O07825 (NDOC_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Naphthalene 1,2-dioxygenase subunit beta
EC=1.14.12.12

Alternative name(s):
Naphthalene 1,2-dioxygenase ISP beta

Gene names

Name:	ndoC
Synonyms:	ndoC3

Encoded on

Plasmid

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	158 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-naphthalene dihydrodiol. The beta subunit may be responsible for the substrate specificity of the enzyme.
Catalytic activity	Naphthalene + NADH + O2 = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+.
Cofactor	Binds 1 2Fe-2S cluster Probable. Binds 1 iron ion Probable.
Pathway	Aromatic compound metabolism; naphthalene degradation.
Subunit structure	Naphthalene dioxygenase (NDO) multicomponent enzyme system is composed of an electron transfer component and an iron sulfur protein (ISP). The electron transfer component is composed of ferredoxin reductase (ndoR) and ferredoxin (ndoA), and ISP is composed of a large alpha subunit (ndoB) and a small beta subunit (ndoC).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	naphthalene 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›158	›158	Naphthalene 1,2-dioxygenase subunit beta		PRO_0000085075
Experimental info
	Non-terminal residue	158	1

Sequences

	Sequence		Length	Mass (Da)
	O07825 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 9C80BB72C22E7EF9	FASTA	158	18,597
	10 20 30 40 50 60 MMINTQEDKL VSAHDAEEFL RFFNCHDSAL QQEATTLLTR EAHLLDIQAY RTWLEHCVGS 70 80 90 100 110 120 EVQYQVISRE LRAASERRYK LNEAMNVYNE NFQQLKVRVE HQLDSQNWSN SPKLRFTRFI 130 140 150 TNVQAAMDVN DEDLLHVRSN VVLHRARRGN QVDVFYAA

References

[1]	"Naphthalene dioxygenase genes from Pseudomonas fluorescens." Hamann C. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17483 / DSM 6506 / JCM 6159 / Stanier 109.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF004283 Genomic DNA. Translation: AAB61371.1.
3D structure databases
ProteinModelPortal	O07825.
SMR	O07825. Positions 2-158.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000391. Rng_hydr_dOase-bsu. [Graphical view]
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDOC_PSEFL

Accession

Primary (citable) accession number: O07825

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	June 28, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program