Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot O07835 (IORA_PYRKO)

Last modified June 16, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Indolepyruvate oxidoreductase subunit iorA
      Short name=IOR
    EC=1.2.7.8
Alternative name(s):
    Indolepyruvate ferredoxin oxidoreductase subunit alpha
Gene names
Name: iorA
Ordered Locus Names: TK0136
OrganismPyrococcus kodakaraensis (Thermococcus kodakaraensis) [Complete proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Hide | Top

Protein attributes

Sequence length647 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.

Catalytic activity

(Indol-3-yl)pyruvate + CoA + 2 oxidized ferredoxin = S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+.

Cofactor

Binds 1 4Fe-4S cluster.

Subunit structure

Heterodimer of the iorA and iorB subunits.

Sequence similarities

Contains 2 4Fe-4S ferredoxin-type domains.

Biophysicochemical properties

Temperature dependence:

Optimum temperature is 70 degrees Celsius.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 647647Indolepyruvate oxidoreductase subunit iorA
PRO_0000099930

Regions

Domain585 – 614304Fe-4S ferredoxin-type 1
Domain616 – 645304Fe-4S ferredoxin-type 2

Sites

Metal binding6251Iron-sulfur (4Fe-4S) Potential
Metal binding6281Iron-sulfur (4Fe-4S) Potential
Metal binding6311Iron-sulfur (4Fe-4S) Potential
Metal binding6351Iron-sulfur (4Fe-4S) Potential

Experimental info

Sequence conflict261A → E in BAA20528. Ref.1
Sequence conflict50 – 545DTMAA → IRWQT in BAA20528. Ref.1
Sequence conflict3111P → R in BAA20528. Ref.1
Sequence conflict602 – 6032NA → KT in BAA20528. Ref.1

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
O07835-1 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: AE24D283C96CDBF7

FASTA64770,900
        10         20         30         40         50         60 
MAKVTDIVLW DKPGERVLLL GNHAIARGAL EANIAVFAAY PGTPSSELTD TMAAVAKKAG 

        70         80         90        100        110        120 
VYMEYSTNEK VAFETALAAA WSGLRAMTAM KHVGLNVAAD SFLSSVGMGV EGGFVIMVAD 

       130        140        150        160        170        180 
DPSMWSSQNE QDTRVYAKFA NVPVLEPSSP HEAKEMTKYA FELSEKFKHF VILRTTTRSS 

       190        200        210        220        230        240 
HARGDVVLGE LPEEIKTGKR KFGKFKKDPT RFVDVPAHAR KFHPLILEKI EKIREELNNC 

       250        260        270        280        290        300 
PFNWIEGKED AKVGIIAPGL SYAYVKEALA WLGVEDVKIL KLGTPFPVPY GLLGKFFDGL 

       310        320        330        340        350        360 
EKVLIVEELE PVVEEQVKTW AYDKGLRIPI HGKDLVPRVY EMTTRRAVEA IAKFLGLETP 

       370        380        390        400        410        420 
INFAEIDEKY EKVSQIVPPR PPSLCPACPH RNSFFAIRKA AGPKAIYPSD IGCYTLGVLP 

       430        440        450        460        470        480 
PLRTVDTTVA MGASIGIGHG LSIAMNGSLA EEEHKEGKEK QIIVATIGDS TFYHTGLPAL 

       490        500        510        520        530        540 
ANAIYNRSNV LIVVLDNLVT AMTGDQPNPG TGQTPHGMGK RIPIEDVAKA MGADFVAVVD 

       550        560        570        580        590        600 
PYDIKATYET IKKALEVEGV SVVVSRQVCA LYKIGQMRRR GMKWPIYHVV EDKCTGCKIC 

       610        620        630        640 
INAYGCPAIY WDPETKKAKV DPTMCWGCGG CAQVCPFDAF EPMKEGE

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Indolepyruvate ferredoxin oxidoreductase from Pyrococcus sp. KOD1 possesses a mosaic structure showing features of various oxidoreductases."
Siddiqui M.A., Fujiwara S., Imanaka T.
Mol. Gen. Genet. 254:433-439(1997) [PubMed: 9180697] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: KOD1.
[2]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KOD1.

Hide | Top

Cross-references

Sequence databases

D86221 Genomic DNA. Translation: BAA20528.1.
AP006878 Genomic DNA. Translation: BAD84325.1.
RefSeqYP_182549.1.

3D structure databases

HSSPHSSP built from PDB template 1JNR based on UniProtKB O28604.
ModBaseSearch...

Genome annotation databases

GeneID3235682.
GenomeReviewsGene locus TK0136 in contig AP006878_GR.
KEGGtko:TK0136.
NMPDRfig|69014.3.peg.304.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMO07835.
OMAO07835. VADDPSM.

Enzyme and pathway databases

BioCycTKOD69014:TK0136-MON.
BRENDA1.2.7.8. 192130.

Family and domain databases

InterProIPR017896. 4Fe4S_Fe-S-bd.
IPR001450. 4Fe4S_Fe_S_bd_subgr.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017721. Indolepyruvate_Fd_OxRdtase_asu.
IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR011766. TPP_enzyme_bd_C.
[Graphical view]
PfamPF00037. Fer4. 1 hit.
PF01855. POR_N. 1 hit.
PF02775. TPP_enzyme_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR03336. IOR_alpha. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameIORA_PYRKO
AccessionPrimary (citable) accession number: O07835
Secondary accession number(s): Q5JFI0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 29, 2005
Last modified: June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents