Reviewed, UniProtKB/Swiss-Prot O07344 (LEP_STRPN)

Names and origin

Protein names

Recommended name:
    Signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Synonyms:	spi
Ordered Locus Names:	SP_0402

Organism

Streptococcus pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Signal peptidase I		PRO_0000109533
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 30	20	Potential
	Topological domain	31 – 204	174	Extracellular Potential
Sites
	Active site	38	1	By similarity
	Active site	76	1	By similarity
Experimental info
	Sequence conflict	3	1	S → L in AAB69116. Ref.1
	Sequence conflict	195	1	L → F in AAB69116. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O07344-1 [UniParc]. Last modified September 26, 2001. Version 2. Checksum: 2B3CDCF1A070C4B9	FASTA	204	23,438
	10 20 30 40 50 60 MNSFKNFLKE WGLFLLILSL LALSRIFFWS NVRVEGHSMD PTLADGEILF VVKHLPIDRF 70 80 90 100 110 120 DIVVAHEEDG NKDIVKRVIG MPGDTIRYEN DKLYINDKET DEPYLADYIK RFKDDKLQST 130 140 150 160 170 180 YSGKGFEGNK GTFFRSIAQK AQAFTVDVNY NTNFSFTVPE GEYLLLGDDR LVSSDSRHVG 190 200 TFKAKDITGE AKFRLWPITR IGTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a Streptococcus pneumoniae gene encoding signal peptidase I and overproduction of the enzyme." Zhang Y.-B., Greenberg B., Lacks S.A. Gene 194:249-255(1997) [PubMed: 9272867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 533.
[2]	"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U90721 Genomic DNA. Translation: AAB69116.1. AE005672 Genomic DNA. Translation: AAK74565.1.
PIR	D95046. D97917.
RefSeq	NP_344925.1.
3D structure databases
HSSP	HSSP built from PDB template 1B12 based on UniProtKB P00803.
ModBase	Search...
Genome annotation databases
GeneID	930336.
GenomeReviews	Gene locus SP_0402 in contig AE005672_GR.
KEGG	spn:SP_0402.
TIGR	SP_0402.
Phylogenomic databases
HOGENOM	O07344.
OMA	O07344. DEYLAAF.
Enzyme and pathway databases
BRENDA	3.4.21.89. 600.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_STRPN

Accession

Primary (citable) accession number: O07344

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	September 26, 2001
Last modified:	June 16, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)