Reviewed, UniProtKB/Swiss-Prot O08353 (ILVB_METAO)

Names and origin

Protein names

Recommended name:
    Probable acetolactate synthase large subunit
      Short name=AHAS
    EC=2.2.1.6
Alternative name(s):
    Acetohydroxy-acid synthase large subunit
      Short name=ALS

Gene names

Name:

ilvB

Organism

Methanococcus aeolicus

Taxonomic identifier

42879 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of large and small chains By similarity.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acetolactate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	Probable acetolactate synthase large subunit		PRO_0000090807
Regions
	Nucleotide binding	258 – 279	22	FAD By similarity
	Nucleotide binding	301 – 320	20	FAD By similarity
	Region	404 – 484	81	Thiamine pyrophosphate binding
Sites
	Metal binding	455	1	Magnesium By similarity
	Metal binding	482	1	Magnesium By similarity
	Binding site	47	1	Thiamine pyrophosphate By similarity
	Binding site	149	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08353-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 59630C57DECE0D62	FASTA	599	65,424
	10 20 30 40 50 60 MNGAEAMIKA LEAEKVEILF GYPGGALLPF YDALHHSDLI HLLTRHEQAA AHAADGYARA 70 80 90 100 110 120 SGKVGVCIGT SGPGATNLVT GVATAHSDSS PMVALTGQVP TKLIGNDAFQ EIDALGLFMP 130 140 150 160 170 180 IVKHNFQIQK TCQIPEIFRS AFEIAQTGRP GPVHIDLPKD VQELELDIDK HPIPSKVKLI 190 200 210 220 230 240 GYNPTTIGHP RQIKKAIKLI ASAKRPIILA GGGVLLSGAN EELLKLVELL NIPVCTTLMG 250 260 270 280 290 300 KGCISENHPL ALGMVGMHGT KPANYCLSES DVLISIGCRF SDRITGDIKS FATNAKIIHI 310 320 330 340 350 360 DIDPAEIGKN VNVDVPIVGD AKLILKEVIK QLDYIINKDS KENNDKENIS QWIENVNSLK 370 380 390 400 410 420 KSSIPVMDYD DIPIKPQKIV KELMAVIDDL NINKNTIITT DVGQNQMWMA HYFKTQTPRS 430 440 450 460 470 480 FLSSGGLGTM GFGFPSAIGA KVAKPDSKVI CITGDGGFMM NCQELGTIAE YNIPVVICIF 490 500 510 520 530 540 DNRTLGMVYQ WQNLFYGKRQ CSVNFGGAPD FIKLAESYGI KARRIESPNE INEALKEAIN 550 560 570 580 590 CDEPYLLDFA IDPSSALSMV PPGAKLTNII DAVQEHPNEK IVCFDEIKRR YMENKRNRK

References

[1]	"Cloning and phylogenetic analysis of the genes encoding acetohydroxyacid synthase from the archaeon Methanococcus aeolicus." Bowen T.L., Union J., Tumbula D.L., Whitman W.B. Gene 188:77-84(1997) [PubMed: 9099862] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U35458 Genomic DNA. Translation: AAB53488.1.
3D structure databases
HSSP	HSSP built from PDB template 1N0H based on UniProtKB P07342.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11900.
BRENDA	2.2.1.6. 20955.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVB_METAO

Accession

Primary (citable) accession number: O08353

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)