Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutaminase 2

Gene

glsA2

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation1 Publication

Kineticsi

  1. KM=7.6 mM for glutamine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei65 – 651SubstrateUniRule annotation
    Binding sitei117 – 1171SubstrateUniRule annotation
    Binding sitei162 – 1621SubstrateUniRule annotation
    Binding sitei169 – 1691SubstrateUniRule annotation
    Binding sitei193 – 1931SubstrateUniRule annotation
    Binding sitei245 – 2451SubstrateUniRule annotation
    Binding sitei263 – 2631Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciBSUB:BSU14830-MONOMER.
    BRENDAi3.5.1.2. 658.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaminase 2UniRule annotation (EC:3.5.1.2UniRule annotation)
    Gene namesi
    Name:glsA2UniRule annotation
    Synonyms:ylaM
    Ordered Locus Names:BSU14830
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570 Componenti: Chromosome

    Organism-specific databases

    GenoListiBSU14830. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Glutaminase 2PRO_0000110595Add
    BLAST

    Proteomic databases

    PaxDbiO07637.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100008211.

    Structurei

    3D structure databases

    ProteinModelPortaliO07637.
    SMRiO07637. Positions 4-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutaminase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2066.
    HOGENOMiHOG000216890.
    InParanoidiO07637.
    KOiK01425.
    OMAiTKQCAIE.
    OrthoDBiEOG6N94BK.
    PhylomeDBiO07637.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O07637-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVCQHNDELE ALVKKAKKVT DKGEVASYIP ALAKADKHDL SVAIYYSNNV
    60 70 80 90 100
    CLSAGDVEKT FTLQSISKVL SLALVLMEYG KDKVFSYVGQ EPTGDPFNSI
    110 120 130 140 150
    IKLETVNPSK PLNPMINAGA LVVTSLIRGR TVKERLDYLL SFIRRLTNNQ
    160 170 180 190 200
    EITYCREVAE SEYSTSMINR AMCYYMKQYG IFEDDVEAVM DLYTKQCAIE
    210 220 230 240 250
    MNSLDLAKIG SVFALNGRHP ETGEQVISKD VARICKTFMV TCGMYNASGE
    260 270 280 290 300
    FAIKVGIPAK SGVSGGIMGI SPYDFGIGIF GPALDEKGNS IAGVKLLEIM

    SEMYRLSIF
    Length:309
    Mass (Da):34,012
    Last modified:July 1, 1997 - v1
    Checksum:iE1DFEF1A0723C1A5
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z97025 Genomic DNA. Translation: CAB09718.1.
    AL009126 Genomic DNA. Translation: CAB13356.1.
    PIRiC69873.
    RefSeqiNP_389366.1. NC_000964.3.
    WP_003245206.1. NZ_JNCM01000035.1.

    Genome annotation databases

    EnsemblBacteriaiCAB13356; CAB13356; BSU14830.
    GeneIDi939852.
    KEGGibsu:BSU14830.
    PATRICi18974763. VBIBacSub10457_1573.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z97025 Genomic DNA. Translation: CAB09718.1.
    AL009126 Genomic DNA. Translation: CAB13356.1.
    PIRiC69873.
    RefSeqiNP_389366.1. NC_000964.3.
    WP_003245206.1. NZ_JNCM01000035.1.

    3D structure databases

    ProteinModelPortaliO07637.
    SMRiO07637. Positions 4-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100008211.

    Proteomic databases

    PaxDbiO07637.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB13356; CAB13356; BSU14830.
    GeneIDi939852.
    KEGGibsu:BSU14830.
    PATRICi18974763. VBIBacSub10457_1573.

    Organism-specific databases

    GenoListiBSU14830. [Micado]

    Phylogenomic databases

    eggNOGiCOG2066.
    HOGENOMiHOG000216890.
    InParanoidiO07637.
    KOiK01425.
    OMAiTKQCAIE.
    OrthoDBiEOG6N94BK.
    PhylomeDBiO07637.

    Enzyme and pathway databases

    BioCyciBSUB:BSU14830-MONOMER.
    BRENDAi3.5.1.2. 658.

    Miscellaneous databases

    PROiO07637.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Bacillus subtilis chromosomal region downstream nprE."
      Purnelle B., Presecan E., Glaser P., Richou A., Danchin A., Goffeau A.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis."
      Brown G., Singer A., Proudfoot M., Skarina T., Kim Y., Chang C., Dementieva I., Kuznetsova E., Gonzalez C.F., Joachimiak A., Savchenko A., Yakunin A.F.
      Biochemistry 47:5724-5735(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

    Entry informationi

    Entry nameiGLSA2_BACSU
    AccessioniPrimary (citable) accession number: O07637
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 1, 1997
    Last modified: June 24, 2015
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.