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Protein

Indolepyruvate oxidoreductase subunit IorA

Gene

iorA

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.

Catalytic activityi

(Indol-3-yl)pyruvate + CoA + 2 oxidized ferredoxin = S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+.

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters. In this family the first cluster has a non-standard and varying [4Fe-4S] binding motif CX(2)CX(2)CX(4-5)CP.1 Publication

Temperature dependencei

Optimum temperature is 70 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi594 – 5941Iron-sulfur 1 (4Fe-4S)1 Publication
Metal bindingi597 – 5971Iron-sulfur 1 (4Fe-4S)1 Publication
Metal bindingi600 – 6001Iron-sulfur 1 (4Fe-4S)1 Publication
Metal bindingi606 – 6061Iron-sulfur 2 (4Fe-4S)1 Publication
Metal bindingi625 – 6251Iron-sulfur 2 (4Fe-4S)1 Publication
Metal bindingi628 – 6281Iron-sulfur 2 (4Fe-4S)1 Publication
Metal bindingi631 – 6311Iron-sulfur 2 (4Fe-4S)1 Publication
Metal bindingi635 – 6351Iron-sulfur 1 (4Fe-4S)1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciTKOD69014:GH72-138-MONOMER.
BRENDAi1.2.7.8. 5246.

Names & Taxonomyi

Protein namesi
Recommended name:
Indolepyruvate oxidoreductase subunit IorA (EC:1.2.7.8)
Short name:
IOR
Alternative name(s):
Indolepyruvate ferredoxin oxidoreductase subunit alpha
Gene namesi
Name:iorA
Ordered Locus Names:TK0136
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 647647Indolepyruvate oxidoreductase subunit IorAPRO_0000099930Add
BLAST

Interactioni

Subunit structurei

Heterodimer of the IorA and IorB subunits.

Protein-protein interaction databases

STRINGi69014.TK0136.

Structurei

3D structure databases

ProteinModelPortaliO07835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini585 – 614304Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini616 – 645304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiarCOG01609. Archaea.
COG4231. LUCA.
HOGENOMiHOG000224871.
InParanoidiO07835.
KOiK00179.
OMAiMMPAMAR.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017721. Indolepyruvate_Fd_OxRdtase_asu.
IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR029061. THDP-binding.
IPR011766. TPP_enzyme-bd_C.
IPR009014. Transketo_C/PFOR_II.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
PF01855. POR_N. 1 hit.
PF02775. TPP_enzyme_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006439. Indolepyruvate_ferr_oxidored. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
TIGRFAMsiTIGR03336. IOR_alpha. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O07835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVTDIVLW DKPGERVLLL GNHAIARGAL EANIAVFAAY PGTPSSELTD
60 70 80 90 100
TMAAVAKKAG VYMEYSTNEK VAFETALAAA WSGLRAMTAM KHVGLNVAAD
110 120 130 140 150
SFLSSVGMGV EGGFVIMVAD DPSMWSSQNE QDTRVYAKFA NVPVLEPSSP
160 170 180 190 200
HEAKEMTKYA FELSEKFKHF VILRTTTRSS HARGDVVLGE LPEEIKTGKR
210 220 230 240 250
KFGKFKKDPT RFVDVPAHAR KFHPLILEKI EKIREELNNC PFNWIEGKED
260 270 280 290 300
AKVGIIAPGL SYAYVKEALA WLGVEDVKIL KLGTPFPVPY GLLGKFFDGL
310 320 330 340 350
EKVLIVEELE PVVEEQVKTW AYDKGLRIPI HGKDLVPRVY EMTTRRAVEA
360 370 380 390 400
IAKFLGLETP INFAEIDEKY EKVSQIVPPR PPSLCPACPH RNSFFAIRKA
410 420 430 440 450
AGPKAIYPSD IGCYTLGVLP PLRTVDTTVA MGASIGIGHG LSIAMNGSLA
460 470 480 490 500
EEEHKEGKEK QIIVATIGDS TFYHTGLPAL ANAIYNRSNV LIVVLDNLVT
510 520 530 540 550
AMTGDQPNPG TGQTPHGMGK RIPIEDVAKA MGADFVAVVD PYDIKATYET
560 570 580 590 600
IKKALEVEGV SVVVSRQVCA LYKIGQMRRR GMKWPIYHVV EDKCTGCKIC
610 620 630 640
INAYGCPAIY WDPETKKAKV DPTMCWGCGG CAQVCPFDAF EPMKEGE
Length:647
Mass (Da):70,900
Last modified:March 29, 2005 - v2
Checksum:iAE24D283C96CDBF7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261A → E in BAA20528 (PubMed:9180697).Curated
Sequence conflicti50 – 545DTMAA → IRWQT in BAA20528 (PubMed:9180697).Curated
Sequence conflicti311 – 3111P → R in BAA20528 (PubMed:9180697).Curated
Sequence conflicti602 – 6032NA → KT in BAA20528 (PubMed:9180697).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86221 Genomic DNA. Translation: BAA20528.1.
AP006878 Genomic DNA. Translation: BAD84325.1.
RefSeqiWP_011249091.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD84325; BAD84325; TK0136.
GeneIDi3235682.
KEGGitko:TK0136.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86221 Genomic DNA. Translation: BAA20528.1.
AP006878 Genomic DNA. Translation: BAD84325.1.
RefSeqiWP_011249091.1. NC_006624.1.

3D structure databases

ProteinModelPortaliO07835.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi69014.TK0136.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD84325; BAD84325; TK0136.
GeneIDi3235682.
KEGGitko:TK0136.

Phylogenomic databases

eggNOGiarCOG01609. Archaea.
COG4231. LUCA.
HOGENOMiHOG000224871.
InParanoidiO07835.
KOiK00179.
OMAiMMPAMAR.

Enzyme and pathway databases

BioCyciTKOD69014:GH72-138-MONOMER.
BRENDAi1.2.7.8. 5246.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017721. Indolepyruvate_Fd_OxRdtase_asu.
IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR029061. THDP-binding.
IPR011766. TPP_enzyme-bd_C.
IPR009014. Transketo_C/PFOR_II.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
PF01855. POR_N. 1 hit.
PF02775. TPP_enzyme_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006439. Indolepyruvate_ferr_oxidored. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
TIGRFAMsiTIGR03336. IOR_alpha. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Indolepyruvate ferredoxin oxidoreductase from Pyrococcus sp. KOD1 possesses a mosaic structure showing features of various oxidoreductases."
    Siddiqui M.A., Fujiwara S., Imanaka T.
    Mol. Gen. Genet. 254:433-439(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PRESENCE OF A 3FE-4S AND TWO 4FE-4S CLUSTERS.
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.
  2. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiIORA_THEKO
AccessioniPrimary (citable) accession number: O07835
Secondary accession number(s): Q5JFI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 29, 2005
Last modified: April 13, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.