ID   O08452_9EURY            Unreviewed;       460 AA.
AC   O08452;
DT   01-JUL-1997, integrated into UniProtKB/TrEMBL.
DT   01-JUL-1997, sequence version 1.
DT   27-MAR-2024, entry version 122.
DE   SubName: Full=Alpha-amylase {ECO:0000313|EMBL:AAB67705.1, ECO:0000313|EMBL:AAC45663.1};
DE            EC=3.2.1.1 {ECO:0000313|EMBL:AAC45663.1};
GN   Name=amyA {ECO:0000313|EMBL:AAC45663.1};
OS   Pyrococcus furiosus.
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=2261 {ECO:0000313|EMBL:AAC45663.1};
RN   [1] {ECO:0000313|EMBL:AAC45663.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DSM 3638 {ECO:0000313|EMBL:AAC45663.1};
RX   PubMed=9293008;
RA   Dong G., Vieille C., Savchenko A., Zeikus J.G.;
RT   "Cloning, sequencing, and expression of the gene encoding extracellular
RT   alpha-amylase from Pyrococcus furiosus and biochemical characterization of
RT   the recombinant enzyme.";
RL   Appl. Environ. Microbiol. 63:3569-3576(1997).
RN   [2] {ECO:0000313|EMBL:AAB67705.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DSM3638 {ECO:0000313|EMBL:AAB67705.1};
RX   PubMed=9195939; DOI=10.1074/jbc.272.26.16335;
RA   Jorgensen S., Vorgias C.E., Antranikian G.;
RT   "Cloning, sequencing, characterization, and expression of an extracellular
RT   alpha-amylase from the hyperthermophilic archaeon Pyrococcus furiosus in
RT   Escherichia coli and Bacillus subtilis.";
RL   J. Biol. Chem. 272:16335-16342(1997).
RN   [3] {ECO:0000313|EMBL:AAB67705.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DSM3638 {ECO:0000313|EMBL:AAB67705.1};
RA   Joergensen S.T., Vorgias C.E., Antranikian G.;
RL   Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
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DR   EMBL; U96622; AAB67705.1; -; Genomic_DNA.
DR   EMBL; AF001268; AAC45663.1; -; Genomic_DNA.
DR   RefSeq; WP_014835153.1; NC_018092.1.
DR   AlphaFoldDB; O08452; -.
DR   SMR; O08452; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   GeneID; 41712278; -.
DR   BRENDA; 3.2.1.1; 5243.
DR   EvolutionaryTrace; O08452; -.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR015237; Alpha-amylase_C_pro.
DR   InterPro; IPR006046; Alpha_amylase.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF09154; Alpha-amy_C_pro; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR   PRINTS; PR00110; ALPHAAMYLASE.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000313|EMBL:AAC45663.1};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:AAC45663.1}.
FT   DOMAIN          35..380
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   460 AA;  52910 MW;  0E0A2AEB5FCAF541 CRC64;
     MNIKKLTPLL TLLLFFIVLA SPVSAAKYLE LEEGGVIMQA FYWDVPGGGI WWDHIRSKIP
     EWYEAGISAI WLPPPSKGMS GGYSMGYDPY DYFDLGEYYQ KGTVETRFGS KEELVRLIQT
     AHAYGIKVIA DVVINHRAGG DLEWNPFVGD YTWTDFSKVA SGKYTANYLD FHPNELHCCD
     EGTFGGFPDI CHHKEWDQYW LWKSNESYAA YLRSIGFDGW RFDYVKGYGA WVVRDWLNWW
     GGWAVGEYWD TNVDALLSWA YESGAKVFDF PLYYKMDEAF DNNNIPALVY ALQNGQTVVS
     RDPFKAVTFV ANHDTDIIWN KYPAYAFILT YEGQPVIFYR DFEEWLNKDK LINLIWIHDH
     LAGGSTTIVY YDNDELIFVR NGDSRRPGLI TYINLSPNWV GRWVYVPKFA GACIHEYTGN
     LGGWVDKRVD SSGWVYLEAP PHDPANGYYG YSVWSYCGVG
//