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O07948 (NUOF_RHOCA) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit F

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit F
NDH-1 subunit F
Gene names
Name:nuoF
OrganismRhodobacter capsulatus (Rhodopseudomonas capsulata)
Taxonomic identifier1061 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length431 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activity

NADH + quinone = NAD+ + quinol.

Cofactor

Binds 1 FMN Potential.

Binds 1 4Fe-4S cluster Potential.

Sequence similarities

Belongs to the complex I 51 kDa subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 431431NADH-quinone oxidoreductase subunit F
PRO_0000118578

Regions

Nucleotide binding54 – 6310NAD By similarity
Nucleotide binding167 – 21448FMN By similarity

Sites

Metal binding3461Iron-sulfur (4Fe-4S) Potential
Metal binding3491Iron-sulfur (4Fe-4S) Potential
Metal binding3521Iron-sulfur (4Fe-4S) Potential
Metal binding3921Iron-sulfur (4Fe-4S) Potential

Sequences

Sequence LengthMass (Da)Tools
O07948 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 08AFA168C18BF8B4

FASTA43147,133
        10         20         30         40         50         60 
MLNDHDRIFT NIYGMHDRSL KGAMARGHWD GTAAILGNGR DWIIEQVKAS GLRGRGGAGF 

        70         80         90        100        110        120 
PTGLKWSFMP KQSDGRPSFL VINADESEPG TCKDREIMRH DPHTLIEGAL IAGFAMGARA 

       130        140        150        160        170        180 
AYIYIRGEYV REKEALQAAI DECYEAGLIG KNACRSDYDF DVYLHHGAGA YICGEETALL 

       190        200        210        220        230        240 
ESLEGKKGMP RMKPPFPAGS GLYGCPTTVN NVESIAVVPT ILRRGGAWFA GIGRPNNTGV 

       250        260        270        280        290        300 
KLFAMSGHVN TPCVVEEAMS ISMKELIEKH GGGVRGGWKN LKAVIPGGAS CPVIPAHLCE 

       310        320        330        340        350        360 
DAIMDYDGMR EKQSSFGTAC LIVMDQQTDI IKAIARLSKF FKHESCGQCT PCREGTSWMW 

       370        380        390        400        410        420 
RVMERLVTGE AEVEEIDMLF SVTKQVEGHT ICALGDAAAW PIQGLIRHYR EEIEDRIKAR 

       430 
KTGRMGAMAA E 

« Hide

References

[1]"Immuno-purification of a dimeric subcomplex of the respiratory NADH-CoQ reductase of Rhodobacter capsulatus equivalent to the FP fraction of the mitochondrial complex I."
Duborjal H., Dupuis A., Chapel A., Kieffer S., Lunardi J., Issartel J.P.
FEBS Lett. 405:345-350(1997) [PubMed: 9108316] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 33303 / B10.
[2]"The NADH-binding fragment of the NADH:ubiquinone oxidoreductase from Rhodobacter capsulatus: proteins and gene structure."
Herter S.M., Schiltz E., Drews G.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y10142 Genomic DNA. Translation: CAA71232.1.
Y09884 Genomic DNA. Translation: CAA71013.1.
AF029365 Genomic DNA. Translation: AAC24991.1.

3D structure databases

ProteinModelPortalO07948.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. NADH_UbQ_OxRdtase_51kDa_su.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsTIGR01959. NuoF_fam. 1 hit.
PROSITEPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNUOF_RHOCA
AccessionPrimary (citable) accession number: O07948
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: September 21, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families