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O07684 (BGAL_LACAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase large subunit

Short name=Beta-gal large subunit
EC=3.2.1.23
Alternative name(s):
Lactase large subunit
Gene names
Name:lacL
Ordered Locus Names:LBA1467
OrganismLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]
Taxonomic identifier272621 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length628 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subunit structure

Heterodimer of a large and a small subunit.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 628628Beta-galactosidase large subunit
PRO_0000057665

Sites

Active site4681Proton donor By similarity
Active site5361Nucleophile By similarity

Experimental info

Sequence conflict3021H → N in BAA20536. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O07684 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: B73F7C691CC6B7E6

FASTA62873,254
        10         20         30         40         50         60 
MQANIKWLDD PEIFRVNQLP AHSDHPFFKN YREWQNNHSS FKQSLNGMWQ FKFSKDPQSR 

        70         80         90        100        110        120 
PVDFYKKDFN TSSFTTIPVP SEIELNNFAQ NQYINTLYPW EGKIFRRPAY ALNKSDAEEG 

       130        140        150        160        170        180 
SFSEGKDNTV GSYIKHFDLN PELRDHDIHI VFEGAERAMY VWLNGHFIGY AEDSFTPSEF 

       190        200        210        220        230        240 
DLTKYIKEKD NILAVEVFKH STASWLEDQD MFRFSGLFRS VELLAFPETH LVDLDLKPTV 

       250        260        270        280        290        300 
CDNYQDGIFN AELKFTGSLN GHVHLSVEDV NGSAILEQDV PLDSEVEFTS STLENIHLWD 

       310        320        330        340        350        360 
NHHPYLYQLL IEVHDENGHL VELIPYQFGF RRIEINQDKV ILLNGKCLII NGVNRHEWNA 

       370        380        390        400        410        420 
KTGRSITLND MEKDIDTFKE NNINAVRTCH YPNQIPWYYL CDQNGIYMMA ENNLESHGTW 

       430        440        450        460        470        480 
QKMGQVEPSD NVPGSVPEWR EAVIDRARNN YETFKNHTSI LFWSLGNESY AGSNIVAMNE 

       490        500        510        520        530        540 
FYKEHDSSRL VHYEGVVHRP ELKDQISDIE SHMYLPPKEV AEYLRNNPQK PFMECEYMHD 

       550        560        570        580        590        600 
MGNSDGGMGS YIKLIDEYPQ YVGGFIWDFI DQALLVYDPI SKQNVLRYGG DFDDRHSDYE 

       610        620 
FSGDGLMFAD RTPKPAMQEV RYYYGLHK 

« Hide

References

« Hide 'large scale' references
[1]Suzuki M., Saito T., Konno K., Kamio Y., Itoh T.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: JCM 1229 / KCTC 3168.
[2]"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM."
Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R.
Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB004867 Genomic DNA. Translation: BAA20536.1.
CP000033 Genomic DNA. Translation: AAV43287.1.
RefSeqYP_194318.1. NC_006814.3.

3D structure databases

ProteinModelPortalO07684.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272621.LBA1467.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV43287; AAV43287; LBA1467.
GeneID3251483.
KEGGlac:LBA1467.
PATRIC22197950. VBILacAci7974_1388.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000111236.
KOK01190.
OMAPYQFGFR.
OrthoDBEOG6XWV0T.

Enzyme and pathway databases

BRENDA3.2.1.23. 2846.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SUPFAMSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_LACAC
AccessionPrimary (citable) accession number: O07684
Secondary accession number(s): Q5FJ37
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: March 29, 2005
Last modified: May 14, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries