Reviewed, UniProtKB/Swiss-Prot O08404 (KATG1_MYCFO)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Synonyms:	katGI

Organism

Mycobacterium fortuitum

Taxonomic identifier

1766 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	752 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer. HAMAP MF_01961
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 752	752	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000055572
Sites
	Active site	117	1	Proton acceptor By similarity
	Metal binding	285	1	Iron (heme axial ligand) By similarity
	Site	113	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	116 ↔ 244		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-270) By similarity
	Cross-link	244 ↔ 270		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-116) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08404-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: B66BAD53DD26E70D	FASTA	752	83,101
	10 20 30 40 50 60 MPPNTPDASD ARPPQADTET HSHSESENPV IESPKPKAHA PLTNQDWWPD QVDVSRLHKQ 70 80 90 100 110 120 PIEGNPLGAG FNYAEEFQKL DVEALRADML ELMTSSQDWW PRDYGTYAGL FIRMSWHAAG 130 140 150 160 170 180 TYRIFDGRGG AGQGAQRFAP INSWPDNVSL DKARRLLWPI KQKYGNKISW ADLIIFAGNV 190 200 210 220 230 240 ALESAGFKTF GFAFGRQDIW EPEEILWGQE DTWLGTDKRY GGTNDSTNRE LANPYGATTM 250 260 270 280 290 300 GLIYVNPEGP EGKPDPLAAA HDIRETFGRM AMNDEETAAL IVGGHTLGKT HGPGPGDLVG 310 320 330 340 350 360 PEPEAAPIEQ QGLGWKCAFG SGKGSDTITS GLEVVWTTTP TKWSNSYLEI LYGYEWELTK 370 380 390 400 410 420 SPGDAWQFEA KDAEAIIPDP FGGPPRKPTM LVTDISMRVD PIYGPITRRW LEHPEELNEA 430 440 450 460 470 480 FAKAWYKLLH RDMGPISRYL GPWIPEPQLW QDPVPDVDHP LVDEQDIAAL KEKLLDSGLS 490 500 510 520 530 540 VQQLVKTAWS AAASFRGTDK RGGANGGRLR LQPQRNWEVN EPSELDKALP VLERIAQDFN 550 560 570 580 590 600 ASASDGKKIS LADLIVLGGS AAIEKAARDG GYEVKVHFVA GRTDASQENT DVDSFAVLEP 610 620 630 640 650 660 RADGFRNFVR PGDKAPLEQL LVDKAYFLNL TAPEMTVLVG GLRALNTNHG GSKHGVFTAN 670 680 690 700 710 720 PGALSNDFFV NLLDMSTEWK PSETAENVYE GRDRRTGQTR WTATANDLVF GSNSVLRAVA 730 740 750 EVYAQEDNKA KMIEDFVAAW VKVMNNDRFD LD

References

[1]

"katGI and katGII encode two different catalases-peroxidases in Mycobacterium fortuitum." Menendez M.C., Ainsa J.A., Martin C., Garcia M.J. J. Bacteriol. 179:6880-6886(1997) [PubMed: 9371430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6841 / DSM 46621 / IFO 13159 / NBRC 13159 / NCTC 10394.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y07865 Genomic DNA. Translation: CAA69192.1.
3D structure databases
HSSP	HSSP built from PDB template 1MWV based on UniProtKB Q939D2.
ModBase	Search...
Protein family/group databases
PeroxiBase	2396. MfoCP01.
Enzyme and pathway databases
BRENDA	1.11.1.6. 141585.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_MYCFO

Accession

Primary (citable) accession number: O08404

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 1, 1997
Last modified:	May 5, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)