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O08309

- KPYK_CLOAB

UniProt

O08309 - KPYK_CLOAB

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Protein
Pyruvate kinase
Gene
pyk, pykA, CA_C0518
Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium By similarity.
Potassium By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321Substrate By similarity
Metal bindingi34 – 341Potassium By similarity
Metal bindingi36 – 361Potassium By similarity
Metal bindingi66 – 661Potassium By similarity
Metal bindingi67 – 671Potassium; via carbonyl oxygen By similarity
Sitei219 – 2191Transition state stabilizer By similarity
Metal bindingi221 – 2211Magnesium By similarity
Binding sitei244 – 2441Substrate; via amide nitrogen By similarity
Metal bindingi245 – 2451Magnesium By similarity
Binding sitei245 – 2451Substrate; via amide nitrogen By similarity
Binding sitei277 – 2771Substrate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciCACE272562:GJIH-559-MONOMER.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Synonyms:pykA
Ordered Locus Names:CA_C0518
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Pyruvate kinase
PRO_0000112064Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi272562.CA_C0518.

Structurei

3D structure databases

ProteinModelPortaliO08309.
SMRiO08309. Positions 2-472.

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
KOiK00873.
OMAiGTHEEHK.
OrthoDBiEOG6GBMB0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

Sequencei

Sequence statusi: Complete.

O08309-1 [UniParc]FASTAAdd to Basket

« Hide

MQKTKMIFTV GPASETEEIV TAFIKAGMNA SRHNFSHGDH AEHGGRIALV    50
KKVRAKLNKP VAICLDTKGP EIRTGDFNPS KLELQKGSKF TIVCGEEIVG 100
DATKCSISYK DLYKDVKPGN TILIDDGLVG LTVEAIEGTN VICTVANTGL 150
VGSHKGVNVP NVSIQLPAMT EKDKSDLIFG CKEEIDMVSA SFIRKPEDVL 200
AIRKVLNENG GENIQIFSKI ENQEGVDNID AIIEVSDGIM VARGDMGVEI 250
PIQRVPLIQK MIIKKCNAVG KPVITATQML DSMMRNPRPT RAEASDIANA 300
IFDGTDAIML SGESANGSYP IEAVTTMAKI AQEAENEINY DKFLAERKGN 350
EKKNTSDVIS LGTCTAAADL EASAIITATQ TGSTARTVSK YRPKAPVIAV 400
TPSEKVARKL AMSWGVHPII SDKFGSTDEL ISTSVDKALE AGYVQKGDLV 450
VVAAGVPTNV SGTTNMLKVQ VVE 473
Length:473
Mass (Da):50,592
Last modified:August 29, 2001 - v2
Checksum:i1A240576A91EEA43
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001437 Genomic DNA. Translation: AAK78498.1.
U52366 Genomic DNA. Translation: AAB50190.1.
PIRiG96963.
RefSeqiNP_347158.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK78498; AAK78498; CA_C0518.
GeneIDi1116701.
KEGGicac:CA_C0518.
PATRICi32035391. VBICloAce74127_0717.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001437 Genomic DNA. Translation: AAK78498.1 .
U52366 Genomic DNA. Translation: AAB50190.1 .
PIRi G96963.
RefSeqi NP_347158.1. NC_003030.1.

3D structure databases

ProteinModelPortali O08309.
SMRi O08309. Positions 2-472.
ModBasei Search...

Protein-protein interaction databases

STRINGi 272562.CA_C0518.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK78498 ; AAK78498 ; CA_C0518 .
GeneIDi 1116701.
KEGGi cac:CA_C0518.
PATRICi 32035391. VBICloAce74127_0717.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021559.
KOi K00873.
OMAi GTHEEHK.
OrthoDBi EOG6GBMB0.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
BioCyci CACE272562:GJIH-559-MONOMER.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  2. "Cloning, sequence, and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli."
    Belouski E., Watson D.E., Bennett G.N.
    Curr. Microbiol. 37:17-22(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiKPYK_CLOAB
AccessioniPrimary (citable) accession number: O08309
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 29, 2001
Last modified: June 11, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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