O07835 (IORA_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Indolepyruvate oxidoreductase subunit iorA
Short name=IOR
EC=1.2.7.8

Alternative name(s):
Indolepyruvate ferredoxin oxidoreductase subunit alpha

Gene names

Name:	iorA
Ordered Locus Names:	TK0136

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.
Catalytic activity	(Indol-3-yl)pyruvate + CoA + 2 oxidized ferredoxin = S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+.
Cofactor	Binds 1 4Fe-4S cluster.
Subunit structure	Heterodimer of the iorA and iorB subunits.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 70 degrees Celsius.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro indolepyruvate ferredoxin oxidoreductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 647	647	Indolepyruvate oxidoreductase subunit iorA		PRO_0000099930
Regions
	Domain	585 – 614	30	4Fe-4S ferredoxin-type 1
	Domain	616 – 645	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	625	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	628	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	631	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	635	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	26	1	A → E in BAA20528. Ref.1
	Sequence conflict	50 – 54	5	DTMAA → IRWQT in BAA20528. Ref.1
	Sequence conflict	311	1	P → R in BAA20528. Ref.1
	Sequence conflict	602 – 603	2	NA → KT in BAA20528. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O07835 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: AE24D283C96CDBF7	FASTA	647	70,900
	10 20 30 40 50 60 MAKVTDIVLW DKPGERVLLL GNHAIARGAL EANIAVFAAY PGTPSSELTD TMAAVAKKAG 70 80 90 100 110 120 VYMEYSTNEK VAFETALAAA WSGLRAMTAM KHVGLNVAAD SFLSSVGMGV EGGFVIMVAD 130 140 150 160 170 180 DPSMWSSQNE QDTRVYAKFA NVPVLEPSSP HEAKEMTKYA FELSEKFKHF VILRTTTRSS 190 200 210 220 230 240 HARGDVVLGE LPEEIKTGKR KFGKFKKDPT RFVDVPAHAR KFHPLILEKI EKIREELNNC 250 260 270 280 290 300 PFNWIEGKED AKVGIIAPGL SYAYVKEALA WLGVEDVKIL KLGTPFPVPY GLLGKFFDGL 310 320 330 340 350 360 EKVLIVEELE PVVEEQVKTW AYDKGLRIPI HGKDLVPRVY EMTTRRAVEA IAKFLGLETP 370 380 390 400 410 420 INFAEIDEKY EKVSQIVPPR PPSLCPACPH RNSFFAIRKA AGPKAIYPSD IGCYTLGVLP 430 440 450 460 470 480 PLRTVDTTVA MGASIGIGHG LSIAMNGSLA EEEHKEGKEK QIIVATIGDS TFYHTGLPAL 490 500 510 520 530 540 ANAIYNRSNV LIVVLDNLVT AMTGDQPNPG TGQTPHGMGK RIPIEDVAKA MGADFVAVVD 550 560 570 580 590 600 PYDIKATYET IKKALEVEGV SVVVSRQVCA LYKIGQMRRR GMKWPIYHVV EDKCTGCKIC 610 620 630 640 INAYGCPAIY WDPETKKAKV DPTMCWGCGG CAQVCPFDAF EPMKEGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Indolepyruvate ferredoxin oxidoreductase from Pyrococcus sp. KOD1 possesses a mosaic structure showing features of various oxidoreductases." Siddiqui M.A., Fujiwara S., Imanaka T. Mol. Gen. Genet. 254:433-439(1997) [PubMed: 9180697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.
[2]	"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86221 Genomic DNA. Translation: BAA20528.1. AP006878 Genomic DNA. Translation: BAD84325.1.
RefSeq	YP_182549.1. NC_006624.1.
3D structure databases
ProteinModelPortal	O07835.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000006287; EBPYRP00000006068; EBPYRG00000006286.
GeneID	3235682.
GenomeReviews	Gene locus TK0136 in contig AP006878_GR.
KEGG	tko:TK0136.
NMPDR	fig|69014.3.peg.304.
Phylogenomic databases
eggNOG	arNOG04233.
GeneTree	EBGT00050000022252.
HOGENOM	HBG733885.
OMA	VADDPSM.
ProtClustDB	CLSK785560.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0136-MONOMER.
Family and domain databases
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR017721. Indolepyruvate_Fd_OxRdtase_asu. IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. [Graphical view]
KO	K00179.
Pfam	PF00037. Fer4. 1 hit. PF01855. POR_N. 1 hit. PF02775. TPP_enzyme_C. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR03336. IOR_alpha. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IORA_PYRKO

Accession

Primary (citable) accession number: O07835
Secondary accession number(s): Q5JFI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	March 29, 2005
Last modified:	November 16, 2011
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program