Reviewed, UniProtKB/Swiss-Prot O07610 (LCFB_BACSU)

Names and origin

Protein names

Recommended name:
    Long-chain-fatty-acid--CoA ligase
    EC=6.2.1.3
Alternative name(s):
    Long-chain acyl-CoA synthetase

Gene names

Name:	lcfB
Synonyms:	yhfL
Ordered Locus Names:	BSU10270

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the degradation of long-chain fatty acids.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Induction	Repressed by fadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize fadR as to its binding to fadR boxes on target DNA and thus derepress transcription. Ref.4
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain-fatty-acid-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	Long-chain-fatty-acid--CoA ligase		PRO_0000360675
Experimental info
	Sequence conflict	219	1	F → C in CAA74533. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O07610-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: D8BEBE46A9CB5183	FASTA	513	56,625
	10 20 30 40 50 60 MNLVSKLEET ASEKPDSIAC RFKDHMMTYQ ELNEYIQRFA DGLQEAGMEK GDHLALLLGN 70 80 90 100 110 120 SPDFIIAFFG ALKAGIVVVP INPLYTPTEI GYMLTNGDVK AIVGVSQLLP LYESMHESLP 130 140 150 160 170 180 KVELVILCQT GEAEPEAADP EVRMKMTTFA KILRPTSAAK QNQEPVPDDT AVILYTSGTT 190 200 210 220 230 240 GKPKGAMLTH QNLYSNANDV AGYLGMDERD NVVCALPMFH VFCLTVCMNA PLMSGATVLI 250 260 270 280 290 300 EPQFSPASVF KLVKQQQATI FAGVPTMYNY LFQHENGKKD DFSSIRLCIS GGASMPVALL 310 320 330 340 350 360 TAFEEKFGVT ILEGYGLSEA SPVTCFNPFD RGRKPGSIGT SILHVENKVV DPLGRELPAH 370 380 390 400 410 420 QVGELIVKGP NVMKGYYKMP METEHALKDG WLYTGDLARR DEDGYFYIVD RKKDMIIVGG 430 440 450 460 470 480 YNVYPREVEE VLYSHPDVKE AVVIGVPDPQ SGEAVKGYVV PKRSGVTEED IMQHCEKHLA 490 500 510 KYKRPAAITF LDDIPKNATG KMLRRALRDI LPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene." Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S. Microbiology 144:859-875(1998) [PubMed: 9579061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 219.
[4]	"Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation." Matsuoka H., Hirooka K., Fujita Y. J. Biol. Chem. 282:5180-5194(2007) [PubMed: 17189250] [Abstract] Cited for: GENE NAME, INDUCTION. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y14083 Genomic DNA. Translation: CAA74533.1. AL009126 Genomic DNA. Translation: CAB12867.2.
PIR	A69831.
RefSeq	NP_388908.2.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Genome annotation databases
GeneID	939308.
GenomeReviews	Gene locus BSU10270 in contig AL009126_GR.
KEGG	bsu:BSU10270.
NMPDR	fig|224308.1.peg.1027.
Organism-specific databases
SubtiList	BG13057. lcfB. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
Enzyme and pathway databases
BioCyc	SUBTI:BSU10270-MONOMER.
Family and domain databases
InterPro	IPR020459. AMP-binding. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PRINTS	PR00154. AMPBINDING.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCFB_BACSU

Accession

Primary (citable) accession number: O07610
Secondary accession number(s): Q796T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	June 16, 2009
Last modified:	January 19, 2010
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)