Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O08349

- MDH_ARCFU

UniProt

O08349 - MDH_ARCFU

Protein

Malate dehydrogenase

Gene

mdh

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321NAD1 Publication
    Binding sitei81 – 811SubstrateBy similarity
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei94 – 941NAD1 Publication
    Binding sitei119 – 1191SubstrateBy similarity
    Binding sitei150 – 1501SubstrateBy similarity
    Active sitei172 – 1721Proton acceptor

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 126NAD1 Publication
    Nucleotide bindingi117 – 1193NAD1 Publication

    GO - Molecular functioni

    1. L-lactate dehydrogenase activity Source: InterPro
    2. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciAFUL224325:GJBC-873-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37)
    Gene namesi
    Name:mdh
    Ordered Locus Names:AF_0855
    OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
    Taxonomic identifieri224325 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
    ProteomesiUP000002199: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 294294Malate dehydrogenasePRO_0000113480Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi224325.AF0855.

    Structurei

    Secondary structure

    1
    294
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65
    Helixi10 – 2213
    Beta strandi26 – 316
    Helixi35 – 4915
    Helixi50 – 523
    Beta strandi57 – 626
    Helixi64 – 674
    Beta strandi71 – 755
    Beta strandi83 – 853
    Helixi87 – 10620
    Beta strandi113 – 1164
    Beta strandi118 – 1203
    Helixi121 – 13111
    Beta strandi138 – 1414
    Helixi144 – 15714
    Beta strandi161 – 1633
    Beta strandi168 – 1703
    Helixi180 – 1823
    Beta strandi185 – 1873
    Helixi191 – 1999
    Helixi201 – 2099
    Helixi214 – 22815
    Beta strandi234 – 24310
    Helixi244 – 2463
    Beta strandi248 – 25912
    Beta strandi262 – 2654
    Helixi272 – 29019
    Turni291 – 2933

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2X0IX-ray2.90A1-294[»]
    2X0JX-ray2.79A1-294[»]
    ProteinModelPortaliO08349.
    SMRiO08349. Positions 1-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO08349.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    KOiK00024.
    OMAiYAPSAFV.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_00487. Malate_dehydrog_3.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR018177. L-lactate_DH_AS.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O08349-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLGFVGAGR VGSTSAFTCL LNLDVDEIAL VDIAEDLAVG EAMDLAHAAA    50
    GIDKYPKIVG GADYSLLKGS EIIVVTAGLA RKPGMTRLDL AHKNAGIIKD 100
    IAKKIVENAP ESKILVVTNP MDVMTYIMWK ESGKPRNEVF GMGNQLDSQR 150
    LKERLYNAGA RNIRRAWIIG EHGDSMFVAK SLADFDGEVD WEAVENDVRF 200
    VAAEVIKRKG ATIFGPAVAI YRMVKAVVED TGEIIPTSMI LQGEYGIENV 250
    AVGVPAKLGK NGAEVADIKL SDEEIEKLRN SAKILRERLE ELGY 294
    Length:294
    Mass (Da):31,874
    Last modified:July 1, 1997 - v1
    Checksum:i90389DBC189B944F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z85985 Genomic DNA. Translation: CAB06654.1.
    AE000782 Genomic DNA. Translation: AAB90384.1.
    PIRiG69356.
    RefSeqiNP_069689.1. NC_000917.1.
    WP_010878358.1. NC_000917.1.

    Genome annotation databases

    EnsemblBacteriaiAAB90384; AAB90384; AF_0855.
    GeneIDi1484074.
    KEGGiafu:AF0855.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z85985 Genomic DNA. Translation: CAB06654.1 .
    AE000782 Genomic DNA. Translation: AAB90384.1 .
    PIRi G69356.
    RefSeqi NP_069689.1. NC_000917.1.
    WP_010878358.1. NC_000917.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2X0I X-ray 2.90 A 1-294 [» ]
    2X0J X-ray 2.79 A 1-294 [» ]
    ProteinModelPortali O08349.
    SMRi O08349. Positions 1-294.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224325.AF0855.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB90384 ; AAB90384 ; AF_0855 .
    GeneIDi 1484074.
    KEGGi afu:AF0855.

    Phylogenomic databases

    eggNOGi COG0039.
    KOi K00024.
    OMAi YAPSAFV.

    Enzyme and pathway databases

    BioCyci AFUL224325:GJBC-873-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O08349.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_00487. Malate_dehydrog_3.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR018177. L-lactate_DH_AS.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Properties and primary structure of a thermostable L-malate dehydrogenase from Archaeoglobus fulgidus."
      Langelandsvik A.S., Steen I.H., Birkeland N.K., Lien T.
      Arch. Microbiol. 168:59-67(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
    2. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
      Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
      , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
      Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
    3. "The 2.9A resolution crystal structure of malate dehydrogenase from Archaeoglobus fulgidus: mechanisms of oligomerisation and thermal stabilisation."
      Irimia A., Vellieux F.M.D., Madern D., Zaccai G., Karshikoff A., Tibbelin G., Ladenstein R., Lien T., Birkeland N.-K.
      J. Mol. Biol. 335:343-356(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.79 ANGSTROMS) IN COMPLEX WITH NAD, SUBUNIT.

    Entry informationi

    Entry nameiMDH_ARCFU
    AccessioniPrimary (citable) accession number: O08349
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3