Xylose isomerase - Bacillus megaterium

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Reviewed, UniProtKB/Swiss-Prot O08325 (XYLA_BACME)

Last modified September 22, 2009. Version 60. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Xylose isomerase
EC=5.3.1.5

Gene names

Name:

xylA

Organism

Bacillus megaterium

Taxonomic identifier

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-xylose = D-xylulose. HAMAP MF_00455
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00455
Sequence similarities	Belongs to the xylose isomerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Pentose shunt Xylose metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP xylose isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

445

Xylose isomerase HAMAP MF_00455

PRO_0000195764

Sites

Active site

1

By similarity

Active site

1

By similarity

Metal binding

1

Magnesium 1 By similarity

Metal binding

1

Magnesium 1 By similarity

Metal binding

1

Magnesium 2 By similarity

Metal binding

1

Magnesium 2 By similarity

Metal binding

1

Magnesium 1 By similarity

Metal binding

1

Magnesium 2 By similarity

Metal binding

1

Magnesium 2 By similarity

Metal binding

1

Magnesium 1 By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O08325-1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 053A45A2B6B2C72E
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445

50,279

        10         20         30         40         50         60 
MVQTSTNKIN HFESANKVLY EGKDSKNPLA FKYYNPEEVV GGKTMKDQLR FSVAYWHQFT 

        70         80         90        100        110        120 
ADGTDPFGAA TMQRSWDRYD GMDLAKARVE AAFQLFETLN VPFFAFHDRD IAPEGSTLQE 

       130        140        150        160        170        180 
TNKNLDVIVT MIKEYMQTSN VKLLWNTANM FTNPRFVHGA ATSCNADVFR YAAAQVKKGL 

       190        200        210        220        230        240 
ETAKELGAEN YVFWGGREGY ETLLNTNLQL ELDNLARFMH MAVDYATEIG YTGQFLIEPK 

       250        260        270        280        290        300 
PKEPTTHQYD TDAATTISFL RQYGLDKYFK LNLEANHATL AGHTFEHELR VARVQGLLGS 

       310        320        330        340        350        360 
VDANQGDPLL GWDTDEFPTD LYSTTLAMYE ILQNGGLGSG GLNFDAKVRR GSFEQDDLLY 

       370        380        390        400        410        420 
AHVAGMDAFA RGLKVAHKLV EDRVFENVIN ERYSSFKEGI GLEIVEGKAN FHTLEQYAFK 

       430        440 
NPNIANKSGR QERLKSILNQ YILEV

References

[1]	"Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization." Rygus T., Scheler A., Allmansberger R., Hillen W. Arch. Microbiol. 155:535-542(1991) [PubMed: 1719948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 319.
[2]	"Regulation of expression, genetic organization and substrate specificity of xylose uptake in Bacillus megaterium." Schmiedel D., Kintrup M., Kuster E., Hillen W. Mol. Microbiol. 23:1053-1062(1997) [PubMed: 9076741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 319.

Cross-references

Sequence databases
	Z71474 Genomic DNA. Translation: CAA96094.1. X57598 Genomic DNA. Translation: CAA40821.1.
3D structure databases
HSSP	HSSP built from PDB template 1A0D based on UniProtKB P54273.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.3.1.5. 325.
Family and domain databases
HAMAP	MF_00455. [Tree]
InterPro	IPR013022. Xyl_isomerase-like_TIM-brl. IPR012307. Xyl_isomerase_TIM-brl. IPR013452. Xylose_isom_bac. IPR001998. Xylose_isomerase. IPR018115. Xylose_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PRINTS	PR00688. XYLOSISMRASE.
TIGRFAMs	TIGR02630. xylose_isom_A. 1 hit.
PROSITE	PS51415. XYLOSE_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLA_BACME

Accession

Primary (citable) accession number: O08325

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 1, 1997
Last modified:	September 22, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents