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Protein

GDP-mannose 6-dehydrogenase

Gene

algD

Organism
Pseudomonas syringae pv. phaseolicola
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics (By similarity).By similarity

Catalytic activityi

GDP-D-mannose + 2 NAD+ + H2O = GDP-D-mannuronate + 2 NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 101NAD bindingBy similarity
Sitei11 – 111NAD bindingBy similarity
Sitei30 – 301NAD bindingBy similarity
Sitei35 – 351NAD bindingBy similarity
Sitei86 – 861NAD bindingBy similarity
Sitei161 – 1611NAD bindingBy similarity
Active sitei268 – 2681By similarity
Sitei271 – 2711NAD bindingBy similarity
Sitei331 – 3311NAD bindingBy similarity

GO - Molecular functioni

  1. GDP-mannose 6-dehydrogenase activity Source: UniProtKB-EC
  2. NAD binding Source: InterPro

GO - Biological processi

  1. alginic acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Alginate biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00286.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-mannose 6-dehydrogenase (EC:1.1.1.132)
Short name:
GMD
Gene namesi
Name:algD
OrganismiPseudomonas syringae pv. phaseolicola
Taxonomic identifieri319 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438GDP-mannose 6-dehydrogenasePRO_0000074069Add
BLAST

Structurei

3D structure databases

SMRiO07299. Positions 1-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR028358. GDPman_DH.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500135. GDPman_DH. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

Sequencei

Sequence statusi: Complete.

O07299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRISIFGLGY VGAVCAGCLS ARGHDVVGVD ISSTKIDLIN NGKSPIVEPG
60 70 80 90 100
LEELLQKGIS TGKLRGTTDF AEAIRATDLS MICVGTPSKK NGDLELDYIE
110 120 130 140 150
SVCREIGYVL RDKATRHTIV VRSTVLPGTV ANVVIPILED CSGKKAGVDF
160 170 180 190 200
GVAVNPEFLR ESTAIKDYDL PPMTVIGEFD KASGDVLQSL YEELDAPIIR
210 220 230 240 250
KDIAVAEMIK YTCNVWHATK VTFANEIGNI AKAVGVDGRE VMDVVCQDKA
260 270 280 290 300
LNLSQYYMRP GFAFGGSCLP KDVRALTYRA SSLDVEAPLL NSLMRSNTSQ
310 320 330 340 350
VQNAFDMVAS YDTRKVALLG LSFKAGTDDL RESPLVELAE MLIGKGFDLS
360 370 380 390 400
IFDSNVEYAR VHGANKDYIE SKIPHVSSLL NSDFDQVIND SDVIILGNRD
410 420 430
ERFRALANKT PEGKRVIDLV GFMTNATSED GRAEGICW
Length:438
Mass (Da):47,595
Last modified:June 30, 1997 - v1
Checksum:i6A4EDC8BC55BCC8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001555 Genomic DNA. Translation: AAB58939.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001555 Genomic DNA. Translation: AAB58939.1.

3D structure databases

SMRiO07299. Positions 1-438.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00286.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR028358. GDPman_DH.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500135. GDPman_DH. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of the algD gene from Pseudomonas syringae pv. phaseolicola and distribution among other pseudomonads and related organisms."
    Koopmann B., Noellenburg M., Rudolph K.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S2-1.

Entry informationi

Entry nameiALGD_PSESH
AccessioniPrimary (citable) accession number: O07299
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 1998
Last sequence update: June 30, 1997
Last modified: March 31, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.