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O08387 (SECY_STAA8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein translocase subunit SecY
Gene names
Name:secY
Ordered Locus Names:SAOUHSC_02491
OrganismStaphylococcus aureus (strain NCTC 8325) [Reference proteome] [HAMAP]
Taxonomic identifier93061 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Staphylococcus ›

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently By similarity. HAMAP-Rule MF_01465

Subunit structure

Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01465.

Sequence similarities

Belongs to the SecY/SEC61-alpha family.

Sequence caution

The sequence AAB54022.1 differs from that shown. Reason: Frameshift at several positions.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 430430Protein translocase subunit SecY HAMAP-Rule MF_01465
PRO_0000131745

Regions

Transmembrane18 – 3821Helical; Potential
Transmembrane68 – 8821Helical; Potential
Transmembrane117 – 13721Helical; Potential
Transmembrane147 – 16721Helical; Potential
Transmembrane179 – 19921Helical; Potential
Transmembrane217 – 23721Helical; Potential
Transmembrane269 – 28921Helical; Potential
Transmembrane308 – 32821Helical; Potential
Transmembrane368 – 38821Helical; Potential
Transmembrane389 – 40921Helical; Potential

Experimental info

Sequence conflict1591A → T in AAB54022. Ref.1
Sequence conflict200 – 2023GQT → YQQ in AAB54022. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O08387 [UniParc].

Last modified July 11, 2006. Version 2.
Checksum: 31B735FEC788F1E4

FASTA43047,148
        10         20         30         40         50         60 
MIQTLVNFFR TKEVRNKIFF TLAMLVIFKI GTYIPAPGVN PAAFDNPQGS QGATELLNTF 

        70         80         90        100        110        120 
GGGALKRFSI FAMGIVPYIT ASIVMQLLQM DIVPKFSEWA KQGEVGRRKL NNVTRYLAIS 

       130        140        150        160        170        180 
LAFIQSIGMA FQFNNYLKGA LIINQSIMSY LLIALVLTAG TAFLIWLGDQ ITQFGVGNGI 

       190        200        210        220        230        240 
SIIIFAGILS TLPASLIQFG QTAFVGQEDT SLAWLKVLGL LVSLILLTVG AIYVLEAVRK 

       250        260        270        280        290        300 
IPIQYAKKQT AQRLGSQATY LPLKVNSAGV IPVIFAMAFF LLPRTLTLFY PDKEWAQNIA 

       310        320        330        340        350        360 
NAANPSSNVG MVVYIVLIIL FTYFYAFVQV NPEKMADNLK KQGSYVPGIR PGEQTKKYIT 

       370        380        390        400        410        420 
KVLYRLTFVG SIFLAVISIL PILATKFMGL PQSIQIGGTS LLIVIGVAIE TMKSLEAQVS 

       430 
QKEYKGFGGR

« Hide

References

« Hide 'large scale' references
[1]Segarra R.A., Iandolo J.J.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCTC 8325.
[2]"The Staphylococcus aureus NCTC8325 genome."
Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 8325.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U96620 Genomic DNA. Translation: AAB54022.1. Frameshift.
CP000253 Genomic DNA. Translation: ABD31510.1.
RefSeqYP_500959.1. NC_007795.1.

3D structure databases

ProteinModelPortalO08387.
SMRO08387. Positions 13-425.
ModBaseSearch...

Protein-protein interaction databases

STRING93061.SAOUHSC_02491.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD31510; ABD31510; SAOUHSC_02491.
GeneID3920868.
KEGGsao:SAOUHSC_02491.
PATRIC19582282. VBIStaAur99865_2247.

Phylogenomic databases

eggNOGCOG0201.
HOGENOMHOG000080586.
KOK03076.
OMAFIMWLGE.
ProtClustDBPRK09204.

Enzyme and pathway databases

BioCycSAUR93061:GIWJ-2427-MONOMER.

Family and domain databases

Gene3D1.10.3370.10. 1 hit.
HAMAPMF_01465. SecY.
InterProIPR026593. SecY.
IPR002208. SecY/SEC61-alpha.
IPR023201. SecY_su_dom.
[Graphical view]
PANTHERPTHR10906. PTHR10906. 1 hit.
PfamPF00344. SecY. 1 hit.
[Graphical view]
PIRSFPIRSF004557. SecY. 1 hit.
SUPFAMSSF103491. SecY. 1 hit.
TIGRFAMsTIGR00967. 3a0501s007. 1 hit.
PROSITEPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSECY_STAA8
AccessionPrimary (citable) accession number: O08387
Secondary accession number(s): Q2FW26
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 11, 2006
Last modified: May 1, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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