O07399 (FABG_MYCAV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG
EC=1.1.1.100

Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase

Gene names

Name:

fabG

Organism

Mycobacterium avium

Taxonomic identifier

1764 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis By similarity.
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	fatty acid elongation Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Inferred from sequence or structural similarity. Source: UniProtKB NADP binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	3-oxoacyl-[acyl-carrier-protein] reductase FabG		PRO_0000054675
Regions
	Nucleotide binding	30 – 33	4	NADP By similarity
	Nucleotide binding	69 – 70	2	NADP By similarity
	Nucleotide binding	161 – 165	5	NADP By similarity
Sites
	Active site	161	1	Proton acceptor By similarity
	Binding site	55	1	NADP By similarity
	Binding site	96	1	NADP; via carbonyl oxygen By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	194	1	NADP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O07399 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: C3A20AA0B6251655	FASTA	255	26,706
	10 20 30 40 50 60 MTDTATENTT ESAADYGRPA FVSRSVLVTG GNRGIGLAIA QRLAAEAHKV AVTHRGSGAP 70 80 90 100 110 120 DGLFGVECDV TDNDAVDRAF TEVEEHQGPV EVLVSNAGIS KDAFLIRMTE ERFTEVINAN 130 140 150 160 170 180 LTGAFRVTQR AARSMQKKRF GRIIYIGSVS GMWGIGNQAN YAAAKAGLIG MARSISRELS 190 200 210 220 230 240 KAGVTANVVA PGYIDTEMTR ALDERIQAGA LEFIPAKRVG TAAEVPGAVS FLASEDASYI 250 AGAVIPVDGG MGMGH

References

[1]

"Determination of a 15437 bp nucleotide sequence around the inhA gene of Mycobacterium avium and similarity analysis of the products of putative ORFs." Labo M., Gusberti L., de Rossi E., Speziale P., Riccardi G. Microbiology 144:807-814(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GIR10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF002133 Genomic DNA. Translation: AAC46203.1.
3D structure databases
ProteinModelPortal	O07399.
SMR	O07399. Positions 17-255.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG1028.
Enzyme and pathway databases
UniPathway	UPA00094.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABG_MYCAV

Accession

Primary (citable) accession number: O07399

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program