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O08394

- CYPD_BACSU

UniProt

O08394 - CYPD_BACSU

Protein

Probable bifunctional P-450/NADPH-P450 reductase 1

Gene

cypD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Functions as a fatty acid monooxygenase. The reductase domain is required for electron transfer from NADP to cytochrome P450 By similarity.By similarity

    Catalytic activityi

    NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.
    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    FAD.By similarity
    FMN.By similarity
    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi403 – 4031Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. FMN binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. NADPH-hemoprotein reductase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciBSUB:BSU07250-MONOMER.
    SABIO-RKO08394.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable bifunctional P-450/NADPH-P450 reductase 1
    Including the following 2 domains:
    Cytochrome P450 102 (EC:1.14.14.1)
    NADPH--cytochrome P450 reductase (EC:1.6.2.4)
    Gene namesi
    Name:cypD
    Synonyms:CYP102A2, yetO, yfnJ
    Ordered Locus Names:BSU07250
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU07250. [Micado]

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10611061Probable bifunctional P-450/NADPH-P450 reductase 1PRO_0000052206Add
    BLAST

    Proteomic databases

    PaxDbiO08394.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU07250.

    Structurei

    3D structure databases

    ProteinModelPortaliO08394.
    SMRiO08394. Positions 7-454, 476-1059.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini493 – 632140Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini671 – 904234FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 474474Cytochrome P450Add
    BLAST
    Regioni475 – 1061587NADPH-P-450 reductaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the cytochrome P450 family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000093545.
    KOiK14338.
    OMAiTHEPNWR.
    OrthoDBiEOG63Z73K.
    PhylomeDBiO08394.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProiIPR023206. Bifunctional_P450_P450_red.
    IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF00067. p450. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000209. Bifunctional_P450_P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08394-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKETSPIPQP KTFGPLGNLP LIDKDKPTLS LIKLAEEQGP IFQIHTPAGT     50
    TIVVSGHELV KEVCDEERFD KSIEGALEKV RAFSGDGLFT SWTHEPNWRK 100
    AHNILMPTFS QRAMKDYHEK MVDIAVQLIQ KWARLNPNEA VDVPGDMTRL 150
    TLDTIGLCGF NYRFNSYYRE TPHPFINSMV RALDEAMHQM QRLDVQDKLM 200
    VRTKRQFRYD IQTMFSLVDS IIAERRANGD QDEKDLLARM LNVEDPETGE 250
    KLDDENIRFQ IITFLIAGHE TTSGLLSFAT YFLLKHPDKL KKAYEEVDRV 300
    LTDAAPTYKQ VLELTYIRMI LNESLRLWPT APAFSLYPKE DTVIGGKFPI 350
    TTNDRISVLI PQLHRDRDAW GKDAEEFRPE RFEHQDQVPH HAYKPFGNGQ 400
    RACIGMQFAL HEATLVLGMI LKYFTLIDHE NYELDIKQTL TLKPGDFHIS 450
    VQSRHQEAIH ADVQAAEKAA PDEQKEKTEA KGASVIGLNN RPLLVLYGSD 500
    TGTAEGVARE LADTASLHGV RTKTAPLNDR IGKLPKEGAV VIVTSSYNGK 550
    PPSNAGQFVQ WLQEIKPGEL EGVHYAVFGC GDHNWASTYQ YVPRFIDEQL 600
    AEKGATRFSA RGEGDVSGDF EGQLDEWKKS MWADAIKAFG LELNENADKE 650
    RSTLSLQFVR GLGESPLARS YEASHASIAE NRELQSADSD RSTRHIEIAL 700
    PPDVEYQEGD HLGVLPKNSQ TNVSRILHRF GLKGTDQVTL SASGRSAGHL 750
    PLGRPVSLHD LLSYSVEVQE AATRAQIREL ASFTVCPPHR RELEELSAEG 800
    VYQEQILKKR ISMLDLLEKY EACDMPFERF LELLRPLKPR YYSISSSPRV 850
    NPRQASITVG VVRGPAWSGR GEYRGVASND LAERQAGDDV VMFIRTPESR 900
    FQLPKDPETP IIMVGPGTGV APFRGFLQAR DVLKREGKTL GEAHLYFGCR 950
    NDRDFIYRDE LERFEKDGIV TVHTAFSRKE GMPKTYVQHL MADQADTLIS 1000
    ILDRGGRLYV CGDGSKMAPD VEAALQKAYQ AVHGTGEQEA QNWLRHLQDT 1050
    GMYAKDVWAG I 1061
    Length:1,061
    Mass (Da):119,468
    Last modified:July 1, 1997 - v1
    Checksum:i7915DACC20578978
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87979 Genomic DNA. Translation: BAA20123.1.
    AL009126 Genomic DNA. Translation: CAB12544.1.
    PIRiD69799.
    RefSeqiNP_388606.1. NC_000964.3.
    WP_003242884.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12544; CAB12544; BSU07250.
    GeneIDi938784.
    KEGGibsu:BSU07250.
    PATRICi18973100. VBIBacSub10457_0764.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87979 Genomic DNA. Translation: BAA20123.1 .
    AL009126 Genomic DNA. Translation: CAB12544.1 .
    PIRi D69799.
    RefSeqi NP_388606.1. NC_000964.3.
    WP_003242884.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali O08394.
    SMRi O08394. Positions 7-454, 476-1059.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU07250.

    Proteomic databases

    PaxDbi O08394.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12544 ; CAB12544 ; BSU07250 .
    GeneIDi 938784.
    KEGGi bsu:BSU07250.
    PATRICi 18973100. VBIBacSub10457_0764.

    Organism-specific databases

    GenoListi BSU07250. [Micado ]

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000093545.
    KOi K14338.
    OMAi THEPNWR.
    OrthoDBi EOG63Z73K.
    PhylomeDBi O08394.

    Enzyme and pathway databases

    BioCyci BSUB:BSU07250-MONOMER.
    SABIO-RK O08394.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProi IPR023206. Bifunctional_P450_P450_red.
    IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF00067. p450. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000209. Bifunctional_P450_P450R. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the Bacillus subtilis genome region in the vicinity of the lev operon reveals two new extracytoplasmic function RNA polymerase sigma factors SigV and SigZ."
      Sorokin A., Bolotin A., Purnelle B., Hilbert H., Lauber J., Duesterhoeft A., Ehrlich S.D.
      Microbiology 143:2939-2943(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiCYPD_BACSU
    AccessioniPrimary (citable) accession number: O08394
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3