutamate = ADP + L-glutamine hydroxamate + phosphateMg(2+)Mn(2+)Binds 2 Mg(2+) or Mn(2+) ions per subunit.The activity of this enzyme is not controlled by adenylation.23.5 mM for L-glutamate (at pH 7.8 and 60 degrees Celsius)15.2 mM for hydroxylamine (when a high concentration is used, at pH 7.8 and 60 degrees Celsius)1.6 mM for hydroxylamine (when a low concentration is used, at pH 7.8 and 60 degrees Celsius)28 mM for ATP (at pH 7.8 and 60 degrees Celsius)5 mM for L-glutamine (at pH 7.2 and 60 degrees Celsius)6.3 mM for ADP (at pH 7.2 and 60 degrees Celsius)kcat is 2190 min(-1) towards L-glutamate in the synthetase reaction and 3900 min(-1) towards L-glutamine in the transferase reaction.Optimum pH is 7.8 for the synthetase reaction and pH 7.2 for the transferase reaction.Optimum temperature is 60 degrees Celsius for both transferase and synthetase activities.Oligomer of 12 subunits arranged in the form of two hexagons.CytoplasmBelongs to the glutamine synthetase family.ATP-bindingCytoplasmLigaseMagnesiumManganeseMetal-bindingNucleotide-bindingReference proteomeMg(2+)Mg(2+)ATPMg(2+)Mg(2+)L-glutamateMg(2+)ATPATPL-glutamateL-glutamateATPL-glutamateATPMg(2+)L-glutamateYNMNEIKGIERAVQVEVPRPRFLLLAFTDINGSLKGMEIPMERYEEAVEDGVSFDGSSIPGFEGIEDSDLIFKADPSTYAEIPWEGIGRVYGYIYKGDEPYQADPRGILKRVLERLEKEGLKAHIGPEPEFYIFKKNGTWELHIPDSGGYFDLVGLDKAREIRREIALYMPYLGLKPEVLHHEVGKAQHEIDFRYDEALRTADNIVSFKHVVKAVAELHGYYATFMPKPIYGFPGNGMHLHISLWKDGENVFIGEDGLSDTALHFIGGILKHAKALAALTNPTVNSYKRLVPGYEAPVYISWGYRNRSALIRVPAFKGSGARIEYRCPDPSANPYLALAGILMVGLDGIKKKVEPDSYVETNVYEMDDAERERLGIDTLPGSLGEALEELKKDKTVREALGGAYKNFIDYKEREWEEYIEYLSSRDIPIDTKKVTEWELERYFYV
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License