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O08308 (K6PF_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphofructokinase

Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Synonyms:pfk
Ordered Locus Names:CA_C0517
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3193196-phosphofructokinase HAMAP-Rule MF_00339
PRO_0000111943

Regions

Nucleotide binding21 – 255ATP By similarity
Nucleotide binding154 – 1585ATP By similarity
Nucleotide binding171 – 18717ATP By similarity

Sites

Active site1271Proton acceptor By similarity
Metal binding1851Magnesium; via carbonyl oxygen By similarity
Metal binding1871Magnesium By similarity
Binding site1621Substrate By similarity
Binding site2431Substrate By similarity
Binding site2491Substrate By similarity
Binding site2521Substrate By similarity

Experimental info

Sequence conflict391R → G in AAB50189. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O08308 [UniParc].

Last modified August 29, 2001. Version 2.
Checksum: 7E138D7367EB3835

FASTA31934,194
        10         20         30         40         50         60 
MKTIAVLTSG GDAPGMNAAI RAVVRTAIEK GINVKGIQRG YSGLINGEIF DMNRHSVSDI 

        70         80         90        100        110        120 
IQRGGTILRT ARCPEFLKEE VRQKAANVLR VFGIDGLVVI GGNGSFMGAQ KLSKLGVKTV 

       130        140        150        160        170        180 
GLPGTIDNDL PYTDYTIGFD TTLNTVLDAI NKLRDTSTSH ERVSIIEVMG RDCGDIALFS 

       190        200        210        220        230        240 
GIAGGAESVI IPEIGYDFNE LCKNILEGKL RGKMHNLIIL AEGVGGAAEL AKKVEEVTGI 

       250        260        270        280        290        300 
ETRSTILGHI QRGGSPSAFD RMLASRMGVK AVEVLMEGKT SRVIGIKEGK IMDQDIDEAL 

       310 
AVPRSFNKEL YDIANMLSK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequence, and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli."
Belouski E., Watson D.E., Bennett G.N.
Curr. Microbiol. 37:17-22(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52366 Genomic DNA. Translation: AAB50189.1.
AE001437 Genomic DNA. Translation: AAK78497.1.
PIRF96963.
RefSeqNP_347157.1. NC_003030.1.

3D structure databases

ProteinModelPortalO08308.
SMRO08308. Positions 1-318.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C0517.

Proteomic databases

PRIDEO08308.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK78497; AAK78497; CA_C0517.
GeneID1116700.
KEGGcac:CA_C0517.
PATRIC32035389. VBICloAce74127_0716.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248870.
KOK00850.
OMAKMVHHDI.
OrthoDBEOG644ZRM.
ProtClustDBPRK03202.

Enzyme and pathway databases

BioCycCACE272562:GJIH-558-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK6PF_CLOAB
AccessionPrimary (citable) accession number: O08308
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 29, 2001
Last modified: April 16, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways