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O08322 (OTC_LACPL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ornithine carbamoyltransferase

Short name=OTCase
EC=2.1.3.3
Gene names
Name:argF
Ordered Locus Names:lp_0532
OrganismLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) [Reference proteome] [HAMAP]
Taxonomic identifier220668 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline By similarity. HAMAP-Rule MF_01109

Catalytic activity

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP-Rule MF_01109

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP-Rule MF_01109

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01109.

Sequence similarities

Belongs to the ATCase/OTCase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionamino acid binding

Inferred from electronic annotation. Source: InterPro

ornithine carbamoyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Ornithine carbamoyltransferase HAMAP-Rule MF_01109
PRO_0000112937

Regions

Region57 – 615Carbamoyl phosphate binding By similarity
Region135 – 1384Carbamoyl phosphate binding By similarity
Region235 – 2362Ornithine binding By similarity
Region271 – 2744Carbamoyl phosphate binding By similarity

Sites

Binding site111Carbamoyl phosphate By similarity
Binding site731Carbamoyl phosphate By similarity
Binding site841Carbamoyl phosphate By similarity
Binding site1081Carbamoyl phosphate By similarity
Binding site1671Ornithine By similarity
Binding site2311Ornithine By similarity
Binding site2991Carbamoyl phosphate By similarity
Binding site3171Carbamoyl phosphate By similarity
Site321Important for structural integrity By similarity
Site1481Important for structural integrity By similarity

Experimental info

Sequence conflict791C → G in CAA68243. Ref.1
Sequence conflict1871S → A in CAA68243. Ref.1
Sequence conflict2571A → T in CAA68243. Ref.1
Sequence conflict3081E → A in CAA68243. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O08322 [UniParc].

Last modified March 25, 2003. Version 2.
Checksum: 59BF46086D5620E9

FASTA34036,974
        10         20         30         40         50         60 
MNNQFEGRSF LKEIDYTPTE LAYLIDFASH LKTLKAHHIP HPYLQGKNIA LLFEKTSTRT 

        70         80         90        100        110        120 
RSAFTVAAND LGANPEFLCK DDIQFGTKES VVDTAKVLGS MYDGIEFRGF KQSTVEDLAT 

       130        140        150        160        170        180 
YSGVPVWNGL TDEWHPTQIL ADFLTLKEHF GHLKGLTLAY VGDGRNNMAN SLLVAGAMLG 

       190        200        210        220        230        240 
INIAIGSPVA LQPAPAVVAL AQQYAQAAGS TVVITADPQQ AVTQADALYT DVWVSMGEQV 

       250        260        270        280        290        300 
DYGERIKQLL PFQINTALLA ATGKSSTIVM HCLPALHDLK TQLGAKLGEQ YGMTAFEITD 

       310        320        330        340 
DVFQSKQEVV FEEAGNRMPA IKAVMAATLG NLFIPTSIFN 

« Hide

References

« Hide 'large scale' references
[1]"Arginine biosynthesis and regulation in Lactobacillus plantarum: the carA gene and the argCJBDF cluster are divergently transcribed."
Bringel F., Frey L., Boivin S., Hubert J.-C.
J. Bacteriol. 179:2697-2706(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 8014 / CCM 1904 / DSM 20205 / NCDO 82 / NCIB 6376.
[2]"Complete genome sequence of Lactobacillus plantarum WCFS1."
Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J.
Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99978 Genomic DNA. Translation: CAA68243.1.
AL935263 Genomic DNA. Translation: CCC78022.1.
RefSeqYP_004888536.1. NC_004567.2.

3D structure databases

ProteinModelPortalO08322.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING220668.lp_0532.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCCC78022; CCC78022; lp_0532.
GeneID1061878.
KEGGlpl:lp_0532.
PATRIC22247507. VBILacPla27411_0440.

Phylogenomic databases

eggNOGCOG0078.
HOGENOMHOG000022686.
KOK00611.
OMAAFTVACT.
ProtClustDBPRK02102.

Enzyme and pathway databases

UniPathwayUPA00068; UER00112.

Family and domain databases

Gene3D3.40.50.1370. 2 hits.
HAMAPMF_01109. OTCase.
InterProIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMSSF53671. SSF53671. 1 hit.
TIGRFAMsTIGR00658. orni_carb_tr. 1 hit.
PROSITEPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOTC_LACPL
AccessionPrimary (citable) accession number: O08322
Secondary accession number(s): F9UL07
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: March 25, 2003
Last modified: February 19, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways