O07623 (SBOA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Subtilosin-A

Alternative name(s):
Antilisterial bacteriocin subtilosin

Gene names

Name:	sboA
Synonyms:	sbo
Ordered Locus Names:	BSU37350

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	43 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has bacteriocidal activity against some Gram-positive bacteria such as Listeria, some species of Bacillus and E.faecium. Ref.5
Subcellular location	Secreted.
Developmental stage	The production of subtilosin A begins at the end of vegetative growth and finishes before spore formation.
Induction	Transcription is highly induced by oxygen limitation and is under dual and independent control of Spo0A-AbrB and ResDE. Ref.6
Post-translational modification	This peptide undergoes unique processing steps that include proteolytic cleavage after Glu-8, and covalent linkage of the alpha-amino of Asn-9 with the carboxyl of Gly-43 to form a cyclopeptide. Thioether cross-links are formed between cysteines and the alpha-carbons of other amino acids, Cys-12 to Phe-39, Cys-15 to Thr-36, and Cys-21 to Phe-30. In forming these cross-links, Thr-36 and Phe-39 are converted to D-amino-acids.
Sequence similarities	Belongs to the bacteriocin class V family.
Caution	Ref.4 sequence does not report residues in positions 30 and 39 probably due to their modification, and reports a cyclic permutation of the peptide sequence.
Mass spectrometry	Molecular mass is 3398.9 Da from positions 9 - 43. Determined by FAB. Ref.4

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Antibiotic Antimicrobial Bacteriocin
PTM	D-amino acid Thioether bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Propeptide

1 – 8

8

PRO_0000002780

Peptide

9 – 43

35

Subtilosin-A Ref.4

PRO_0000002781

Amino acid modifications

Cross-link

9 ↔ 43

Cyclopeptide (Asn-Gly)

Cross-link

12 ↔ 39

2-cysteinyl-D-phenylalanine (Cys-Phe)

Cross-link

15 ↔ 36

2-cysteinyl-D-allo-threonine (Cys-Thr)

Cross-link

21 ↔ 30

2-cysteinyl-L-phenylalanine (Cys-Phe)

Secondary structure

1

.

.

.

.

.

.

.

43

Helix Strand Turn

Details...

Turn

12 – 15

4

Turn

23 – 26

4

Helix

37 – 42

6

Sequences

	Sequence		Length	Mass (Da)
	O07623 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 055A81DA0D378794	FASTA	43	4,325
	10 20 30 40 MKKAVIVENK GCATCSIGAA CLVDGPIPDF EIAGATGLFG LWG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Subtilosin A biosynthesis is conserved among two different classes of Bacillus subtilis strains." Stein T., Duesterhus S., Entian K.-D. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6633 / PCI 219 / NRS 231.
[2]	"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)." Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P. Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Subtilosin A, a new antibiotic peptide produced by Bacillus subtilis 168: isolation, structural analysis, and biogenesis." Babasaki K., Takao T., Shimonishi Y., Kurahashi K. J. Biochem. 98:585-603(1985) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 9-43, CHARACTERIZATION, MASS SPECTROMETRY. Strain: 168.
[5]	"Genes of the sbo-alb locus of Bacillus subtilis are required for production of the antilisterial bacteriocin subtilosin." Zheng G., Yan L.Z., Vederas J.C., Zuber P. J. Bacteriol. 181:7346-7355(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: 168 / JH642 and 22a.
[6]	"Dual control of sbo-alb operon expression by the Spo0 and ResDE systems of signal transduction under anaerobic conditions in Bacillus subtilis." Nakano M.M., Zheng G., Zuber P. J. Bacteriol. 182:3274-3277(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION. Strain: 168 / JH642.
[7]	"Structure of subtilosin A, an antimicrobial peptide from Bacillus subtilis with unusual posttranslational modifications linking cysteine sulfurs to alpha-carbons of phenylalanine and threonine." Kawulka K., Sprules T., McKay R.T., Mercier P., Diaper C.M., Zuber P., Vederas J.C. J. Am. Chem. Soc. 125:4726-4727(2003) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, CROSS-LINKS CYS-PHE AND CYS-THR.
[8]	Vederas J.C. Unpublished observations (MAY-2003) Cited for: STEREOCHEMISTRY OF D-ALLO-THR-36.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ430547 Genomic DNA. Translation: CAD23198.1.
Z97024 Genomic DNA. Translation: CAB09701.1.
AL009126 Genomic DNA. Translation: CAB15763.1.

PIR

A69704.

RefSeq

NP_391616.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PXQ	NMR	-	A	9-43	[»]

ProteinModelPortal

O07623.

SMR

O07623. Positions 9-43.

ModBase

Search...

Protein-protein interaction databases

STRING

224308.BSU37350.

Protein family/group databases

TCDB

1.C.84.1.1. subtilosin family.

Proteomic databases

PaxDb

O07623.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB15763; CAB15763; BSU37350.

GeneID

938512.

KEGG

bsu:BSU37350.

PATRIC

18979508. VBIBacSub10457_3916.

Organism-specific databases

GenoList

BSU37350. [Micado]

Phylogenomic databases

ProtClustDB

CLSK2752544.

Enzyme and pathway databases

BioCyc

BSUB:BSU37350-MONOMER.

Family and domain databases

InterPro

IPR021539. Subtilosin_A.
[Graphical view]

Pfam

PF11420. Subtilosin_A. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O07623.

Entry information

Entry name

SBOA_BACSU

Accession

Primary (citable) accession number: O07623

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2003
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program