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O08309 (KPYK_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pyk
Synonyms:pykA
Ordered Locus Names:CA_C0518
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 473473Pyruvate kinase
PRO_0000112064

Sites

Metal binding341Potassium By similarity
Metal binding361Potassium By similarity
Metal binding661Potassium By similarity
Metal binding671Potassium; via carbonyl oxygen By similarity
Metal binding2211Magnesium By similarity
Metal binding2451Magnesium By similarity
Binding site321Substrate By similarity
Binding site2441Substrate; via amide nitrogen By similarity
Binding site2451Substrate; via amide nitrogen By similarity
Binding site2771Substrate By similarity
Site2191Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
O08309 [UniParc].

Last modified August 29, 2001. Version 2.
Checksum: 1A240576A91EEA43

FASTA47350,592
        10         20         30         40         50         60 
MQKTKMIFTV GPASETEEIV TAFIKAGMNA SRHNFSHGDH AEHGGRIALV KKVRAKLNKP 

        70         80         90        100        110        120 
VAICLDTKGP EIRTGDFNPS KLELQKGSKF TIVCGEEIVG DATKCSISYK DLYKDVKPGN 

       130        140        150        160        170        180 
TILIDDGLVG LTVEAIEGTN VICTVANTGL VGSHKGVNVP NVSIQLPAMT EKDKSDLIFG 

       190        200        210        220        230        240 
CKEEIDMVSA SFIRKPEDVL AIRKVLNENG GENIQIFSKI ENQEGVDNID AIIEVSDGIM 

       250        260        270        280        290        300 
VARGDMGVEI PIQRVPLIQK MIIKKCNAVG KPVITATQML DSMMRNPRPT RAEASDIANA 

       310        320        330        340        350        360 
IFDGTDAIML SGESANGSYP IEAVTTMAKI AQEAENEINY DKFLAERKGN EKKNTSDVIS 

       370        380        390        400        410        420 
LGTCTAAADL EASAIITATQ TGSTARTVSK YRPKAPVIAV TPSEKVARKL AMSWGVHPII 

       430        440        450        460        470 
SDKFGSTDEL ISTSVDKALE AGYVQKGDLV VVAAGVPTNV SGTTNMLKVQ VVE 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]"Cloning, sequence, and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli."
Belouski E., Watson D.E., Bennett G.N.
Curr. Microbiol. 37:17-22(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001437 Genomic DNA. Translation: AAK78498.1.
U52366 Genomic DNA. Translation: AAB50190.1.
PIRG96963.
RefSeqNP_347158.1. NC_003030.1.

3D structure databases

ProteinModelPortalO08309.
SMRO08309. Positions 2-472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C0518.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK78498; AAK78498; CA_C0518.
GeneID1116701.
KEGGcac:CA_C0518.
PATRIC32035391. VBICloAce74127_0717.

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021559.
KOK00873.
OMANNKGVNF.
OrthoDBEOG6GBMB0.
ProtClustDBCLSK801320.

Enzyme and pathway databases

BioCycCACE272562:GJIH-559-MONOMER.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKPYK_CLOAB
AccessionPrimary (citable) accession number: O08309
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 29, 2001
Last modified: February 19, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways