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Protein

Putative glutamate synthase [NADPH] small chain

Gene

gltD

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Pathwayi: L-glutamate biosynthesis via GLT pathway

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+) route).
Proteins known to be involved in this subpathway in this organism are:
  1. Putative glutamate synthase [NADPH] small chain (gltD)
This subpathway is part of the pathway L-glutamate biosynthesis via GLT pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+) route), the pathway L-glutamate biosynthesis via GLT pathway and in Amino-acid biosynthesis.

Pathwayi: nitrogen metabolism

This protein is involved in the pathway nitrogen metabolism, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrogen metabolism and in Energy metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi33 – 331Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi37 – 371Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi43 – 431Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)Sequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Glutamate biosynthesis

Keywords - Ligandi

Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00045.
UPA00634; UER00689.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glutamate synthase [NADPH] small chain (EC:1.4.1.13)
Alternative name(s):
Glutamate synthase subunit beta
Short name:
GLTS beta chain
NADPH-GOGAT
Gene namesi
Name:gltD
OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifieri1063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Putative glutamate synthase [NADPH] small chainPRO_0000386547Add
BLAST

Proteomic databases

PRIDEiO08340.

Interactioni

Subunit structurei

Aggregate of 4 catalytic active heterodimers, consisting of a large and a small subunit.By similarity

Structurei

3D structure databases

ProteinModelPortaliO08340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
3.50.50.60. 1 hit.
InterProiIPR028261. DPD_II.
IPR023753. FAD/NAD-binding_dom.
IPR009051. Helical_ferredxn.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF14691. Fer4_20. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.

Sequencei

Sequence statusi: Complete.

O08340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEPMLQFV KMARETPEKR PRSLRSQDFH EICGRICPQD RLCEGNCVIE
60 70 80 90 100
QSGHGTVTIG AVEKYITDTA WENGWVVPGK PAYERSESVG IIGAGPGGLA
110 120 130 140 150
AADALRRAGL QVTVYDRYDR AGGLLTYGIP GFKLEKDVVA RRVEQLEQAG
160 170 180 190 200
VQFVLNCNVG EDLSFDAIRG QHDAVLIATG VYKQRDLAAP GVGSAGVVQA
210 220 230 240 250
LSYLTASNRR SFGDEVDDDG LDASGKRVVV IGGGDTAMDC VRTAIRQGAT
260 270 280 290 300
SVKCLYRRDR ANMPGSQREV ANAEEEGVEF VWLSAPRGFI AGDAVEGVIV
310 320 330 340 350
QKMRLGEPDA TGRQMPEIIE GADYVEPADL AIMALGFEPE DLPTLWGVPD
360 370 380 390 400
LTVTRWGTIK ADFRTHATSL PGVYAVGDIV RGASLVVWAI RDGRDAAQSI
410
LDYLAQPAVV AAE
Length:413
Mass (Da):44,407
Last modified:July 1, 1997 - v1
Checksum:iA177838E418A2254
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12481 Genomic DNA. Translation: CAA73084.1.
PIRiJE0142.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12481 Genomic DNA. Translation: CAA73084.1.
PIRiJE0142.

3D structure databases

ProteinModelPortaliO08340.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO08340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00045.
UPA00634; UER00689.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
3.50.50.60. 1 hit.
InterProiIPR028261. DPD_II.
IPR023753. FAD/NAD-binding_dom.
IPR009051. Helical_ferredxn.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF14691. Fer4_20. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The nucleotide Sequence of gltD gene encoding the small subunit of Rhodobacter sphaeroides glutamate synthase."
    Lu T., Wu Y.Q., Song H.Y.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 29:294-302(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 601.

Entry informationi

Entry nameiGLTD_RHOSH
AccessioniPrimary (citable) accession number: O08340
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: July 1, 1997
Last modified: December 9, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Glutamine binds to the large subunit and transfers the amido group to 2-oxo-glutamate that apparently binds to the small subunit.By similarity

Caution

Lacks the 4Fe-4S ferredoxin-type domain found in GltD orthologs.Curated

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.