O07684 (BGAL_LACAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase large subunit
Short name=Beta-gal large subunit
EC=3.2.1.23

Alternative name(s):
Lactase large subunit

Gene names

Name:	lacL
Ordered Locus Names:	LBA1467

Organism

Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]

Taxonomic identifier

272621 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subunit structure	Heterodimer of a large and a small subunit.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	Beta-galactosidase large subunit		PRO_0000057665
Sites
	Active site	468	1	Proton donor By similarity
	Active site	536	1	Nucleophile By similarity
Experimental info
	Sequence conflict	302	1	H → N in BAA20536. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O07684 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: B73F7C691CC6B7E6	FASTA	628	73,254
	10 20 30 40 50 60 MQANIKWLDD PEIFRVNQLP AHSDHPFFKN YREWQNNHSS FKQSLNGMWQ FKFSKDPQSR 70 80 90 100 110 120 PVDFYKKDFN TSSFTTIPVP SEIELNNFAQ NQYINTLYPW EGKIFRRPAY ALNKSDAEEG 130 140 150 160 170 180 SFSEGKDNTV GSYIKHFDLN PELRDHDIHI VFEGAERAMY VWLNGHFIGY AEDSFTPSEF 190 200 210 220 230 240 DLTKYIKEKD NILAVEVFKH STASWLEDQD MFRFSGLFRS VELLAFPETH LVDLDLKPTV 250 260 270 280 290 300 CDNYQDGIFN AELKFTGSLN GHVHLSVEDV NGSAILEQDV PLDSEVEFTS STLENIHLWD 310 320 330 340 350 360 NHHPYLYQLL IEVHDENGHL VELIPYQFGF RRIEINQDKV ILLNGKCLII NGVNRHEWNA 370 380 390 400 410 420 KTGRSITLND MEKDIDTFKE NNINAVRTCH YPNQIPWYYL CDQNGIYMMA ENNLESHGTW 430 440 450 460 470 480 QKMGQVEPSD NVPGSVPEWR EAVIDRARNN YETFKNHTSI LFWSLGNESY AGSNIVAMNE 490 500 510 520 530 540 FYKEHDSSRL VHYEGVVHRP ELKDQISDIE SHMYLPPKEV AEYLRNNPQK PFMECEYMHD 550 560 570 580 590 600 MGNSDGGMGS YIKLIDEYPQ YVGGFIWDFI DQALLVYDPI SKQNVLRYGG DFDDRHSDYE 610 620 FSGDGLMFAD RTPKPAMQEV RYYYGLHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Suzuki M., Saito T., Konno K., Kamio Y., Itoh T. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JCM 1229 / KCTC 3168.
[2]	"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed: 15671160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB004867 Genomic DNA. Translation: BAA20536.1. CP000033 Genomic DNA. Translation: AAV43287.1.
RefSeq	YP_194318.1. NC_006814.3.
3D structure databases
ProteinModelPortal	O07684.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3251483.
GenomeReviews	Gene locus LBA1467 in contig CP000033_GR.
KEGG	lac:LBA1467.
NMPDR	fig|272621.3.peg.1379.
PATRIC	22197950. VBILacAci7974_1388.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG427253.
OMA	HIELAGY.
ProtClustDB	CLSK912517.
Enzyme and pathway databases
BioCyc	LACI272621:LBA1467-MONOMER.
BRENDA	3.2.1.23. 2846.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR023232. Glyco_hydro_2_AS. IPR023230. Glyco_hydro_2_CS. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01190.
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_LACAC

Accession

Primary (citable) accession number: O07684
Secondary accession number(s): Q5FJ37

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	March 29, 2005
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program