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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11Carbamoyl phosphateBy similarity1
Binding sitei73Carbamoyl phosphateBy similarity1
Binding sitei84Carbamoyl phosphateBy similarity1
Binding sitei108Carbamoyl phosphateBy similarity1
Binding sitei167OrnithineBy similarity1
Binding sitei231OrnithineBy similarity1
Binding sitei299Carbamoyl phosphateBy similarity1
Binding sitei317Carbamoyl phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciLPLA220668:G137Z-442-MONOMER.
UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:lp_0532
OrganismiLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Taxonomic identifieri220668 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000000432 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001129371 – 340Ornithine carbamoyltransferaseAdd BLAST340

Interactioni

Protein-protein interaction databases

STRINGi220668.lp_0532.

Structurei

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Important for structural integrityBy similarity1
Sitei148Important for structural integrityBy similarity1

3D structure databases

ProteinModelPortaliO08322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 61Carbamoyl phosphate bindingBy similarity5
Regioni135 – 138Carbamoyl phosphate bindingBy similarity4
Regioni235 – 236Ornithine bindingBy similarity2
Regioni271 – 274Carbamoyl phosphate bindingBy similarity4

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiDFRIFAP.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08322-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNQFEGRSF LKEIDYTPTE LAYLIDFASH LKTLKAHHIP HPYLQGKNIA
60 70 80 90 100
LLFEKTSTRT RSAFTVAAND LGANPEFLCK DDIQFGTKES VVDTAKVLGS
110 120 130 140 150
MYDGIEFRGF KQSTVEDLAT YSGVPVWNGL TDEWHPTQIL ADFLTLKEHF
160 170 180 190 200
GHLKGLTLAY VGDGRNNMAN SLLVAGAMLG INIAIGSPVA LQPAPAVVAL
210 220 230 240 250
AQQYAQAAGS TVVITADPQQ AVTQADALYT DVWVSMGEQV DYGERIKQLL
260 270 280 290 300
PFQINTALLA ATGKSSTIVM HCLPALHDLK TQLGAKLGEQ YGMTAFEITD
310 320 330 340
DVFQSKQEVV FEEAGNRMPA IKAVMAATLG NLFIPTSIFN
Length:340
Mass (Da):36,974
Last modified:March 25, 2003 - v2
Checksum:i59BF46086D5620E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79C → G in CAA68243 (PubMed:9098069).Curated1
Sequence conflicti187S → A in CAA68243 (PubMed:9098069).Curated1
Sequence conflicti257A → T in CAA68243 (PubMed:9098069).Curated1
Sequence conflicti308E → A in CAA68243 (PubMed:9098069).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99978 Genomic DNA. Translation: CAA68243.1.
AL935263 Genomic DNA. Translation: CCC78022.1.
RefSeqiWP_011101046.1. NC_004567.2.
YP_004888536.1. NC_004567.2.

Genome annotation databases

EnsemblBacteriaiCCC78022; CCC78022; lp_0532.
GeneIDi1061878.
KEGGilpl:lp_0532.
PATRICi22247507. VBILacPla27411_0440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99978 Genomic DNA. Translation: CAA68243.1.
AL935263 Genomic DNA. Translation: CCC78022.1.
RefSeqiWP_011101046.1. NC_004567.2.
YP_004888536.1. NC_004567.2.

3D structure databases

ProteinModelPortaliO08322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220668.lp_0532.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCC78022; CCC78022; lp_0532.
GeneIDi1061878.
KEGGilpl:lp_0532.
PATRICi22247507. VBILacPla27411_0440.

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiDFRIFAP.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.
BioCyciLPLA220668:G137Z-442-MONOMER.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_LACPL
AccessioniPrimary (citable) accession number: O08322
Secondary accession number(s): F9UL07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: March 25, 2003
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.