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O08309

- KPYK_CLOAB

UniProt

O08309 - KPYK_CLOAB

Protein

Pyruvate kinase

Gene

pyk

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (29 Aug 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321SubstrateBy similarity
    Metal bindingi34 – 341PotassiumBy similarity
    Metal bindingi36 – 361PotassiumBy similarity
    Metal bindingi66 – 661PotassiumBy similarity
    Metal bindingi67 – 671Potassium; via carbonyl oxygenBy similarity
    Sitei219 – 2191Transition state stabilizerBy similarity
    Metal bindingi221 – 2211MagnesiumBy similarity
    Binding sitei244 – 2441Substrate; via amide nitrogenBy similarity
    Metal bindingi245 – 2451MagnesiumBy similarity
    Binding sitei245 – 2451Substrate; via amide nitrogenBy similarity
    Binding sitei277 – 2771SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciCACE272562:GJIH-559-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    Synonyms:pykA
    Ordered Locus Names:CA_C0518
    OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
    Taxonomic identifieri272562 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000814: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473Pyruvate kinasePRO_0000112064Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi272562.CA_C0518.

    Structurei

    3D structure databases

    ProteinModelPortaliO08309.
    SMRiO08309. Positions 2-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    KOiK00873.
    OMAiGTHEEHK.
    OrthoDBiEOG6GBMB0.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O08309-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQKTKMIFTV GPASETEEIV TAFIKAGMNA SRHNFSHGDH AEHGGRIALV    50
    KKVRAKLNKP VAICLDTKGP EIRTGDFNPS KLELQKGSKF TIVCGEEIVG 100
    DATKCSISYK DLYKDVKPGN TILIDDGLVG LTVEAIEGTN VICTVANTGL 150
    VGSHKGVNVP NVSIQLPAMT EKDKSDLIFG CKEEIDMVSA SFIRKPEDVL 200
    AIRKVLNENG GENIQIFSKI ENQEGVDNID AIIEVSDGIM VARGDMGVEI 250
    PIQRVPLIQK MIIKKCNAVG KPVITATQML DSMMRNPRPT RAEASDIANA 300
    IFDGTDAIML SGESANGSYP IEAVTTMAKI AQEAENEINY DKFLAERKGN 350
    EKKNTSDVIS LGTCTAAADL EASAIITATQ TGSTARTVSK YRPKAPVIAV 400
    TPSEKVARKL AMSWGVHPII SDKFGSTDEL ISTSVDKALE AGYVQKGDLV 450
    VVAAGVPTNV SGTTNMLKVQ VVE 473
    Length:473
    Mass (Da):50,592
    Last modified:August 29, 2001 - v2
    Checksum:i1A240576A91EEA43
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001437 Genomic DNA. Translation: AAK78498.1.
    U52366 Genomic DNA. Translation: AAB50190.1.
    PIRiG96963.
    RefSeqiNP_347158.1. NC_003030.1.

    Genome annotation databases

    EnsemblBacteriaiAAK78498; AAK78498; CA_C0518.
    GeneIDi1116701.
    KEGGicac:CA_C0518.
    PATRICi32035391. VBICloAce74127_0717.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001437 Genomic DNA. Translation: AAK78498.1 .
    U52366 Genomic DNA. Translation: AAB50190.1 .
    PIRi G96963.
    RefSeqi NP_347158.1. NC_003030.1.

    3D structure databases

    ProteinModelPortali O08309.
    SMRi O08309. Positions 2-472.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272562.CA_C0518.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK78498 ; AAK78498 ; CA_C0518 .
    GeneIDi 1116701.
    KEGGi cac:CA_C0518.
    PATRICi 32035391. VBICloAce74127_0717.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    KOi K00873.
    OMAi GTHEEHK.
    OrthoDBi EOG6GBMB0.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci CACE272562:GJIH-559-MONOMER.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    2. "Cloning, sequence, and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli."
      Belouski E., Watson D.E., Bennett G.N.
      Curr. Microbiol. 37:17-22(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

    Entry informationi

    Entry nameiKPYK_CLOAB
    AccessioniPrimary (citable) accession number: O08309
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: August 29, 2001
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3