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Protein

Signal peptidase I V

Gene

sipV

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341By similarity
Active sitei75 – 751By similarity

GO - Molecular functioni

  1. serine-type peptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BioCyciBSUB:BSU10490-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal peptidase I V (EC:3.4.21.89)
Short name:
SPase I
Alternative name(s):
Leader peptidase I
Gene namesi
Name:sipV
Synonyms:yhjF
Ordered Locus Names:BSU10490
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU10490. [Micado]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Transmembranei7 – 2620HelicalSequence AnalysisAdd
BLAST
Topological domaini27 – 168142ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 168168Signal peptidase I VPRO_0000109502Add
BLAST

Proteomic databases

PaxDbiO07560.

Expressioni

Inductioni

Expressed constitutively.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU10490.

Structurei

3D structure databases

ProteinModelPortaliO07560.
SMRiO07560. Positions 26-78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S26 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0681.
HOGENOMiHOG000003673.
InParanoidiO07560.
KOiK03100.
OMAiETIFIRD.
OrthoDBiEOG622PW8.
PhylomeDBiO07560.

Family and domain databases

Gene3Di2.10.109.10. 2 hits.
InterProiIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PfamiPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O07560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKRFWFLAG VVSVVLAIQV KNAVFIDYKV EGVSMNPTFQ EGNELLVNKF
60 70 80 90 100
SHRFKTIHRF DIVLFKGPDH KVLIKRVIGL PGETIKYKDD QLYVNGKQVA
110 120 130 140 150
EPFLKHLKSV SAGSHVTGDF SLKDVTGTSK VPKGKYFVVG DNRIYSFDSR
160
HFGPIREKNI VGVISDAE
Length:168
Mass (Da):18,956
Last modified:July 1, 1997 - v1
Checksum:iD70BD09545638604
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14081 Genomic DNA. Translation: CAA74468.1.
AL009126 Genomic DNA. Translation: CAB12889.1.
PIRiA69708.
RefSeqiNP_388930.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB12889; CAB12889; BSU10490.
GeneIDi939318.
KEGGibsu:BSU10490.
PATRICi18973800. VBIBacSub10457_1094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14081 Genomic DNA. Translation: CAA74468.1.
AL009126 Genomic DNA. Translation: CAB12889.1.
PIRiA69708.
RefSeqiNP_388930.1. NC_000964.3.

3D structure databases

ProteinModelPortaliO07560.
SMRiO07560. Positions 26-78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU10490.

Proteomic databases

PaxDbiO07560.

Protocols and materials databases

DNASUi939318.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12889; CAB12889; BSU10490.
GeneIDi939318.
KEGGibsu:BSU10490.
PATRICi18973800. VBIBacSub10457_1094.

Organism-specific databases

GenoListiBSU10490. [Micado]

Phylogenomic databases

eggNOGiCOG0681.
HOGENOMiHOG000003673.
InParanoidiO07560.
KOiK03100.
OMAiETIFIRD.
OrthoDBiEOG622PW8.
PhylomeDBiO07560.

Enzyme and pathway databases

BioCyciBSUB:BSU10490-MONOMER.

Family and domain databases

Gene3Di2.10.109.10. 2 hits.
InterProiIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PfamiPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene."
    Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S.
    Microbiology 144:859-875(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Protein secretion and possible roles for multiple signal peptidases for precursor processing in bacilli."
    Bron S., Bolhuis A., Tjalsma H., Holsappel S., Venema G., van Dijl J.M.
    J. Biotechnol. 64:3-13(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiLEPV_BACSU
AccessioniPrimary (citable) accession number: O07560
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: July 1, 1997
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

B.subtilis contains five chromosomal type I signal peptidases: SipS, SipT, SipU, SipV and SipW. They have different, but overlapping, substrate specificities and have different transcription patterns.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.