O07899 (VIBE_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase
EC=6.3.2.-

Alternative name(s):
Dihydroxybenzoic acid-activating enzyme

Gene names

Name:	vibE
Ordered Locus Names:	VC_0772

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activation of the carboxylate group of 2,3-dihydroxy-benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate, preparing that molecule for the final stages of vibriobactin synthesis By similarity.
Pathway	Siderophore biosynthesis; vibriobactin biosynthesis.
Subcellular location	Cell inner membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	siderophore biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	(2,3-dihydroxybenzoyl)adenylate synthase activity Inferred from electronic annotation. Source: InterPro ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase		PRO_0000193080
Regions
	Transmembrane	240 – 259	20	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	O07899 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 1532583565B8C647	FASTA	543	60,065
	10 20 30 40 50 60 MTTDFTPWPE ALAAQYRQLG YWQDKTLLDY LQQSAERTPN ALALVGDNQQ WRYQAMLERI 70 80 90 100 110 120 EQLAAGFTEL GLGCGDNVVL QLGNVAEFYL CFFALLRQGI RPILALPAHR LAEIRYFCQH 130 140 150 160 170 180 SQAKAYLIDG AQRPFDYQAL AQELLACCPT LQTVIVRGQT RVTDPKFIEL ASCYSASSCQ 190 200 210 220 230 240 ANADPNQIAF FQLSGGTTGT PKLIPRTHND YAYSVTASVE ICRFDQHTRY LCVLPAAHNF 250 260 270 280 290 300 PLSSPGALGV FWAGGCVVLS QDASPQHAFK LIEQHKITVT ALVPPLALLW MDHAEKSTYD 310 320 330 340 350 360 LSSLHFVQVG GAKFSEAAAR RLPKALGCQL QQVFGMAEGL VNYTRLDDSA ELIATTQGRP 370 380 390 400 410 420 ISAHDQLLVV DEQGQPVASG EEGYLLTQGP YTIRGYYRAD QHNQRAFNAQ GFYITGDKVK 430 440 450 460 470 480 LSSEGYVIVT GRAKDQINRG GEKIAAEEVE NQLLHHPAVH DAALIAISDE YLGERSCAVI 490 500 510 520 530 540 VLKPEQSVNT IQLKRFLHQA GLADYKIPDQ IQFIDQLPKT SVGKIDKNAL RRRFDTLGLA LMS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a Vibrio cholerae vibriobactin gene cluster: identification of genes required for early steps in siderophore biosynthesis." Wyckoff E.E., Stoebner J.A., Reed K.E., Payne S.M. J. Bacteriol. 179:7055-7062(1997) [PubMed: 9371453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: El Tor Lou15.
[2]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52150 Genomic DNA. Translation: AAC45927.1. AE003852 Genomic DNA. Translation: AAF93937.1.
PIR	H82282.
RefSeq	NP_230421.1. NC_002505.1.
3D structure databases
ProteinModelPortal	O07899.
SMR	O07899. Positions 1-536.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2615315.
GenomeReviews	Gene locus VC_0772 in contig AE003852_GR.
KEGG	vch:VC0772.
PATRIC	20080655. VBIVibCho83274_0736.
TIGR	VC_0772.
Phylogenomic databases
HOGENOM	HBG547964.
OMA	QVGGAKC.
PhylomeDB	O07899.
ProtClustDB	CLSK793764.
Enzyme and pathway databases
BioCyc	MetaCyc:VC0772-MONOMER. VCHO:VC0772-MONOMER.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR011963. DHB_AMP_lig. [Graphical view]
KO	K04776.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02275. DHB_AMP_lig. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VIBE_VIBCH

Accession

Primary (citable) accession number: O07899
Secondary accession number(s): Q9JQ10

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program