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O08452

- O08452_9EURY

UniProt

O08452 - O08452_9EURY

Protein
Submitted name:

Alpha amylase

Gene

amyA

Organism
Pyrococcus furiosus
Status
Unreviewed - Annotation score: 1 out of 5- Protein predictedi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi49 – 491Magnesium 1; via carbonyl oxygen
    Metal bindingi105 – 1051Magnesium 1
    Metal bindingi113 – 1131Magnesium 1
    Metal bindingi277 – 2771Magnesium 2
    Metal bindingi281 – 2811Magnesium 2
    Metal bindingi317 – 3171Magnesium 2; via carbonyl oxygen
    Metal bindingi372 – 3721Magnesium 3
    Metal bindingi374 – 3741Magnesium 3
    Metal bindingi375 – 3751Magnesium 3

    GO - Molecular functioni

    1. alpha-amylase activity Source: UniProtKB-EC
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseImported, Hydrolase

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Alpha amylaseImported (EC:3.2.1.1Imported)
    Submitted name:
    Alpha-amylaseImported
    Gene namesi
    Name:amyAImported
    OrganismiPyrococcus furiosusImported
    Taxonomic identifieri2261 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

    PTM / Processingi

    Proteomic databases

    PRIDEiO08452.

    Structurei

    3D structure databases

    ProteinModelPortaliO08452.
    SMRiO08452. Positions 26-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO08452.

    Family & Domainsi

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013776. A-amylase_thermo.
    IPR015237. Alpha-amylase_C_pro.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF09154. DUF1939. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
    PRINTSiPR00110. ALPHAAMYLASE.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O08452-1 [UniParc]FASTAAdd to Basket

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    MNIKKLTPLL TLLLFFIVLA SPVSAAKYLE LEEGGVIMQA FYWDVPGGGI    50
    WWDHIRSKIP EWYEAGISAI WLPPPSKGMS GGYSMGYDPY DYFDLGEYYQ 100
    KGTVETRFGS KEELVRLIQT AHAYGIKVIA DVVINHRAGG DLEWNPFVGD 150
    YTWTDFSKVA SGKYTANYLD FHPNELHCCD EGTFGGFPDI CHHKEWDQYW 200
    LWKSNESYAA YLRSIGFDGW RFDYVKGYGA WVVRDWLNWW GGWAVGEYWD 250
    TNVDALLSWA YESGAKVFDF PLYYKMDEAF DNNNIPALVY ALQNGQTVVS 300
    RDPFKAVTFV ANHDTDIIWN KYPAYAFILT YEGQPVIFYR DFEEWLNKDK 350
    LINLIWIHDH LAGGSTTIVY YDNDELIFVR NGDSRRPGLI TYINLSPNWV 400
    GRWVYVPKFA GACIHEYTGN LGGWVDKRVD SSGWVYLEAP PHDPANGYYG 450
    YSVWSYCGVG 460
    Length:460
    Mass (Da):52,910
    Last modified:July 1, 1997 - v1
    Checksum:i0E0A2AEB5FCAF541
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U96622 Genomic DNA. Translation: AAB67705.1.
    AF001268 Genomic DNA. Translation: AAC45663.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U96622 Genomic DNA. Translation: AAB67705.1 .
    AF001268 Genomic DNA. Translation: AAC45663.1 .

    3D structure databases

    ProteinModelPortali O08452.
    SMRi O08452. Positions 26-460.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PRIDEi O08452.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei O08452.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR013776. A-amylase_thermo.
    IPR015237. Alpha-amylase_C_pro.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF09154. DUF1939. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001021. Alph-amls_thrmst. 1 hit.
    PRINTSi PR00110. ALPHAAMYLASE.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing, and expression of the gene encoding extracellular alpha-amylase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme."
      Dong G., Vieille C., Savchenko A., Zeikus J.G.
      Appl. Environ. Microbiol. 63:3569-3576(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: DSM 3638Imported.
    2. "Cloning, sequencing, characterization, and expression of an extracellular alpha-amylase from the hyperthermophilic archaeon Pyrococcus furiosus in Escherichia coli and Bacillus subtilis."
      Jorgensen S., Vorgias C.E., Antranikian G.
      J. Biol. Chem. 272:16335-16342(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: DSM3638Imported.
    3. Joergensen S.T., Vorgias C.E., Antranikian G.
      Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: DSM3638Imported.

    Entry informationi

    Entry nameiO08452_9EURY
    AccessioniPrimary (citable) accession number: O08452
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 1, 1997
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)