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O08333

- PFP_STRCO

UniProt

O08333 - PFP_STRCO

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Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene
pfkA1, pfk1, pfkA, pfp, SCO2119, SC6E10.13c
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions By similarity.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Non-allosteric By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Diphosphate; via amide nitrogen By similarity
Metal bindingi103 – 1031Magnesium; catalytic By similarity
Sitei104 – 1041Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP By similarity
Sitei125 – 1251Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi By similarity
Active sitei128 – 1281Proton acceptor By similarity
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarity
Binding sitei222 – 2221Substrate By similarity
Binding sitei266 – 2661Substrate; shared with dimeric partner By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. diphosphate-fructose-6-phosphate 1-phosphotransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (EC:2.7.1.90)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependent
PPi-dependent phosphofructokinase
Short name:
PPi-PFK
Pyrophosphate-dependent 6-phosphofructose-1-kinase
Gene namesi
Name:pfkA1
Synonyms:pfk1, pfkA, pfp
Ordered Locus Names:SCO2119
ORF Names:SC6E10.13c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No effect.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotationPRO_0000111986Add
BLAST

PTM databases

PhosSiteiP1007943.

Interactioni

Subunit structurei

Homodimer or homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO2119.

Structurei

3D structure databases

ProteinModelPortaliO08333.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate binding By similarity
Regioni170 – 1723Substrate binding By similarity
Regioni272 – 2754Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiHRHRAHA.
OrthoDBiEOG644ZRM.
PhylomeDBiO08333.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08333-1 [UniParc]FASTAAdd to Basket

« Hide

MKVGVLTGGG DCPGLNAVIR AVVRKGVQEY GYDFTGFRDG WRGPLEGDTV    50
PLDIPAVRGI LPRGGTVLGS SRTNPLKQRD GIRRIKDNLA ALGVEALITI 100
GGEDTLGVAT RLADEYGVPC VGVPKTIDND LSATDYTFGF DTAVGIATEA 150
IDRLHTTAES HMRVLVVEVM GRHAGWIALH SGLAGGANVI LIPEQRFDVE 200
QVCSWVTSRF RASYAPIVVV AEGAMPRDGD MVLKDESLDS YGHVRLSGVG 250
EWLAKQIEKR TGNEARTTVL GHVQRGGTPS AFDRWLATRF GLHAVDCVHD 300
GDFGKMVALR GTDIVRVPIA EATARLKTVD PALYEEVGVF FG 342
Length:342
Mass (Da):36,664
Last modified:July 1, 1997 - v1
Checksum:iCEEFC7B74092AB34
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51728 Genomic DNA. Translation: AAC45135.1.
AL939111 Genomic DNA. Translation: CAB51967.1.
PIRiT35500.
RefSeqiNP_626376.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB51967; CAB51967; CAB51967.
GeneIDi1097553.
KEGGisco:SCO2119.
PATRICi23733890. VBIStrCoe124346_2154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51728 Genomic DNA. Translation: AAC45135.1 .
AL939111 Genomic DNA. Translation: CAB51967.1 .
PIRi T35500.
RefSeqi NP_626376.1. NC_003888.3.

3D structure databases

ProteinModelPortali O08333.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO2119.

PTM databases

PhosSitei P1007943.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB51967 ; CAB51967 ; CAB51967 .
GeneIDi 1097553.
KEGGi sco:SCO2119.
PATRICi 23733890. VBIStrCoe124346_2154.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248869.
KOi K00850.
OMAi HRHRAHA.
OrthoDBi EOG644ZRM.
PhylomeDBi O08333.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of ATP-dependent phosphofructokinase as a regulatory step in the glycolytic pathway of the actinomycete Streptomyces coelicolor A3(2)."
    Alves A.M.C.R., Euverink G.J.W., Bibb M.J., Dijkhuizen L.
    Appl. Environ. Microbiol. 63:956-961(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A3(2) / 1109.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "Antibiotic overproduction in Streptomyces coelicolor A3(2) mediated by phosphofructokinase deletion."
    Borodina I., Siebring J., Zhang J., Smith C.P., van Keulen G., Dijkhuizen L., Nielsen J.
    J. Biol. Chem. 283:25186-25199(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPFP_STRCO
AccessioniPrimary (citable) accession number: O08333
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally characterized as ATP-dependent phosphofructokinase (1 Publication). However, inspection of the original N-terminal sequence showed that the characterized enzyme was not pfkA1 (SCO2119), but pfkA2 (SCO5426) instead, another isozyme in S.coelicolor (1 Publication).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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