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O08309

- KPYK_CLOAB

UniProt

O08309 - KPYK_CLOAB

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Protein

Pyruvate kinase

Gene

pyk

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.By similarity
Potassium.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321SubstrateBy similarity
Metal bindingi34 – 341PotassiumBy similarity
Metal bindingi36 – 361PotassiumBy similarity
Metal bindingi66 – 661PotassiumBy similarity
Metal bindingi67 – 671Potassium; via carbonyl oxygenBy similarity
Sitei219 – 2191Transition state stabilizerBy similarity
Metal bindingi221 – 2211MagnesiumBy similarity
Binding sitei244 – 2441Substrate; via amide nitrogenBy similarity
Metal bindingi245 – 2451MagnesiumBy similarity
Binding sitei245 – 2451Substrate; via amide nitrogenBy similarity
Binding sitei277 – 2771SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciCACE272562:GJIH-559-MONOMER.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Synonyms:pykA
Ordered Locus Names:CA_C0518
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Pyruvate kinasePRO_0000112064Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272562.CA_C0518.

Structurei

3D structure databases

ProteinModelPortaliO08309.
SMRiO08309. Positions 2-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
KOiK00873.
OMAiGTHEEHK.
OrthoDBiEOG6GBMB0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

Sequencei

Sequence statusi: Complete.

O08309-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQKTKMIFTV GPASETEEIV TAFIKAGMNA SRHNFSHGDH AEHGGRIALV
60 70 80 90 100
KKVRAKLNKP VAICLDTKGP EIRTGDFNPS KLELQKGSKF TIVCGEEIVG
110 120 130 140 150
DATKCSISYK DLYKDVKPGN TILIDDGLVG LTVEAIEGTN VICTVANTGL
160 170 180 190 200
VGSHKGVNVP NVSIQLPAMT EKDKSDLIFG CKEEIDMVSA SFIRKPEDVL
210 220 230 240 250
AIRKVLNENG GENIQIFSKI ENQEGVDNID AIIEVSDGIM VARGDMGVEI
260 270 280 290 300
PIQRVPLIQK MIIKKCNAVG KPVITATQML DSMMRNPRPT RAEASDIANA
310 320 330 340 350
IFDGTDAIML SGESANGSYP IEAVTTMAKI AQEAENEINY DKFLAERKGN
360 370 380 390 400
EKKNTSDVIS LGTCTAAADL EASAIITATQ TGSTARTVSK YRPKAPVIAV
410 420 430 440 450
TPSEKVARKL AMSWGVHPII SDKFGSTDEL ISTSVDKALE AGYVQKGDLV
460 470
VVAAGVPTNV SGTTNMLKVQ VVE
Length:473
Mass (Da):50,592
Last modified:August 29, 2001 - v2
Checksum:i1A240576A91EEA43
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001437 Genomic DNA. Translation: AAK78498.1.
U52366 Genomic DNA. Translation: AAB50190.1.
PIRiG96963.
RefSeqiNP_347158.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK78498; AAK78498; CA_C0518.
GeneIDi1116701.
KEGGicac:CA_C0518.
PATRICi32035391. VBICloAce74127_0717.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001437 Genomic DNA. Translation: AAK78498.1 .
U52366 Genomic DNA. Translation: AAB50190.1 .
PIRi G96963.
RefSeqi NP_347158.1. NC_003030.1.

3D structure databases

ProteinModelPortali O08309.
SMRi O08309. Positions 2-472.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272562.CA_C0518.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK78498 ; AAK78498 ; CA_C0518 .
GeneIDi 1116701.
KEGGi cac:CA_C0518.
PATRICi 32035391. VBICloAce74127_0717.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021559.
KOi K00873.
OMAi GTHEEHK.
OrthoDBi EOG6GBMB0.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
BioCyci CACE272562:GJIH-559-MONOMER.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  2. "Cloning, sequence, and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli."
    Belouski E., Watson D.E., Bennett G.N.
    Curr. Microbiol. 37:17-22(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiKPYK_CLOAB
AccessioniPrimary (citable) accession number: O08309
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 29, 2001
Last modified: October 1, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3