Urease subunit alpha - Bordetella bronchiseptica

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Reviewed, UniProtKB/Swiss-Prot O08400 (URE1_BORBR)

Last modified November 3, 2009. Version 78. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha

Gene names

Name:	ureC
Ordered Locus Names:	BB4323

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

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Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Catalytic activity	Urea + H₂O = CO₂ + 2 NH₃. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity.
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.

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Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 571

571

Urease subunit alpha HAMAP MF_01953

PRO_0000067539

Regions

Domain

134 – 571

438

Urease

Sites

Active site

325

Proton donor By similarity

Metal binding

139

Nickel 2 By similarity

Metal binding

141

Nickel 2 By similarity

Metal binding

222

Nickel 1; via carbamate group By similarity

Metal binding

222

Nickel 2; via carbamate group By similarity

Metal binding

251

Nickel 1 By similarity

Metal binding

277

Nickel 1 By similarity

Metal binding

365

Nickel 2 By similarity

Binding site

224

Substrate By similarity

Amino acid modifications

Modified residue

222

N6-carboxylysine By similarity

Experimental info

Sequence conflict

191

T → A in AAC46128. Ref.1

Sequence conflict

555

R → K in AAC46128. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

O08400-1 [UniParc].

Last modified September 19, 2003. Version 2.
Checksum: 21B17ABE17F7B416
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FASTA

571

60,647

        10         20         30         40         50         60 
MTRISRSAYA EIYGPTVVGG VGDRVRLADT LLLAEVEKDH TIFGEEVKFG GGKVIRDGMG 

        70         80         90        100        110        120 
QSQRLATDCV DTVITNALII DAVTGIVKAD IGIKDGLISG IGKAGNPDTQ PGVTIIIGAS 

       130        140        150        160        170        180 
TEVVAGEGLI VTAGAIDTHI HFICPQQIEE ALATGTTTMI GGGTGPATGS LATTSTSGPW 

       190        200        210        220        230        240 
HMAAMLQALD TFPVNVGLFG KGSSSSHGAL LEQVRAGAMG LKIHEDWAST PASIDTCLNV 

       250        260        270        280        290        300 
AEETDIQVAI HSDTLNESGF VEDTFAAFKG RTIHSFHTEG AGGGHAPDII RAAGMPNVLP 

       310        320        330        340        350        360 
ASTNPTMPFT RNTIDEHLDM VMVCHHLDPS IAEDLAFAES RIRRETIAAE DILHDLGAFS 

       370        380        390        400        410        420 
IMSSDSQAMG RVGEIVLRTW QTAHKMKLQR GPLQGDSERS DNERIKRYIA KYTINPAVAH 

       430        440        450        460        470        480 
GIAHLVGSVE VGKLADLVLW KPAFFGVKVN MVLKSGMAVS ASIGDMGASI STPQPVQIRP 

       490        500        510        520        530        540 
MWGSHGKALR TSVAFVSQVS LSNPAVSELG LNKRLEAVRG CRGVTKHDMV RNNWLPAISV 

       550        560        570 
DPQTYQVYAD GQLLRCEALA ELPMAQRYFL F

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterisation of the urease gene cluster in Bordetella bronchiseptica." McMillan D.J., Mau M., Walker M.J. Gene 208:243-251(1998) [PubMed: 9524276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BB7866.
[2]	"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

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Cross-references

Sequence databases
	AF000579 Genomic DNA. Translation: AAC46128.1. BX640450 Genomic DNA. Translation: CAE34686.1.
RefSeq	NP_890857.1.
3D structure databases
HSSP	HSSP built from PDB template 1EJX based on UniProtKB P18314.
ModBase	Search...
Protein family/group databases
MEROPS	M38.982.
Genome annotation databases
GeneID	2661104.
GenomeReviews	Gene locus BB4323 in contig BX470250_GR.
KEGG	bbr:BB4323.
NMPDR	fig\|257310.1.peg.4304.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O08400.
OMA	SHIHFIC.
Enzyme and pathway databases
BioCyc	BBRO257310:BB4323-MON.
BRENDA	3.5.1.5. 413.
Family and domain databases
HAMAP	MF_01953. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017952. Urease_asu_core. IPR017950. Urease_asu_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
TIGRFAMs	TIGR01792. urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

URE1_BORBR

Accession

Primary (citable) accession number: O08400

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 19, 2003
Last modified:	November 3, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents