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Protein

Glutamine synthetase

Gene

glnA

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Carries out the ATP-dependent synthesis of glutamine from ammonium nitrogen and glutamate. Exhibits both L-gamma-glutamylhydroxamate synthetase and gamma-glutamyltransferase activities when using hydroxylamine as substrate; in fact, the enzyme possesses low biosynthetic activity, suggesting that the reaction is biased towards the degradation of glutamine under ammonia-rich conditions. Might play some role in ammonia assimilation under ammonia-starvation conditions. Can also use GTP instead of ATP in the synthetase reaction, but not CTP or UTP.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.1 Publication
ATP + L-glutamate + hydroxylamine = ADP + phosphate + L-gamma-glutamylhydroxamate.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Binds 2 Mg2+ or Mn2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme is not controlled by adenylation.1 Publication

Kineticsi

kcat is 2190 min(-1) towards L-glutamate in the synthetase reaction and 3900 min(-1) towards L-glutamine in the transferase reaction.
  1. KM=23.5 mM for L-glutamate (at pH 7.8 and 60 degrees Celsius)1 Publication
  2. KM=15.2 mM for hydroxylamine (when a high concentration is used, at pH 7.8 and 60 degrees Celsius)1 Publication
  3. KM=1.6 mM for hydroxylamine (when a low concentration is used, at pH 7.8 and 60 degrees Celsius)1 Publication
  4. KM=28.0 mM for ATP (at pH 7.8 and 60 degrees Celsius)1 Publication
  5. KM=5.0 mM for L-glutamine (at pH 7.2 and 60 degrees Celsius)1 Publication
  6. KM=6.3 mM for ADP (at pH 7.2 and 60 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.8 for the synthetase reaction and pH 7.2 for the transferase reaction.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius for both transferase and synthetase activities.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi126Magnesium 1By similarity1
    Metal bindingi128Magnesium 2By similarity1
    Binding sitei176ATPBy similarity1
    Metal bindingi181Magnesium 2By similarity1
    Metal bindingi188Magnesium 2By similarity1
    Binding sitei233L-glutamate; via carbonyl oxygenBy similarity1
    Metal bindingi237Magnesium 1; via pros nitrogenBy similarity1
    Binding sitei241ATPBy similarity1
    Binding sitei287L-glutamateBy similarity1
    Binding sitei293L-glutamateBy similarity1
    Binding sitei305ATPBy similarity1
    Binding sitei305L-glutamateBy similarity1
    Binding sitei310ATPBy similarity1
    Metal bindingi322Magnesium 1By similarity1
    Binding sitei324L-glutamateBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi239 – 241ATPBy similarity3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionLigase
    LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTKOD69014:G1G2A-1807-MONOMER
    BRENDAi6.3.1.2 5027

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase1 Publication (EC:6.3.1.21 Publication)
    Short name:
    GS1 Publication
    Alternative name(s):
    Glutamate--ammonia ligaseCurated
    Glutamine synthetase I alphaCurated
    Short name:
    GSI alphaCurated
    Gene namesi
    Name:glnA1 Publication
    Ordered Locus Names:TK1796
    OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
    Taxonomic identifieri69014 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    Proteomesi
    • UP000000536 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001532111 – 443Glutamine synthetaseAdd BLAST443

    Proteomic databases

    PRIDEiO08467

    Interactioni

    Subunit structurei

    Oligomer of 12 subunits arranged in the form of two hexagons.1 Publication

    Protein-protein interaction databases

    IntActiO08467, 1 interactor
    MINTiO08467
    STRINGi69014.TK1796

    Structurei

    3D structure databases

    ProteinModelPortaliO08467
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiarCOG01909 Archaea
    COG0174 LUCA
    HOGENOMiHOG000005156
    InParanoidiO08467
    KOiK01915
    OMAiIEAAWNT
    OrthoDBiPOG093Z00GE

    Family and domain databases

    Gene3Di3.10.20.70, 1 hit
    InterProiView protein in InterPro
    IPR008147 Gln_synt_b-grasp
    IPR036651 Gln_synt_N
    IPR014746 Gln_synth/guanido_kin_cat_dom
    IPR008146 Gln_synth_cat_dom
    IPR027303 Gln_synth_gly_rich_site
    IPR004809 Gln_synth_I
    IPR027302 Gln_synth_N_conserv_site
    PfamiView protein in Pfam
    PF00120 Gln-synt_C, 1 hit
    PF03951 Gln-synt_N, 1 hit
    SMARTiView protein in SMART
    SM01230 Gln-synt_C, 1 hit
    SUPFAMiSSF54368 SSF54368, 1 hit
    SSF55931 SSF55931, 1 hit
    TIGRFAMsiTIGR00653 GlnA, 1 hit
    PROSITEiView protein in PROSITE
    PS00180 GLNA_1, 1 hit
    PS00181 GLNA_ATP, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O08467-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNEIKGIERA VQVEVPRPRF LLLAFTDING SLKGMEIPME RYEEAVEDGV
    60 70 80 90 100
    SFDGSSIPGF EGIEDSDLIF KADPSTYAEI PWEGIGRVYG YIYKGDEPYQ
    110 120 130 140 150
    ADPRGILKRV LERLEKEGLK AHIGPEPEFY IFKKNGTWEL HIPDSGGYFD
    160 170 180 190 200
    LVGLDKAREI RREIALYMPY LGLKPEVLHH EVGKAQHEID FRYDEALRTA
    210 220 230 240 250
    DNIVSFKHVV KAVAELHGYY ATFMPKPIYG FPGNGMHLHI SLWKDGENVF
    260 270 280 290 300
    IGEDGLSDTA LHFIGGILKH AKALAALTNP TVNSYKRLVP GYEAPVYISW
    310 320 330 340 350
    GYRNRSALIR VPAFKGSGAR IEYRCPDPSA NPYLALAGIL MVGLDGIKKK
    360 370 380 390 400
    VEPDSYVETN VYEMDDAERE RLGIDTLPGS LGEALEELKK DKTVREALGG
    410 420 430 440
    AYKNFIDYKE REWEEYIEYL SSRDIPIDTK KVTEWELERY FYV
    Length:443
    Mass (Da):50,308
    Last modified:March 29, 2005 - v2
    Checksum:i94357E45D5F18168
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti402Y → N in BAA20530 (PubMed:9172372).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D86222 Genomic DNA Translation: BAA20530.1
    AP006878 Genomic DNA Translation: BAD85985.1
    RefSeqiWP_011250747.1, NC_006624.1

    Genome annotation databases

    EnsemblBacteriaiBAD85985; BAD85985; TK1796
    GeneIDi3233803
    KEGGitko:TK1796
    PATRICifig|69014.16.peg.1752

    Similar proteinsi

    Entry informationi

    Entry nameiGLN1A_THEKO
    AccessioniPrimary (citable) accession number: O08467
    Secondary accession number(s): Q5JJ67
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: March 29, 2005
    Last modified: May 23, 2018
    This is version 111 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
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    Main funding by: National Institutes of Health