O07247 (DCD_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxycytidine triphosphate deaminase
Short name=dCTP deaminase
EC=3.5.4.13

Gene names

Name:	dcd
Ordered Locus Names:	Rv0321, MT0336
ORF Names:	MTCY63.26

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	dCTP + H2O = dUTP + NH3. HAMAP MF_00146
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2. HAMAP MF_00146
Sequence similarities	Belongs to the dCTP deaminase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	dUTP biosynthetic process Inferred from electronic annotation. Source: InterPro protein homotrimerization Inferred from physical interaction. Source: MTBBASE
Molecular function	dCTP deaminase (dUMP-forming) activity Inferred from direct assay. Source: MTBBASE dCTP deaminase activity Inferred from electronic annotation. Source: EC dUTP diphosphatase activity Inferred from direct assay. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 190

190

Deoxycytidine triphosphate deaminase HAMAP MF_00146

PRO_0000155997

Secondary structure

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190

Helix Strand Turn

Details...

Helix

5 – 13

9

Beta strand

16 – 21

6

Helix

24 – 26

3

Beta strand

31 – 36

6

Beta strand

40 – 43

4

Helix

58 – 60

3

Beta strand

61 – 64

4

Beta strand

72 – 74

3

Beta strand

79 – 89

11

Beta strand

94 – 99

6

Helix

102 – 105

4

Turn

106 – 108

3

Beta strand

109 – 112

4

Beta strand

127 – 132

6

Beta strand

134 – 136

3

Beta strand

138 – 140

3

Beta strand

144 – 156

13

Turn

165 – 168

4

Turn

182 – 185

4

Sequences

	Sequence		Length	Mass (Da)
	O07247 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: F409329810B64781	FASTA	190	20,870
	10 20 30 40 50 60 MLLSDRDLRA EISSGRLGID PFDDTLVQPS SIDVRLDCLF RVFNNTRYTH IDPAKQQDEL 70 80 90 100 110 120 TSLVQPVDGE PFVLHPGEFV LGSTLELFTL PDNLAGRLEG KSSLGRLGLL THSTAGFIDP 130 140 150 160 170 180 GFSGHITLEL SNVANLPITL WPGMKIGQLC MLRLTSPSEH PYGSSRAGSK YQGQRGPTPS 190 RSYQNFIRST

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842573 Genomic DNA. Translation: CAB09605.1.
AE000516 Genomic DNA. Translation: AAK44559.1.

PIR

B70526.

RefSeq

NP_214835.1. NC_000962.2.
NP_334745.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QLP	X-ray	2.47	A/B/C/D/E/F	1-161	[»]
2QXX	X-ray	2.00	A/B	1-190	[»]

ProteinModelPortal

O07247.

SMR

O07247. Positions 1-189.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000001002; EBMYCP00000001002; EBMYCG00000001002.
EBMYCT00000070760; EBMYCP00000068819; EBMYCG00000070755.

GeneID

886552.
923426.

GenomeReviews

Gene locus MT0336 in contig AE000516_GR.
Gene locus Rv0321 in contig AL123456_GR.

KEGG

mtc:MT0336.
mtu:Rv0321.

PATRIC

18122451. VBIMycTub22151_0360.

TIGR

MT0336.

Organism-specific databases

TubercuList

Rv0321.

Phylogenomic databases

GeneTree

EBGT00050000016475.

HOGENOM

HBG553199.

OMA

MEYFRIP.

PhylomeDB

O07247.

ProtClustDB

PRK00416.

Family and domain databases

HAMAP

MF_00146. dCTP_deaminase.
[Tree]

InterPro

IPR011962. dCTP_deam.
IPR008180. dUTP_pyroPase.
[Graphical view]

KO

K01494.

Pfam

PF00692. dUTPase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02274. DCTP_deam. 1 hit.

ProtoNet

Search...

Entry information

Entry name

DCD_MYCTU

Accession

Primary (citable) accession number: O07247

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program