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O08333

- PFP_STRCO

UniProt

O08333 - PFP_STRCO

Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfkA1

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

    Catalytic activityi

    Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Non-allosteric.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101Diphosphate; via amide nitrogenUniRule annotation
    Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
    Sitei104 – 1041Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATPUniRule annotation
    Sitei125 – 1251Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation
    Active sitei128 – 1281Proton acceptorUniRule annotation
    Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei222 – 2221SubstrateUniRule annotation
    Binding sitei266 – 2661Substrate; shared with dimeric partnerUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: InterPro
    2. ATP binding Source: InterPro
    3. diphosphate-fructose-6-phosphate 1-phosphotransferase activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
    Alternative name(s):
    6-phosphofructokinase, pyrophosphate dependentUniRule annotation
    PPi-dependent phosphofructokinaseUniRule annotation
    Short name:
    PPi-PFKUniRule annotation
    Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
    Gene namesi
    Name:pfkA1UniRule annotation
    Synonyms:pfk1, pfkA, pfp
    Ordered Locus Names:SCO2119
    ORF Names:SC6E10.13c
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    No effect.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 342342Pyrophosphate--fructose 6-phosphate 1-phosphotransferasePRO_0000111986Add
    BLAST

    PTM databases

    PhosSiteiP1007943.

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi100226.SCO2119.

    Structurei

    3D structure databases

    ProteinModelPortaliO08333.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni126 – 1283Substrate bindingUniRule annotation
    Regioni170 – 1723Substrate bindingUniRule annotation
    Regioni272 – 2754Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248869.
    KOiK00850.
    OMAiHRHRAHA.
    OrthoDBiEOG644ZRM.
    PhylomeDBiO08333.

    Family and domain databases

    HAMAPiMF_01976. Phosphofructokinase_III.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012829. PFK.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08333-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVGVLTGGG DCPGLNAVIR AVVRKGVQEY GYDFTGFRDG WRGPLEGDTV    50
    PLDIPAVRGI LPRGGTVLGS SRTNPLKQRD GIRRIKDNLA ALGVEALITI 100
    GGEDTLGVAT RLADEYGVPC VGVPKTIDND LSATDYTFGF DTAVGIATEA 150
    IDRLHTTAES HMRVLVVEVM GRHAGWIALH SGLAGGANVI LIPEQRFDVE 200
    QVCSWVTSRF RASYAPIVVV AEGAMPRDGD MVLKDESLDS YGHVRLSGVG 250
    EWLAKQIEKR TGNEARTTVL GHVQRGGTPS AFDRWLATRF GLHAVDCVHD 300
    GDFGKMVALR GTDIVRVPIA EATARLKTVD PALYEEVGVF FG 342
    Length:342
    Mass (Da):36,664
    Last modified:July 1, 1997 - v1
    Checksum:iCEEFC7B74092AB34
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51728 Genomic DNA. Translation: AAC45135.1.
    AL939111 Genomic DNA. Translation: CAB51967.1.
    PIRiT35500.
    RefSeqiNP_626376.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB51967; CAB51967; CAB51967.
    GeneIDi1097553.
    KEGGisco:SCO2119.
    PATRICi23733890. VBIStrCoe124346_2154.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51728 Genomic DNA. Translation: AAC45135.1 .
    AL939111 Genomic DNA. Translation: CAB51967.1 .
    PIRi T35500.
    RefSeqi NP_626376.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali O08333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO2119.

    PTM databases

    PhosSitei P1007943.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB51967 ; CAB51967 ; CAB51967 .
    GeneIDi 1097553.
    KEGGi sco:SCO2119.
    PATRICi 23733890. VBIStrCoe124346_2154.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248869.
    KOi K00850.
    OMAi HRHRAHA.
    OrthoDBi EOG644ZRM.
    PhylomeDBi O08333.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_01976. Phosphofructokinase_III.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012829. PFK.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of ATP-dependent phosphofructokinase as a regulatory step in the glycolytic pathway of the actinomycete Streptomyces coelicolor A3(2)."
      Alves A.M.C.R., Euverink G.J.W., Bibb M.J., Dijkhuizen L.
      Appl. Environ. Microbiol. 63:956-961(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A3(2) / 1109.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.
    3. "Antibiotic overproduction in Streptomyces coelicolor A3(2) mediated by phosphofructokinase deletion."
      Borodina I., Siebring J., Zhang J., Smith C.P., van Keulen G., Dijkhuizen L., Nielsen J.
      J. Biol. Chem. 283:25186-25199(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPFP_STRCO
    AccessioniPrimary (citable) accession number: O08333
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally characterized as ATP-dependent phosphofructokinase (PubMed:9055413). However, inspection of the original N-terminal sequence showed that the characterized enzyme was not pfkA1 (SCO2119), but pfkA2 (SCO5426) instead, another isozyme in S.coelicolor (PubMed:18606812).2 Publications

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3