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Protein

Nitrogenase iron protein

Gene

nifH

Organism
Cyanothece sp. (strain ATCC 51142)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.By similarity

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per dimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi132Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1
Metal bindingi166Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron protein (EC:1.18.6.1)
Alternative name(s):
Nitrogenase Fe protein
Nitrogenase component II
Nitrogenase reductase
Gene namesi
Name:nifH
Ordered Locus Names:cce_0559
OrganismiCyanothece sp. (strain ATCC 51142)
Taxonomic identifieri43989 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesCyanothecaceaeCyanothece
Proteomesi
  • UP000001203 Componenti: Chromosome circular

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001395041 – 327Nitrogenase iron proteinAdd BLAST327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseBy similarity1

Post-translational modificationi

The reversible ADP-ribosylation of Arg-135 inactivates the nitrogenase reductase and regulates nitrogenase activity.By similarity

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi43989.cce_0559.

Structurei

3D structure databases

ProteinModelPortaliO07641.
SMRiO07641.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 37PEST-like; not found in other NifHAdd BLAST37

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.Curated

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228826.
KOiK02588.
OMAiYQGVKCV.
OrthoDBiPOG091H0230.

Family and domain databases

CDDicd02040. NifH. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O07641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRNSQGFLT TIRIIVTSAT DQPSNFIHSL SNKRESTMRQ IAFYGKGGIG
60 70 80 90 100
KSTTSQNTIA ALAETNRIMI VGCDPKADST RLMLHTKAQT TILHLAAERG
110 120 130 140 150
TVEDIELEEV LLEGYQGVKC VESGGPEPGV GCAGRGIITA INFLEEEGAY
160 170 180 190 200
EDLDFVSYDV LGDVVCGGFA MPIREGKAQE IYIVTSGEMM AMYAANNIAR
210 220 230 240 250
GILKYAHTGG VRLGGLICNS RNVNCEAELI EELARRLGTQ MIHFVPRSKQ
260 270 280 290 300
VQEAELRRMT VIEYSPDHPQ AQEYRELSRK IENNTNLVIP TPITMEELEE
310 320
LLVDFGILGG EDEYEKALQA DKAATKA
Length:327
Mass (Da):35,823
Last modified:July 1, 1997 - v1
Checksum:iD26946E425517D36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF003336 Genomic DNA. Translation: AAB61408.1.
CP000806 Genomic DNA. Translation: ACB49910.1.
RefSeqiWP_009546639.1. NC_010546.1.

Genome annotation databases

EnsemblBacteriaiACB49910; ACB49910; cce_0559.
KEGGicyt:cce_0559.
PATRICi21539586. VBICyaSp130209_0639.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF003336 Genomic DNA. Translation: AAB61408.1.
CP000806 Genomic DNA. Translation: ACB49910.1.
RefSeqiWP_009546639.1. NC_010546.1.

3D structure databases

ProteinModelPortaliO07641.
SMRiO07641.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi43989.cce_0559.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB49910; ACB49910; cce_0559.
KEGGicyt:cce_0559.
PATRICi21539586. VBICyaSp130209_0639.

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228826.
KOiK02588.
OMAiYQGVKCV.
OrthoDBiPOG091H0230.

Family and domain databases

CDDicd02040. NifH. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIFH_CYAA5
AccessioniPrimary (citable) accession number: O07641
Secondary accession number(s): B1WPC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.