Unreviewed, UniProtKB/TrEMBL O08355 (O08355_PSEFL)

Names and origin

Protein names

Submitted name:
    Mannitol dehydrogenase EMBL AAC04472.1
    EC=1.1.1.67

Gene names

Name:

mtlD EMBL AAC04472.1

Organism

Pseudomonas fluorescens EMBL AAC04472.1

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Ontologies

Keywords
Ligand	NAD Spearmint SPM013118
Molecular function	Oxidoreductase Spearmint SPM013118
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: InterPro
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro mannitol 2-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O08355-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 7C12DFA443CEA443	FASTA	493	54,498
	10 20 30 40 50 60 MKLNKQNLTQ LAPEVKLPAY TLADTRQGIA HIGVGGFHRA HQAYYTDALM NTGEGLDWSI 70 80 90 100 110 120 CGVGLRSEDR KARDDLAGQD YLFTLYELGD TDDTEVRVIG SISDMLLAED SAQALIDKLA 130 140 150 160 170 180 SPEIRIVSLT ITEGGYCIDD SNGEFMAHLP QIQHDLAHPS SPKTVFGFIC AALTQRRAAG 190 200 210 220 230 240 IPAFTVMSCD NLPHNGAVTR KALLAFAALH NAELHDWIKA HVSFPNAMVD RITPMTSTAH 250 260 270 280 290 300 RLQLHDEHGI DDAWPVVCEP FVQWVLEDKF VNGRPAWEKV GVQFTDDVTP YEEMKIGLLN 310 320 330 340 350 360 GSHLALTYLG FLKGYRFVHE TMNDPLFVAY MRAYMDLDVT PNLAPVPGID LTDYKQTLVD 370 380 390 400 410 420 RFSNQAIADQ LERVCSDGSS KFPKFTVPTI NRLIADGRET ERAALVVAAW ALYLKGVDEN 430 440 450 460 470 480 GVSYTIPDPR AEFCQGLVSD DALISQRLLA VEEIFGTAIP NSPEFVAAFE RCYGSLRDNG 490 VTTTLKHLLK KPV

References

[1]	"Cloning, nucleotide sequence and expression of a mannitol dehydrogenase gene from Pseudomonas fluorescens DSM 50106 in Escherichia coli." Brunker P., Altenbuchner J., Kulbe K.D., Mattes R. Biochim. Biophys. Acta 1351:157-167(1997) [PubMed: 9116029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM50106 EMBL AAC04472.1.
[2]	"Structure and function of the genes involved in mannitol, arabitol and glucitol utilization from Pseudomonas fluorescens DSM50106." Brunker P., Altenbuchner J., Mattes R. Gene 206:117-126(1998) [PubMed: 9461423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM50106 EMBL AAC04472.1.
[3]	Bruenker P., Altenbuchner J., Mattes R. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM50106 EMBL AAC04472.1.
[4]	"Crystal structure of Pseudomonas fluorescens mannitol 2-dehydrogenase binary and ternary complexes. Specificity and catalytic mechanism." Kavanagh K.L., Klimacek M., Nidetzky B., Wilson D.K. J. Biol. Chem. 277:43433-43442(2002) [PubMed: 12196534] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF007800 Genomic DNA. Translation: AAC04472.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LJ8	X-ray	1.70	A	1-493	[»]
1M2W	X-ray	1.80	A/B	1-493	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR013328. DH_multihelical.
IPR013118. Mannitol_DH_C.
IPR000669. Mannitol_DH_core.
IPR013131. Mannitol_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:1.10.1040.10. Opine_DH. 1 hit.

Pfam

PF01232. Mannitol_dh. 1 hit.
PF08125. Mannitol_dh_C. 1 hit.
[Graphical view]

PRINTS

PR00084. MTLDHDRGNASE.

PROSITE

PS00974. MANNITOL_DHGENASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

O08355_PSEFL

Accession

Primary (citable) accession number: O08355

Entry history

Integrated into UniProtKB/TrEMBL:	July 1, 1997
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)