O08451 (ACON_MYCAV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aconitate hydratase
Short name=Aconitase
EC=4.2.1.3

Alternative name(s):
Citrate hydro-lyase

Gene names

Name:

acn

Organism

Mycobacterium avium

Taxonomic identifier

1764 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	961 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization of citrate to isocitrate via cis-aconitate By similarity.
Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC citrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC isocitrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 961	961	Aconitate hydratase		PRO_0000076663
Sites
	Metal binding	499	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	565	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	568	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08451 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 689E66D85E31F596	FASTA	961	104,025
	10 20 30 40 50 60 MQDKRTVKVG TVADTAVGGR TKSLGVEVTD SVNSFGARNR VKVGDKSYQI YRLDAVPNTE 70 80 90 100 110 120 KLPYSLKVLA ENLLRNEDGS NITKDHIEAI ANWDPKAGAS IEIQYTPARV VMQDFTGVPC 130 140 150 160 170 180 IVDLATMREA IADLGGNPEK VNPLAPADLV IDHSVIADLF GTADAFERNV EIEYQRNGER 190 200 210 220 230 240 YQFLRWLQGA FSDFKVVPPR RIVHQVNIEY LARVVMERVG VAYPDTCVGT DSHTTMVNGL 250 260 270 280 290 300 GVLGWGVGGI EAEAAMLGQP VSMLIPRVVG FKLTGEDPAP GAATDVVLTV TEICAKHGVV 310 320 330 340 350 360 GKFVEFYGEG VAEVPLANRA TLGNMSPEFG STAAIFPIDQ ETIDYLKFTG RNAEQVALVE 370 380 390 400 410 420 TYAKEQGLWH DPAHEPAFSE YLELDLSQVV PSIAGPKRPQ DRIALSQAKS VFREQIPSYV 430 440 450 460 470 480 GDGDGQQGYS KLDEVVDETF PASDPGAPSN GHADDLPAVQ SAAAHANGRP SNPVTVRSDE 490 500 510 520 530 540 LGEFVLDHGA VVIAAVTSCT NTSNPEVMLG AALLARQRVE KGLASKPWVK TTMAPGSQVV 550 560 570 580 590 600 HDYYDKAGLW PYLEKLGFYL VGYGCTTCIG NSGPLPEEIS KAINDNDLSV TAVLSGNRNF 610 620 630 640 650 660 EGRINPDVKM NYPASPPLVV AYALAGTMTR LEKQPLGKDK DGNDVYLKDI CRSQKTLGHH 670 680 690 700 710 720 PIGDNSEWFT KNYADVFKGE QAWRNLPTPT RNTFEWSPDS TYVRKPPYFE GMPAEPEPVA 730 740 750 760 770 780 DISSARVVAL LGDSVTTDHI SPAGSIKPGT PAAQYLDDAR RGPQGLQLFG CRRGNHEVMI 790 800 810 820 830 840 RGTFANIRLR NLLHDDVAGG YTRDFTQDGG PQAFIYDAAQ NYAAQNIPLV VLGGKEYGSG 850 860 870 880 890 900 SSRDWAAKGT RLLGVRAVIA ESFERIHRSN LIGMGVIPLQ FPDGKSAKDL GLDGTEVFDI 910 920 930 940 950 960 TGIEELNKGK TPKTVHVKAS KNGSDAAEFD AVVRIDTPGE ADYYRNGGIL QYVLRNMLKS G

References

[1]

"Determination of a 15437 bp nucleotide sequence around the inhA gene of Mycobacterium avium and similarity analysis of the products of putative ORFs." Labo M., Gusberti L., de Rossi E., Speziale P., Riccardi G. Microbiology 144:807-814(1998) [PubMed: 9534249] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GIR10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF002133 Genomic DNA. Translation: AAC46192.1.
3D structure databases
ProteinModelPortal	O08451.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR015937. Acoase/IPM_deHydtase. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 3 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 2 hits. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit. SSF53732. Aconitase_N. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_MYCAV

Accession

Primary (citable) accession number: O08451

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	September 21, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program