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Protein

Class B acid phosphatase

Gene

aphA

Organism
Salmonella typhi
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates several organic phosphate monoesters including monophosphate nucleotides (NMPs), coenzyme A (CoA), nicotinamide adenine dinucleotide phosphate (NADP), flavin mononucleotide (FMN) and phosphorylated 5-6 carbon sugars in vitro (PubMed:25848029). Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69NucleophileBy similarity1
Metal bindingi69MagnesiumBy similarity1
Active sitei71Proton donorBy similarity1
Metal bindingi71Magnesium; via carbonyl oxygenBy similarity1
Binding sitei177SubstrateBy similarity1
Metal bindingi192MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphatase (EC:3.1.3.21 Publication)
Short name:
CBAP
Gene namesi
Name:aphA
Synonyms:napA
Ordered Locus Names:STY4445, t4155
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000002400824 – 237Class B acid phosphataseAdd BLAST214

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi220341.STY4445.

Structurei

3D structure databases

ProteinModelPortaliO08430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 138Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKITLALSA VCLLFTLNHS ANALVSSPST LNPGTNVAKL AEQAPVHWVS
60 70 80 90 100
VAQIENSLTG RPPMAVGFDI DDTVLFSSPG FWRGKKTYSP DSDDYLKNPA
110 120 130 140 150
FWEKMNNGWD EFSIPKEVAR QLIDMHVRRG DSIYFVTGRS QTKTETVSKT
160 170 180 190 200
LADNFHIPAA NMNPVIFAGD KPGQNTKVQW LQEKNMRIFY GDSDNDITAA
210 220 230
RDCGIRGIRI LRAANSTYKP LPQAGAFGEE VIVNSEY
Length:237
Mass (Da):26,243
Last modified:December 5, 2001 - v2
Checksum:i3B0D71DFE7A16269
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7Missing in CAB40974 (PubMed:10564784).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237788 Genomic DNA. Translation: CAB40974.1.
AL513382 Genomic DNA. Translation: CAD09233.1.
AE014613 Genomic DNA. Translation: AAO71619.1.
RefSeqiNP_458547.1. NC_003198.1.
WP_000724436.1. NZ_LUHL01000018.1.

Genome annotation databases

EnsemblBacteriaiAAO71619; AAO71619; t4155.
CAD09233; CAD09233; CAD09233.
GeneIDi1250662.
KEGGistt:t4155.
sty:STY4445.
PATRICi18547134. VBISalEnt120419_4546.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237788 Genomic DNA. Translation: CAB40974.1.
AL513382 Genomic DNA. Translation: CAD09233.1.
AE014613 Genomic DNA. Translation: AAO71619.1.
RefSeqiNP_458547.1. NC_003198.1.
WP_000724436.1. NZ_LUHL01000018.1.

3D structure databases

ProteinModelPortaliO08430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220341.STY4445.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO71619; AAO71619; t4155.
CAD09233; CAD09233; CAD09233.
GeneIDi1250662.
KEGGistt:t4155.
sty:STY4445.
PATRICi18547134. VBISalEnt120419_4546.

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPHA_SALTI
AccessioniPrimary (citable) accession number: O08430
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 5, 2001
Last modified: November 2, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.