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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH]

Gene

inhA

Organism
Mycobacterium avium
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Enoyl-ACP reductase of the type II fatty acid syntase (FAS-II) system, which is involved in the biosynthesis of mycolic acids, a major component of mycobacterial cell walls. Catalyzes the NADH-dependent reduction of the double bond of 2-trans-enoyl-[acyl-carrier protein], an essential step in the fatty acid elongation cycle of the FAS-II pathway. Shows preference for long-chain fatty acyl thioester substrates, and can also use 2-trans-enoyl-CoAs as alternative substrates. The mycobacterial FAS-II system utilizes the products of the FAS-I system as primers to extend fatty acyl chain lengths up to C56, forming the meromycolate chain that serves as the precursor for final mycolic acids.By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.By similarity

Pathwayi: mycolic acid biosynthesis

This protein is involved in the pathway mycolic acid biosynthesis, which is part of Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway mycolic acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei148May act as an intermediate that passes the hydride ion from NADH to the substrateBy similarity1
Binding sitei157SubstrateBy similarity1
Sitei157Transition state stabilizerBy similarity1
Binding sitei164NADBy similarity1
Binding sitei193NAD; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 21NADBy similarity2
Nucleotide bindingi64 – 65NADBy similarity2
Nucleotide bindingi95 – 96NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00915.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH]By similarity (EC:1.3.1.9By similarity)
Short name:
ENRBy similarity
Short name:
Enoyl-ACP reductaseBy similarity
Alternative name(s):
FAS-II enoyl-ACP reductaseBy similarity
NADH-dependent 2-trans-enoyl-ACP reductaseBy similarity
Gene namesi
Name:inhAImported
OrganismiMycobacterium avium
Taxonomic identifieri1764 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549141 – 268Enoyl-[acyl-carrier-protein] reductase [NADH]Add BLAST268

Interactioni

Subunit structurei

Homodimer. Homotetramer.By similarity

Protein-protein interaction databases

STRINGi262316.MAP1210.

Structurei

3D structure databases

ProteinModelPortaliO07400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CSJ. Bacteria.
COG0623. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 1 hit.
PTHR24322:SF317. PTHR24322:SF317. 1 hit.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

O07400-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLLDGKRI LVTGIITDSS IAFHIAKVAQ EAGAQLVLTG FDRLRLIQRI
60 70 80 90 100
VDRLPEKAPL IELDVQNEEH LNTLAQRVTG EIGEGNKLDG VVHSIASSET
110 120 130 140 150
GMADQPFFDA PYEDVSKGIH ISADSDASLA KALLPIMNPG GSIVGMDFDP
160 170 180 190 200
SRAMPAYNWM TVAKSALESV NRFVAREAGP HGVRSNLVAA GPIRTLAMAG
210 220 230 240 250
IVGGVLGDQA AEQIRLLEEG WDQRAPIGWN MKDPTPVAKT VCALLSDWLP
260
ATTGTIIYAD RGASTQLL
Length:268
Mass (Da):28,530
Last modified:July 1, 1997 - v1
Checksum:iF73501BD2B0F9990
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002133 Genomic DNA. Translation: AAC46204.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002133 Genomic DNA. Translation: AAC46204.1.

3D structure databases

ProteinModelPortaliO07400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262316.MAP1210.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CSJ. Bacteria.
COG0623. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00915.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 1 hit.
PTHR24322:SF317. PTHR24322:SF317. 1 hit.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiINHA_MYCAV
AccessioniPrimary (citable) accession number: O07400
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.