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O08323

- PYRF_MYCS2

UniProt

O08323 - PYRF_MYCS2

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Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrF

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Proton donorBy similarity

GO - Molecular functioni

  1. orotidine-5'-phosphate decarboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3048-MONOMER.
UniPathwayiUPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
Alternative name(s):
OMP decarboxylase
Short name:
OMPDCase
Short name:
OMPdecase
Gene namesi
Name:pyrF
Ordered Locus Names:MSMEG_3048, MSMEI_2974
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Orotidine 5'-phosphate decarboxylasePRO_0000134629Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_3048.

Structurei

3D structure databases

ProteinModelPortaliO08323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0284.
HOGENOMiHOG000245748.
KOiK01591.
OMAiGSTMGAY.
OrthoDBiEOG6DZF33.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01215. OMPdecase_type2.
InterProiIPR013785. Aldolase_TIM.
IPR018089. OMPdecase_AS.
IPR011995. OMPdecase_type-2.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR02127. pyrF_sub2. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08323-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGFGQRLDA AVSARGPLCP GIDPHPELLN AWGLTVDAEG LRAFCDICVA
60 70 80 90 100
AFAGFAIVKP QVAFFEAYGS AGFAVLEDTI AALRAEGVLV LADAKRGDIG
110 120 130 140 150
STMAAYAAAW AGDSPLAADA VTASPYLGFG SLRPLLDTAV ANGRGVFVLA
160 170 180 190 200
ATSNPEGVGL QRAVAGDVTV AQSIVDAVAQ ANREADPAAR DGDPVGPFGV
210 220 230 240 250
VVGATVADPP DLHMLGGPVL VPGVGAQGGR PEALGGLGNA RRLLPAVSRE
260 270
VLRAGPAVDD VRAAAERLRD QVAYLA
Length:276
Mass (Da):27,816
Last modified:July 1, 1997 - v1
Checksum:i41437843A3E2A896
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U91572 Genomic DNA. Translation: AAB50157.1.
CP000480 Genomic DNA. Translation: ABK73017.1.
CP001663 Genomic DNA. Translation: AFP39438.1.
RefSeqiYP_006567733.1. NC_018289.1.
YP_887364.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73017; ABK73017; MSMEG_3048.
AFP39438; AFP39438; MSMEI_2974.
GeneIDi4532520.
KEGGimsg:MSMEI_2974.
msm:MSMEG_3048.
PATRICi18078524. VBIMycSme59918_3010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U91572 Genomic DNA. Translation: AAB50157.1 .
CP000480 Genomic DNA. Translation: ABK73017.1 .
CP001663 Genomic DNA. Translation: AFP39438.1 .
RefSeqi YP_006567733.1. NC_018289.1.
YP_887364.1. NC_008596.1.

3D structure databases

ProteinModelPortali O08323.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_3048.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK73017 ; ABK73017 ; MSMEG_3048 .
AFP39438 ; AFP39438 ; MSMEI_2974 .
GeneIDi 4532520.
KEGGi msg:MSMEI_2974.
msm:MSMEG_3048.
PATRICi 18078524. VBIMycSme59918_3010.

Phylogenomic databases

eggNOGi COG0284.
HOGENOMi HOG000245748.
KOi K01591.
OMAi GSTMGAY.
OrthoDBi EOG6DZF33.

Enzyme and pathway databases

UniPathwayi UPA00070 ; UER00120 .
BioCyci MSME246196:GJ4Y-3048-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01215. OMPdecase_type2.
InterProi IPR013785. Aldolase_TIM.
IPR018089. OMPdecase_AS.
IPR011995. OMPdecase_type-2.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view ]
Pfami PF00215. OMPdecase. 1 hit.
[Graphical view ]
SMARTi SM00934. OMPdecase. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
TIGRFAMsi TIGR02127. pyrF_sub2. 1 hit.
PROSITEi PS00156. OMPDECASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Unmarked gene integration into the chromosome of Mycobacterium smegmatis via precise replacement of the pyrF gene."
    Knipfer N., Seth A., Shrader T.E.
    Plasmid 37:129-140(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiPYRF_MYCS2
AccessioniPrimary (citable) accession number: O08323
Secondary accession number(s): A0QWS6, I7FL41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: October 1, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3