ID   NDOB_PSEFL              Reviewed;         449 AA.
AC   O07824;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   16-JUN-2009, entry version 55.
DE   RecName: Full=Naphthalene 1,2-dioxygenase subunit alpha;
DE            EC=1.14.12.12;
DE   AltName: Full=Naphthalene 1,2-dioxygenase ISP alpha;
GN   Name=ndoB; Synonyms=ndoC2;
OS   Pseudomonas fluorescens.
OG   Plasmid.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 17483 / DSM 6506 / JCM 6159 / Stanier 109;
RA   Hamann C.;
RT   "Naphthalene dioxygenase genes from Pseudomonas fluorescens.";
RL   Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the naphthalene dioxygenase (NDO)
CC       multicomponent enzyme system which catalyzes the incorporation of
CC       both atoms of molecular oxygen into naphthalene to form cis-
CC       naphthalene dihydrodiol.
CC   -!- CATALYTIC ACTIVITY: Naphthalene + NADH + O(2) = (1R,2S)-1,2-
CC       dihydronaphthalene-1,2-diol + NAD(+).
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster (Probable).
CC   -!- COFACTOR: Binds 1 iron ion (Probable).
CC   -!- PATHWAY: Aromatic compound metabolism; naphtalene degradation.
CC   -!- SUBUNIT: Naphthalene dioxygenase (NDO) multicomponent enzyme
CC       system is composed of an electron transfer component and an iron
CC       sulfur protein (ISP). The electron transfer component is composed
CC       of ferredoxin reductase (ndoR) and ferredoxin (ndoA), and ISP is
CC       composed of a large alpha subunit (ndoB) and a small beta subunit
CC       (ndoC).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase alpha subunit family.
CC   -!- SIMILARITY: Contains 1 Rieske domain.
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DR   EMBL; AF004283; AAB61370.1; -; Genomic_DNA.
DR   HSSP; P23094; 1O7N.
DR   SMR; O07824; 1-447.
DR   BRENDA; 1.14.12.12; 329.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0018625; F:naphthalene 1,2-dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR015881; Ring-hydroxy_dOase_2Fe2S_BS.
DR   InterPro; IPR015879; Ring_hydroxy_dOase_asu_C.
DR   InterPro; IPR001663; Rng_hydr_dOase-A.
DR   Gene3D; G3DSA:2.102.10.10; Rieske_reg; 1.
DR   PANTHER; PTHR21266:SF2; Rng_hydr_dOase-A; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   Pfam; PF00848; Ring_hydroxyl_A; 1.
DR   PRINTS; PR00090; RNGDIOXGNASE.
DR   PROSITE; PS51296; RIESKE; 1.
DR   PROSITE; PS00570; RING_HYDROXYL_ALPHA; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Aromatic hydrocarbons catabolism; Dioxygenase; Iron;
KW   Iron-sulfur; Metal-binding; NAD; Oxidoreductase; Plasmid.
FT   CHAIN         1    449       Naphthalene 1,2-dioxygenase subunit
FT                                alpha.
FT                                /FTId=PRO_0000085055.
FT   DOMAIN       39    137       Rieske.
FT   METAL        81     81       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL        83     83       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   METAL       101    101       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL       104    104       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   METAL       208    208       Iron (By similarity).
FT   METAL       213    213       Iron (By similarity).
FT   METAL       362    362       Iron (By similarity).
SQ   SEQUENCE   449 AA;  49514 MW;  186A7924AD437A1C CRC64;
     MNYKNKILVS ESGLTQKHLI HGDEELFQHE LRTIXARNWL FLTHDSLIPS PGDYVTAKMG
     IDEVIVSRQS DGSIRAFLNV CRHRGKTLVN AEAGNAKGFV CSYHGWGFGS NGELQSVPFE
     KELYGESLNK KCLGLKEVAR VESFHGFIYG CFDQEAPSLM DYLGDAAWYL EPIFKHSGGL
     ELVGPPGKVV IKANWKAPAE NFVGDAYHVG WTHASSLRTG ESIFSSLAGN AVLPPEGAGL
     QMTSKYGSGM GVLWDGYSGV HSADLVPELM AFGGAKQERL NKEIGDVPAR IYRSHLNCTV
     FPNNSVLTCS GVFKVWNPID ANTTEVWTYA IVEKDMPEDL KRRLADAVQR TFGPAGFWES
     DDNDNMETAS QNGKKYQSRD SDLISNLGFG KDVYGDAVYP GVVGKSAIGE TSYRGFYRAY
     QAHVSSSNWA EFEDASSTWH TELTKTTDR
//