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O08337

- BLA8_ECOLX

UniProt

O08337 - BLA8_ECOLX

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Protein

Beta-lactamase SHV-8

Gene

bla

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

SHV enzymes hydrolyze broad spectrum cephalosporins notably cefotaxime and ceftazidime.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei164 – 1641Proton acceptorBy similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase SHV-8 (EC:3.5.2.6)
Gene namesi
Name:bla
Synonyms:shv8
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 286265Beta-lactamase SHV-8PRO_0000016986Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi73 ↔ 119By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO08337.

Structurei

3D structure databases

ProteinModelPortaliO08337.
SMRiO08337. Positions 22-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni230 – 2323Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08337-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG
60 70 80 90 100
RTLTAWRADE RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD
110 120 130 140 150
YSPVSEKHLA DGMTVGELCA AAITMSDNSA ANLLLATVGG PAGLTAFLRQ
160 170 180 190 200
IGDNVTRLDR WETELNEALP GDARNTTTPA SMAATLRKLL TSQRLSARSQ
210 220 230 240 250
RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG IVALLGPNNK
260 270 280
AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR
Length:286
Mass (Da):31,223
Last modified:July 1, 1997 - v1
Checksum:iF159990BC8BF5504
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U92041 Genomic DNA. Translation: AAB51384.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U92041 Genomic DNA. Translation: AAB51384.1 .

3D structure databases

ProteinModelPortali O08337.
SMRi O08337. Positions 22-286.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O08337.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evolution of extended-spectrum beta-lactam resistance (SHV-8) in a strain of Escherichia coli during multiple episodes of bacteremia."
    Rasheed J.K., Jay C., Metchock B., Berkowitz F., Weigel L., Crellin J., Steward C., Hill B., Medeiros A.A., Tenover F.C.
    Antimicrob. Agents Chemother. 41:647-653(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiBLA8_ECOLX
AccessioniPrimary (citable) accession number: O08337
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: October 1, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3