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Reviewed, UniProtKB/Swiss-Prot O08325 (XYLA_BACME)

Last modified September 22, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Xylose isomerase
    EC=5.3.1.5
Gene names
Name: xylA
OrganismBacillus megaterium
Taxonomic identifier1404 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesBacillaceaeBacillus

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

D-xylose = D-xylulose. HAMAP MF_00455

Cofactor

Binds 2 magnesium ions per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm. HAMAP MF_00455

Sequence similarities

Belongs to the xylose isomerase family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Pentose shunt
Xylose metabolism
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionIsomerase
Gene Ontology (GO)
   Biological processD-xylose metabolic process

Inferred from electronic annotation. Source: HAMAP

pentose-phosphate shunt

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionmagnesium ion binding

Inferred from electronic annotation. Source: HAMAP

xylose isomerase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445Xylose isomerase HAMAP MF_00455
PRO_0000195764

Sites

Active site1071 By similarity
Active site1101 By similarity
Metal binding2381Magnesium 1 By similarity
Metal binding2741Magnesium 1 By similarity
Metal binding2741Magnesium 2 By similarity
Metal binding2771Magnesium 2 By similarity
Metal binding3021Magnesium 1 By similarity
Metal binding3131Magnesium 2 By similarity
Metal binding3151Magnesium 2 By similarity
Metal binding3451Magnesium 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
O08325-1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 053A45A2B6B2C72E

FASTA44550,279
        10         20         30         40         50         60 
MVQTSTNKIN HFESANKVLY EGKDSKNPLA FKYYNPEEVV GGKTMKDQLR FSVAYWHQFT 

        70         80         90        100        110        120 
ADGTDPFGAA TMQRSWDRYD GMDLAKARVE AAFQLFETLN VPFFAFHDRD IAPEGSTLQE 

       130        140        150        160        170        180 
TNKNLDVIVT MIKEYMQTSN VKLLWNTANM FTNPRFVHGA ATSCNADVFR YAAAQVKKGL 

       190        200        210        220        230        240 
ETAKELGAEN YVFWGGREGY ETLLNTNLQL ELDNLARFMH MAVDYATEIG YTGQFLIEPK 

       250        260        270        280        290        300 
PKEPTTHQYD TDAATTISFL RQYGLDKYFK LNLEANHATL AGHTFEHELR VARVQGLLGS 

       310        320        330        340        350        360 
VDANQGDPLL GWDTDEFPTD LYSTTLAMYE ILQNGGLGSG GLNFDAKVRR GSFEQDDLLY 

       370        380        390        400        410        420 
AHVAGMDAFA RGLKVAHKLV EDRVFENVIN ERYSSFKEGI GLEIVEGKAN FHTLEQYAFK 

       430        440 
NPNIANKSGR QERLKSILNQ YILEV 

« Hide

References

[1]"Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization."
Rygus T., Scheler A., Allmansberger R., Hillen W.
Arch. Microbiol. 155:535-542(1991) [PubMed: 1719948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 319.
[2]"Regulation of expression, genetic organization and substrate specificity of xylose uptake in Bacillus megaterium."
Schmiedel D., Kintrup M., Kuster E., Hillen W.
Mol. Microbiol. 23:1053-1062(1997) [PubMed: 9076741] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 319.

Cross-references

Sequence databases

Z71474 Genomic DNA. Translation: CAA96094.1.
X57598 Genomic DNA. Translation: CAA40821.1.

3D structure databases

HSSPHSSP built from PDB template 1A0D based on UniProtKB P54273.
ModBaseSearch...

Enzyme and pathway databases

BRENDA5.3.1.5. 325.

Family and domain databases

HAMAPMF_00455.
[Tree]
InterProIPR013022. Xyl_isomerase-like_TIM-brl.
IPR012307. Xyl_isomerase_TIM-brl.
IPR013452. Xylose_isom_bac.
IPR001998. Xylose_isomerase.
IPR018115. Xylose_isomerase_AS.
[Graphical view]
Gene3DG3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
PfamPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
PRINTSPR00688. XYLOSISMRASE.
TIGRFAMsTIGR02630. xylose_isom_A. 1 hit.
PROSITEPS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXYLA_BACME
AccessionPrimary (citable) accession number: O08325
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 1, 1997
Last modified: September 22, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents