SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O07610

- LCFB_BACSU

UniProt

O07610 - LCFB_BACSU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Long-chain-fatty-acid--CoA ligase

Gene
lcfB, yhfL, BSU10270
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the degradation of long-chain fatty acids.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU10270-MONOMER.
RETL1328306-WGS:GSTH-5136-MONOMER.
RETL1328306-WGS:GSTH-936-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase
Gene namesi
Name:lcfB
Synonyms:yhfL
Ordered Locus Names:BSU10270
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU10270. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 513513Long-chain-fatty-acid--CoA ligasePRO_0000360675Add
BLAST

Proteomic databases

PaxDbiO07610.

Expressioni

Inductioni

Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to FadR boxes on target DNA and thus derepress transcription.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU10270.

Structurei

3D structure databases

ProteinModelPortaliO07610.
SMRiO07610. Positions 1-510.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0318.
HOGENOMiHOG000229983.
KOiK01897.
OrthoDBiEOG6MH5BV.
PhylomeDBiO07610.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020459. AMP-binding.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PRINTSiPR00154. AMPBINDING.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O07610-1 [UniParc]FASTAAdd to Basket

« Hide

MNLVSKLEET ASEKPDSIAC RFKDHMMTYQ ELNEYIQRFA DGLQEAGMEK    50
GDHLALLLGN SPDFIIAFFG ALKAGIVVVP INPLYTPTEI GYMLTNGDVK 100
AIVGVSQLLP LYESMHESLP KVELVILCQT GEAEPEAADP EVRMKMTTFA 150
KILRPTSAAK QNQEPVPDDT AVILYTSGTT GKPKGAMLTH QNLYSNANDV 200
AGYLGMDERD NVVCALPMFH VFCLTVCMNA PLMSGATVLI EPQFSPASVF 250
KLVKQQQATI FAGVPTMYNY LFQHENGKKD DFSSIRLCIS GGASMPVALL 300
TAFEEKFGVT ILEGYGLSEA SPVTCFNPFD RGRKPGSIGT SILHVENKVV 350
DPLGRELPAH QVGELIVKGP NVMKGYYKMP METEHALKDG WLYTGDLARR 400
DEDGYFYIVD RKKDMIIVGG YNVYPREVEE VLYSHPDVKE AVVIGVPDPQ 450
SGEAVKGYVV PKRSGVTEED IMQHCEKHLA KYKRPAAITF LDDIPKNATG 500
KMLRRALRDI LPQ 513
Length:513
Mass (Da):56,625
Last modified:June 16, 2009 - v2
Checksum:iD8BEBE46A9CB5183
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti219 – 2191F → C in CAA74533. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y14083 Genomic DNA. Translation: CAA74533.1.
AL009126 Genomic DNA. Translation: CAB12867.2.
PIRiA69831.
RefSeqiNP_388908.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB12867; CAB12867; BSU10270.
GeneIDi939308.
KEGGibsu:BSU10270.
PATRICi18973752. VBIBacSub10457_1070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y14083 Genomic DNA. Translation: CAA74533.1 .
AL009126 Genomic DNA. Translation: CAB12867.2 .
PIRi A69831.
RefSeqi NP_388908.2. NC_000964.3.

3D structure databases

ProteinModelPortali O07610.
SMRi O07610. Positions 1-510.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU10270.

Proteomic databases

PaxDbi O07610.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB12867 ; CAB12867 ; BSU10270 .
GeneIDi 939308.
KEGGi bsu:BSU10270.
PATRICi 18973752. VBIBacSub10457_1070.

Organism-specific databases

GenoListi BSU10270. [Micado ]

Phylogenomic databases

eggNOGi COG0318.
HOGENOMi HOG000229983.
KOi K01897.
OrthoDBi EOG6MH5BV.
PhylomeDBi O07610.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci BSUB:BSU10270-MONOMER.
RETL1328306-WGS:GSTH-5136-MONOMER.
RETL1328306-WGS:GSTH-936-MONOMER.

Family and domain databases

InterProi IPR025110. AMP-bd_C.
IPR020459. AMP-binding.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
PRINTSi PR00154. AMPBINDING.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene."
    Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S.
    Microbiology 144:859-875(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 219.
  4. "Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation."
    Matsuoka H., Hirooka K., Fujita Y.
    J. Biol. Chem. 282:5180-5194(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME, INDUCTION.
    Strain: 168.

Entry informationi

Entry nameiLCFB_BACSU
AccessioniPrimary (citable) accession number: O07610
Secondary accession number(s): Q796T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 16, 2009
Last modified: July 9, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi