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O08325 (XYLA_BACMD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Xylose isomerase
EC=5.3.1.5
Gene names
Name:xylA
Ordered Locus Names:BMD_1858
OrganismBacillus megaterium (strain DSM 319) [Complete proteome] [HAMAP]
Taxonomic identifier592022 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-xylose = D-xylulose. HAMAP MF_00455

Cofactor

Binds 2 magnesium ions per subunit By similarity. HAMAP MF_00455

Subunit structure

Homotetramer By similarity. HAMAP MF_00455

Subcellular location

Cytoplasm HAMAP MF_00455.

Sequence similarities

Belongs to the xylose isomerase family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Pentose shunt
Xylose metabolism
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processD-xylose metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

pentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

xylose isomerase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445Xylose isomerase HAMAP MF_00455
PRO_0000195764

Sites

Active site1071 By similarity
Active site1101 By similarity
Metal binding2381Magnesium 1 By similarity
Metal binding2741Magnesium 1 By similarity
Metal binding2741Magnesium 2 By similarity
Metal binding2771Magnesium 2 By similarity
Metal binding3021Magnesium 1 By similarity
Metal binding3131Magnesium 2 By similarity
Metal binding3151Magnesium 2 By similarity
Metal binding3451Magnesium 1 By similarity

Experimental info

Sequence conflict581T → Q in CAA40821. Ref.1
Sequence conflict581T → Q in CAA96094. Ref.2
Sequence conflict1701A → R in CAA40821. Ref.1
Sequence conflict1701A → R in CAA96094. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O08325 [UniParc].

Last modified August 10, 2010. Version 2.
Checksum: 39CB7340E7403622

FASTA44550,167
        10         20         30         40         50         60 
MVQTSTNKIN HFESANKVLY EGKDSKNPLA FKYYNPEEVV GGKTMKDQLR FSVAYWHTFT 

        70         80         90        100        110        120 
ADGTDPFGAA TMQRSWDRYD GMDLAKARVE AAFQLFETLN VPFFAFHDRD IAPEGSTLQE 

       130        140        150        160        170        180 
TNKNLDVIVT MIKEYMQTSN VKLLWNTANM FTNPRFVHGA ATSCNADVFA YAAAQVKKGL 

       190        200        210        220        230        240 
ETAKELGAEN YVFWGGREGY ETLLNTNLQL ELDNLARFMH MAVDYATEIG YTGQFLIEPK 

       250        260        270        280        290        300 
PKEPTTHQYD TDAATTISFL RQYGLDKYFK LNLEANHATL AGHTFEHELR VARVQGLLGS 

       310        320        330        340        350        360 
VDANQGDPLL GWDTDEFPTD LYSTTLAMYE ILQNGGLGSG GLNFDAKVRR GSFEQDDLLY 

       370        380        390        400        410        420 
AHVAGMDAFA RGLKVAHKLV EDRVFENVIN ERYSSFKEGI GLEIVEGKAN FHTLEQYAFK 

       430        440 
NPNIANKSGR QERLKSILNQ YILEV

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization."
Rygus T., Scheler A., Allmansberger R., Hillen W.
Arch. Microbiol. 155:535-542(1991) [PubMed: 1719948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Regulation of expression, genetic organization and substrate specificity of xylose uptake in Bacillus megaterium."
Schmiedel D., Kintrup M., Kuster E., Hillen W.
Mol. Microbiol. 23:1053-1062(1997) [PubMed: 9076741] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 319.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X57598 Genomic DNA. Translation: CAA40821.1.
Z71474 Genomic DNA. Translation: CAA96094.1.
CP001982 Genomic DNA. Translation: ADF38711.1.
RefSeqYP_003597061.1. NC_014103.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000421601; EBBACP00000415988; EBBACG00000419779.
GeneID9117249.
GenomeReviewsGene locus BMD_1858 in contig CP001982_GR.
KEGGbmd:BMD_1858.
PATRIC37253748. VBIBacMeg104484_1800.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000002648.
ProtClustDBPRK05474.

Family and domain databases

HAMAPMF_00455. Xylose_isom_A.
[Tree]
InterProIPR013022. Xyl_isomerase-like_TIM-brl.
IPR012307. Xyl_isomerase_TIM-brl.
IPR013452. Xylose_isom_bac.
IPR001998. Xylose_isomerase.
[Graphical view]
Gene3DG3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
KOK01805.
PfamPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
PRINTSPR00688. XYLOSISMRASE.
SUPFAMSSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.
TIGRFAMsTIGR02630. Xylose_isom_A. 1 hit.
PROSITEPS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXYLA_BACMD
AccessionPrimary (citable) accession number: O08325
Secondary accession number(s): D5DF38
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 10, 2010
Last modified: January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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