Reviewed, UniProtKB/Swiss-Prot O08405 (KATG2_MYCFO)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Synonyms:	katGII

Organism

Mycobacterium fortuitum

Taxonomic identifier

1766 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 733	733	Catalase-peroxidase 2 HAMAP MF_01961		PRO_0000345093
Sites
	Active site	107	1	Proton acceptor By similarity
	Metal binding	275	1	Iron (heme axial ligand) By similarity
	Site	103	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	106 ↔ 234		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-260) By similarity
	Cross-link	234 ↔ 260		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-106) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08405-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 20C8BCB076F9E58F	FASTA	733	80,596
	10 20 30 40 50 60 MAEAETHPPI GESQTEPAES GCPMRIKPPV EGGSNRDWWP NAVNLKILQK NPPAIDPSDE 70 80 90 100 110 120 GYDSEAVKSL DVEAFQRDFD ELLTNSQDWW PADFGHYGPL FVRMSWHAAG TYRVEDGRGG 130 140 150 160 170 180 GGRGMQPFAP LNSWPDNVSL DKARRLLWPL KKKYGKQISW SDLIVYSGNR AMEHMGFKTA 190 200 210 220 230 240 GFAFGRPDYW EPEEDIYWGA EAEWLGSQDR YAGANGDRTK LENPPXXPHM GLIYVNPEGP 250 260 270 280 290 300 EGNPDYLAAA IDIRETFGRM AMNDIETAAL IVGGHTFGKT HGATDIENGV EPEXXPLEQM 310 320 330 340 350 360 GLGWANPGLG NDTVSSGLEV TWTQHPTKWD NSFLEILYSN EWELTKSPAG ANQWKPKDNG 370 380 390 400 410 420 WANSWPMAQG TGKTHPSMLT TDLSMRFDPI YGEITRRWLD HPEELAEEYA KAWFKLIHRD 430 440 450 460 470 480 MGPVTRYLGP LVPKQTWLWQ DIIPAGKQLS DADVATLKAA IADSGLSIQQ LVNTAWKAAA 490 500 510 520 530 540 SYRSSDMRGG NGGRIRLQPQ LGWEVNEPEE LAPVIAKLEE IQAASDSGVS FADLVVLGGV 550 560 570 580 590 600 VGLEKAIKAA GFDVAVPFTS GPRDALQEQT DVDSFAYLEP KGDGFRNFVA KGDSVPAEYR 610 620 630 640 650 660 LIDRANLLGL SAPQMTVLIG GLRVLGANHG GSELGVLTDK VGQLTNDYFV NLTDMGTKWA 670 680 690 700 710 720 PAPADDGTYV GTDRATGSPK WTASRVDLLF GSNSQLRALA EVYAEDDSKE KFVKDFVAAW 730 TKVMNADRFD LEA

References

[1]

"katGI and katGII encode two different catalases-peroxidases in Mycobacterium fortuitum." Menendez M.C., Ainsa J.A., Martin C., Garcia M.J. J. Bacteriol. 179:6880-6886(1997) [PubMed: 9371430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6841 / DSM 46621 / IFO 13159 / NBRC 13159 / NCTC 10394.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y07866 Genomic DNA. Translation: CAA69193.1.
3D structure databases
HSSP	HSSP built from PDB template 1MWV based on UniProtKB Q939D2.
ModBase	Search...
Protein family/group databases
PeroxiBase	3128. MfoCP02.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_MYCFO

Accession

Primary (citable) accession number: O08405

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 1, 1997
Last modified:	May 5, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)