O08337 (BLA8_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase SHV-8
EC=3.5.2.6

Gene names

Name:	bla
Synonyms:	shv8

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	SHV enzymes hydrolyze broad spectrum cephalosporins notably cefotaxime and ceftazidime.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 286	265	Beta-lactamase SHV-8		PRO_0000016986
Regions
	Region	230 – 232	3	Substrate binding By similarity
Sites
	Active site	66	1	Acyl-ester intermediate By similarity
	Active site	164	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 119		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08337 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: F159990BC8BF5504	FASTA	286	31,223
	10 20 30 40 50 60 MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE 70 80 90 100 110 120 RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 130 140 150 160 170 180 AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARNTTTPA 190 200 210 220 230 240 SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG 250 260 270 280 IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR

References

[1]

"Evolution of extended-spectrum beta-lactam resistance (SHV-8) in a strain of Escherichia coli during multiple episodes of bacteremia." Rasheed J.K., Jay C., Metchock B., Berkowitz F., Weigel L., Crellin J., Steward C., Hill B., Medeiros A.A., Tenover F.C. Antimicrob. Agents Chemother. 41:647-653(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U92041 Genomic DNA. Translation: AAB51384.1.
3D structure databases
ProteinModelPortal	O08337.
SMR	O08337. Positions 22-286.
ModBase	Search...
Proteomic databases
PRIDE	O08337.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA8_ECOLX

Accession

Primary (citable) accession number: O08337

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program