O07653 (O07653_9GAMM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Cellulase B EMBL AAB61462.2

Gene names

Name:

celB EMBL AAB61462.2

Organism

Cellvibrio mixtus EMBL AAB61462.2

Taxonomic identifier

39650 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Cellvibrio

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UZ0 Metal-binding PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UZ0
Technical term	3D-structure PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UXZ PDB 1UZ0
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	498	1	Calcium 1 PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UZ0
	Metal binding	500	1	Calcium 1 PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UZ0
	Metal binding	507	1	Calcium 2; via carbonyl oxygen PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UZ0
	Metal binding	520	1	Calcium 1; via carbonyl oxygen PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UZ0
	Metal binding	524	1	Calcium 2; via carbonyl oxygen PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UZ0
	Metal binding	526	1	Calcium 2 PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UZ0
	Metal binding	529	1	Calcium 2 PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UZ0
	Metal binding	614	1	Calcium 1 PDB 1UYX PDB 1UYY PDB 1UYZ PDB 1UY0 PDB 1UZ0

Sequences

	Sequence		Length	Mass (Da)
	O07653 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: CE917EB6D97CE57C	FASTA	622	65,889
	10 20 30 40 50 60 MKNNHPGCAA PTAARSTNPR NRLFASLVTG LASIAFCSTA LAAVPALQVQ GNKVLVGGKS 70 80 90 100 110 120 VSLEGVSLFW SNTGWGAEKF YTAAAVKRAK TEFNANLIRA AIGHGEGGGI QDDWNGNMAR 130 140 150 160 170 180 LDTVIQAAID NDMYVIVDYH SHKAHENWGS AEAFFKQVAQ KWGQHNNVIY ELYNEPLGVD 190 200 210 220 230 240 WHSVLKPYAE HVGGKIRAID PDNLIIMGTP NWSQDVDVAS TNKANVSNLA YTVHFYADSH 250 260 270 280 290 300 RGSYRAKAQT ALNNGAALFA TEWGVGHANG RGTVNHAETW AWIDFLRANG ISHAGWAFHD 310 320 330 340 350 360 KERDQATGEV ETSSFFWADG SLKESGRFVK EILAGRKPID GGGGDGGGDG SGGTCTKANP 370 380 390 400 410 420 GDAIQAESWC QMSGVKTETT SDAGDGLNVG YIDGGDWMTY SVNIPTTGTY KVSYRVAAQA 430 440 450 460 470 480 GGGQLQLEKA GGSPVYSNIN VPATGGWQNW QTISHNVVLP AGEQLIALSA ITGGFNINWL 490 500 510 520 530 540 KVESTGGDNS GTVIATIQAE DHSQQSGTQQ ETTTDTGGGK NVGYIDAGDW LSYAGTPVNI 550 560 570 580 590 600 PSSGSYLIEY RVASQNGGGS LTFEEAGGAP VHGTIAIPAT GGWQTWTTIQ HTVNLSAGSH 610 620 QFGIKANAGG WNLNWIRINK TH

References

[1]	"Gene sequence and properties of CelB, a modular cellulase from the aerobic soil bacterium Cellvibrio mixtus." Fontes C.M.G.A., Clarke J.H., Hazlewood G.P., Fernandes T.H., Gilbert H.J., Ferreira L.M.A. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5a in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities." Pires V.M., Henshaw J.L., Prates J.A., Bolam D.N., Ferreira L.M., Fontes C.M., Henrissat B., Planas A., Gilbert H.J., Czjzek M. J. Biol. Chem. 279:21560-21568(2004) [PubMed: 15010454] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 493-622 IN COMPLEX WITH CALCIUM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF003697 Genomic DNA. Translation: AAB61462.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UXZ	X-ray	1.40	A/B	493-622	[»]
1UY0	X-ray	1.65	A/B	493-622	[»]
1UYX	X-ray	1.47	A/B	493-622	[»]
1UYY	X-ray	1.47	A/B	493-622	[»]
1UYZ	X-ray	1.60	A/B	493-622	[»]
1UZ0	X-ray	2.00	A	493-622	[»]

ProteinModelPortal

O07653.

SMR

O07653. Positions 43-335, 493-622.

ModBase

Search...

Protein family/group databases

CAZy

CBM6. Carbohydrate-Binding Module Family 6.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR006584. Cellulose-bd_IV.
IPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

Pfam

PF03422. CBM_6. 2 hits.
PF00150. Cellulase. 1 hit.
[Graphical view]

SMART

SM00606. CBD_IV. 2 hits.
[Graphical view]

SUPFAM

SSF49785. Gal_bind_like. 2 hits.
SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS51175. CBM6. 2 hits.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

O07653_9GAMM

Accession

Primary (citable) accession number: O07653

Entry history

Integrated into UniProtKB/TrEMBL:	July 1, 1997
Last sequence update:	June 1, 2002
Last modified:	May 31, 2011
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)