O08308 (K6PF_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Synonyms:	pfk
Ordered Locus Names:	CA_C0517

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity HAMAP MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	6-phosphofructokinase HAMAP MF_00339		PRO_0000111943
Regions
	Nucleotide binding	21 – 25	5	ATP By similarity
	Nucleotide binding	154 – 158	5	ATP By similarity
	Nucleotide binding	171 – 187	17	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Metal binding	185	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	187	1	Magnesium By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
Experimental info
	Sequence conflict	39	1	R → G in AAB50189. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O08308 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 7E138D7367EB3835	FASTA	319	34,194
	10 20 30 40 50 60 MKTIAVLTSG GDAPGMNAAI RAVVRTAIEK GINVKGIQRG YSGLINGEIF DMNRHSVSDI 70 80 90 100 110 120 IQRGGTILRT ARCPEFLKEE VRQKAANVLR VFGIDGLVVI GGNGSFMGAQ KLSKLGVKTV 130 140 150 160 170 180 GLPGTIDNDL PYTDYTIGFD TTLNTVLDAI NKLRDTSTSH ERVSIIEVMG RDCGDIALFS 190 200 210 220 230 240 GIAGGAESVI IPEIGYDFNE LCKNILEGKL RGKMHNLIIL AEGVGGAAEL AKKVEEVTGI 250 260 270 280 290 300 ETRSTILGHI QRGGSPSAFD RMLASRMGVK AVEVLMEGKT SRVIGIKEGK IMDQDIDEAL 310 AVPRSFNKEL YDIANMLSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequence, and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli." Belouski E., Watson D.E., Bennett G.N. Curr. Microbiol. 37:17-22(1998) [PubMed: 9625784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52366 Genomic DNA. Translation: AAB50189.1. AE001437 Genomic DNA. Translation: AAK78497.1.
PIR	F96963.
RefSeq	NP_347157.1. NC_003030.1.
3D structure databases
ProteinModelPortal	O08308.
SMR	O08308. Positions 1-318.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1116700.
GenomeReviews	Gene locus CA_C0517 in contig AE001437_GR.
KEGG	cac:CA_C0517.
NMPDR	fig|272562.1.peg.686.
PATRIC	32035389. VBICloAce74127_0716.
Phylogenomic databases
HOGENOM	HBG405044.
OMA	MVHHDII.
PhylomeDB	O08308.
ProtClustDB	PRK03202.
Enzyme and pathway databases
BioCyc	CACE272562:CAC0517-MONOMER.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
KO	K00850.
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_CLOAB

Accession

Primary (citable) accession number: O08308

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	August 29, 2001
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program