O07824 (NDOB_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Naphthalene 1,2-dioxygenase subunit alpha
EC=1.14.12.12

Alternative name(s):
Naphthalene 1,2-dioxygenase ISP alpha

Gene names

Name:	ndoB
Synonyms:	ndoC2

Encoded on

Plasmid

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-naphthalene dihydrodiol.
Catalytic activity	Naphthalene + NADH + O2 = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+.
Cofactor	Binds 1 2Fe-2S cluster Probable. Binds 1 iron ion Probable.
Pathway	Aromatic compound metabolism; naphthalene degradation.
Subunit structure	Naphthalene dioxygenase (NDO) multicomponent enzyme system is composed of an electron transfer component and an iron sulfur protein (ISP). The electron transfer component is composed of ferredoxin reductase (NdoR) and ferredoxin (NdoA), and ISP is composed of a large alpha subunit (NdoB) and a small beta subunit (NdoC).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro naphthalene 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Naphthalene 1,2-dioxygenase subunit alpha		PRO_0000085055
Regions
	Domain	39 – 137	99	Rieske
Sites
	Metal binding	81	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	83	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	101	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	104	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	208	1	Iron By similarity
	Metal binding	213	1	Iron By similarity
	Metal binding	362	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O07824 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 186A7924AD437A1C	FASTA	449	49,514
	10 20 30 40 50 60 MNYKNKILVS ESGLTQKHLI HGDEELFQHE LRTIXARNWL FLTHDSLIPS PGDYVTAKMG 70 80 90 100 110 120 IDEVIVSRQS DGSIRAFLNV CRHRGKTLVN AEAGNAKGFV CSYHGWGFGS NGELQSVPFE 130 140 150 160 170 180 KELYGESLNK KCLGLKEVAR VESFHGFIYG CFDQEAPSLM DYLGDAAWYL EPIFKHSGGL 190 200 210 220 230 240 ELVGPPGKVV IKANWKAPAE NFVGDAYHVG WTHASSLRTG ESIFSSLAGN AVLPPEGAGL 250 260 270 280 290 300 QMTSKYGSGM GVLWDGYSGV HSADLVPELM AFGGAKQERL NKEIGDVPAR IYRSHLNCTV 310 320 330 340 350 360 FPNNSVLTCS GVFKVWNPID ANTTEVWTYA IVEKDMPEDL KRRLADAVQR TFGPAGFWES 370 380 390 400 410 420 DDNDNMETAS QNGKKYQSRD SDLISNLGFG KDVYGDAVYP GVVGKSAIGE TSYRGFYRAY 430 440 QAHVSSSNWA EFEDASSTWH TELTKTTDR

References

[1]	"Naphthalene dioxygenase genes from Pseudomonas fluorescens." Hamann C. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17483 / DSM 6506 / JCM 6159 / Stanier 109.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF004283 Genomic DNA. Translation: AAB61370.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00082.
Family and domain databases
Gene3D	2.102.10.10. 1 hit.
InterPro	IPR017941. Rieske_2Fe-2S. IPR015881. Ring-hydroxy_dOase_2Fe2S_BS. IPR015879. Ring_hydroxy_dOase_asu_C_dom. IPR001663. Rng_hydr_dOase-A. [Graphical view]
Pfam	PF00355. Rieske. 1 hit. PF00848. Ring_hydroxyl_A. 1 hit. [Graphical view]
PRINTS	PR00090. RNGDIOXGNASE.
SUPFAM	SSF50022. Rieske_dom. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS00570. RING_HYDROXYL_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDOB_PSEFL

Accession

Primary (citable) accession number: O07824

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	March 6, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program