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O08394

- CYPD_BACSU

UniProt

O08394 - CYPD_BACSU

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Protein

Probable bifunctional P-450/NADPH-P450 reductase 1

Gene
cypD, CYP102A2, yetO, yfnJ, BSU07250
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Functions as a fatty acid monooxygenase. The reductase domain is required for electron transfer from NADP to cytochrome P450 By similarity.

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.
RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

FAD By similarity.
FMN By similarity.
Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi403 – 4031Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADPH-hemoprotein reductase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciBSUB:BSU07250-MONOMER.
SABIO-RKO08394.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional P-450/NADPH-P450 reductase 1
Including the following 2 domains:
Cytochrome P450 102 (EC:1.14.14.1)
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Gene namesi
Name:cypD
Synonyms:CYP102A2, yetO, yfnJ
Ordered Locus Names:BSU07250
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU07250. [Micado]

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10611061Probable bifunctional P-450/NADPH-P450 reductase 1PRO_0000052206Add
BLAST

Proteomic databases

PaxDbiO08394.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU07250.

Structurei

3D structure databases

ProteinModelPortaliO08394.
SMRiO08394. Positions 7-454, 476-1059.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini493 – 632140Flavodoxin-likeAdd
BLAST
Domaini671 – 904234FAD-binding FR-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 474474Cytochrome P450Add
BLAST
Regioni475 – 1061587NADPH-P-450 reductaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the cytochrome P450 family.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000093545.
KOiK14338.
OMAiTHEPNWR.
OrthoDBiEOG63Z73K.
PhylomeDBiO08394.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR023206. Bifunctional_P450_P450_red.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF000209. Bifunctional_P450_P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF48264. SSF48264. 1 hit.
SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08394-1 [UniParc]FASTAAdd to Basket

« Hide

MKETSPIPQP KTFGPLGNLP LIDKDKPTLS LIKLAEEQGP IFQIHTPAGT     50
TIVVSGHELV KEVCDEERFD KSIEGALEKV RAFSGDGLFT SWTHEPNWRK 100
AHNILMPTFS QRAMKDYHEK MVDIAVQLIQ KWARLNPNEA VDVPGDMTRL 150
TLDTIGLCGF NYRFNSYYRE TPHPFINSMV RALDEAMHQM QRLDVQDKLM 200
VRTKRQFRYD IQTMFSLVDS IIAERRANGD QDEKDLLARM LNVEDPETGE 250
KLDDENIRFQ IITFLIAGHE TTSGLLSFAT YFLLKHPDKL KKAYEEVDRV 300
LTDAAPTYKQ VLELTYIRMI LNESLRLWPT APAFSLYPKE DTVIGGKFPI 350
TTNDRISVLI PQLHRDRDAW GKDAEEFRPE RFEHQDQVPH HAYKPFGNGQ 400
RACIGMQFAL HEATLVLGMI LKYFTLIDHE NYELDIKQTL TLKPGDFHIS 450
VQSRHQEAIH ADVQAAEKAA PDEQKEKTEA KGASVIGLNN RPLLVLYGSD 500
TGTAEGVARE LADTASLHGV RTKTAPLNDR IGKLPKEGAV VIVTSSYNGK 550
PPSNAGQFVQ WLQEIKPGEL EGVHYAVFGC GDHNWASTYQ YVPRFIDEQL 600
AEKGATRFSA RGEGDVSGDF EGQLDEWKKS MWADAIKAFG LELNENADKE 650
RSTLSLQFVR GLGESPLARS YEASHASIAE NRELQSADSD RSTRHIEIAL 700
PPDVEYQEGD HLGVLPKNSQ TNVSRILHRF GLKGTDQVTL SASGRSAGHL 750
PLGRPVSLHD LLSYSVEVQE AATRAQIREL ASFTVCPPHR RELEELSAEG 800
VYQEQILKKR ISMLDLLEKY EACDMPFERF LELLRPLKPR YYSISSSPRV 850
NPRQASITVG VVRGPAWSGR GEYRGVASND LAERQAGDDV VMFIRTPESR 900
FQLPKDPETP IIMVGPGTGV APFRGFLQAR DVLKREGKTL GEAHLYFGCR 950
NDRDFIYRDE LERFEKDGIV TVHTAFSRKE GMPKTYVQHL MADQADTLIS 1000
ILDRGGRLYV CGDGSKMAPD VEAALQKAYQ AVHGTGEQEA QNWLRHLQDT 1050
GMYAKDVWAG I 1061
Length:1,061
Mass (Da):119,468
Last modified:July 1, 1997 - v1
Checksum:i7915DACC20578978
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87979 Genomic DNA. Translation: BAA20123.1.
AL009126 Genomic DNA. Translation: CAB12544.1.
PIRiD69799.
RefSeqiNP_388606.1. NC_000964.3.
WP_003242884.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB12544; CAB12544; BSU07250.
GeneIDi938784.
KEGGibsu:BSU07250.
PATRICi18973100. VBIBacSub10457_0764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87979 Genomic DNA. Translation: BAA20123.1 .
AL009126 Genomic DNA. Translation: CAB12544.1 .
PIRi D69799.
RefSeqi NP_388606.1. NC_000964.3.
WP_003242884.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali O08394.
SMRi O08394. Positions 7-454, 476-1059.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU07250.

Proteomic databases

PaxDbi O08394.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB12544 ; CAB12544 ; BSU07250 .
GeneIDi 938784.
KEGGi bsu:BSU07250.
PATRICi 18973100. VBIBacSub10457_0764.

Organism-specific databases

GenoListi BSU07250. [Micado ]

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000093545.
KOi K14338.
OMAi THEPNWR.
OrthoDBi EOG63Z73K.
PhylomeDBi O08394.

Enzyme and pathway databases

BioCyci BSUB:BSU07250-MONOMER.
SABIO-RK O08394.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR023206. Bifunctional_P450_P450_red.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00067. p450. 1 hit.
[Graphical view ]
PIRSFi PIRSF000209. Bifunctional_P450_P450R. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF48264. SSF48264. 1 hit.
SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the Bacillus subtilis genome region in the vicinity of the lev operon reveals two new extracytoplasmic function RNA polymerase sigma factors SigV and SigZ."
    Sorokin A., Bolotin A., Purnelle B., Hilbert H., Lauber J., Duesterhoeft A., Ehrlich S.D.
    Microbiology 143:2939-2943(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiCYPD_BACSU
AccessioniPrimary (citable) accession number: O08394
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: September 3, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi