O08445 (ALR_LACPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	lp_0523

Organism

Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) [Reference proteome] [HAMAP]

Taxonomic identifier

220668 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus plantarum

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Alanine racemase HAMAP-Rule MF_01201		PRO_0000114528
Sites
	Active site	41	1	Proton acceptor; specific for D-alanine By similarity
	Active site	270	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	317	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	41	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08445 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 844E9BAC5FF3463B	FASTA	375	40,710
	10 20 30 40 50 60 MVVIGEHRHT QVTVDLQAIK TNISNEMAQK DELTELWAVV KANGYGHGII QVAQAAKEAG 70 80 90 100 110 120 ATGFCVAILD EALALRAAGF AEPILVLGIT EPEYAPLVAE KDISLAVGTQ DWLTTAAAIL 130 140 150 160 170 180 AANQVTTPLH VHLALDTGMG RIGFQTPEEL ATAVTTLRQP QSPFDFEGIF THFATADQAD 190 200 210 220 230 240 DTYFTHQLNN WKHLIAVVDE LPRYVHVSNS ATSLWHQACN GNMVRFGVAL YGLNPSGREL 250 260 270 280 290 300 SAPYPLQPAL SLTARLTFVK RLARGKSVSY GATYTAAQDE WIGTVPIGYA DGYERRLQGF 310 320 330 340 350 360 HVLVDGEFCE IVGRVCMDQL MVRLPHEVPV GAKVTLVGTD GARTISLQDI ADYCGTIHYE 370 IACGLAPRVP RVYID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The alanine racemase gene is essential for growth of Lactobacillus plantarum." Hols P., Defrenne C., Ferain T., Derzelle S., Delplace B., Delcour J. J. Bacteriol. 179:3804-3807(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.
[2]	"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08941 Genomic DNA. Translation: CAA70140.1. AL935263 Genomic DNA. Translation: CCC78013.1.
RefSeq	YP_004888527.1. NC_004567.2.
3D structure databases
ProteinModelPortal	O08445.
ModBase	Search...
Protein-protein interaction databases
STRING	220668.lp_0523.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CCC78013; CCC78013; lp_0523.
GeneID	1061815.
KEGG	lpl:lp_0523.
PATRIC	22247489. VBILacPla27411_0431.
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000031444.
KO	K01775.
OMA	SVELWGD.
ProtClustDB	CLSK821195.
Enzyme and pathway databases
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_LACPL

Accession

Primary (citable) accession number: O08445
Secondary accession number(s): F9UKZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program