Reviewed, UniProtKB/Swiss-Prot O08424 (HMDH_SULSO)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:	hmgA
Ordered Locus Names:	SSO0531
ORF Names:	C22_020

Organism

Sulfolobus solfataricus [Complete proteome] [HAMAP]

Taxonomic identifier

2287 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts HMG-CoA to mevalonate.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Sequence similarities	Belongs to the HMG-CoA reductase family.
Biophysicochemical properties	pH dependence: Optimum pH is 5.5. Temperature dependence: Optimum temperature is 85 degrees Celsius. Stable at 90 degrees Celsius.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114465
Sites
	Active site	99	1	Charge relay system By similarity
	Active site	305	1	Charge relay system By similarity
	Active site	400	1	Proton donor By similarity
Experimental info
	Sequence conflict	245	1	V → L in AAC45370. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O08424-1 [UniParc]. Last modified June 20, 2001. Version 2. Checksum: 8D1FA64E117CFF46	FASTA	409	44,009
	10 20 30 40 50 60 MKIDEVVEKL VKGEISFHEV DNLLEANAAM VARRLALEKI VGVGLPSIGS TVIDYSEIKN 70 80 90 100 110 120 KNAENVIGAI QIPLGIVGPI RVNGDYAKGD FYVPMATTEG ALIASVNRGI KAVTLSGGVR 130 140 150 160 170 180 AKVLKDEMTR APVFKFDSIE QIPNFLKFIE ENLEKIRNIA NSTSHHGKLK SITPFVLGNN 190 200 210 220 230 240 VWLRFSFETG DAMGMNMVTI AVEKVCEFIE ENFPSADCLA VSGNMCSDKK QTNVNSLFGR 250 260 270 280 290 300 GKTVVAEALI KKDVIRNILH SNAQLIHDIN LRKNWLGTAR AGSLSQFNAH FANIVTAIFI 310 320 330 340 350 360 ATGQDVAQIV ESSSGYTWTE VRGEDLYISV TLPSLEVGTV GGGTRLPTQK EALSIMGVYG 370 380 390 400 SGNPPGSNAK KLAEIIASTV LSGELNLLAA LSNKELGKAH AKLGRAMKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"3-hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic characterization of the gene product." Bochar D.A., Brown J.R., Doolittle W.F., Klenk H.-P., Lam W., Schenk M.E., Stauffacher C.V., Rodwell V.W. J. Bacteriol. 179:3632-3638(1997) [PubMed: 9171410] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2." Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W. Genome 43:116-136(2000) [PubMed: 10701121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U95360 Genomic DNA. Translation: AAC45370.1. Y18930 Genomic DNA. Translation: CAB57768.1. AE006641 Genomic DNA. Translation: AAK40851.1.
PIR	D90199. T51306.
RefSeq	NP_342061.1.
3D structure databases
HSSP	HSSP built from PDB template 1HWI based on UniProtKB P04035.
ModBase	Search...
Genome annotation databases
GeneID	1454818.
GenomeReviews	Gene locus SSO0531 in contig AE006641_GR.
KEGG	sso:SSO0531.
NMPDR	fig|273057.1.peg.484.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O08424.
OMA	AHFANII.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0531-MON.
BRENDA	1.1.1.34. 2070.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. False negative. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_SULSO

Accession

Primary (citable) accession number: O08424
Secondary accession number(s): Q9UWT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 20, 2001
Last modified:	November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)