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O08430

- APHA_SALTI

UniProt

O08430 - APHA_SALTI

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Protein

Class B acid phosphatase

Gene

aphA

Organism
Salmonella typhi
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei69 – 691NucleophileBy similarity
Metal bindingi69 – 691MagnesiumBy similarity
Active sitei71 – 711Proton donorBy similarity
Metal bindingi71 – 711Magnesium; via carbonyl oxygenBy similarity
Binding sitei177 – 1771SubstrateBy similarity
Metal bindingi192 – 1921MagnesiumBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphatase (EC:3.1.3.2)
Short name:
CBAP
Gene namesi
Name:aphA
Synonyms:napA
Ordered Locus Names:STY4445, t4155
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000000541: Chromosome, UP000002670: Chromosome

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 237214Class B acid phosphatasePRO_0000024008Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi220341.STY4445.

Structurei

3D structure databases

ProteinModelPortaliO08430.
SMRiO08430. Positions 27-237.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1382Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3700.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.
OrthoDBiEOG6HTNVZ.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08430-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKITLALSA VCLLFTLNHS ANALVSSPST LNPGTNVAKL AEQAPVHWVS
60 70 80 90 100
VAQIENSLTG RPPMAVGFDI DDTVLFSSPG FWRGKKTYSP DSDDYLKNPA
110 120 130 140 150
FWEKMNNGWD EFSIPKEVAR QLIDMHVRRG DSIYFVTGRS QTKTETVSKT
160 170 180 190 200
LADNFHIPAA NMNPVIFAGD KPGQNTKVQW LQEKNMRIFY GDSDNDITAA
210 220 230
RDCGIRGIRI LRAANSTYKP LPQAGAFGEE VIVNSEY
Length:237
Mass (Da):26,243
Last modified:December 5, 2001 - v2
Checksum:i3B0D71DFE7A16269
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71Missing in CAB40974. (PubMed:10564784)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237788 Genomic DNA. Translation: CAB40974.1.
AL513382 Genomic DNA. Translation: CAD09233.1.
AE014613 Genomic DNA. Translation: AAO71619.1.
RefSeqiNP_458547.1. NC_003198.1.
NP_807759.1. NC_004631.1.

Genome annotation databases

EnsemblBacteriaiAAO71619; AAO71619; t4155.
CAD09233; CAD09233; CAD09233.
GeneIDi1250662.
KEGGisty:STY4445.
PATRICi18547134. VBISalEnt120419_4546.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237788 Genomic DNA. Translation: CAB40974.1 .
AL513382 Genomic DNA. Translation: CAD09233.1 .
AE014613 Genomic DNA. Translation: AAO71619.1 .
RefSeqi NP_458547.1. NC_003198.1.
NP_807759.1. NC_004631.1.

3D structure databases

ProteinModelPortali O08430.
SMRi O08430. Positions 27-237.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 220341.STY4445.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO71619 ; AAO71619 ; t4155 .
CAD09233 ; CAD09233 ; CAD09233 .
GeneIDi 1250662.
KEGGi sty:STY4445.
PATRICi 18547134. VBISalEnt120419_4546.

Phylogenomic databases

eggNOGi COG3700.
HOGENOMi HOG000270623.
KOi K03788.
OMAi PEFWEKM.
OrthoDBi EOG6HTNVZ.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view ]
Pfami PF03767. Acid_phosphat_B. 1 hit.
[Graphical view ]
PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01672. AphA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic rearrangements in the tyrB-uvrA region of the enterobacterial chromosome: a potential cause for different class B acid phosphatase regulation in Salmonella enterica and Escherichia coli."
    Rossolini G.M., Bonci A., Schippa S., Berlutti F., Thaller M.C.
    FEMS Microbiol. Lett. 181:17-23(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: STY4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CT18.
  3. "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18."
    Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.
    J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700931 / Ty2.

Entry informationi

Entry nameiAPHA_SALTI
AccessioniPrimary (citable) accession number: O08430
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 5, 2001
Last modified: November 26, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3