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Protein

Beta-galactosidase large subunit

Gene

lacL

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei468 – 4681Proton donorBy similarity
Active sitei536 – 5361NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.23. 2846.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase large subunit (EC:3.2.1.23)
Short name:
Beta-gal large subunit
Alternative name(s):
Lactase large subunit
Gene namesi
Name:lacL
Ordered Locus Names:LBA1467
OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Taxonomic identifieri272621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000006381: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 628628Beta-galactosidase large subunitPRO_0000057665Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Protein-protein interaction databases

STRINGi272621.LBA1467.

Structurei

3D structure databases

ProteinModelPortaliO07684.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000111236.
KOiK01190.
OMAiPYQFGFR.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O07684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQANIKWLDD PEIFRVNQLP AHSDHPFFKN YREWQNNHSS FKQSLNGMWQ
60 70 80 90 100
FKFSKDPQSR PVDFYKKDFN TSSFTTIPVP SEIELNNFAQ NQYINTLYPW
110 120 130 140 150
EGKIFRRPAY ALNKSDAEEG SFSEGKDNTV GSYIKHFDLN PELRDHDIHI
160 170 180 190 200
VFEGAERAMY VWLNGHFIGY AEDSFTPSEF DLTKYIKEKD NILAVEVFKH
210 220 230 240 250
STASWLEDQD MFRFSGLFRS VELLAFPETH LVDLDLKPTV CDNYQDGIFN
260 270 280 290 300
AELKFTGSLN GHVHLSVEDV NGSAILEQDV PLDSEVEFTS STLENIHLWD
310 320 330 340 350
NHHPYLYQLL IEVHDENGHL VELIPYQFGF RRIEINQDKV ILLNGKCLII
360 370 380 390 400
NGVNRHEWNA KTGRSITLND MEKDIDTFKE NNINAVRTCH YPNQIPWYYL
410 420 430 440 450
CDQNGIYMMA ENNLESHGTW QKMGQVEPSD NVPGSVPEWR EAVIDRARNN
460 470 480 490 500
YETFKNHTSI LFWSLGNESY AGSNIVAMNE FYKEHDSSRL VHYEGVVHRP
510 520 530 540 550
ELKDQISDIE SHMYLPPKEV AEYLRNNPQK PFMECEYMHD MGNSDGGMGS
560 570 580 590 600
YIKLIDEYPQ YVGGFIWDFI DQALLVYDPI SKQNVLRYGG DFDDRHSDYE
610 620
FSGDGLMFAD RTPKPAMQEV RYYYGLHK
Length:628
Mass (Da):73,254
Last modified:March 29, 2005 - v2
Checksum:iB73F7C691CC6B7E6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021H → N in BAA20536 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004867 Genomic DNA. Translation: BAA20536.1.
CP000033 Genomic DNA. Translation: AAV43287.1.
RefSeqiYP_194318.1. NC_006814.3.

Genome annotation databases

EnsemblBacteriaiAAV43287; AAV43287; LBA1467.
GeneIDi3251483.
KEGGilac:LBA1467.
PATRICi22197950. VBILacAci7974_1388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004867 Genomic DNA. Translation: BAA20536.1.
CP000033 Genomic DNA. Translation: AAV43287.1.
RefSeqiYP_194318.1. NC_006814.3.

3D structure databases

ProteinModelPortaliO07684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272621.LBA1467.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV43287; AAV43287; LBA1467.
GeneIDi3251483.
KEGGilac:LBA1467.
PATRICi22197950. VBILacAci7974_1388.

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000111236.
KOiK01190.
OMAiPYQFGFR.
OrthoDBiEOG6XWV0T.

Enzyme and pathway databases

BRENDAi3.2.1.23. 2846.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Suzuki M., Saito T., Konno K., Kamio Y., Itoh T.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JCM 1229 / KCTC 3168.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Entry informationi

Entry nameiBGAL_LACAC
AccessioniPrimary (citable) accession number: O07684
Secondary accession number(s): Q5FJ37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: March 29, 2005
Last modified: January 7, 2015
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.