Catalase-peroxidase 1 - Mycobacterium fortuitum

Preferred order of sections

Names and origin

Protein names

Recommended name:
Catalase-peroxidase 1
Short name=CP 1
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase 1

Gene names

Name:	katG1
Synonyms:	katGI

Organism

Mycobacterium fortuitum

Taxonomic identifier

1766 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	752 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria By similarity. HAMAP MF_01961
Catalytic activity	Donor + H₂O₂ = oxidized donor + 2 H₂O. HAMAP MF_01961 2 H₂O₂ = O₂ + 2 H₂O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 752

752

Catalase-peroxidase 1 HAMAP MF_01961

PRO_0000055572

Sites

Active site

117

1

Proton acceptor By similarity

Metal binding

285

1

Iron (heme axial ligand) By similarity

Site

113

1

Transition state stabilizer By similarity

Amino acid modifications

Cross-link

116 ↔ 244

Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-270) By similarity

Cross-link

244 ↔ 270

Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-116) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O08404 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: B66BAD53DD26E70D
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FASTA

752

83,101

        10         20         30         40         50         60 
MPPNTPDASD ARPPQADTET HSHSESENPV IESPKPKAHA PLTNQDWWPD QVDVSRLHKQ 

        70         80         90        100        110        120 
PIEGNPLGAG FNYAEEFQKL DVEALRADML ELMTSSQDWW PRDYGTYAGL FIRMSWHAAG 

       130        140        150        160        170        180 
TYRIFDGRGG AGQGAQRFAP INSWPDNVSL DKARRLLWPI KQKYGNKISW ADLIIFAGNV 

       190        200        210        220        230        240 
ALESAGFKTF GFAFGRQDIW EPEEILWGQE DTWLGTDKRY GGTNDSTNRE LANPYGATTM 

       250        260        270        280        290        300 
GLIYVNPEGP EGKPDPLAAA HDIRETFGRM AMNDEETAAL IVGGHTLGKT HGPGPGDLVG 

       310        320        330        340        350        360 
PEPEAAPIEQ QGLGWKCAFG SGKGSDTITS GLEVVWTTTP TKWSNSYLEI LYGYEWELTK 

       370        380        390        400        410        420 
SPGDAWQFEA KDAEAIIPDP FGGPPRKPTM LVTDISMRVD PIYGPITRRW LEHPEELNEA 

       430        440        450        460        470        480 
FAKAWYKLLH RDMGPISRYL GPWIPEPQLW QDPVPDVDHP LVDEQDIAAL KEKLLDSGLS 

       490        500        510        520        530        540 
VQQLVKTAWS AAASFRGTDK RGGANGGRLR LQPQRNWEVN EPSELDKALP VLERIAQDFN 

       550        560        570        580        590        600 
ASASDGKKIS LADLIVLGGS AAIEKAARDG GYEVKVHFVA GRTDASQENT DVDSFAVLEP 

       610        620        630        640        650        660 
RADGFRNFVR PGDKAPLEQL LVDKAYFLNL TAPEMTVLVG GLRALNTNHG GSKHGVFTAN 

       670        680        690        700        710        720 
PGALSNDFFV NLLDMSTEWK PSETAENVYE GRDRRTGQTR WTATANDLVF GSNSVLRAVA 

       730        740        750 
EVYAQEDNKA KMIEDFVAAW VKVMNNDRFD LD

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References

[1]	"katGI and katGII encode two different catalases-peroxidases in Mycobacterium fortuitum." Menendez M.C., Ainsa J.A., Martin C., Garcia M.J. J. Bacteriol. 179:6880-6886(1997) [PubMed: 9371430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6841 / DSM 46621 / JCM 6387 / NBRC 13159 / NCTC 10394.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y07865 Genomic DNA. Translation: CAA69192.1.
3D structure databases
ProteinModelPortal	O08404.
SMR	O08404. Positions 44-751.
ModBase	Search...
Protein family/group databases
PeroxiBase	2396. MfoCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_MYCFO

Accession

Primary (citable) accession number: O08404

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 1, 1997
Last modified:	October 19, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families