Reviewed, UniProtKB/Swiss-Prot O08430 (APHA_SALTI)

Names and origin

Protein names

Recommended name:
    Class B acid phosphatase
    EC=3.1.3.2

Gene names

Name:	aphA
Synonyms:	napA
Ordered Locus Names:	STY4445, t4155

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Dephosphorylates several organic phosphomonoesters and catalyzes the transfer of low-energy phosphate groups from phosphomonoesters to hydroxyl groups of various organic compounds. Might function as a broad spectrum dephosphorylating enzyme able to scavenge both 3'- and 5'-nucleotides and also additional organic phosphomonoesters By similarity.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the class B bacterial acid phosphatase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 237	214	Class B acid phosphatase		PRO_0000024008
Sites
	Metal binding	69	1	Magnesium By similarity
	Metal binding	71	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	192	1	Magnesium By similarity
Experimental info
	Sequence conflict	7	1	Missing in CAB40974. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O08430-1 [UniParc]. Last modified December 5, 2001. Version 2. Checksum: 3B0D71DFE7A16269	FASTA	237	26,243
	10 20 30 40 50 60 MKKITLALSA VCLLFTLNHS ANALVSSPST LNPGTNVAKL AEQAPVHWVS VAQIENSLTG 70 80 90 100 110 120 RPPMAVGFDI DDTVLFSSPG FWRGKKTYSP DSDDYLKNPA FWEKMNNGWD EFSIPKEVAR 130 140 150 160 170 180 QLIDMHVRRG DSIYFVTGRS QTKTETVSKT LADNFHIPAA NMNPVIFAGD KPGQNTKVQW 190 200 210 220 230 LQEKNMRIFY GDSDNDITAA RDCGIRGIRI LRAANSTYKP LPQAGAFGEE VIVNSEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic rearrangements in the tyrB-uvrA region of the enterobacterial chromosome: a potential cause for different class B acid phosphatase regulation in Salmonella enterica and Escherichia coli." Rossolini G.M., Bonci A., Schippa S., Berlutti F., Thaller M.C. FEMS Microbiol. Lett. 181:17-23(1999) [PubMed: 10564784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: STY4.
[2]	"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.
[3]	"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ237788 Genomic DNA. Translation: CAB40974.1. AL627282 Genomic DNA. Translation: CAD09233.1. AE014613 Genomic DNA. Translation: AAO71619.1.
RefSeq	NP_458547.1. NP_807759.1.
3D structure databases
SMR	O08430. Positions 27-237.
ModBase	Search...
Genome annotation databases
GeneID	1069720. 1250662.
GenomeReviews	Gene locus t4155 in contig AE014613_GR.
KEGG	stt:t4155. sty:STY4445.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG698065.
OMA	GQNTKTQ.
Enzyme and pathway databases
BioCyc	SENT209261:T4155-MONOMER. SENT220341:STY4445-MONOMER.
BRENDA	3.1.3.2. 3716.
Family and domain databases
InterPro	IPR005519. Acid_phosphat_B. IPR010025. HAD-SF_ppase_IIIB_AphA. [Graphical view]
Pfam	PF03767. Acid_phosphat_B. 1 hit. [Graphical view]
PIRSF	PIRSF017818. Acid_Ptase_B. 1 hit.
TIGRFAMs	TIGR01672. AphA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APHA_SALTI

Accession

Primary (citable) accession number: O08430

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 5, 2001
Last modified:	February 9, 2010
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)