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Protein

Glutamate 5-kinase 2

Gene

proJ

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase 2 (proJ), Glutamate 5-kinase 1 (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATPUniRule annotation1
Binding sitei53SubstrateUniRule annotation1
Binding sitei140SubstrateUniRule annotation1
Binding sitei152Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi172 – 173ATPUniRule annotation2
Nucleotide bindingi214 – 220ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU18470-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinase 2UniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinase 2UniRule annotation
Short name:
GK 2UniRule annotation
Gene namesi
Name:proJ
Synonyms:yohA
Ordered Locus Names:BSU18470
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001096421 – 371Glutamate 5-kinase 2Add BLAST371

Proteomic databases

PaxDbiO07509
PRIDEiO07509

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100010176

Structurei

3D structure databases

ProteinModelPortaliO07509
SMRiO07509
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 356PUAUniRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246369
InParanoidiO07509
KOiK00931
OMAiPLHTRFI
PhylomeDBiO07509

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

O07509-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPDTSMKRV VVKIGSSSLT SLHGEISIRK LEALVDQVVK LKDAGYEVIL
60 70 80 90 100
VSSGAVAAGY RKLGFIQRPE KLPEKQASAS IGQGLLMEAY SKLFLAHGYV
110 120 130 140 150
ASQILITRSD FSDEYRYNNV RNTMNVLLER GIIPIINEND TVTVNRLKFG
160 170 180 190 200
DNDTLAAKVA GLIDADMLVI LSDIDGLYDG NPRTNPEAKK IQRVSEITPD
210 220 230 240 250
IEACAGDTGS IVGTGGMRSK LDAFKIVMAS GIKGFLGQAD AGDILYHAVH
260 270 280 290 300
EQAEGTYFEA EGTLPLNQKE QWIAFNSGPE GEMILSDDCS RKITNGQSSL
310 320 330 340 350
YLDGVQKIKG KFKSGSVVRL MDSKGTEIGL GIVNYSSVQL QEPEKKKELT
360 370
NRALIDQEAF VCHVDFSLPV N
Length:371
Mass (Da):40,354
Last modified:July 1, 1997 - v1
Checksum:i3352BFEFDECF703F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006720 Genomic DNA Translation: AAB62698.1
AL009126 Genomic DNA Translation: CAB13740.1
PIRiF69682
RefSeqiNP_389729.1, NC_000964.3
WP_003231456.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB13740; CAB13740; BSU18470
GeneIDi940098
KEGGibsu:BSU18470
PATRICifig|224308.179.peg.2014

Similar proteinsi

Entry informationi

Entry nameiPROJ_BACSU
AccessioniPrimary (citable) accession number: O07509
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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