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O07560 (LEPV_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal peptidase I V

Short name=SPase I
EC=3.4.21.89
Alternative name(s):
Leader peptidase I
Gene names
Name:sipV
Synonyms:yhjF
Ordered Locus Names:BSU10490
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length168 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.

Subcellular location

Cell membrane; Single-pass type II membrane protein Potential.

Induction

Expressed constitutively.

Miscellaneous

B.subtilis contains five chromosomal type I signal peptidases: SipS, SipT, SipU, SipV and SipW. They have different, but overlapping, substrate specificities and have different transcription patterns.

Sequence similarities

Belongs to the peptidase S26 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionHydrolase
Protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type peptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 168168Signal peptidase I V
PRO_0000109502

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2620Helical; Potential
Topological domain27 – 168142Extracellular Potential

Sites

Active site341 By similarity
Active site751 By similarity

Sequences

Sequence LengthMass (Da)Tools
O07560 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: D70BD09545638604

FASTA16818,956
        10         20         30         40         50         60 
MKKRFWFLAG VVSVVLAIQV KNAVFIDYKV EGVSMNPTFQ EGNELLVNKF SHRFKTIHRF 

        70         80         90        100        110        120 
DIVLFKGPDH KVLIKRVIGL PGETIKYKDD QLYVNGKQVA EPFLKHLKSV SAGSHVTGDF 

       130        140        150        160 
SLKDVTGTSK VPKGKYFVVG DNRIYSFDSR HFGPIREKNI VGVISDAE 

« Hide

References

« Hide 'large scale' references
[1]"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene."
Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S.
Microbiology 144:859-875(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Protein secretion and possible roles for multiple signal peptidases for precursor processing in bacilli."
Bron S., Bolhuis A., Tjalsma H., Holsappel S., Venema G., van Dijl J.M.
J. Biotechnol. 64:3-13(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y14081 Genomic DNA. Translation: CAA74468.1.
AL009126 Genomic DNA. Translation: CAB12889.1.
PIRA69708.
RefSeqNP_388930.1. NC_000964.3.

3D structure databases

ProteinModelPortalO07560.
SMRO07560. Positions 26-78.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU10490.

Protein family/group databases

MEROPSS26.006.

Proteomic databases

PaxDbO07560.

Protocols and materials databases

DNASU939318.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12889; CAB12889; BSU10490.
GeneID939318.
KEGGbsu:BSU10490.
PATRIC18973800. VBIBacSub10457_1094.

Organism-specific databases

GenoListBSU10490. [Micado]

Phylogenomic databases

eggNOGCOG0681.
HOGENOMHOG000003673.
KOK03100.
OMAFIDYKVE.
OrthoDBEOG622PW8.
PhylomeDBO07560.

Enzyme and pathway databases

BioCycBSUB:BSU10490-MONOMER.

Family and domain databases

Gene3D2.10.109.10. 2 hits.
InterProIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PANTHERPTHR12383. PTHR12383. 1 hit.
PfamPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSPR00727. LEADERPTASE.
SUPFAMSSF51306. SSF51306. 1 hit.
TIGRFAMsTIGR02227. sigpep_I_bact. 1 hit.
PROSITEPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEPV_BACSU
AccessionPrimary (citable) accession number: O07560
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList