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O07610 (LCFB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long-chain-fatty-acid--CoA ligase

EC=6.2.1.3
Alternative name(s):
Long-chain acyl-CoA synthetase
Gene names
Name:lcfB
Synonyms:yhfL
Ordered Locus Names:BSU10270
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the degradation of long-chain fatty acids.

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Pathway

Lipid metabolism; fatty acid beta-oxidation.

Induction

Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to FadR boxes on target DNA and thus derepress transcription. Ref.4

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
   Biological processFatty acid metabolism
Lipid degradation
Lipid metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfatty acid beta-oxidation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

long-chain fatty acid-CoA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 513513Long-chain-fatty-acid--CoA ligase
PRO_0000360675

Experimental info

Sequence conflict2191F → C in CAA74533. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O07610 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: D8BEBE46A9CB5183

FASTA51356,625
        10         20         30         40         50         60 
MNLVSKLEET ASEKPDSIAC RFKDHMMTYQ ELNEYIQRFA DGLQEAGMEK GDHLALLLGN 

        70         80         90        100        110        120 
SPDFIIAFFG ALKAGIVVVP INPLYTPTEI GYMLTNGDVK AIVGVSQLLP LYESMHESLP 

       130        140        150        160        170        180 
KVELVILCQT GEAEPEAADP EVRMKMTTFA KILRPTSAAK QNQEPVPDDT AVILYTSGTT 

       190        200        210        220        230        240 
GKPKGAMLTH QNLYSNANDV AGYLGMDERD NVVCALPMFH VFCLTVCMNA PLMSGATVLI 

       250        260        270        280        290        300 
EPQFSPASVF KLVKQQQATI FAGVPTMYNY LFQHENGKKD DFSSIRLCIS GGASMPVALL 

       310        320        330        340        350        360 
TAFEEKFGVT ILEGYGLSEA SPVTCFNPFD RGRKPGSIGT SILHVENKVV DPLGRELPAH 

       370        380        390        400        410        420 
QVGELIVKGP NVMKGYYKMP METEHALKDG WLYTGDLARR DEDGYFYIVD RKKDMIIVGG 

       430        440        450        460        470        480 
YNVYPREVEE VLYSHPDVKE AVVIGVPDPQ SGEAVKGYVV PKRSGVTEED IMQHCEKHLA 

       490        500        510 
KYKRPAAITF LDDIPKNATG KMLRRALRDI LPQ 

« Hide

References

« Hide 'large scale' references
[1]"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene."
Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S.
Microbiology 144:859-875(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 219.
[4]"Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation."
Matsuoka H., Hirooka K., Fujita Y.
J. Biol. Chem. 282:5180-5194(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE NAME, INDUCTION.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y14083 Genomic DNA. Translation: CAA74533.1.
AL009126 Genomic DNA. Translation: CAB12867.2.
PIRA69831.
RefSeqNP_388908.2. NC_000964.3.

3D structure databases

ProteinModelPortalO07610.
SMRO07610. Positions 1-510.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU10270.

Proteomic databases

PaxDbO07610.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12867; CAB12867; BSU10270.
GeneID939308.
KEGGbsu:BSU10270.
PATRIC18973752. VBIBacSub10457_1070.

Organism-specific databases

GenoListBSU10270. [Micado]

Phylogenomic databases

eggNOGCOG0318.
HOGENOMHOG000229983.
KOK01897.
OrthoDBEOG6MH5BV.
ProtClustDBPRK07656.

Enzyme and pathway databases

BioCycBSUB:BSU10270-MONOMER.
UniPathwayUPA00659.

Family and domain databases

InterProIPR025110. AMP-bd_C.
IPR020459. AMP-binding.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PRINTSPR00154. AMPBINDING.
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLCFB_BACSU
AccessionPrimary (citable) accession number: O07610
Secondary accession number(s): Q796T9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 16, 2009
Last modified: April 16, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList