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O08450

- DEF_CLOB8

UniProt

O08450 - DEF_CLOB8

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Protein

Peptide deformylase

Gene

def

Organism
Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity).By similarity

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.

Cofactori

Binds 1 Fe2+ ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851IronBy similarity
Metal bindingi126 – 1261IronBy similarity
Active sitei127 – 1271By similarity
Metal bindingi130 – 1301IronBy similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciCBEI290402:GHL5-3610-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylase (EC:3.5.1.88)
Short name:
PDF
Alternative name(s):
Polypeptide deformylase
Gene namesi
Name:def
Synonyms:fms
Ordered Locus Names:Cbei_3511
OrganismiClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
Taxonomic identifieri290402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000565: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 136136Peptide deformylasePRO_0000082768Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290402.Cbei_3511.

Structurei

3D structure databases

ProteinModelPortaliO08450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.Curated

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243509.
KOiK01462.
OMAiHEMDHFE.
OrthoDBiEOG6FNHTX.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.

Sequencei

Sequence statusi: Complete.

O08450-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKPIVKDIL FLGQKSEEAT KNDMVVIDDL IDTLRANLEH CVGLAANMIG
60 70 80 90 100
VKKRILVFTV GNLIVPMINP VILKKEKPYE TEESCLSLIG FRKTKRYETI
110 120 130
EVTYLDRNFN KKKQVFNGFT AQIIQHEMDH FEGIII
Length:136
Mass (Da):15,623
Last modified:July 1, 1997 - v1
Checksum:i7E9CE063FFDE9BE6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z96934 Genomic DNA. Translation: CAB09662.1.
CP000721 Genomic DNA. Translation: ABR35634.1.
RefSeqiWP_012059684.1. NC_009617.1.
YP_001310590.1. NC_009617.1.

Genome annotation databases

EnsemblBacteriaiABR35634; ABR35634; Cbei_3511.
GeneIDi5294680.
KEGGicbe:Cbei_3511.
PATRICi19351248. VBICloBei69853_3642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z96934 Genomic DNA. Translation: CAB09662.1 .
CP000721 Genomic DNA. Translation: ABR35634.1 .
RefSeqi WP_012059684.1. NC_009617.1.
YP_001310590.1. NC_009617.1.

3D structure databases

ProteinModelPortali O08450.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290402.Cbei_3511.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR35634 ; ABR35634 ; Cbei_3511 .
GeneIDi 5294680.
KEGGi cbe:Cbei_3511.
PATRICi 19351248. VBICloBei69853_3642.

Phylogenomic databases

eggNOGi COG0242.
HOGENOMi HOG000243509.
KOi K01462.
OMAi HEMDHFE.
OrthoDBi EOG6FNHTX.

Enzyme and pathway databases

BioCyci CBEI290402:GHL5-3610-MONOMER.

Family and domain databases

Gene3Di 3.90.45.10. 1 hit.
HAMAPi MF_00163. Pep_deformylase.
InterProi IPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view ]
PANTHERi PTHR10458. PTHR10458. 1 hit.
Pfami PF01327. Pep_deformylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF004749. Pep_def. 1 hit.
PRINTSi PR01576. PDEFORMYLASE.
SUPFAMi SSF56420. SSF56420. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Truncation of peptide deformylase reduces the growth rate and stabilizes solvent production in Clostridium beijerinckii NCIMB 8052."
    Evans V.J., Liyanage H., Ravagnani A., Young M., Kashket E.R.
    Appl. Environ. Microbiol. 64:1780-1785(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51743 / NCIMB 8052.

Entry informationi

Entry nameiDEF_CLOB8
AccessioniPrimary (citable) accession number: O08450
Secondary accession number(s): A6LZ54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 1, 1997
Last modified: October 1, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3