O08361 (PUR7_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	ML2227
ORF Names:	MLCB5.16

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137		PRO_0000100842

Sequences

	Sequence		Length	Mass (Da)
	O08361 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 6B3E81E6702FA680	FASTA	297	33,163
	10 20 30 40 50 60 MRPALFDYRH LASGKVREIY RIDDEHLLMV ASDRISAFDY VLDSTIPDKG RILTAMSVFF 70 80 90 100 110 120 FSFVDAPNHL AGPPDDPRIP EEVLGRALVV RQLDMLPMEC VARGYLTGSG LLDYQATGKV 130 140 150 160 170 180 CGIPLPPGLV EASRFAAPLF TPAMKAVLGE HDENISFSRM IEMVGVVRAN QLRDRTLQIY 190 200 210 220 230 240 LQAADHALKT GIIIADTKFE FGIDRDGNLL LADEIFTPDS SRYWSVDDYR VGVVQRSFDK 250 260 270 280 290 QFVRNWLVSP ESGWVRAGAL SPPSLPDDII EATRLCYVEV YERISGMSFD DWIGPGA

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z95151 Genomic DNA. Translation: CAB08411.1. AL583924 Genomic DNA. Translation: CAC31183.1.
PIR	G87187.
RefSeq	NP_302456.1. NC_002677.1.
3D structure databases
ProteinModelPortal	O08361.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000028694; EBMYCP00000028300; EBMYCG00000028689.
GeneID	908227.
GenomeReviews	Gene locus ML2227 in contig AL450380_GR.
KEGG	mle:ML2227.
NMPDR	fig|272631.1.peg.1328.
PATRIC	18058617. VBIMycLep78757_4227.
Organism-specific databases
Leproma	ML2227.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017539.
HOGENOM	HBG555040.
OMA	LTLMDEV.
ProtClustDB	PRK13961.
Enzyme and pathway databases
BioCyc	MLEP272631:ML2227-MONOMER.
Family and domain databases
HAMAP	MF_00137. SAICAR_synth. [Tree]
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synth. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
KO	K01923.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00081. PurC. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR7_MYCLE

Accession

Primary (citable) accession number: O08361

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program