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O07299 (ALGD_PSESH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GDP-mannose 6-dehydrogenase
Short name=GMD
EC=1.1.1.132
Gene names
Name:algD
OrganismPseudomonas syringae pv. phaseolicola
Taxonomic identifier319 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics By similarity.

Catalytic activity

GDP-D-mannose + 2 NAD+ + H2O = GDP-D-mannuronate + 2 NADH.

Pathway

Glycan biosynthesis; alginate biosynthesis.

Sequence similarities

Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
   Biological processAlginate biosynthesis
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processalginic acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionGDP-mannose 6-dehydrogenase activity

Inferred from electronic annotation. Source: EC

NAD binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438GDP-mannose 6-dehydrogenase
PRO_0000074069

Sites

Active site2681 By similarity
Site101NAD binding By similarity
Site111NAD binding By similarity
Site301NAD binding By similarity
Site351NAD binding By similarity
Site861NAD binding By similarity
Site1611NAD binding By similarity
Site2711NAD binding By similarity
Site3311NAD binding By similarity

Sequences

Sequence LengthMass (Da)Tools
O07299 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 6A4EDC8BC55BCC8F

FASTA43847,595
        10         20         30         40         50         60 
MRISIFGLGY VGAVCAGCLS ARGHDVVGVD ISSTKIDLIN NGKSPIVEPG LEELLQKGIS 

        70         80         90        100        110        120 
TGKLRGTTDF AEAIRATDLS MICVGTPSKK NGDLELDYIE SVCREIGYVL RDKATRHTIV 

       130        140        150        160        170        180 
VRSTVLPGTV ANVVIPILED CSGKKAGVDF GVAVNPEFLR ESTAIKDYDL PPMTVIGEFD 

       190        200        210        220        230        240 
KASGDVLQSL YEELDAPIIR KDIAVAEMIK YTCNVWHATK VTFANEIGNI AKAVGVDGRE 

       250        260        270        280        290        300 
VMDVVCQDKA LNLSQYYMRP GFAFGGSCLP KDVRALTYRA SSLDVEAPLL NSLMRSNTSQ 

       310        320        330        340        350        360 
VQNAFDMVAS YDTRKVALLG LSFKAGTDDL RESPLVELAE MLIGKGFDLS IFDSNVEYAR 

       370        380        390        400        410        420 
VHGANKDYIE SKIPHVSSLL NSDFDQVIND SDVIILGNRD ERFRALANKT PEGKRVIDLV 

       430 
GFMTNATSED GRAEGICW

« Hide

References

[1]"Isolation and characterization of the algD gene from Pseudomonas syringae pv. phaseolicola and distribution among other pseudomonads and related organisms."
Koopmann B., Noellenburg M., Rudolph K.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S2-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF001555 Genomic DNA. Translation: AAB58939.1.

3D structure databases

ProteinModelPortalO07299.
SMRO07299. Positions 1-438.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00286.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. Nucleotide_sugar_DH.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
PfamPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMSSF48179. 6DGDH_C_like. 1 hit.
SSF52413. UDP-Glc/GDP-Man_DH_C. 1 hit.
TIGRFAMsTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameALGD_PSESH
AccessionPrimary (citable) accession number: O07299
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: March 6, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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