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Protein

Urease subunit alpha

Gene

ureC

Organism
Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit alpha (ureC), Urease subunit beta (ureB), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi139 – 1391Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi141 – 1411Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi222 – 2221Nickel 1; via carbamate groupUniRule annotation
Metal bindingi222 – 2221Nickel 2; via carbamate groupUniRule annotation
Binding sitei224 – 2241SubstrateUniRule annotation
Metal bindingi251 – 2511Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi277 – 2771Nickel 2; via tele nitrogenUniRule annotation
Active sitei325 – 3251Proton donorUniRule annotation
Metal bindingi365 – 3651Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciBBRO257310:BB4323-MONOMER.
UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:BB4323
OrganismiBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Taxonomic identifieri257310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000001027 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 571571Urease subunit alphaPRO_0000067539Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei222 – 2221N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi257310.BB4323.

Structurei

3D structure databases

ProteinModelPortaliO08400.
SMRiO08400. Positions 2-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 571438UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08400-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRISRSAYA EIYGPTVVGG VGDRVRLADT LLLAEVEKDH TIFGEEVKFG
60 70 80 90 100
GGKVIRDGMG QSQRLATDCV DTVITNALII DAVTGIVKAD IGIKDGLISG
110 120 130 140 150
IGKAGNPDTQ PGVTIIIGAS TEVVAGEGLI VTAGAIDTHI HFICPQQIEE
160 170 180 190 200
ALATGTTTMI GGGTGPATGS LATTSTSGPW HMAAMLQALD TFPVNVGLFG
210 220 230 240 250
KGSSSSHGAL LEQVRAGAMG LKIHEDWAST PASIDTCLNV AEETDIQVAI
260 270 280 290 300
HSDTLNESGF VEDTFAAFKG RTIHSFHTEG AGGGHAPDII RAAGMPNVLP
310 320 330 340 350
ASTNPTMPFT RNTIDEHLDM VMVCHHLDPS IAEDLAFAES RIRRETIAAE
360 370 380 390 400
DILHDLGAFS IMSSDSQAMG RVGEIVLRTW QTAHKMKLQR GPLQGDSERS
410 420 430 440 450
DNERIKRYIA KYTINPAVAH GIAHLVGSVE VGKLADLVLW KPAFFGVKVN
460 470 480 490 500
MVLKSGMAVS ASIGDMGASI STPQPVQIRP MWGSHGKALR TSVAFVSQVS
510 520 530 540 550
LSNPAVSELG LNKRLEAVRG CRGVTKHDMV RNNWLPAISV DPQTYQVYAD
560 570
GQLLRCEALA ELPMAQRYFL F
Length:571
Mass (Da):60,647
Last modified:September 19, 2003 - v2
Checksum:i21B17ABE17F7B416
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti191 – 1911T → A in AAC46128 (PubMed:9524276).Curated
Sequence conflicti555 – 5551R → K in AAC46128 (PubMed:9524276).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000579 Genomic DNA. Translation: AAC46128.1.
BX640450 Genomic DNA. Translation: CAE34686.1.
RefSeqiWP_010927100.1. NC_002927.3.

Genome annotation databases

EnsemblBacteriaiCAE34686; CAE34686; BB4323.
KEGGibbr:BB4323.
PATRICi21142097. VBIBorBro124907_4395.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000579 Genomic DNA. Translation: AAC46128.1.
BX640450 Genomic DNA. Translation: CAE34686.1.
RefSeqiWP_010927100.1. NC_002927.3.

3D structure databases

ProteinModelPortaliO08400.
SMRiO08400. Positions 2-571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257310.BB4323.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE34686; CAE34686; BB4323.
KEGGibbr:BB4323.
PATRICi21142097. VBIBorBro124907_4395.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciBBRO257310:BB4323-MONOMER.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterisation of the urease gene cluster in Bordetella bronchiseptica."
    McMillan D.J., Mau M., Walker M.J.
    Gene 208:243-251(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BB7866.
  2. "Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica."
    Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S.
    , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J.
    Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-588 / NCTC 13252 / RB50.

Entry informationi

Entry nameiURE1_BORBR
AccessioniPrimary (citable) accession number: O08400
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 19, 2003
Last modified: November 11, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.